calculating the structure-based phylogenetic relationship
... homologs. Additionally, polypeptide sequences contain sparse information about a protein relative to that of other biochemical properties such as structure or function. Because protein ...
... homologs. Additionally, polypeptide sequences contain sparse information about a protein relative to that of other biochemical properties such as structure or function. Because protein ...
BACILLUS SPHAERICUS TOXINS: Molecular Biology and Mode of
... sverval high-toxicity strains. They appear to be organized in an operon with a 174- to 176-base pair (bp) intergenic region. A stem-loop structure (characteristic of transcription terminators) lies downstream from the P42 gene (figure 2a) (8, 46). No sequence similar to Bacillus subtilis sporulation ...
... sverval high-toxicity strains. They appear to be organized in an operon with a 174- to 176-base pair (bp) intergenic region. A stem-loop structure (characteristic of transcription terminators) lies downstream from the P42 gene (figure 2a) (8, 46). No sequence similar to Bacillus subtilis sporulation ...
A PEST-like Sequence in the N-Terminal Cytoplasmic Domain of
... and endocytosis targeting signals in several yeast membrane proteins. These include the SINNDAKSS motif in the C-terminal cytoplasmic domain of the Ste2 R-factor receptor (6, 7). Deletion analysis demonstrated that the middlespanning linker region of the ABC transporter, Ste6p, contains a signal tha ...
... and endocytosis targeting signals in several yeast membrane proteins. These include the SINNDAKSS motif in the C-terminal cytoplasmic domain of the Ste2 R-factor receptor (6, 7). Deletion analysis demonstrated that the middlespanning linker region of the ABC transporter, Ste6p, contains a signal tha ...
An Investigation of Acetobacter aceti N5
... the role of two conserved histidines. A pH-rate comparison of AaPurE and the active mutant AaPurE-H59D was used to identify His59 as the key active site acid/base residue. The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was fo ...
... the role of two conserved histidines. A pH-rate comparison of AaPurE and the active mutant AaPurE-H59D was used to identify His59 as the key active site acid/base residue. The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was fo ...
LNBI 9043 - Lupin Allergy: Uncovering Structural Features and
... in traditional foods, the number of reports of allergic reactions to lupin proteins is also rising, either as primary lupin allergy or as a result of cross-reactivity to other legume proteins, particularly peanut, soybean, lentil, bean, chickpea, and pea [4]. The most common clinical pattern of lupi ...
... in traditional foods, the number of reports of allergic reactions to lupin proteins is also rising, either as primary lupin allergy or as a result of cross-reactivity to other legume proteins, particularly peanut, soybean, lentil, bean, chickpea, and pea [4]. The most common clinical pattern of lupi ...
Prokaryotic proteins of antioxidant defense in Trichomonas vaginalis
... pivotal driving force for ATP synthesis within most aerobic eukaryotic cells. In the process of oxidative phosphorylation, some electrons “leak” to oxygen prematurely, forming the anion and free radical, superoxide O2-. Superoxide causes the release of iron from iron-containing clusters of enzymes ( ...
... pivotal driving force for ATP synthesis within most aerobic eukaryotic cells. In the process of oxidative phosphorylation, some electrons “leak” to oxygen prematurely, forming the anion and free radical, superoxide O2-. Superoxide causes the release of iron from iron-containing clusters of enzymes ( ...
The Genera Staphylococcus and Macrococcus
... cultures are considered to be S. aureus. Test positive and negative controls simultaneously with cultures of unknown coagulase reactivity. Recheck doubtful coagulase test results on BHI cultures which have been incubated at 35–37°C for > 18 but ≤ 48 h. This procedure is recommended by the AOAC for t ...
... cultures are considered to be S. aureus. Test positive and negative controls simultaneously with cultures of unknown coagulase reactivity. Recheck doubtful coagulase test results on BHI cultures which have been incubated at 35–37°C for > 18 but ≤ 48 h. This procedure is recommended by the AOAC for t ...
Safety assessment - Federal Register of Legislation
... Monsanto Australia Limited is seeking approval in the Australia New Zealand Food Standards Code (the Code) for food derived from corn line 88017 (MON 88017), a variety that has been genetically modified (GM) for insect-protection and herbicide-tolerance. Standard 1.5.2 – Food Produced Using Gene Tec ...
... Monsanto Australia Limited is seeking approval in the Australia New Zealand Food Standards Code (the Code) for food derived from corn line 88017 (MON 88017), a variety that has been genetically modified (GM) for insect-protection and herbicide-tolerance. Standard 1.5.2 – Food Produced Using Gene Tec ...
Short-Term Overexpression of a Constitutively Active Form of AMP
... AMPK in drug development. Studies in animal models of type 2 diabetes have shown that the pharmacological activation of AMPK with 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) or metformin decreases blood glucose levels and improves lipid profile (7–9). However, these compounds are not ideal ...
... AMPK in drug development. Studies in animal models of type 2 diabetes have shown that the pharmacological activation of AMPK with 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) or metformin decreases blood glucose levels and improves lipid profile (7–9). However, these compounds are not ideal ...
Autotrophic CO2 fixation via the reductive tricarboxylic acid cycle in
... in A. aeolicus and Tc. ruber. Based either on the whole genome sequence (A. aeolicus) or carbon isotopic measurements (Tc. ruber) both organisms were previously suspected to use this pathway, but direct evidence had been lacking (Deckert et al., 1998; Jahnke et al., 2001). Isocitrate dehydrogenase, ...
... in A. aeolicus and Tc. ruber. Based either on the whole genome sequence (A. aeolicus) or carbon isotopic measurements (Tc. ruber) both organisms were previously suspected to use this pathway, but direct evidence had been lacking (Deckert et al., 1998; Jahnke et al., 2001). Isocitrate dehydrogenase, ...
Gene Section LRP5 (low density lipoprotein receptor related protein 5)
... mutation in the LRP5 gene (G171V; 603506.0013) that results in an autosomal dominant high bone mass trait. Van Wesenbeeck et al. performed mutation analysis of the LRP5 gene in 10 families or isolated patients with various conditions of an increased bone density, including endosteal hyperostosis. Di ...
... mutation in the LRP5 gene (G171V; 603506.0013) that results in an autosomal dominant high bone mass trait. Van Wesenbeeck et al. performed mutation analysis of the LRP5 gene in 10 families or isolated patients with various conditions of an increased bone density, including endosteal hyperostosis. Di ...
Enzymatic activation of sulfur for incorporation into biomolecules in
... inactivated (Zheng et al., 1998). The iscS gene of A. vinelandii is located at the 5 0 end of an operon, which also contains iscU, iscA, hscB, hscA and fdx; this type of operon is widespread in nature (Zheng et al., 1998) and crucial for general iron sulfur cluster (isc) biosynthesis in many organis ...
... inactivated (Zheng et al., 1998). The iscS gene of A. vinelandii is located at the 5 0 end of an operon, which also contains iscU, iscA, hscB, hscA and fdx; this type of operon is widespread in nature (Zheng et al., 1998) and crucial for general iron sulfur cluster (isc) biosynthesis in many organis ...
Role of adiponectin in the regulation of carbohydrate and lipid
... 17. Maeda N, Takahashi M, Funahashi T, et al. PPAR gamma ligands increase expression and plasma concentrations of adiponectin, an adipose-derived protein. Diabetes 2001; 50: 2094-2099. 18. Fasshauer M, Klein J, Neumann S, Eszlinger M, Paschke R. Hormonal regulation of adiponectin gene expression in ...
... 17. Maeda N, Takahashi M, Funahashi T, et al. PPAR gamma ligands increase expression and plasma concentrations of adiponectin, an adipose-derived protein. Diabetes 2001; 50: 2094-2099. 18. Fasshauer M, Klein J, Neumann S, Eszlinger M, Paschke R. Hormonal regulation of adiponectin gene expression in ...
Dateien anzeigen - Universität Düsseldorf
... metabolism of G. oxydans also poses a problem, which is the low cell yield resulting in high costs for biomass production. This study aimed at an increase of the cell yield of G. oxydans on glucose in order to improve its application potential. For this purpose, prevention of incomplete glucose oxid ...
... metabolism of G. oxydans also poses a problem, which is the low cell yield resulting in high costs for biomass production. This study aimed at an increase of the cell yield of G. oxydans on glucose in order to improve its application potential. For this purpose, prevention of incomplete glucose oxid ...
167 renal and small intestinal sodium
... around 40–70 mmol l21. At neutral pH, interaction with sodium is sigmoidal, with a Hill coefficient of approximately 2, suggesting a stoichiometry of 2Na+/HPO422 (for a review, see Murer et al. 1991). With respect to the interactions of Pi and Na+, apical Na+/Pi symport in small intestine (duodenum) ...
... around 40–70 mmol l21. At neutral pH, interaction with sodium is sigmoidal, with a Hill coefficient of approximately 2, suggesting a stoichiometry of 2Na+/HPO422 (for a review, see Murer et al. 1991). With respect to the interactions of Pi and Na+, apical Na+/Pi symport in small intestine (duodenum) ...
EP 1790660 B1
... [0020] The method will typically involve the steps of: obtaining nucleic acid encoding a protein of the invention; manipulating said nucleic acid to remove at least one domain from within the protein. The resulting nucleic acid may be inserted into an expression vector, or may already be part of an ...
... [0020] The method will typically involve the steps of: obtaining nucleic acid encoding a protein of the invention; manipulating said nucleic acid to remove at least one domain from within the protein. The resulting nucleic acid may be inserted into an expression vector, or may already be part of an ...
Desert hedgehog is a mammal-specific gene expressed during testicular and ovarian
... humans but is not present in the avian lineage. Like other hedgehog proteins, DHH signals through the patched (PTCH) receptors 1 and 2. Here we examine the expression and protein distribution of DHH, PTCH1 and PTCH2 in the developing testes of a marsupial mammal (the tammar wallaby) to determine whe ...
... humans but is not present in the avian lineage. Like other hedgehog proteins, DHH signals through the patched (PTCH) receptors 1 and 2. Here we examine the expression and protein distribution of DHH, PTCH1 and PTCH2 in the developing testes of a marsupial mammal (the tammar wallaby) to determine whe ...
Desert hedgehogis a mammal-specific gene expressed during
... humans but is not present in the avian lineage. Like other hedgehog proteins, DHH signals through the patched (PTCH) receptors 1 and 2. Here we examine the expression and protein distribution of DHH, PTCH1 and PTCH2 in the developing testes of a marsupial mammal (the tammar wallaby) to determine whe ...
... humans but is not present in the avian lineage. Like other hedgehog proteins, DHH signals through the patched (PTCH) receptors 1 and 2. Here we examine the expression and protein distribution of DHH, PTCH1 and PTCH2 in the developing testes of a marsupial mammal (the tammar wallaby) to determine whe ...
Staphylococcus haemolyticus lipase
... Fig. 4. Hydrolytic activity of the lipase L62 for various lipids. A: The hydrolytic activity of the lipase L62 for various chain lengths of triglycerides, 1,3-rac-diolein (1,3-DO), and 1-rac-monoolein (1-MO), was measured by the pH-stat method using each substrate emulsion (20 mM) prepared as descri ...
... Fig. 4. Hydrolytic activity of the lipase L62 for various lipids. A: The hydrolytic activity of the lipase L62 for various chain lengths of triglycerides, 1,3-rac-diolein (1,3-DO), and 1-rac-monoolein (1-MO), was measured by the pH-stat method using each substrate emulsion (20 mM) prepared as descri ...
Yeast lipid metabolism at a glance
... Tamura et al., 2012). Therefore, a lipid transport system must exist to translocate lipids from their site of synthesis to their final destination (Achleitner et al., 1999; Schnabl et al., 2005; Peretti et al., 2008). Due to the very well-conserved mechanisms between all eukaryotes and due to other ...
... Tamura et al., 2012). Therefore, a lipid transport system must exist to translocate lipids from their site of synthesis to their final destination (Achleitner et al., 1999; Schnabl et al., 2005; Peretti et al., 2008). Due to the very well-conserved mechanisms between all eukaryotes and due to other ...
Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3
... Background: Campylobacter jejuni and C. coli share a multitude of risk factors associated with human gastrointestinal disease, yet their phylogeny differs significantly. C. jejuni is scattered into several lineages, with no apparent linkage, whereas C. coli clusters into three distinct phylogenetic ...
... Background: Campylobacter jejuni and C. coli share a multitude of risk factors associated with human gastrointestinal disease, yet their phylogeny differs significantly. C. jejuni is scattered into several lineages, with no apparent linkage, whereas C. coli clusters into three distinct phylogenetic ...
Nucleotide sequence of the Tn10 encoded tetracycline resistance
... assays, encoded by a gene which runs in the opposite direction to that encoding the repressor [2]. In addition, a tetracyclineinducible, low molecular weight protein has been detected on R222, a resistance factor bearing Tn10 [5]. Recently, genetic evidence was provided for the existence of two gene ...
... assays, encoded by a gene which runs in the opposite direction to that encoding the repressor [2]. In addition, a tetracyclineinducible, low molecular weight protein has been detected on R222, a resistance factor bearing Tn10 [5]. Recently, genetic evidence was provided for the existence of two gene ...
Glutamate Dehydrogenases: Enzymology, Physiological
... of purines, pyrimidines, amino sugars, histidine, tryptophan, asparagine, NAD and paminobenzoate. Therefore glutamate is a key element in the nitrogen flow, as it plays a role of nitrogen donor and acceptor. Glutamate can be synthesized by two alternative routes: one involves catalysis of GDH in the ...
... of purines, pyrimidines, amino sugars, histidine, tryptophan, asparagine, NAD and paminobenzoate. Therefore glutamate is a key element in the nitrogen flow, as it plays a role of nitrogen donor and acceptor. Glutamate can be synthesized by two alternative routes: one involves catalysis of GDH in the ...
53 - Lab Times
... att-sequences. Once cloned into this Gateway entry clone vector, DNA segments can be rapidly transferred into diverse Gateway expression vectors via a similar Clonasebased reaction. In recent years, a couple of labs have established extensive collections of ORF clones created by Gateway cloning cont ...
... att-sequences. Once cloned into this Gateway entry clone vector, DNA segments can be rapidly transferred into diverse Gateway expression vectors via a similar Clonasebased reaction. In recent years, a couple of labs have established extensive collections of ORF clones created by Gateway cloning cont ...