 
									
								
									Leq: what is cloning and how is it done?
									
... 2. Reproductive cloning – generating an entire organism that is ...
                        	... 2. Reproductive cloning – generating an entire organism that is ...
									ParScore Scantrons for Lecture Tests Introduction to Microbiology Use Your Textbook Wisely
									
... ! Epulopiscium: 700 µm ! Escherichia coli: 0.5 - 2 µm ...
                        	... ! Epulopiscium: 700 µm ! Escherichia coli: 0.5 - 2 µm ...
									Bio07_TR__U04_CH13.QXD
									
... Scientists wondered whether genes from one organism would work in a different organism. Some scientists isolated the gene from fireflies that allows them to glow. Then, they inserted this gene into the DNA of a plant. These plants glowed in the dark. This showed that plants and animals use the same ...
                        	... Scientists wondered whether genes from one organism would work in a different organism. Some scientists isolated the gene from fireflies that allows them to glow. Then, they inserted this gene into the DNA of a plant. These plants glowed in the dark. This showed that plants and animals use the same ...
									DNA Similarities
									
... DNA Similarities Suppose you could compare the total DNA sequences of various organisms (some billions of base pairs). How much similarity would you expect between a whale and a fish? A whale and a dog? A dog and a shrimp? A shrimp and a bacterium? As always, there are two types of similarity to be ...
                        	... DNA Similarities Suppose you could compare the total DNA sequences of various organisms (some billions of base pairs). How much similarity would you expect between a whale and a fish? A whale and a dog? A dog and a shrimp? A shrimp and a bacterium? As always, there are two types of similarity to be ...
									Genetic engineering
									
... Alternatives to bacteria Genetically-engineered bacteria are unable to make proteins that are identical to those found naturally in humans, despite having human DNA. This is because the way in which bacteria make proteins is different to the way that mammals make proteins. A better way is to use ge ...
                        	... Alternatives to bacteria Genetically-engineered bacteria are unable to make proteins that are identical to those found naturally in humans, despite having human DNA. This is because the way in which bacteria make proteins is different to the way that mammals make proteins. A better way is to use ge ...
									Structural analysis of the protein complex involved in the
									
... its molecular mechanisms. Some eubacteria possess plasmid DNA that has acquired various antibiotic-resistant genes. The plasmid DNA is maintained in the bacteria by two types of protein, known as toxin and antitoxin. Researchers have crystallized the free YoeB toxin and the YoeB (toxin)-YefM (antito ...
                        	... its molecular mechanisms. Some eubacteria possess plasmid DNA that has acquired various antibiotic-resistant genes. The plasmid DNA is maintained in the bacteria by two types of protein, known as toxin and antitoxin. Researchers have crystallized the free YoeB toxin and the YoeB (toxin)-YefM (antito ...
									AP Biology Ch. 12 Reading Guide – Molecular Biology of the Gene
									
... AP Biology Ch. 12 Reading Guide – Molecular Biology of the Gene ...
                        	... AP Biology Ch. 12 Reading Guide – Molecular Biology of the Gene ...
									Biotechnology Guided Notes
									
...  Insulin diabetics must inject insulin because their body does not produce or use it normally  Thanks to genetic engineering, scientists have found a way to create ____________ insulin produced by _______________ or yeast Things like this are done through a process called _________________________ ...
                        	...  Insulin diabetics must inject insulin because their body does not produce or use it normally  Thanks to genetic engineering, scientists have found a way to create ____________ insulin produced by _______________ or yeast Things like this are done through a process called _________________________ ...
									Document
									
... •All cells have the same types of RNA:rRNA, tRNA, These RNAs are very much alike in sequence and structure in all cells ex:The rRNA in all organisms are greater than 50% identical in sequence and 80% in structure ...
                        	... •All cells have the same types of RNA:rRNA, tRNA, These RNAs are very much alike in sequence and structure in all cells ex:The rRNA in all organisms are greater than 50% identical in sequence and 80% in structure ...
									Human Genetics WF, ML , SFdf
									
... One strand of DNA contains many genes.  DNA is found in our blood, as blood runs throughout our whole body and can be seen as an easy way to distribute DNA  If you pulled the DNA from a single human cell, the strand would be more than one meter long! ...
                        	... One strand of DNA contains many genes.  DNA is found in our blood, as blood runs throughout our whole body and can be seen as an easy way to distribute DNA  If you pulled the DNA from a single human cell, the strand would be more than one meter long! ...
									Genetic Technology
									
... DNA fingerprinting is a kind of genetic analysis. It is famous for solving crimes. At a crime scene, police collect genetic material. Often this is blood or other bodily fluids. In a laboratory, the material is analyzed. Computers compare the DNA fingerprint against those of suspects. A match leads ...
                        	... DNA fingerprinting is a kind of genetic analysis. It is famous for solving crimes. At a crime scene, police collect genetic material. Often this is blood or other bodily fluids. In a laboratory, the material is analyzed. Computers compare the DNA fingerprint against those of suspects. A match leads ...
									Freeman 1e: How we got there
									
... Transduction • Transduction involves the transfer of host genes from one bacterium to another by bacterial viruses. ...
                        	... Transduction • Transduction involves the transfer of host genes from one bacterium to another by bacterial viruses. ...
									24 Applied genetics
									
... (a flattening of areas of the crop). Another strain (B) has a short, sturdy stem but a lower yield. The genotype of variety A is HHss (high yield, long stem) and the genotype of B is hhSS (low yield, short stem) (a) Show how a plant breeder would cross these varieties to produce a high yielding, sho ...
                        	... (a flattening of areas of the crop). Another strain (B) has a short, sturdy stem but a lower yield. The genotype of variety A is HHss (high yield, long stem) and the genotype of B is hhSS (low yield, short stem) (a) Show how a plant breeder would cross these varieties to produce a high yielding, sho ...
									NTNU brevmal
									
... B) causing specific double-strand DNA breaks that result in blunt ends on both strands C) causing linear ends of the newly replicated DNA to circularize D) adding numerous short DNA sequences such as TTAGGG E) adding numerous GC pairs which resist hydrolysis and maintain chromosome integrity 19 The ...
                        	... B) causing specific double-strand DNA breaks that result in blunt ends on both strands C) causing linear ends of the newly replicated DNA to circularize D) adding numerous short DNA sequences such as TTAGGG E) adding numerous GC pairs which resist hydrolysis and maintain chromosome integrity 19 The ...
									Molecular Genetics of Viruses
									
... • Transduction- introduction of new DNA into a bacteria by a virus – When a virus is assembled during a lytic cycle, it is sometimes assembled with some bacterial DNA in place fo some the viral DNA. – When this aberrant virus infects another cell, the bacterial DNA that it delivers can recombine wit ...
                        	... • Transduction- introduction of new DNA into a bacteria by a virus – When a virus is assembled during a lytic cycle, it is sometimes assembled with some bacterial DNA in place fo some the viral DNA. – When this aberrant virus infects another cell, the bacterial DNA that it delivers can recombine wit ...
									DNA Extraction Lab
									
... Mashed Strawberry Filtered Strawberry Strawberry with Extraction Solution Strawberry with Isopropyl Alcohol DNA ...
                        	... Mashed Strawberry Filtered Strawberry Strawberry with Extraction Solution Strawberry with Isopropyl Alcohol DNA ...
									Ch. 19 The Organization and Control of Eukaryotic Genomes
									
... diagnostic for different human populations Can be used to infer time and order of sequence duplication events ...
                        	... diagnostic for different human populations Can be used to infer time and order of sequence duplication events ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									