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Issues in Biotechnology
Issues in Biotechnology

... Genetically Modified somatic nuclei could be implanted into unfertilized eggs to create a genetically modified clone ...
DNA Technology
DNA Technology

... restriction enzyme is used to cut the circular DNA molecule (plasmid) found in bacteria (pink). The same enzyme is then used to cut the DNA that is required (blue). The sticky ends of each will then join up (with the help of another enzyme), inserting the required gene into the plasmid. This techniq ...
Slide 1 - Ommbid.com
Slide 1 - Ommbid.com

Chapter 15
Chapter 15

... Now that we understand genes we can change the DNA of a cell. The procedure for producing altered DNA is called ...
Chapter 12: Genetic Engineering
Chapter 12: Genetic Engineering

... Genetic engineering could not have come about without the development of a ______________________________ to support the process o A way to carefully _________________________ containing the gene away from the genes surrounding it o Find a way to ________________________________ with a piece of DNA ...
Ask A Bioloigist - Darwin and Mendel`s Afternoon Tea
Ask A Bioloigist - Darwin and Mendel`s Afternoon Tea

Study Island
Study Island

... 12. According to cell theory, cells are the basic unit of structure and function in all living things. This theory is a unifying theme in biology because A. it emphasizes the similarity of all living things. B. it points out the differences among all living things. C. it defines how living and nonli ...
12_Clicker_Questions
12_Clicker_Questions

... Restriction enzymes are useful tools for cutting DNA fragments. What do you think is the function of restriction enzymes in their normal bacterial environment? a. Restriction enzymes remove and recycle old mRNAs. b. Restriction enzymes cut up DNA taken from the environment and used as a nutrient sou ...
200 THINGS TO KNOW AP Biology TEST
200 THINGS TO KNOW AP Biology TEST

... C4 plants outcompete C3 in high sunlight because they store extra Carbon to use when CO2 conc low. CAM plants are desert succulents ( cactus) stomata close during day open at night to avoid dessication) ( C3 and C4 plants stomata opened up during day) 91. C4 uses 5ATP per molecule C3 only use 3ATP p ...
Cells - Troup County High School
Cells - Troup County High School

... DNA translation • process of translating the genetic code to the amino acid sequence • tRNA decodes the mRNA to read the DNA in order to make the correct protein ...
ERC funds Polish research into genetic material repair pathways
ERC funds Polish research into genetic material repair pathways

... not intended to have any application, but learning about DNA repair pathways is very important. When genetic material of a cells is disturbed, there are mutations, changes in genetic code. They lead to disturbance of different processes in cells and their uncontrolled growth, and thus to the formati ...
Document
Document

... The double-helix model explains Chargaff’s rule of base pairing and how the two strands of DNA are held together. The model showed the following: The two strands in the double helix run in opposite directions, with the nitrogenous bases in the center. Each strand carries a sequence of nucleotides, a ...
Document
Document

... wound around proteins (called histones) residing in the nucleus of the cell. Genes are sections of DNA that code for a specific protein that determines a particular characteristic. ...
4.1. Genetics as a Tool in Anthropology
4.1. Genetics as a Tool in Anthropology

... Statistical approach to link changes in gene structure to history of a population Gene structure can change randomly during replication or by chemical or radiation impact. The causes a change in base sequence ⇒ Mutation. Mutation can be a replacement of a base or base addition/deletion. Only a mutat ...
File - DNA Barcoding
File - DNA Barcoding

pGlo Power Point Presentation
pGlo Power Point Presentation

... RNA Polymerase araC ...
DNA Analysis in China
DNA Analysis in China

... samples. STR typing is more tolerant of degraded DNA templates than other previous methods of identification because the STR PCR products are less than 400bp long. Prior to 1993, we employed a multi-short tandem repeat (STR) system developed by our laboratory. This system included three loci: HUMTH0 ...
Final spring 2016
Final spring 2016

... 61. Inferring From which labeled structure in Figure 12–4 is structure D made? Identify that labeled structure. 62. Interpreting Graphics Identify structure F in Figure 12–4. What does it specify? 63. Interpreting Graphics What is structure E in Figure 12–4? What does it specify? 64. Predicting What ...
交通大學特色研究計畫邀請 - 國立交通大學生物資訊研究所
交通大學特色研究計畫邀請 - 國立交通大學生物資訊研究所

... These topological domains may be formed through constraining each DNA end from rotating by interacting with nuclear proteins, i.e., DNA-binding proteins. However, so far, evidence to support this hypothesis is still elusive. Here we developed two biochemical methods, i.e., DNA-nicking and DNA-gyrase ...
北京大学生命科学学院
北京大学生命科学学院

Inheritence Lecture
Inheritence Lecture

... A plasmid and the gene of interest are both cut with the same restriction endonuclease. The plasmid and gene now have complementary "sticky ends." They are incubated with DNA ligase, which reforms the two pieces as recombinant DNA. Recombinant DNA is allowed to transform a bacterial culture, which i ...
Xeroderma Pigmentosum(XP)
Xeroderma Pigmentosum(XP)

... 的) pyrimidines(嘧啶) on a DNA strand have a tendency to interact with one another to form a covalent(共价的) dimer complex.(example as TT--胸腺嘧啶二具体) ...
CBA Review
CBA Review

...  Discovery of ecosystems based on chemosynthesis in deep ...
Insertion of gene into plasmid
Insertion of gene into plasmid

... 4 Plasmid put into interest bacterial cell ...
Objective - Central Magnet School
Objective - Central Magnet School

... extraction, PCR, and restriction analysis to identify single base pair differences in DNA • Explain how single base pair changes called single nucleotide polymorphisms (SNPs) can be identified through genetic testing and often correlate to specific diseases or traits. ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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