Predicting Protein Stability Changes upon Mutation Using Database
... core, we estimate the folding free energy changes upon mutation using database-derived potentials and correlate them with the experimentally measured ones. Several potentials are tested, representing different kinds of interactions. Local interactions along the chain are described by torsion potenti ...
... core, we estimate the folding free energy changes upon mutation using database-derived potentials and correlate them with the experimentally measured ones. Several potentials are tested, representing different kinds of interactions. Local interactions along the chain are described by torsion potenti ...
Nutrient Sensing through the Plasma Membrane of Eukaryotic Cells
... are marked for degradation in the proteasome (V. Brachet, unpublished work) by their ubiquitination, catalysed by the SCFGrr1 ubiquitin-protein ligase [19]. Proteins must be phosphorylated to be substrates of this enzyme [28], and for Mth1 and Std1 this is achieved by YckI, encoded by YCK1 and YCK2. ...
... are marked for degradation in the proteasome (V. Brachet, unpublished work) by their ubiquitination, catalysed by the SCFGrr1 ubiquitin-protein ligase [19]. Proteins must be phosphorylated to be substrates of this enzyme [28], and for Mth1 and Std1 this is achieved by YckI, encoded by YCK1 and YCK2. ...
and PITTARD
... repression of DAHP synthetase could not be directly tested. The present paper reports similar repression properties for wild-type E. coli K12. In addition, experimentation lwith aromatic auxotrophs of E. coli K12 in the chemostat reveals multivalent repression of one of the DAHP synthetases. Repress ...
... repression of DAHP synthetase could not be directly tested. The present paper reports similar repression properties for wild-type E. coli K12. In addition, experimentation lwith aromatic auxotrophs of E. coli K12 in the chemostat reveals multivalent repression of one of the DAHP synthetases. Repress ...
Instructor`s Guide
... variation besides sensitivity to PTC, including SNP association with certain diseases. In some cases SNPs may directly influence physiology (e.g. by directly altering the coding sequence for a gene), and in other cases they simply serve as biological markers that are associated with genetic traits ( ...
... variation besides sensitivity to PTC, including SNP association with certain diseases. In some cases SNPs may directly influence physiology (e.g. by directly altering the coding sequence for a gene), and in other cases they simply serve as biological markers that are associated with genetic traits ( ...
University of Groningen Interactions between carbohydrate
... Oxidizable substrates include carbohydrates, lipids and proteins. Cells are also capable of synthesizing these three types of substrates. The processes of oxidation and synthesis are ingeniously regulated. This thesis focuses on the interactions between carbohydrate and lipid metabolism, particularl ...
... Oxidizable substrates include carbohydrates, lipids and proteins. Cells are also capable of synthesizing these three types of substrates. The processes of oxidation and synthesis are ingeniously regulated. This thesis focuses on the interactions between carbohydrate and lipid metabolism, particularl ...
Research in Microbiology
... phosphorelay system, which is highly conserved in sporeforming Bacillus species (Stephenson and Hoch, 2002). In the system, histidine kinase phosphorylates Spo0F, and the phosphate group from the Spo0F is transferred to Spo0A by Spo0B. Spo0F and Spo0A are very highly conserved in the amino acid sequ ...
... phosphorelay system, which is highly conserved in sporeforming Bacillus species (Stephenson and Hoch, 2002). In the system, histidine kinase phosphorylates Spo0F, and the phosphate group from the Spo0F is transferred to Spo0A by Spo0B. Spo0F and Spo0A are very highly conserved in the amino acid sequ ...
using polymerase chain reaction for detection of enterotoxigenic
... cholera toxin, this result indicated that sequence of gene was at location 1-22 of target gene with length 564 bp of ctxA gene. Sequence of primers and length of target gene were consistent with the previous studies of Olsvik et al., (1993) and the name of this primer is ctxA’. Study specificity of ...
... cholera toxin, this result indicated that sequence of gene was at location 1-22 of target gene with length 564 bp of ctxA gene. Sequence of primers and length of target gene were consistent with the previous studies of Olsvik et al., (1993) and the name of this primer is ctxA’. Study specificity of ...
cell biology - Bio
... Cell Biology, A Short Course aims to cover a wide area of cell biology in a form especially suitable for first year undergraduates. We have deliberately kept the book to a manageable size so that neither the cost, the content, nor the weight is too daunting for the student. The overall theme for the ...
... Cell Biology, A Short Course aims to cover a wide area of cell biology in a form especially suitable for first year undergraduates. We have deliberately kept the book to a manageable size so that neither the cost, the content, nor the weight is too daunting for the student. The overall theme for the ...
ST.25 - WIPO
... modifications shall not be shown explicitly in the amino acid sequence. Any peptide or protein that can be expressed as a sequence using the symbols in Appendix 2, Table 3, in conjunction with a description elsewhere to describe, for example, abnormal linkages, cross-links (for example, disulfide br ...
... modifications shall not be shown explicitly in the amino acid sequence. Any peptide or protein that can be expressed as a sequence using the symbols in Appendix 2, Table 3, in conjunction with a description elsewhere to describe, for example, abnormal linkages, cross-links (for example, disulfide br ...
chapter 1: exploring life
... controlled experiment data deductive reasoning deoxyribonucleic acid (DNA) discovery science ecosystem emergent properties Eukarya eukaryotic cell Fungi gene genome hypothesis inductive reasoning inquiry model molecule natural selection negative feedback organ organ system organelle organism plantae ...
... controlled experiment data deductive reasoning deoxyribonucleic acid (DNA) discovery science ecosystem emergent properties Eukarya eukaryotic cell Fungi gene genome hypothesis inductive reasoning inquiry model molecule natural selection negative feedback organ organ system organelle organism plantae ...
XSL Formatter - H:\XML
... sets all contain a group of sequences that spans the same gene or region of the genome. Environmental samples are derived from a group of unclassified or unknown organisms. A population set contains sequences from different isolates of the same organism. A phylogenetic set contains sequences from di ...
... sets all contain a group of sequences that spans the same gene or region of the genome. Environmental samples are derived from a group of unclassified or unknown organisms. A population set contains sequences from different isolates of the same organism. A phylogenetic set contains sequences from di ...
Sequence and Tissue Distribution of a Second Protein of Hepatic
... map are shown in Fig. 2) revealed a single long open reading frame of 678 nucleotides, beginning at the first ATG codon encountered in Cx26-1 (278 nucleotides from the 5' end). This has the appropriate context for a eukaryotic translational initiation site with an A three nucleotides upstream and a ...
... map are shown in Fig. 2) revealed a single long open reading frame of 678 nucleotides, beginning at the first ATG codon encountered in Cx26-1 (278 nucleotides from the 5' end). This has the appropriate context for a eukaryotic translational initiation site with an A three nucleotides upstream and a ...
Goal - Cytoscape Wiki
... • Method – Calculate z-score/node, ZA score/subgraph, correct vs. random expression data sampling – Score over multiple experimental conditions – Simulated annealing used to find high scoring networks Ideker T, Ozier O, Schwikowski B, Siegel AF Bioinformatics. 2002;18 Suppl 1:S233-40 ...
... • Method – Calculate z-score/node, ZA score/subgraph, correct vs. random expression data sampling – Score over multiple experimental conditions – Simulated annealing used to find high scoring networks Ideker T, Ozier O, Schwikowski B, Siegel AF Bioinformatics. 2002;18 Suppl 1:S233-40 ...
Production of recombinant EMA-1 protein and its
... Theileria equi and Babesia caballi. This illness affects members of Equidae family (horses, mules, donkeys and zebras) and may occur in an acute, subacute or chronic form. The disease is generally characterized by fever, anemia, icterus, hepatosplenomegaly, intravascular hemolysis and petechial hemo ...
... Theileria equi and Babesia caballi. This illness affects members of Equidae family (horses, mules, donkeys and zebras) and may occur in an acute, subacute or chronic form. The disease is generally characterized by fever, anemia, icterus, hepatosplenomegaly, intravascular hemolysis and petechial hemo ...
The PRT protein family Sangita C Sinha* and Janet L Smith
... is feedback-inhibited by the end products of the pathway. Two different feedback inhibitor-binding sites overlap the Mg•PRPP site [2,9–11]. PRPP synthetase connects the nucleotide synthesis and salvage pathways, and is activated by inorganic phosphate (Pi) and inhibited by ADP. These effector molecu ...
... is feedback-inhibited by the end products of the pathway. Two different feedback inhibitor-binding sites overlap the Mg•PRPP site [2,9–11]. PRPP synthetase connects the nucleotide synthesis and salvage pathways, and is activated by inorganic phosphate (Pi) and inhibited by ADP. These effector molecu ...
Synthesis of Oligonucleotides
... chosen, since they are stable for long periods during mildly basic and acidic conditions used during oligonucleotide synthesis, and are removed with concentrated ammonia at the end of the synthesis (Section 4.1.4). The benzoyl group is used to protect both adenine and cytosine, while isobutyryl is u ...
... chosen, since they are stable for long periods during mildly basic and acidic conditions used during oligonucleotide synthesis, and are removed with concentrated ammonia at the end of the synthesis (Section 4.1.4). The benzoyl group is used to protect both adenine and cytosine, while isobutyryl is u ...
Regulators of Ubiquitin Dependent Protein Degradation in the
... substances suggesting a connection of CandA and CsnE to the regulation of secondary metabolism. This study shows that the three regulators described are crucial for fungal development, indicating that fungi are useful models to analyze the interplay between cullin containing E3 activity and differen ...
... substances suggesting a connection of CandA and CsnE to the regulation of secondary metabolism. This study shows that the three regulators described are crucial for fungal development, indicating that fungi are useful models to analyze the interplay between cullin containing E3 activity and differen ...
Chlamydia Exploit the Mammalian Tryptophan-Depletion
... distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academ ...
... distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academ ...
developing a guiding system
... of molecular biology databases. • Most of the existing molecular biology databases can be treated in this simplified manner, or as so-called flat-file databases. • Because each entry of a database is given a unique identifier, i.e., an entry name or an accession number, the molecular biology databas ...
... of molecular biology databases. • Most of the existing molecular biology databases can be treated in this simplified manner, or as so-called flat-file databases. • Because each entry of a database is given a unique identifier, i.e., an entry name or an accession number, the molecular biology databas ...
Shelef, Katie: A Critical Analysis of Degenerate Primer Design Programs
... T}. The degeneracy of primer P, d(P) = ’ li=1 ÁpiÁ, where l = length of primer P [15]. The need for degeneracy in primer design is partially due to the degeneracy of the genetic code. Because several nucleotide codons can code for the same amino acid, target DNA sequences that have differing codon u ...
... T}. The degeneracy of primer P, d(P) = ’ li=1 ÁpiÁ, where l = length of primer P [15]. The need for degeneracy in primer design is partially due to the degeneracy of the genetic code. Because several nucleotide codons can code for the same amino acid, target DNA sequences that have differing codon u ...
End-product control of enzymes of branched
... the mean in a consistent way. In all four experiments, the ratio of activities in the isoleucine-supplemented to the unsupplemented culture was above 1.5, suggesting a slight but reproducible effect of isoleucine in enhancing AHAS activity. The picture for IPMS, mediating the first specific step in ...
... the mean in a consistent way. In all four experiments, the ratio of activities in the isoleucine-supplemented to the unsupplemented culture was above 1.5, suggesting a slight but reproducible effect of isoleucine in enhancing AHAS activity. The picture for IPMS, mediating the first specific step in ...
Biochemistry and biosynthesis of insect pigments
... papiliochromes, and those sequestered from their host plants, the antioxidative carotenoids and water-soluble flavonoids. They can also be categorized into those that are produced by cyclization of linear precursors, e.g. anthraquinones, aphins and tetrapyrroles and those derived from cyclic precurs ...
... papiliochromes, and those sequestered from their host plants, the antioxidative carotenoids and water-soluble flavonoids. They can also be categorized into those that are produced by cyclization of linear precursors, e.g. anthraquinones, aphins and tetrapyrroles and those derived from cyclic precurs ...
2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... Substantial effort has been spent in pushing the resolution of optical tweezers to allow one of the most elementary events in molecular biology, the stepping of a polymerase enzyme over a single base pair, to be resolved in real time. However, what are the ultimate limits of optical tweezers and wha ...
... Substantial effort has been spent in pushing the resolution of optical tweezers to allow one of the most elementary events in molecular biology, the stepping of a polymerase enzyme over a single base pair, to be resolved in real time. However, what are the ultimate limits of optical tweezers and wha ...