Sequence, expression, and characterization of the first archaeal ATP
... and eukaryal species. A general feature of most ATPPFKs from the domains of Bacteria and Eukarya is their homotetrameric stucture and the allosteric regulation of activity by compounds of intermediary metabolism. The bacterial enzymes are usually composed of 34-kDa subunits, allosterically activated ...
... and eukaryal species. A general feature of most ATPPFKs from the domains of Bacteria and Eukarya is their homotetrameric stucture and the allosteric regulation of activity by compounds of intermediary metabolism. The bacterial enzymes are usually composed of 34-kDa subunits, allosterically activated ...
A series of vectors for fungal transformation
... modified polylinker in pBluescript II and pBC (pCB1519 and pCB1520, respectively) where the XhoI site is flanked on both sides by SmaI sites. Second, the selectable markers were cloned into common cloning vectors outside the polylinker, thus leaving the lacZ gene intact. Most of the restriction enzy ...
... modified polylinker in pBluescript II and pBC (pCB1519 and pCB1520, respectively) where the XhoI site is flanked on both sides by SmaI sites. Second, the selectable markers were cloned into common cloning vectors outside the polylinker, thus leaving the lacZ gene intact. Most of the restriction enzy ...
An assessment of the risks associated with the
... the plant cell where the natural integration properties of the T-DNA insert the entire gene ensemble into the chromosomal DNA.6,9–11 Engineered plant DNA can also be delivered into plant cells by particle bombardment (biolistic transformation), when the DNA is literally shot into plant cells on DNA- ...
... the plant cell where the natural integration properties of the T-DNA insert the entire gene ensemble into the chromosomal DNA.6,9–11 Engineered plant DNA can also be delivered into plant cells by particle bombardment (biolistic transformation), when the DNA is literally shot into plant cells on DNA- ...
Next generation sequencing
... the cost per basepair sequence down by orders of magnitude relative to the previous standard method (Sanger sequencing with four color dye terminators, thermal cycling, and capillary electrophoresis). Massively parallel sequencing generates 100 Mb to 1 Gb of short sequence reads in a single experime ...
... the cost per basepair sequence down by orders of magnitude relative to the previous standard method (Sanger sequencing with four color dye terminators, thermal cycling, and capillary electrophoresis). Massively parallel sequencing generates 100 Mb to 1 Gb of short sequence reads in a single experime ...
Revision PowerPoint B2 Topic 1
... FARMING: Weed growth forces crops to compete for sunlight and nutrients, often leading to losses. Because herbicides cannot differentiate between plants that are crops and plants that are weeds, farmers use 'selective' herbicides. Such herbicides do not harm the crop, but are not effective at r ...
... FARMING: Weed growth forces crops to compete for sunlight and nutrients, often leading to losses. Because herbicides cannot differentiate between plants that are crops and plants that are weeds, farmers use 'selective' herbicides. Such herbicides do not harm the crop, but are not effective at r ...
Assembly of complete KIR haplotypes from a diploid individual
... unambiguously assemble individual haplotypes for the highly repetitive 100-200 kb killer Ig-like receptor (KIR) gene loci of chromosome 19. A tiling of targeted fosmids can be used to clone extended lengths of genomic DNA, 100s of kb in length, but repeat complexity in regions of particular interest ...
... unambiguously assemble individual haplotypes for the highly repetitive 100-200 kb killer Ig-like receptor (KIR) gene loci of chromosome 19. A tiling of targeted fosmids can be used to clone extended lengths of genomic DNA, 100s of kb in length, but repeat complexity in regions of particular interest ...
S - www2
... Using the Preparative Ultracentrifuge to obtain an S-value Most biochemical laboratories are equipped with a preparative ultracentrifuge. These instruments do not have optics needed to measure the distribution of protein or any other substance in a tube. Instead, if one wants to determine the distr ...
... Using the Preparative Ultracentrifuge to obtain an S-value Most biochemical laboratories are equipped with a preparative ultracentrifuge. These instruments do not have optics needed to measure the distribution of protein or any other substance in a tube. Instead, if one wants to determine the distr ...
The Close Relationship Between the A and B Genomes in Avena L
... Aena ailoiana (Malz.) Mordv. strongly and uniformly, revealing the close relationship between these two genomes. Comparison of patterns of size-separated DNA restriction fragments between the diploid A. strigosa and the tetraploid A. ailoiana, using 32 different restriction enzymes, revealed ...
... Aena ailoiana (Malz.) Mordv. strongly and uniformly, revealing the close relationship between these two genomes. Comparison of patterns of size-separated DNA restriction fragments between the diploid A. strigosa and the tetraploid A. ailoiana, using 32 different restriction enzymes, revealed ...
the Acetyl-Coenzyme A
... plasmid with XbaI and PstI and transformed into strain 144-3A. The resulting gene disruption mutant was called MM67 (a ura3, leu2, his4, hurl, acsl:: LEU2). Gene disruption was confirmed by Southern analysis (Southern. 1975; Sambrook et a/., 1989) of genomic DNA digested with either BamHI or EcoRI. ...
... plasmid with XbaI and PstI and transformed into strain 144-3A. The resulting gene disruption mutant was called MM67 (a ura3, leu2, his4, hurl, acsl:: LEU2). Gene disruption was confirmed by Southern analysis (Southern. 1975; Sambrook et a/., 1989) of genomic DNA digested with either BamHI or EcoRI. ...
De Bruijn Graphs for DNA Sequencing (Part 1)
... an alternative sequencing method. Nobody believed it will ever work • 1991: Light directed polymer synthesis developed by Steve Fodor and colleagues. ...
... an alternative sequencing method. Nobody believed it will ever work • 1991: Light directed polymer synthesis developed by Steve Fodor and colleagues. ...
Damage Control: The Pleiotropy of DNA Repair Genes
... cells. This result has been confirmed and extended to cells in the eye imaginal disc (M. Brodsky and G. M. Rubin, personal communication). Thus the function of the MEI-41 protein may not be in the repair of damage per se, but in triggering a DNA damage-dependent cellcycle checkpoint. Activation of t ...
... cells. This result has been confirmed and extended to cells in the eye imaginal disc (M. Brodsky and G. M. Rubin, personal communication). Thus the function of the MEI-41 protein may not be in the repair of damage per se, but in triggering a DNA damage-dependent cellcycle checkpoint. Activation of t ...
Genetics revisited - Institut Montefiore
... These were originally discovered in 1868 by Friedrich Meischer (isolating DNA from pus cells on bandages). At that time, he could not confirm that nucleic acids might contain genetic information. DNA IS the genetic information of most living organisms. In contrast, some viruses (called retroviruse ...
... These were originally discovered in 1868 by Friedrich Meischer (isolating DNA from pus cells on bandages). At that time, he could not confirm that nucleic acids might contain genetic information. DNA IS the genetic information of most living organisms. In contrast, some viruses (called retroviruse ...
an integrated microsystem for allele
... of the anticoagulant drug Warfarin [1]. However, medical care providers are often not equipped to perform SNP genotyping on-site and must ship samples to a specialized lab, which adds cost and time. There is a clear market desire for a point-of-care, microfluidic-based system capable of fast and aff ...
... of the anticoagulant drug Warfarin [1]. However, medical care providers are often not equipped to perform SNP genotyping on-site and must ship samples to a specialized lab, which adds cost and time. There is a clear market desire for a point-of-care, microfluidic-based system capable of fast and aff ...
Answers - Study of Life
... Insulin injected by diabetics to control blood sugar levels is derived from bacteria whose DNA has been modified by the addition of the human gene for insulin, which is then produced by the prokaryotes. This is an example of: A. acid therapy B. cloning C. genetic engineering D. gene therapy E. pluri ...
... Insulin injected by diabetics to control blood sugar levels is derived from bacteria whose DNA has been modified by the addition of the human gene for insulin, which is then produced by the prokaryotes. This is an example of: A. acid therapy B. cloning C. genetic engineering D. gene therapy E. pluri ...
Central Dogma of Molecular Biology Chapter 28 DNA Replication
... RNA polymerase binds to promoter sites on the DNA template to initiate transcription The elongation process is common to all organisms. In contrast, the processes of initiation and termination differ substantially in bacteria and eukaryotes. We begin with a discussion of these processes in bacteria ...
... RNA polymerase binds to promoter sites on the DNA template to initiate transcription The elongation process is common to all organisms. In contrast, the processes of initiation and termination differ substantially in bacteria and eukaryotes. We begin with a discussion of these processes in bacteria ...
KOD -Plus- Mutagenesis Kit
... 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. ...
... 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. ...
Applied Environmnetal Microbiology
... into pKK233-2 digested with HindIII after cohesive ends were filled in with the Klenow fragment, generating pBC73. The cryV465 gene was also cloned into pKK233-2 by the same procedure to make pBC83. E. coli JM109 harboring pBC73 or pBC83 expressed the toxin protein constitutively without the additio ...
... into pKK233-2 digested with HindIII after cohesive ends were filled in with the Klenow fragment, generating pBC73. The cryV465 gene was also cloned into pKK233-2 by the same procedure to make pBC83. E. coli JM109 harboring pBC73 or pBC83 expressed the toxin protein constitutively without the additio ...
1 PERKINELMER™ LIFE SCIENCES, INC. OLIGONUCLEOTIDE 5
... information of the target DNA or RNA in several hours on a DNA synthesizer. This eliminates the usual cumbersome and time consuming steps that are involved in the cloning and isolation of restriction fragments to be used as hybridization probes. Another advantage of oligonucleotide probes is that th ...
... information of the target DNA or RNA in several hours on a DNA synthesizer. This eliminates the usual cumbersome and time consuming steps that are involved in the cloning and isolation of restriction fragments to be used as hybridization probes. Another advantage of oligonucleotide probes is that th ...
Water, Air, and Soil Pollution: Focus
... herbicide in many crops). It is also used to spray the crop just before harvesting in order to speed up maturing of the seeds and facilitate harvest. The gene for Roundup Ready® or glyphosate tolerance was derived from Agrobacterium sp. strain CP4. The gene encodes for a protein, CP4-EPSPS that is n ...
... herbicide in many crops). It is also used to spray the crop just before harvesting in order to speed up maturing of the seeds and facilitate harvest. The gene for Roundup Ready® or glyphosate tolerance was derived from Agrobacterium sp. strain CP4. The gene encodes for a protein, CP4-EPSPS that is n ...
The whole paper can be downloaded here if you like.
... to mix 6 μl of DNA with 2 μl of loading dye. Then the mixture was centrifuged and pipetted into an electrophoresis gel that was prepared by the LBS 145 lab TA’s. The DNA remaining was stored at –20 ˚C. The third and final portion in the process of identifying the unknown plasmid was DNA fingerprinti ...
... to mix 6 μl of DNA with 2 μl of loading dye. Then the mixture was centrifuged and pipetted into an electrophoresis gel that was prepared by the LBS 145 lab TA’s. The DNA remaining was stored at –20 ˚C. The third and final portion in the process of identifying the unknown plasmid was DNA fingerprinti ...
Genetic mapping of Theobroma cacao (Malvaceae - Funpec-RP
... genitors, only five of the 35 primers (Lanaud et al., 1999; Risterucci et al., 2000) (mTcCIR 6, mTcCIR 8, mTcCIR 12, mTcCIR 13, and mTcCIR 60) were monomorphic for both genotypes (Pa 30 and Pa 169); 20 were polymorphic. The remaining 10 primers (mTcCIR 1, mTcCIR 7, mTcCIR 11, mTcCIR 17, mTcCIR 22, m ...
... genitors, only five of the 35 primers (Lanaud et al., 1999; Risterucci et al., 2000) (mTcCIR 6, mTcCIR 8, mTcCIR 12, mTcCIR 13, and mTcCIR 60) were monomorphic for both genotypes (Pa 30 and Pa 169); 20 were polymorphic. The remaining 10 primers (mTcCIR 1, mTcCIR 7, mTcCIR 11, mTcCIR 17, mTcCIR 22, m ...
MD Simulations of the P53 oncoprotein structure
... in p53c, experiments point to the importance of zinc coordination for achieving the correct folding and correct binding of p53 to a specific DNA in intact cells. In our simulation, however, both non-bonded and bonded approaches are used to describe the zinc-p53c binding interface. It should be stres ...
... in p53c, experiments point to the importance of zinc coordination for achieving the correct folding and correct binding of p53 to a specific DNA in intact cells. In our simulation, however, both non-bonded and bonded approaches are used to describe the zinc-p53c binding interface. It should be stres ...
3 - Dr. Jerry Cronin
... 2 Once attached to the ER, the SRP is released and the growing polypeptide snakes through the ER membrane pore into the cisterna. 3 The signal sequence is clipped off by an enzyme. As protein synthesis continues, sugar groups may be added to the protein. ...
... 2 Once attached to the ER, the SRP is released and the growing polypeptide snakes through the ER membrane pore into the cisterna. 3 The signal sequence is clipped off by an enzyme. As protein synthesis continues, sugar groups may be added to the protein. ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.