The C-terminal domain of the Rhizobium leguminosarum
... Four PhoA fusions within the large hydrophilic loop were identified following the TnPhoA mutagenesis of pIJ1089 and pIJ1887. DNA sequencing of the fusion functions in plasmids pIJ1687, pIJ1924, pIJ1923 and pIJ1704 showed that the fusions were in frame and located after amino acids 17 (NodC17–PhoA), ...
... Four PhoA fusions within the large hydrophilic loop were identified following the TnPhoA mutagenesis of pIJ1089 and pIJ1887. DNA sequencing of the fusion functions in plasmids pIJ1687, pIJ1924, pIJ1923 and pIJ1704 showed that the fusions were in frame and located after amino acids 17 (NodC17–PhoA), ...
CHEMICAL SYNTHESIS OF PROTEINS : A TOOL FOR PROTEIN
... An ingenious stratagem useful to understand and modulate the structural and functional features of the proteins refers to the modification of their chemical structure. In this regard, the chemical synthesis of proteins appears a key tool, as it allows the unlimited modification of a polypeptide chai ...
... An ingenious stratagem useful to understand and modulate the structural and functional features of the proteins refers to the modification of their chemical structure. In this regard, the chemical synthesis of proteins appears a key tool, as it allows the unlimited modification of a polypeptide chai ...
Chromatin Condensing Functions of the Linker Histone C
... Intrinsically disordered protein domains mostly or completely lack classical secondary structure but undergo disorder to order transitions concomitant with binding to their macromolecular targets (9, 21, 22). While much progress has been made predicting the prevalence of intrinsically disordered pro ...
... Intrinsically disordered protein domains mostly or completely lack classical secondary structure but undergo disorder to order transitions concomitant with binding to their macromolecular targets (9, 21, 22). While much progress has been made predicting the prevalence of intrinsically disordered pro ...
Interactive exploration of genomic data from the nowac - Munin
... 2030 the authors estimated that there will be 21.3 million new cancer cases and 13.1 million deaths just because of the growth and aging of the population. Reducing the prevalence of cancer rely new tehcnologies providing new insights and understanding that lead to better treatment and more accurate ...
... 2030 the authors estimated that there will be 21.3 million new cancer cases and 13.1 million deaths just because of the growth and aging of the population. Reducing the prevalence of cancer rely new tehcnologies providing new insights and understanding that lead to better treatment and more accurate ...
Gene Section MUC4 (mucin 4, cell surface associated) in Oncology and Haematology
... pancreatic cancer. Neoexpression of MUC4 is observed early in precancerous pancreatic intraepithelial neoplastic lesions that further correlates with the disease progression stages. Many studies has been conducted on human pancreatic or other cancer cell lines in order to elucidate molecular mechani ...
... pancreatic cancer. Neoexpression of MUC4 is observed early in precancerous pancreatic intraepithelial neoplastic lesions that further correlates with the disease progression stages. Many studies has been conducted on human pancreatic or other cancer cell lines in order to elucidate molecular mechani ...
The Plant Cell
... coli resistance to a group of antimicrobial agents that are similar to but not overlapping with those to which NorM confers resistance. The significant reduction in norfloxacin accumulation in E. coli cells attributed to NorM expression can be eliminated rapidly by dissipation of the proton gradient ...
... coli resistance to a group of antimicrobial agents that are similar to but not overlapping with those to which NorM confers resistance. The significant reduction in norfloxacin accumulation in E. coli cells attributed to NorM expression can be eliminated rapidly by dissipation of the proton gradient ...
World Index of BioMolecular Visualization Resources
... Cross-indexing terms: deoxyribonucleic acid, DNA, major groove, minor groove. This Chime resource contains animations to illustrate the structure of DNA. The animations are initiated by clicking buttons on the right pannel. They show a base pair in the context of a larger helix and highlight the bas ...
... Cross-indexing terms: deoxyribonucleic acid, DNA, major groove, minor groove. This Chime resource contains animations to illustrate the structure of DNA. The animations are initiated by clicking buttons on the right pannel. They show a base pair in the context of a larger helix and highlight the bas ...
Characterization of cytochrome P450
... grown in the fields during February to August. The plants were also grown in pots and maintained in the glass house. Leaf material was collected from 5 month old plants for RNA isolation. For wound induction plants were pricked at various parts with the help of syringe and forceps. Immediately after ...
... grown in the fields during February to August. The plants were also grown in pots and maintained in the glass house. Leaf material was collected from 5 month old plants for RNA isolation. For wound induction plants were pricked at various parts with the help of syringe and forceps. Immediately after ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporter SGLT2* J. Dairy Sci. 88:2738–2748
... bSGLT2 (Figure 1). However, it is unclear whether these sites are actually glycosylated, because the in vitro translated product of bSGLT2 showed only a very minor shift in apparent molecular weight after addition of canine microsomal membranes to the reaction (see below). In addition, numerous prot ...
... bSGLT2 (Figure 1). However, it is unclear whether these sites are actually glycosylated, because the in vitro translated product of bSGLT2 showed only a very minor shift in apparent molecular weight after addition of canine microsomal membranes to the reaction (see below). In addition, numerous prot ...
Molecular analysis and biological implications of STAT3 signal
... the cytoplasm. The kinetics of STAT3 dephosphorylation and cytoplasmic re-localization occur with similar kinetics as the upregulation of SOCS proteins. SOCS proteins downregulate STAT signal transduction by association with Jaks or the activated receptor complex, thereby preventing STAT tyrosine ph ...
... the cytoplasm. The kinetics of STAT3 dephosphorylation and cytoplasmic re-localization occur with similar kinetics as the upregulation of SOCS proteins. SOCS proteins downregulate STAT signal transduction by association with Jaks or the activated receptor complex, thereby preventing STAT tyrosine ph ...
Interactions of TCA cycle enzymes and of the CcpA
... carbon sources and NADP . Excess of α-ketoglutarate can be applied to amino acid anabolism, since αketoglutarate is the precursor molecule of glutamate. No interaction was detected between Mdh and CitZ by SPR even in the presence of the corresponding metabolites. This result was surprising, because ...
... carbon sources and NADP . Excess of α-ketoglutarate can be applied to amino acid anabolism, since αketoglutarate is the precursor molecule of glutamate. No interaction was detected between Mdh and CitZ by SPR even in the presence of the corresponding metabolites. This result was surprising, because ...
In Silico Prediction of the Peroxisomal Proteome in Fungi, Plants
... decision tree to sort proteins among several different compartments. In PSORT, the PTS1 motif [AS][HKR]-L is used as a marker for peroxisomal location along with amino acid composition over the entire protein. The performance on peroxisomal proteins is modest, in the sense that many peroxisomal prot ...
... decision tree to sort proteins among several different compartments. In PSORT, the PTS1 motif [AS][HKR]-L is used as a marker for peroxisomal location along with amino acid composition over the entire protein. The performance on peroxisomal proteins is modest, in the sense that many peroxisomal prot ...
1 - University of Pennsylvania
... • Collaboration with Division of Oncology, Children’s Hospital of Philadelpia • PubMed abstracts – mining cancer literature for associations that link variations in genes with malignancies • http://bioie.ldc.upenn.edu - release 0.9 available 1157 abstracts entity annotated, 318 also treebanked ...
... • Collaboration with Division of Oncology, Children’s Hospital of Philadelpia • PubMed abstracts – mining cancer literature for associations that link variations in genes with malignancies • http://bioie.ldc.upenn.edu - release 0.9 available 1157 abstracts entity annotated, 318 also treebanked ...
The tyrosine regulated DAHP synthase and the biosynthetic
... The structural elements of the allosteric site where the feedback-inhibitor tyrosine can be bound have to communicate to the catalytic center. Amino acid residue substitutions revealed that this communication includes a signal transduction pathway within the DAHP synthase monomer. Inhibitor binding ...
... The structural elements of the allosteric site where the feedback-inhibitor tyrosine can be bound have to communicate to the catalytic center. Amino acid residue substitutions revealed that this communication includes a signal transduction pathway within the DAHP synthase monomer. Inhibitor binding ...
Engineering Acetyl Coenzyme A Supply: Functional Expression of a
... subunits presumably have no mitochondrial localization sequences; E. faecalis PDH is relatively insensitive to high NADH/ NAD⫹ ratios, which allows it to function under anaerobic as well as aerobic conditions in its natural host; the E. faecalis PDH complex has been shown to self-assemble from purif ...
... subunits presumably have no mitochondrial localization sequences; E. faecalis PDH is relatively insensitive to high NADH/ NAD⫹ ratios, which allows it to function under anaerobic as well as aerobic conditions in its natural host; the E. faecalis PDH complex has been shown to self-assemble from purif ...
Risk Assessment and Risk Management Plan
... developed by the Regulator (see Chapter 4). A risk assessment and risk management plan, including licence conditions, was then prepared to address these risks. 10. In preparing the risk assessment and risk management plan, information presented by the applicant, the scientific literature, informatio ...
... developed by the Regulator (see Chapter 4). A risk assessment and risk management plan, including licence conditions, was then prepared to address these risks. 10. In preparing the risk assessment and risk management plan, information presented by the applicant, the scientific literature, informatio ...
Structure and function of the eukaryotic ADP
... reaction converting glucose and ADP to glucose-6-phosphate and AMP. The enzyme is well studied in extremophilic archaea, where ADPGK is part of a set of glycolytic enzymes that use ADP instead of ATP for the phosphorylation of various sugars. However, ADPGK has also been found in the genomes of meso ...
... reaction converting glucose and ADP to glucose-6-phosphate and AMP. The enzyme is well studied in extremophilic archaea, where ADPGK is part of a set of glycolytic enzymes that use ADP instead of ATP for the phosphorylation of various sugars. However, ADPGK has also been found in the genomes of meso ...
A conserved N-‐terminal domain of Rif2 regulates telomere length in
... The regulation of telomere length equilibrium is essential for cell growth and survival since critically short telomeres signal DNA damage and cell cycle arrest. While the broad principles of length regulatio ...
... The regulation of telomere length equilibrium is essential for cell growth and survival since critically short telomeres signal DNA damage and cell cycle arrest. While the broad principles of length regulatio ...
PCNA Protein Expression during Spermatogenesis of the
... these forms is a 32-kDa protein with a pI value of 4.6 and the other is a 36-kDa protein with a pI of 4.7. To our knowledge, there has been no report of two forms to data of PCNA in any species. RT-PCR analysis using testis poly (A)+ RNA and primers corresponding to the 5’ and the 3’ end of the open ...
... these forms is a 32-kDa protein with a pI value of 4.6 and the other is a 36-kDa protein with a pI of 4.7. To our knowledge, there has been no report of two forms to data of PCNA in any species. RT-PCR analysis using testis poly (A)+ RNA and primers corresponding to the 5’ and the 3’ end of the open ...
Cloning and sequencing of the kedarcidin biosynthetic
... Together, the five overlapping cosmids cover 135 kb of contiguous DNA (Fig. 2A), complete DNA sequence of which led to the identification of 117 orfs (Fig. 2B). The overall GC content of the sequenced region is 73.2%, characteristic for the Actinomycetels.60 Functional assignments of genes within th ...
... Together, the five overlapping cosmids cover 135 kb of contiguous DNA (Fig. 2A), complete DNA sequence of which led to the identification of 117 orfs (Fig. 2B). The overall GC content of the sequenced region is 73.2%, characteristic for the Actinomycetels.60 Functional assignments of genes within th ...
Functional and structural roles of parasite-specific inserts in the bifunctional S-adenosylmethionine decarboxylase/ornithine
... 4.2.5.2) Dot-blot Westem analyses of SE-FPLC fractions. The collected size-exclusion chromatography fractions were transferred to nitrocellulose membranes using a BioDot apparatus (Bio-Rad) and analysed by dot blot Western. The membranes were blocked in 3% w/v low fat milk powder in lxPBS for 16 hou ...
... 4.2.5.2) Dot-blot Westem analyses of SE-FPLC fractions. The collected size-exclusion chromatography fractions were transferred to nitrocellulose membranes using a BioDot apparatus (Bio-Rad) and analysed by dot blot Western. The membranes were blocked in 3% w/v low fat milk powder in lxPBS for 16 hou ...
Mechanistic Studies Of Drug Resistance Conferred By An ABC
... the ATP-binding cassette (ABC) super-family of proteins which are found among diverse groups of organisms. In our laboratory, we are interested in studying an ABC transporter DrrAB from Streptomyces peucetius. It confers resistance to two anticancer antibiotics doxorubicin and daunorubicin by its dr ...
... the ATP-binding cassette (ABC) super-family of proteins which are found among diverse groups of organisms. In our laboratory, we are interested in studying an ABC transporter DrrAB from Streptomyces peucetius. It confers resistance to two anticancer antibiotics doxorubicin and daunorubicin by its dr ...