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Surprising variety in energy metabolism within Trypanosomatidae
Surprising variety in energy metabolism within Trypanosomatidae

... all trypanosomatids, except for the long-slender bloodstream forms of the T. brucei family, which excrete pyruvate. In the other trypanosomatids, pyruvate is transported into the mitochondrion where it is isoxidatively decarboxylated to acetyl-CoA by the pyruvate dehydrogenase complex. Acetate is th ...
The 10.8-AA structure of Saccharomyces cerevisiae
The 10.8-AA structure of Saccharomyces cerevisiae

... largest success has been achieved for the less complex bacterial enzyme (e.g., Escherichia coli, Bacillus stearothermophilus). Bacterial phosphofructokinases are homotetramers with a total molecular weight of 140 kDa (Blangy, 1968) and their activity is mainly regulated by only two effectors, an acti ...
Microsoft Word
Microsoft Word

... Measurement of respiration and ATP synthesis/hydrolysis activities in whole mitochondria. For these assays, mitochondria were prepared by the enzymatic method of (Guerin et al., 1979). The rates of ATP synthesis were determined as described in (Rak et al., 2007a). For respiration ATP synthesis and t ...
Energy coupling in Saccharomyces cerevisiae
Energy coupling in Saccharomyces cerevisiae

... Product pathways with a positive net ATP yield provide microorganisms with free energy for growth and maintenance processes. However, during industrial production of chemicals, excess microbial biomass constitutes an undesirable byproduct, whose formation goes at the expense of the product yield. Op ...
Structure of ATP-Bound Human ATP:Cobalamin
Structure of ATP-Bound Human ATP:Cobalamin

... interfaces cover 895 and 1450 Å2) and are mediated by conserved residues, thereby indicating that the formation of the trimer is required for activity. Although the presence of differently occupied active sites could indicate allostery within the trimer, we do not favor this possibility because supe ...
How to deal with oxygen radicals stemming from mitochondrial fatty
How to deal with oxygen radicals stemming from mitochondrial fatty

... ratio between electrons entering the respiratory chain via FADH2 or NADH determines radical formation. During glucose breakdown, the ratio is low; during fatty acid breakdown, the ratio is high (the ratio increasing—asymptotically—with fatty acid length to 0.5, when compared with 0.2 for glucose). T ...
The metabolism of photosynthetically fixed
The metabolism of photosynthetically fixed

... animal, as movement of fixed carbon between the partners takes place rapidly and on a large scale. Glucose is readily metabolized by all animals, including molluscs; however, the role of glycollic acid and ;he importance of the glyoxyllate cycle in molluscan metabolism do not appear to have been inv ...
Gluconeogenesis • The biosynthesis of glucose
Gluconeogenesis • The biosynthesis of glucose

... Since we produced Glu-6-P without using an ATP, but Glu-6-P produced by hexokinase uses an ATP, we have a higher NET ATP yield. Glycolysis NET is ___ ATP (instead of the normal ___ NET ATP) (and still have 2 NADH) Glycogen phosphorylase derived Glu-6-P On your own: ...
The Enzymic Activity of the Outer Shell of
The Enzymic Activity of the Outer Shell of

... 0.1 3I-tris + maleate buffer in a 100 ml. beaker cooled in ice was disrupted by ultrasonic treatment with a 600 W. Mullard magnetostricter oscillator operating at a power level 6.9 V. at a setting of 65/lOO for 45 min. Electron microscopy showed that the cell-wall membranes after this treatment were ...
The Enzymic Activity of the Outer Shell of
The Enzymic Activity of the Outer Shell of

... 0.1 3I-tris + maleate buffer in a 100 ml. beaker cooled in ice was disrupted by ultrasonic treatment with a 600 W. Mullard magnetostricter oscillator operating at a power level 6.9 V. at a setting of 65/lOO for 45 min. Electron microscopy showed that the cell-wall membranes after this treatment were ...
The relationship of potato tubers chemical composition with selected
The relationship of potato tubers chemical composition with selected

... efficiency of photochemical reactions during the light phase, leading to produce the so-called “assimilation force”, and their relationships with enzymatic reactions during the dark phase of photosynthesis. Michałek and Sawicka (2005) as well as Loboda (2010) agree that the values of particular chlo ...
Protein Diffusion and Macromolecular Crowding in
Protein Diffusion and Macromolecular Crowding in

... C-18 for the thylakoid lipids; Duchêne and Siegenthaler, 2000). FRAP analysis of the diffusion of BODIPY FL C-12 in the PC bilayer shows that the bilayer remains fluid under these conditions, with very rapid diffusion of BODIPY FL C-12 (not shown). Thus the glass-PC system provides a fluid bilayer ...
CHAPTER 6
CHAPTER 6

... acetate is accomplished by a cycle TCA cycle, Citric Acid Cycle or Krebs Cycle • Pyruvate from glycolysis is oxidatively decarboxylated to acetate and then degraded to CO2 in TCA cycle • Some ATP is produced • More NADH and FADH2 are made (24 electrons) • NADH and FADH2 go on to make more ATP in ele ...
25-2 Carbohydrate Metabolism
25-2 Carbohydrate Metabolism

... © 2015 Pearson Education, Inc. ...
Bio1A - Lec 9 slides File
Bio1A - Lec 9 slides File

... to bump out inhibitor or to get in there before the inhibitor has a chance to bind ...
The Synthesis and Degradation of Nucleotides
The Synthesis and Degradation of Nucleotides

... (–SH) groups. Finally, the electrons are used to reduce the ribose moiety of the ribonucleotides to 2deoxyribose. During this reduction reaction the thiol (–SH) groups on Ribonucleotide Reductase are oxidized to a disulfide (–S–S–). In the second system Glutathione (a small molecule), Glutaredoxin R ...
Amplite™ Fluorimetric NADP/NADPH Assay Kit *Red
Amplite™ Fluorimetric NADP/NADPH Assay Kit *Red

... are two important cofactors found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. It forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty ...
biochem ch 20 [2-9
biochem ch 20 [2-9

... Succinate oxidized to oxaloacetate with generation of one FAD(2H) and one NADH Net reaction of TCA cycle shows 2 carbons of acetyl group oxidized to 2 CO2, with conservation of energy as 3 NADH, 1 FAD(2H), and 1 GTP  TCA cycle requires large number of vitamins and minerals to function, including ni ...
Answers - Pearson
Answers - Pearson

... Next eight electrons slightly more difficult to remove or show (relatively) small increase as these electrons are in the same energy level/ second energy level/n = 2. ...
Chapter 25: Urinary System
Chapter 25: Urinary System

... maintained outside of inner membrane • ATP synthesis occurs as H+ diffuses through a special H+ channel in inner membrane Tortora & Grabowski 9/e 2000 JWS ...
Bioenergetics, glycolysis, metabolism of monosaccharides and
Bioenergetics, glycolysis, metabolism of monosaccharides and

... 99% of monosaccharides are predominantly found in a ring (cyclic) form in solution. The aldehyde (or keto) group has reacted with an alcohol group on the same sugar, making the carbonyl carbon (carbon 1 for an aldose or carbon 2 for a ketose) asymmetric. This a symmetric carbon is referred to as ano ...
Student notes in ppt
Student notes in ppt

... Acetyl-CoA derived from fatty acid oxidation enters the Citrate Cycle only if carbohydrate metabolism is properly balanced. When fatty acid oxidation produces more acetylCoA than can be combined with OAA to form citrate, then the "extra" acetyl-CoA is converted to acetoacetyl-CoA and ketone bodies, ...
Variation in the link between oxygen consumption and ATP
Variation in the link between oxygen consumption and ATP

... nucleotide transporters (ANT), can influence the mitochondrial membrane conductance of protons and in turn the Dp [46–49]. The Dp is also likely to be affected by the active transport of cations (e.g. Ca2þ), anions (e.g. ADP32 and ATP42) and metabolites (e.g. aspartate and glutamate) across the inne ...
Chem 11 Review Answers - hrsbstaff.ednet.ns.ca
Chem 11 Review Answers - hrsbstaff.ednet.ns.ca

... b) atomic numbers c) mass numbers d) none of these 17. An element with seven electrons in the outer level would be a __. a) metal b) metalloid c) noble gas d) nonmetal 18. As the atomic number in a period increases, the degree of nonmetallic character __. a) increases c) increases then decreases b) ...
respiration in plants
respiration in plants

... The term respiration was first used by animal physiologists to describe breathing movements of animals, but was subsequently extended to include the chemical reactions by which complex organic -ve substances like carbohydrates, fats and proteins are broken down to release CO2, water and energy. In p ...
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Light-dependent reactions

In photosynthesis, the light-dependent reactions take place on the thylakoid membranes. The inside of the thylakoid membrane is called the lumen, and outside the thylakoid membrane is the stroma, where the light-independent reactions take place. The thylakoid membrane contains some integral membrane protein complexes that catalyze the light reactions. There are four major protein complexes in the thylakoid membrane: Photosystem II (PSII), Cytochrome b6f complex, Photosystem I (PSI), and ATP synthase. These four complexes work together to ultimately create the products ATP and NADPH.[.The two photosystems absorb light energy through pigments - primarily the chlorophylls, which are responsible for the green color of leaves. The light-dependent reactions begin in photosystem II. When a chlorophyll a molecule within the reaction center of PSII absorbs a photon, an electron in this molecule attains a higher energy level. Because this state of an electron is very unstable, the electron is transferred from one to another molecule creating a chain of redox reactions, called an electron transport chain (ETC). The electron flow goes from PSII to cytochrome b6f to PSI. In PSI, the electron gets the energy from another photon. The final electron acceptor is NADP. In oxygenic photosynthesis, the first electron donor is water, creating oxygen as a waste product. In anoxygenic photosynthesis various electron donors are used.Cytochrome b6f and ATP synthase work together to create ATP. This process is called photophosphorylation, which occurs in two different ways. In non-cyclic photophosphorylation, cytochrome b6f uses the energy of electrons from PSII to pump protons from the stroma to the lumen. The proton gradient across the thylakoid membrane creates a proton-motive force, used by ATP synthase to form ATP. In cyclic photophosphorylation, cytochrome b6f uses the energy of electrons from not only PSII but also PSI to create more ATP and to stop the production of NADPH. Cyclic phosphorylation is important to create ATP and maintain NADPH in the right proportion for the light-independent reactions.The net-reaction of all light-dependent reactions in oxygenic photosynthesis is:2H2O + 2NADP+ + 3ADP + 3Pi → O2 + 2NADPH + 3ATPThe two photosystems are protein complexes that absorb photons and are able to use this energy to create an electron transport chain. Photosystem I and II are very similar in structure and function. They use special proteins, called light-harvesting complexes, to absorb the photons with very high effectiveness. If a special pigment molecule in a photosynthetic reaction center absorbs a photon, an electron in this pigment attains the excited state and then is transferred to another molecule in the reaction center. This reaction, called photoinduced charge separation, is the start of the electron flow and is unique because it transforms light energy into chemical forms.
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