Molecular and General Genetics.
... tryptophan produced IAA if tryptophan was present but did not synthesise IAA from indole. Similar results were obtained for a mutant strain with additional mutations in the genes ipdC and trpD . This suggests the existence of an alternative Trp-dependent route for IAA synthesis. On gluconate as a ca ...
... tryptophan produced IAA if tryptophan was present but did not synthesise IAA from indole. Similar results were obtained for a mutant strain with additional mutations in the genes ipdC and trpD . This suggests the existence of an alternative Trp-dependent route for IAA synthesis. On gluconate as a ca ...
Attachment 1 - Food Standards Australia New Zealand
... New Zealand Food Standards Code (the Code) to approve food derived from genetically modified (GM) herbicide-tolerant soybean MON 89788. Standard 1.5.2 – Food Produced using Gene Technology, requires that GM foods undergo a pre-market safety assessment before they may be sold in Australia and New Zea ...
... New Zealand Food Standards Code (the Code) to approve food derived from genetically modified (GM) herbicide-tolerant soybean MON 89788. Standard 1.5.2 – Food Produced using Gene Technology, requires that GM foods undergo a pre-market safety assessment before they may be sold in Australia and New Zea ...
The semi-phosphorylative Entner–Doudoroff pathway in
... KD(P)G aldolase and GAPN of T. tenax [32]. The standard assay was performed in 100 mM Hepes/KOH (pH 7.0, 70 ◦C) in the presence of KDG kinase (1.5 and 3 µg of T. tenax protein after gel filtration; 40 and 80 µg of S. solfataricus protein after heat precipitation), 2 mM ATP, 2 mM MgCl2 , 10 mM NAD+ , ...
... KD(P)G aldolase and GAPN of T. tenax [32]. The standard assay was performed in 100 mM Hepes/KOH (pH 7.0, 70 ◦C) in the presence of KDG kinase (1.5 and 3 µg of T. tenax protein after gel filtration; 40 and 80 µg of S. solfataricus protein after heat precipitation), 2 mM ATP, 2 mM MgCl2 , 10 mM NAD+ , ...
Nature inspired platforms for production of acetyl
... isopropanol has been reported by two groups using similar biosynthetic schemes with genes from either Clostridium acetobutylicum (thl, ctfAB, and adc) or E. coli (atoAD) to convert acetyl-CoA to acetone through acetoacetyl-CoA and acetoacetate16,17 (Figure 2). Meanwhile, an alcohol dehydrogenase gen ...
... isopropanol has been reported by two groups using similar biosynthetic schemes with genes from either Clostridium acetobutylicum (thl, ctfAB, and adc) or E. coli (atoAD) to convert acetyl-CoA to acetone through acetoacetyl-CoA and acetoacetate16,17 (Figure 2). Meanwhile, an alcohol dehydrogenase gen ...
Identification and expression of GH
... 29 different serovar strains of B. thuringiensis and they were expressed in Escherichia coli. The ORFs of the chitosanases contained 1359 nucleotides and the protein products had high levels of sequence identity (496%) to other Bacillus species GH-8 chitosanases. Thin-layer chromatography and HPLC a ...
... 29 different serovar strains of B. thuringiensis and they were expressed in Escherichia coli. The ORFs of the chitosanases contained 1359 nucleotides and the protein products had high levels of sequence identity (496%) to other Bacillus species GH-8 chitosanases. Thin-layer chromatography and HPLC a ...
Biochemical, biophysical and interaction studies of the stress
... advice on PhD related issues and CD experiments. Also I would like to thank Dr Marija Kristic Demonacos for her help when carrying out the molecular cloning aspect of this project and letting me work on her bench space, also providing me with an hSTRAP construct (pHA1-hSTRAP(1-440)) which was used i ...
... advice on PhD related issues and CD experiments. Also I would like to thank Dr Marija Kristic Demonacos for her help when carrying out the molecular cloning aspect of this project and letting me work on her bench space, also providing me with an hSTRAP construct (pHA1-hSTRAP(1-440)) which was used i ...
Analysis of the genetic potential of the spongederived fungus
... Preparation of high quality plasmid DNA from E. coli ........................................................... - 187 Cloning of PCR products with the pGEM-T Easy Vector System ........................................... - 187 Preparation of the TA cloning vector ................................... ...
... Preparation of high quality plasmid DNA from E. coli ........................................................... - 187 Cloning of PCR products with the pGEM-T Easy Vector System ........................................... - 187 Preparation of the TA cloning vector ................................... ...
4. characterisation of novel proteins
... generations confirmed stability of the introduced genetic elements and indicated that the genes are inherited in a predicted manner according to Mendelian principles. Bioinformatic analysis of the insertion site and associated junction regions did not indicate any likelihood of unexpected gene produ ...
... generations confirmed stability of the introduced genetic elements and indicated that the genes are inherited in a predicted manner according to Mendelian principles. Bioinformatic analysis of the insertion site and associated junction regions did not indicate any likelihood of unexpected gene produ ...
ABA overlysensitive5 (ABO5), encoding a pentatricopeptide repeat
... genes. Plant mitochondria have specific alternative respiratory pathways, including the non-proton-pumping NAD(P)H dehydrogenases, which bypass complex I for diminishing ROS production, and an alternative oxidase (AOX), which directly accepts electrons from the ubiquinone pool for reducing ROS produ ...
... genes. Plant mitochondria have specific alternative respiratory pathways, including the non-proton-pumping NAD(P)H dehydrogenases, which bypass complex I for diminishing ROS production, and an alternative oxidase (AOX), which directly accepts electrons from the ubiquinone pool for reducing ROS produ ...
Functional characterisation of the TUP5 gene in - diss.fu
... mutants of which hypocotyl and root explants were cultivated on medium containing low auxin and cytokinin concentrations. The phytohormone concentrations were under the threshold of callus induction in the wild type, while the tumor prone mutants produced calli (Riefler, 2001). Furthermore, tup5-1 a ...
... mutants of which hypocotyl and root explants were cultivated on medium containing low auxin and cytokinin concentrations. The phytohormone concentrations were under the threshold of callus induction in the wild type, while the tumor prone mutants produced calli (Riefler, 2001). Furthermore, tup5-1 a ...
Potassium starvation responses in yeast highlight novel potassium-related functions
... intracellular cation in yeast and it has been associated with various relevant cellular processes. For this reason, potassium homeostasis is tightly controlled by several transporters, that allow the cation uptake, intracellular traffic and efflux, and by a set of proteins regulating these processes ...
... intracellular cation in yeast and it has been associated with various relevant cellular processes. For this reason, potassium homeostasis is tightly controlled by several transporters, that allow the cation uptake, intracellular traffic and efflux, and by a set of proteins regulating these processes ...
bacterial hemoglobin proteins - ETH E-Collection
... substrate oxidation to the reduction of oxygen. ...
... substrate oxidation to the reduction of oxygen. ...
Abiotic stress in plants: Late Embryogenesis Abundant proteins Imen Amara
... identified and have been classified into two groups: regulatory and functional genes (Cramer et al. 2011; Shinozaki and Yamaguchi-Shinozaki 2007) (Figure 1). The first group comprises regulatory proteins, i.e. protein factors involved in further regulation of signal transduction and stress responsiv ...
... identified and have been classified into two groups: regulatory and functional genes (Cramer et al. 2011; Shinozaki and Yamaguchi-Shinozaki 2007) (Figure 1). The first group comprises regulatory proteins, i.e. protein factors involved in further regulation of signal transduction and stress responsiv ...
THE INFLUENCE OF NUTRITIONAL PHOSPHATE DEPRIVATION ARABIDOPSIS THALIANA
... cultures by 2-dimensional gel electrophoresis. Mass spectrometry identified 18 different secreted proteins that were upregulated by at least 2-fold by –Pi Arabidopsis. They were predicted to function in Pi scavenging, cell wall and ROS metabolism, proteolysis, and pathogen responses. The relationshi ...
... cultures by 2-dimensional gel electrophoresis. Mass spectrometry identified 18 different secreted proteins that were upregulated by at least 2-fold by –Pi Arabidopsis. They were predicted to function in Pi scavenging, cell wall and ROS metabolism, proteolysis, and pathogen responses. The relationshi ...
Locked Nucleic Acid (LNA™)
... an oligonucleotide is demonstrated by an increase in the duplex melting temperature (Tm) of 2-8 ºC per LNA™ monomer (see table 1). LNA™ oligonucleotides are defined as DNA or RNA nucleotides containing one or more LNA™ nucleosides. Since the physical properties (e.g. water solubility) of these seque ...
... an oligonucleotide is demonstrated by an increase in the duplex melting temperature (Tm) of 2-8 ºC per LNA™ monomer (see table 1). LNA™ oligonucleotides are defined as DNA or RNA nucleotides containing one or more LNA™ nucleosides. Since the physical properties (e.g. water solubility) of these seque ...
Role of the non-respiratory pathways in the utilization of molecular
... Saccharomyces cerevisiae is a facultative anaerobe devoid of mitochondrial alternative oxidase. In this yeast, the structure and biogenesis of the respiratory chain, on the one hand, and the functional interactions of oxidative phosphorylation with the cellular energetic metabolism, on the other, ar ...
... Saccharomyces cerevisiae is a facultative anaerobe devoid of mitochondrial alternative oxidase. In this yeast, the structure and biogenesis of the respiratory chain, on the one hand, and the functional interactions of oxidative phosphorylation with the cellular energetic metabolism, on the other, ar ...
Metabolism and function of bile acids
... that other cytochrome P-450 enzymes, cyp3a4 in particular, partially compensate for the missing functions supplied by cyp27 in the murine species (A. Honda, 2001). Indeed, cyp27-deficient mice are still capable of producing normal C24 bile acids but overall bile acid synthesis is markedly diminished ...
... that other cytochrome P-450 enzymes, cyp3a4 in particular, partially compensate for the missing functions supplied by cyp27 in the murine species (A. Honda, 2001). Indeed, cyp27-deficient mice are still capable of producing normal C24 bile acids but overall bile acid synthesis is markedly diminished ...
endoglucanase in cellulose biosynthesis is not very clear
... Instead, the plant gene was identified by sequencing random cDNAs from a cotton fiber library and analyzing the derived amino acid sequences for presence of the conserved features identified in the bacterial cellulose synthase and other processive β-glycosyltransferases. Mutant analysis, specificall ...
... Instead, the plant gene was identified by sequencing random cDNAs from a cotton fiber library and analyzing the derived amino acid sequences for presence of the conserved features identified in the bacterial cellulose synthase and other processive β-glycosyltransferases. Mutant analysis, specificall ...
Identification and characterization of novel interaction
... SPINE analysis of StrepCcpA ......................................................................................................... 73 ...
... SPINE analysis of StrepCcpA ......................................................................................................... 73 ...
Gene Section FST (follistatin) Atlas of Genetics and Cytogenetics
... bound activin is unable to initiate signal transduction and consequently follistatin is a potent antagonist of physiological activin signals. Of the three follistatin domains present in all follistatin isoforms, (Shimasaki et al., 1988) the first two, but not the third, are necessary for activin A b ...
... bound activin is unable to initiate signal transduction and consequently follistatin is a potent antagonist of physiological activin signals. Of the three follistatin domains present in all follistatin isoforms, (Shimasaki et al., 1988) the first two, but not the third, are necessary for activin A b ...
Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the -threonine
... isolation of mutant strains of genetically defined hyper-producers. Selection criteria would include reduced by-product formation and expanded substrate spectra. By all accounts, it is by genetic engineering that breakthroughs in efficient and clean L-threonine production will be achieved. This review ...
... isolation of mutant strains of genetically defined hyper-producers. Selection criteria would include reduced by-product formation and expanded substrate spectra. By all accounts, it is by genetic engineering that breakthroughs in efficient and clean L-threonine production will be achieved. This review ...
Plant and Soil
... modification of one subunit of dinitrogenase reductase by dinitrogenase reductase ADP-ribosyltransferase ( D R A T ) . The system can be reactivated when NH~- is exhausted, by dinitrogenase reductase activating glycohydrolase ( D R A G ) which removes the inactivating group. It is fascinating that s ...
... modification of one subunit of dinitrogenase reductase by dinitrogenase reductase ADP-ribosyltransferase ( D R A T ) . The system can be reactivated when NH~- is exhausted, by dinitrogenase reductase activating glycohydrolase ( D R A G ) which removes the inactivating group. It is fascinating that s ...