Name:
... Biotechnology v. DNA technology v. recombinant DNA technology Goals/uses of transformation & genetic engineering: o significance of plasmids, restriction enzymes & ligase, “sticky ends” GMOs: production, uses, controversy Animal cloning: process, controversy DNA technology o PCR o Electrop ...
... Biotechnology v. DNA technology v. recombinant DNA technology Goals/uses of transformation & genetic engineering: o significance of plasmids, restriction enzymes & ligase, “sticky ends” GMOs: production, uses, controversy Animal cloning: process, controversy DNA technology o PCR o Electrop ...
RESTRICTION ENZYMES AND VECTORS
... It is used for addition of phosphate group to an end having a free 5’- OH . ...
... It is used for addition of phosphate group to an end having a free 5’- OH . ...
GENETIC ENGINEERING QUESTIONS
... c. Perform PCR on the protein d. Grow bacteria to make the protein 2. People show restriction fragment length polymorphism because a. They have single nucleotide differences in their DNA b. The have different numbers of tandem repeats in their genes c. Both a and b d. Neither are correct 3. In gel e ...
... c. Perform PCR on the protein d. Grow bacteria to make the protein 2. People show restriction fragment length polymorphism because a. They have single nucleotide differences in their DNA b. The have different numbers of tandem repeats in their genes c. Both a and b d. Neither are correct 3. In gel e ...
Chapter 8
... DNA - Macromolecule - Small chromosomes-- Million nucleotide wide - Atcg; base pairs - Complementary; 2 strands, anti parallel - Double helix - Hydrogen bonds; at core - Dehydration synthesis - Starting point 3' prime to 5' prime - Run chemical opposite direct. - 5' phosphate - 3' hydroxyl - Deoxyri ...
... DNA - Macromolecule - Small chromosomes-- Million nucleotide wide - Atcg; base pairs - Complementary; 2 strands, anti parallel - Double helix - Hydrogen bonds; at core - Dehydration synthesis - Starting point 3' prime to 5' prime - Run chemical opposite direct. - 5' phosphate - 3' hydroxyl - Deoxyri ...
16.1 * Producing DNA Fragments
... • Many human diseases are caused by the inability of the body to produce certain protein products. • These proteins of course, are the products of a gene. • This gene may be faulty, preventing the correct expression of the gene. • There are now ways of isolating a gene, cloning it, and then transfer ...
... • Many human diseases are caused by the inability of the body to produce certain protein products. • These proteins of course, are the products of a gene. • This gene may be faulty, preventing the correct expression of the gene. • There are now ways of isolating a gene, cloning it, and then transfer ...
Ch9outline
... Nucleic Acids: DNA and RNA Structures and Properties 9.1: The basic units of DNA and RNA are nucleotides 9.2: Nucleotides form biological polymers 9.3: DNA is a double helix DNA: The Genetic Message 9.4: The nucleotide structure of DNA carries information 9.5: Specific sequences of nucleotides are g ...
... Nucleic Acids: DNA and RNA Structures and Properties 9.1: The basic units of DNA and RNA are nucleotides 9.2: Nucleotides form biological polymers 9.3: DNA is a double helix DNA: The Genetic Message 9.4: The nucleotide structure of DNA carries information 9.5: Specific sequences of nucleotides are g ...
Genetic Technology
... c. Process for Making Recombinant DNA: 1. Cleave DNA cut the desired gene (DNA sequence) using a restriction enzyme as well as the host DNA Restriction enzymes are proteins used to cut DNA between certain neulceotides on both strands of DNA There are many different restriction enzymes that ar ...
... c. Process for Making Recombinant DNA: 1. Cleave DNA cut the desired gene (DNA sequence) using a restriction enzyme as well as the host DNA Restriction enzymes are proteins used to cut DNA between certain neulceotides on both strands of DNA There are many different restriction enzymes that ar ...
Basics Terms of Life Science Cells
... The DNA molecule is arranged as a double helix, which is composed of nucleotides. Each nucleotide consists of a sugar, a phosphate and a nitrogenous base. The DNA sequence is the particular sideby-side arrangement of bases along the DNA molecule. The order of bases is important in determining the ch ...
... The DNA molecule is arranged as a double helix, which is composed of nucleotides. Each nucleotide consists of a sugar, a phosphate and a nitrogenous base. The DNA sequence is the particular sideby-side arrangement of bases along the DNA molecule. The order of bases is important in determining the ch ...
biology-final-exam-jeopardy-game
... Similar organisms that breed with each other and produce fertile offspring make up a: A. B. C. D. ...
... Similar organisms that breed with each other and produce fertile offspring make up a: A. B. C. D. ...
The Structure of DNA
... Cytosine bonds to Guanine Adenine bonds to Thymine These “bases” make the steps on a ladder The Phosphate and sugar Makes the “Backbone” ...
... Cytosine bonds to Guanine Adenine bonds to Thymine These “bases” make the steps on a ladder The Phosphate and sugar Makes the “Backbone” ...
Principles of Biology Lake Tahoe Community College
... 5. super coil. 6. DNA packing tends to prevent transcription and translation B. In female mammals, one x chromosome is inactivated in each cell 1. early in embryonic development. C. control of eukaryotic transcription 1. eukaryotes have transcription factors D. Eukaryotic RNA may be spliced in more ...
... 5. super coil. 6. DNA packing tends to prevent transcription and translation B. In female mammals, one x chromosome is inactivated in each cell 1. early in embryonic development. C. control of eukaryotic transcription 1. eukaryotes have transcription factors D. Eukaryotic RNA may be spliced in more ...
Gene Expression Vocabulary
... 21. Oncogene: a gene, that when mutated, can cause a cell to be cancerous 22. Genetic engineering: the process used to isolate a gene from the DNA of one organism and transfer the gene into the DNA of another 23. Recombinant DNA: a molecule made from pieces of DNA from separate organisms 24. Cloning ...
... 21. Oncogene: a gene, that when mutated, can cause a cell to be cancerous 22. Genetic engineering: the process used to isolate a gene from the DNA of one organism and transfer the gene into the DNA of another 23. Recombinant DNA: a molecule made from pieces of DNA from separate organisms 24. Cloning ...
Genetic Changes = Mutations
... b. THE DOG BIT THE CAT c. THE DOG BIT THE CAR (each word is representing an amino acid. The whole sentence represents a protein d. Sickle cell anemia is an example of a disease caused by this very tiny DNA error 8. Frameshift mutation: a. a single base is added or deleted in the DNA sequence b. resu ...
... b. THE DOG BIT THE CAT c. THE DOG BIT THE CAR (each word is representing an amino acid. The whole sentence represents a protein d. Sickle cell anemia is an example of a disease caused by this very tiny DNA error 8. Frameshift mutation: a. a single base is added or deleted in the DNA sequence b. resu ...
Bio Quiz #4 Review Sheet
... Structures with the same function found in animals that have a different common ancestor Caused by random events that remove genes from a population Theory that living things come from other living things Structures found in organisms with common evolutionary ancestry Adaptation in which one animal ...
... Structures with the same function found in animals that have a different common ancestor Caused by random events that remove genes from a population Theory that living things come from other living things Structures found in organisms with common evolutionary ancestry Adaptation in which one animal ...
Misconceptions relating to DNA and RNA
... A gene and the expression of the gene as a characteristic or trait are the same thing There are some types of organisms that do not have DNA Only animals have DNA, plants and mushrooms do not have DNA Each DNA molecule is made of more than one chromosome The different cell types found in a given ind ...
... A gene and the expression of the gene as a characteristic or trait are the same thing There are some types of organisms that do not have DNA Only animals have DNA, plants and mushrooms do not have DNA Each DNA molecule is made of more than one chromosome The different cell types found in a given ind ...
1 - contentextra
... might be responsible for genetic diseases, which might code for beneficial molecules that could be © Pearson Education Ltd 2010. For more information about the Pearson Baccalaureate series please visit www.pearsonbacc.com ...
... might be responsible for genetic diseases, which might code for beneficial molecules that could be © Pearson Education Ltd 2010. For more information about the Pearson Baccalaureate series please visit www.pearsonbacc.com ...
Biology Chapter 12 Review 5-6
... 7. What units make up the backbone of DNA? 8. Explain how the information Watson and Crick acquired from Rosalind Franklin and Chargaff was used to determine the structure of DNA. 9. Explain complementary base pairing and the bases involved. 10. What hold base pairs together and how many? 11. Explai ...
... 7. What units make up the backbone of DNA? 8. Explain how the information Watson and Crick acquired from Rosalind Franklin and Chargaff was used to determine the structure of DNA. 9. Explain complementary base pairing and the bases involved. 10. What hold base pairs together and how many? 11. Explai ...
DNA openbook assignment
... 2) What does DNA stand for? _____________________________ 3) State two words to describe a DNA molecule shape? ___________ __________ 4) In which organelle in the cell does the DNA exist? ____________________ 5) DNA in human cells is wound up into 23 pairs of ____________________ 6) Which of the bas ...
... 2) What does DNA stand for? _____________________________ 3) State two words to describe a DNA molecule shape? ___________ __________ 4) In which organelle in the cell does the DNA exist? ____________________ 5) DNA in human cells is wound up into 23 pairs of ____________________ 6) Which of the bas ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.