 
									
								
									Modern System of Bacterial Taxonomy
									
... the cell easily and react to the target ribosome in the cell (in situ)  To determine the identity, abundance and relative activity of m/o in an environment  Also to detect bacteria that have not yet been ...
                        	... the cell easily and react to the target ribosome in the cell (in situ)  To determine the identity, abundance and relative activity of m/o in an environment  Also to detect bacteria that have not yet been ...
									RESEARCH GLOSSARY
									
... Phenotype: the observable or measurable characteristics of an individual Plasmid: a heritable piece of DNA that is not part of a chromosome Polymerase chain reaction (PCR): a process that reproduces a specific stretch of DNA, going from very few copies to millions of DNA copies Polymorphism: differe ...
                        	... Phenotype: the observable or measurable characteristics of an individual Plasmid: a heritable piece of DNA that is not part of a chromosome Polymerase chain reaction (PCR): a process that reproduces a specific stretch of DNA, going from very few copies to millions of DNA copies Polymorphism: differe ...
									Old exam 2 from 2002
									
... 6. In humans, which chromosome can be found most often to vary from the normal disomic condition (i. e., monosomic, trisomic, tetrasomic)? (3 points) ...
                        	... 6. In humans, which chromosome can be found most often to vary from the normal disomic condition (i. e., monosomic, trisomic, tetrasomic)? (3 points) ...
									gene therapy - HCC Learning Web
									
... • The potential uses of cloned genes fall into two general categories. • First, the goal may be to produce a protein product. – For example, bacteria carrying the gene for human growth hormone can produce large quantities of the hormone for treating stunted growth. ...
                        	... • The potential uses of cloned genes fall into two general categories. • First, the goal may be to produce a protein product. – For example, bacteria carrying the gene for human growth hormone can produce large quantities of the hormone for treating stunted growth. ...
									page 74-81
									
... together in the helical structure by the hydrogen bonds. page 77 1 Chargaff’s data showed that the proportions of guanine and cytosine are the same in DNA, as are the proportions of adenine and thymine. This must be the case if guanine forms complementary base pairs with cytosine and if adenine pair ...
                        	... together in the helical structure by the hydrogen bonds. page 77 1 Chargaff’s data showed that the proportions of guanine and cytosine are the same in DNA, as are the proportions of adenine and thymine. This must be the case if guanine forms complementary base pairs with cytosine and if adenine pair ...
									Glossary AV 121017
									
... Identity by descent. The situation where alleles in two or more individuals are identical because of common ancestry. Identity by state. The situation where alleles in two or more individuals are identical due to coincidence or to common ancestry. kilo base pairs (1.103 bp). The tendency of DNA sequ ...
                        	... Identity by descent. The situation where alleles in two or more individuals are identical because of common ancestry. Identity by state. The situation where alleles in two or more individuals are identical due to coincidence or to common ancestry. kilo base pairs (1.103 bp). The tendency of DNA sequ ...
									GENETICS 310-PRINCIPLES OF HEREDITY
									
... MY OBJECTIVE: You will appreciate and be able to convey to others the many ways genetics impacts our daily lives. TEXT: (recommended) Human Genetics by Ricki Lewis (5th-10th) editions all OK EXTRAS: Lecture notes, study guides (learning objectives) and PDF versions of old tests with and without answ ...
                        	... MY OBJECTIVE: You will appreciate and be able to convey to others the many ways genetics impacts our daily lives. TEXT: (recommended) Human Genetics by Ricki Lewis (5th-10th) editions all OK EXTRAS: Lecture notes, study guides (learning objectives) and PDF versions of old tests with and without answ ...
									Genetics
									
... • Some mutations result in genetic disease • If the mutation is recessive then it is possible for a person to be a carrier of the disease • The frequency of mutations are increased by mutagens • Some mutagens are carcinogens ...
                        	... • Some mutations result in genetic disease • If the mutation is recessive then it is possible for a person to be a carrier of the disease • The frequency of mutations are increased by mutagens • Some mutagens are carcinogens ...
									Genetics Keywords - No Brain Too Small
									
... Any enzyme (or other protein) that is required for transcription (other than RNA polymerase). They bind to the promoter site in eukaryotes. ...
                        	... Any enzyme (or other protein) that is required for transcription (other than RNA polymerase). They bind to the promoter site in eukaryotes. ...
									rDNA = recombinant DNA Figure 1. Humulin®
									
... Step 2: Inset Gene into Plasmid Synthesized proinsulin DNA Antibiotic resistance gene ...
                        	... Step 2: Inset Gene into Plasmid Synthesized proinsulin DNA Antibiotic resistance gene ...
									Schedule
									
... • Simple DNA (plasmid) and easy to add the target DNA by using the same restriction enzymes / ligase to form recombinant DNA • Easily infect the host cells without altering the recombinant DNA thus delivering the gene of interest • Once in the human cells, the virus uses the human cells to make more ...
                        	... • Simple DNA (plasmid) and easy to add the target DNA by using the same restriction enzymes / ligase to form recombinant DNA • Easily infect the host cells without altering the recombinant DNA thus delivering the gene of interest • Once in the human cells, the virus uses the human cells to make more ...
									Whippo - cloudfront.net
									
... All vertebrates have genes that make hemoglobin Like many other genes, hemoglobin genes mutates at a fairly constant rate, even if they are in different animal groups Rate of change can be used to estimate how long ago groups or organisms diverged from one another! ...
                        	... All vertebrates have genes that make hemoglobin Like many other genes, hemoglobin genes mutates at a fairly constant rate, even if they are in different animal groups Rate of change can be used to estimate how long ago groups or organisms diverged from one another! ...
									Whippo
									
... All vertebrates have genes that make hemoglobin Like many other genes, hemoglobin genes mutates at a fairly constant rate, even if they are in different animal groups Rate of change can be used to estimate how long ago groups or organisms diverged from one another! ...
                        	... All vertebrates have genes that make hemoglobin Like many other genes, hemoglobin genes mutates at a fairly constant rate, even if they are in different animal groups Rate of change can be used to estimate how long ago groups or organisms diverged from one another! ...
									NOTES: 12.1 - History of DNA (powerpoint)
									
... ● Heat killed pathogenic bacteria had passed their disease-causing ability to the harmless strain ● Griffith called this TRANSFORMATION – One strain of bacteria (harmless) had changed into the other (harmful, or disease-causing) ● Some factor was transferred from the heat killed cells to the live ce ...
                        	... ● Heat killed pathogenic bacteria had passed their disease-causing ability to the harmless strain ● Griffith called this TRANSFORMATION – One strain of bacteria (harmless) had changed into the other (harmful, or disease-causing) ● Some factor was transferred from the heat killed cells to the live ce ...
									Chapter 20 Inheritance, Genetics, and Molecular Biology So how
									
... o Genetic analysis for personalized medicine PCR (Polymerase chain reaction) o Used to clone small pieces of DNA o Important for amplifying DNA for analysis such as in DNA fingerprinting Gene cloning o Recombinant DNA – contains DNA from 2 or more different sources that allows genes to be copies o A ...
                        	... o Genetic analysis for personalized medicine PCR (Polymerase chain reaction) o Used to clone small pieces of DNA o Important for amplifying DNA for analysis such as in DNA fingerprinting Gene cloning o Recombinant DNA – contains DNA from 2 or more different sources that allows genes to be copies o A ...
									12.1 - DNA History / Discovery
									
... ● Heat killed pathogenic bacteria had passed their disease-causing ability to the harmless strain ● Griffith called this TRANSFORMATION – One strain of bacteria (harmless) had changed into the other (harmful, or disease-causing) ● Some factor was transferred from the heat killed cells to the live ce ...
                        	... ● Heat killed pathogenic bacteria had passed their disease-causing ability to the harmless strain ● Griffith called this TRANSFORMATION – One strain of bacteria (harmless) had changed into the other (harmful, or disease-causing) ● Some factor was transferred from the heat killed cells to the live ce ...
									Chapter 9: Gene Transfer, Genetic Engineering, and Genomics
									
... Compare the events that accompany the processes of plasmid and chromosomal DNA transfer via conjugation. f. Explain the unique place of the virus in the process of bacterial transduction, and compare in detail generalized transduction and specialized transduction. g. Identify the decisive pieces of ...
                        	... Compare the events that accompany the processes of plasmid and chromosomal DNA transfer via conjugation. f. Explain the unique place of the virus in the process of bacterial transduction, and compare in detail generalized transduction and specialized transduction. g. Identify the decisive pieces of ...
									Genotyping and Copy Number Variation
									
... § PCR primers bind to target region § Allele-specific oligonucleotide probes, labeled at each end with either a fluorescent dye or a quencher molecule, bind to the variant site § Proofreading polymerase degrades the oligonucleotide probe, releasing the dye molecule resulting in a fluorescent s ...
                        	... § PCR primers bind to target region § Allele-specific oligonucleotide probes, labeled at each end with either a fluorescent dye or a quencher molecule, bind to the variant site § Proofreading polymerase degrades the oligonucleotide probe, releasing the dye molecule resulting in a fluorescent s ...
									Gene Linkage
									
... Limitations of selective breeding and mutations: – Selective breeding requires traits already exists in a population – we can not make new traits. – Mutations are unpredictable and will not create the exact traits that we want. (most mutations are harmful to the organism) Scientists are learning how ...
                        	... Limitations of selective breeding and mutations: – Selective breeding requires traits already exists in a population – we can not make new traits. – Mutations are unpredictable and will not create the exact traits that we want. (most mutations are harmful to the organism) Scientists are learning how ...
									2 Exam paper_2006[1] - University of Leicester
									
... 1. Describe a strategy that could be employed to produce a representative genomic library that could be screened to identify a functional bacterial gene. Highlight any features that would be useful in the cloning vector used to make the library. 2. Why is DNA replication semi-discontinuous? ...
                        	... 1. Describe a strategy that could be employed to produce a representative genomic library that could be screened to identify a functional bacterial gene. Highlight any features that would be useful in the cloning vector used to make the library. 2. Why is DNA replication semi-discontinuous? ...
									Identification of animal tissue in support of WIIS
									
... Entire animals are normally easy to identify, although identification of juveniles can sometimes be problematic. However, often when we are investigating wildlife crime the whole animal is not available. This may be due to predation or decomposition of carcasses or it may be that the only way to ide ...
                        	... Entire animals are normally easy to identify, although identification of juveniles can sometimes be problematic. However, often when we are investigating wildlife crime the whole animal is not available. This may be due to predation or decomposition of carcasses or it may be that the only way to ide ...
									revolution in evolution
									
... • Discovery that DNA is the molecular material of genes, cracking genetic code • Molecular mechanisms worked out for DNA replication and protein synthesis • Multiple methods invented to study genetic variation and evolution ...
                        	... • Discovery that DNA is the molecular material of genes, cracking genetic code • Molecular mechanisms worked out for DNA replication and protein synthesis • Multiple methods invented to study genetic variation and evolution ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
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