RESEARCH ARTICLES
... Fig. 2. Analysis of the assembly intermediates. (A) Not I and Sbf I double restriction digestion analysis of assembly 341350 purified from E. coli. These restriction enzymes release the vector fragments (5.5 and 3.4 kb) from the 10-kb insert. Insert DNA was separated from the vector DNA on a 0.8% E ...
... Fig. 2. Analysis of the assembly intermediates. (A) Not I and Sbf I double restriction digestion analysis of assembly 341350 purified from E. coli. These restriction enzymes release the vector fragments (5.5 and 3.4 kb) from the 10-kb insert. Insert DNA was separated from the vector DNA on a 0.8% E ...
Purification to homogeneity and partial amino acid sequence of a
... to purification to homogeneity of C'-MT was the detection of different forms of the [3H-CH3]methylated, inactivated enzyme in partially purified human spleen extract, which included forms larger than the single ~24kDa enzyme usually observed in similarly prepared and methylated extracts of mammalian ...
... to purification to homogeneity of C'-MT was the detection of different forms of the [3H-CH3]methylated, inactivated enzyme in partially purified human spleen extract, which included forms larger than the single ~24kDa enzyme usually observed in similarly prepared and methylated extracts of mammalian ...
Development of an internally controlled real-time PCR
... In general, serological tests for the diagnosis of C. psittaci infection are hampered by lack of sensitivity and specificity (genus and ⁄ or species) and poor reproducibility [7,23–25]. The two PCRpositive, but serologically negative, cases in the present study highlight this problem. A PCR false-po ...
... In general, serological tests for the diagnosis of C. psittaci infection are hampered by lack of sensitivity and specificity (genus and ⁄ or species) and poor reproducibility [7,23–25]. The two PCRpositive, but serologically negative, cases in the present study highlight this problem. A PCR false-po ...
The promiscuous primase
... range of eukaryotic DNA Pol Xs and the small subunit of archaeal and eukaryotic primases [15]. With the elucidation of the structure of human DNA Pol b and the first crystal structure of an archaeal primase, it became apparent that the primary sequence similarity was mirrored by a degree of structur ...
... range of eukaryotic DNA Pol Xs and the small subunit of archaeal and eukaryotic primases [15]. With the elucidation of the structure of human DNA Pol b and the first crystal structure of an archaeal primase, it became apparent that the primary sequence similarity was mirrored by a degree of structur ...
Electron transfer from aromatic amino acids to guanine and adenine
... away from the site of its initial formation and then initiate a DNA lesion. In living cells, this ability of DNA can be employed for redox sensing and signaling in the genome.17 Several experiments in vitro have demonstrated that radical cation states in DNA are transmitted over distances up to ~200 ...
... away from the site of its initial formation and then initiate a DNA lesion. In living cells, this ability of DNA can be employed for redox sensing and signaling in the genome.17 Several experiments in vitro have demonstrated that radical cation states in DNA are transmitted over distances up to ~200 ...
7. molecular genetics.
... 6. BIOTECHNOLOGY. •CREATING GENETICALLY MODIFIED ORGANISMS. These plants or animals have traits which the original plant or animal did not have because they have been given genes from another organism. ...
... 6. BIOTECHNOLOGY. •CREATING GENETICALLY MODIFIED ORGANISMS. These plants or animals have traits which the original plant or animal did not have because they have been given genes from another organism. ...
Comprehensive Analysis of Hyrdrogen Bonds in Regulatory Protein
... DNA backbone. Interactions that involve atoms in the protein backbone were found predominantly with the DNA backbone rather than with the base edges. The scarcity of Pbb × Dbe contacts (nine only) is probably due to conformational restrictions that prevent their formation. Most of the interactions w ...
... DNA backbone. Interactions that involve atoms in the protein backbone were found predominantly with the DNA backbone rather than with the base edges. The scarcity of Pbb × Dbe contacts (nine only) is probably due to conformational restrictions that prevent their formation. Most of the interactions w ...
Bioinformatics - Sequences and Computers
... Learn how information-bearing sequences are different from random sequences and become familiar with bioinformatics tools for the analysis of sequences. Language and DNA use sequences to communicate information. The sequence elements in language are letters and punctuation, in DNA they are the nucle ...
... Learn how information-bearing sequences are different from random sequences and become familiar with bioinformatics tools for the analysis of sequences. Language and DNA use sequences to communicate information. The sequence elements in language are letters and punctuation, in DNA they are the nucle ...
MI Semester Two Study Guide - Kenwood Academy High School
... What is a zone of inhibition test? What does the test show? How can you tell if a bacterium is antibiotic resistant? (1 MC) Diagnose a patient with the appropriate type of hearing loss and suggest a medication intervention. (1 MC) What is attack rate? How is it calculated? What conclusions can be ma ...
... What is a zone of inhibition test? What does the test show? How can you tell if a bacterium is antibiotic resistant? (1 MC) Diagnose a patient with the appropriate type of hearing loss and suggest a medication intervention. (1 MC) What is attack rate? How is it calculated? What conclusions can be ma ...
Plasma Nucleic Acids in the Diagnosis and Management
... Frickhofen et al. (32 ) demonstrated the presence of rearranged immunoglobulin gene sequences in the plasma and serum of patients with B-cell malignancies. For follicular lymphomas, characterized by a chromosome 14;18 translocation that juxtaposes the BCL2 oncogene with the immunoglobulin heavy chai ...
... Frickhofen et al. (32 ) demonstrated the presence of rearranged immunoglobulin gene sequences in the plasma and serum of patients with B-cell malignancies. For follicular lymphomas, characterized by a chromosome 14;18 translocation that juxtaposes the BCL2 oncogene with the immunoglobulin heavy chai ...
Chapter 17
... encodes needed to build/maintain organisms can be passed to the next generation. 2. …stores this information that will be used to make all the RNA/polypeptides that will directly build/maintain the organism. ...
... encodes needed to build/maintain organisms can be passed to the next generation. 2. …stores this information that will be used to make all the RNA/polypeptides that will directly build/maintain the organism. ...
Tissue Engineering for In Vitro Analysis of Matrix Metalloproteinases
... Effect of Decorin on Keloid Fibroblasts in the Matrigel ModelLetters indicate statistical significance from HS27 at corresponding time point (P < .01b); plus signs, statistical significance from day 7 of same cell type (P < .001a). A, DNA quantification normalized to respective dry weights indicated ...
... Effect of Decorin on Keloid Fibroblasts in the Matrigel ModelLetters indicate statistical significance from HS27 at corresponding time point (P < .01b); plus signs, statistical significance from day 7 of same cell type (P < .001a). A, DNA quantification normalized to respective dry weights indicated ...
Probing b-Lactamase Structure and Function Using Random Replacement Mutagenesis.
... residue positions are actually important for the structure and function of the protein as well as where those important regions lie. Detailed information about the sequence requirements of individual residues is obtained by sequencing functional substitutions. The amino acid side chain characteristi ...
... residue positions are actually important for the structure and function of the protein as well as where those important regions lie. Detailed information about the sequence requirements of individual residues is obtained by sequencing functional substitutions. The amino acid side chain characteristi ...
An Introduction to Genetic Analysis Chapter 20 Transposable
... In 1938, Marcus Rhoades analyzed an ear of Mexican black corn. The ear came from a selfing of a pure-breeding pigmented genotype, but it showed a surprising modified Mendelian dihybrid segregation ratio of 12:3:1 among pigmented, dotted, and colorless kernels. Analysis showed that two events had occ ...
... In 1938, Marcus Rhoades analyzed an ear of Mexican black corn. The ear came from a selfing of a pure-breeding pigmented genotype, but it showed a surprising modified Mendelian dihybrid segregation ratio of 12:3:1 among pigmented, dotted, and colorless kernels. Analysis showed that two events had occ ...
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... from the euphotic zone at Station 2 and the chlorophyll maximum at Station 3 (Table 1) were sequenced, no nifH DNA sequences were detected among those clones. This suggests that N2-fixing cyanobacteria were not abundant in these waters. Nevertheless, we did detect nifH gene expression: four cDNA seq ...
... from the euphotic zone at Station 2 and the chlorophyll maximum at Station 3 (Table 1) were sequenced, no nifH DNA sequences were detected among those clones. This suggests that N2-fixing cyanobacteria were not abundant in these waters. Nevertheless, we did detect nifH gene expression: four cDNA seq ...
DNA How the Molecule of Heredity Carries, Replicates, and
... Pol I – fills in gaps between newly synthesized Okazaki segments DNA helicase – unwinds double helix Single-stranded binding proteins – keep helix open Primase – creates RNA primers to initiate synthesis Ligase – welds together Okazaki fragments Copyright © The McGraw-Hill Companies, Inc. Permission ...
... Pol I – fills in gaps between newly synthesized Okazaki segments DNA helicase – unwinds double helix Single-stranded binding proteins – keep helix open Primase – creates RNA primers to initiate synthesis Ligase – welds together Okazaki fragments Copyright © The McGraw-Hill Companies, Inc. Permission ...
Discovery of MLL1 binding units, their localization to CpG Islands
... Background: Mixed Lineage Leukemia 1 (MLL1) is a mammalian ortholog of the Drosophila Trithorax. In Drosophila, Trithorax complexes transmit the memory of active genes to daughter cells through interactions with Trithorax Response Elements (TREs). However, despite their functional importance, nothin ...
... Background: Mixed Lineage Leukemia 1 (MLL1) is a mammalian ortholog of the Drosophila Trithorax. In Drosophila, Trithorax complexes transmit the memory of active genes to daughter cells through interactions with Trithorax Response Elements (TREs). However, despite their functional importance, nothin ...
infection
... Subject: Pulsed-field gel electrophoresis results Jenna, The important thing for you to look at in the figure I’ve attached is the pattern of bands. These are bands of DNA; each band represents a piece of DNA of a specific length (see my note in the P.S.). Compare the pattern obtained from your pati ...
... Subject: Pulsed-field gel electrophoresis results Jenna, The important thing for you to look at in the figure I’ve attached is the pattern of bands. These are bands of DNA; each band represents a piece of DNA of a specific length (see my note in the P.S.). Compare the pattern obtained from your pati ...
Microbial Diversity in Prince Edward County`s Soil Microbiome
... thousands of types of microbial bacteria that inhabit many different habitats and environments. New types of microbial bacteria are often discovered, so there is a lot about it that scientists are unsure of. Microbial bacteria play an important role in the ecosystems they inhabit, as well as indicat ...
... thousands of types of microbial bacteria that inhabit many different habitats and environments. New types of microbial bacteria are often discovered, so there is a lot about it that scientists are unsure of. Microbial bacteria play an important role in the ecosystems they inhabit, as well as indicat ...
Proposed alignment of helical interruptions in the two subunits of the
... and 8, Ala is substituted for Gly. Either one of these substitutions will not affect the physical parameters of the collagen molecule substantially (Mao, B. and Vogeli, G., unpublished data from computer modelling). It is, however, also possible that the cyz (IV) chain was the ancestral gene with ma ...
... and 8, Ala is substituted for Gly. Either one of these substitutions will not affect the physical parameters of the collagen molecule substantially (Mao, B. and Vogeli, G., unpublished data from computer modelling). It is, however, also possible that the cyz (IV) chain was the ancestral gene with ma ...
Powerpoint Presentation
... •The concentration of X-gal is constant at 0.5M within the cell at all times. •The concentration of X-gal is sufficiently large to have the rate of change of indigo be equal to Vmax. •Vmax is equal to Kcat multiplied by the concentration of β-galactosidase. •The concentration of β-galactosidase is e ...
... •The concentration of X-gal is constant at 0.5M within the cell at all times. •The concentration of X-gal is sufficiently large to have the rate of change of indigo be equal to Vmax. •Vmax is equal to Kcat multiplied by the concentration of β-galactosidase. •The concentration of β-galactosidase is e ...
IGEM_presentation
... ai is rate of production of i in absence of repression, bi is the binding constant of component I to its activator, ei is the inverse rate of breakdown of component i. L relates to LexA, R to RecA, R* to RecA* and S to ssDNA Ki is the binding constant of component i to the LexA gene. Cs is the conce ...
... ai is rate of production of i in absence of repression, bi is the binding constant of component I to its activator, ei is the inverse rate of breakdown of component i. L relates to LexA, R to RecA, R* to RecA* and S to ssDNA Ki is the binding constant of component i to the LexA gene. Cs is the conce ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.