Genetic mapping of Theobroma cacao (Malvaceae - Funpec-RP
... genitors, only five of the 35 primers (Lanaud et al., 1999; Risterucci et al., 2000) (mTcCIR 6, mTcCIR 8, mTcCIR 12, mTcCIR 13, and mTcCIR 60) were monomorphic for both genotypes (Pa 30 and Pa 169); 20 were polymorphic. The remaining 10 primers (mTcCIR 1, mTcCIR 7, mTcCIR 11, mTcCIR 17, mTcCIR 22, m ...
... genitors, only five of the 35 primers (Lanaud et al., 1999; Risterucci et al., 2000) (mTcCIR 6, mTcCIR 8, mTcCIR 12, mTcCIR 13, and mTcCIR 60) were monomorphic for both genotypes (Pa 30 and Pa 169); 20 were polymorphic. The remaining 10 primers (mTcCIR 1, mTcCIR 7, mTcCIR 11, mTcCIR 17, mTcCIR 22, m ...
MD Simulations of the P53 oncoprotein structure
... in p53c, experiments point to the importance of zinc coordination for achieving the correct folding and correct binding of p53 to a specific DNA in intact cells. In our simulation, however, both non-bonded and bonded approaches are used to describe the zinc-p53c binding interface. It should be stres ...
... in p53c, experiments point to the importance of zinc coordination for achieving the correct folding and correct binding of p53 to a specific DNA in intact cells. In our simulation, however, both non-bonded and bonded approaches are used to describe the zinc-p53c binding interface. It should be stres ...
Cells Part C PPT
... 2 Once attached to the ER, the SRP is released and the growing polypeptide snakes through the ER membrane pore into the cisterna. 3 The signal sequence is clipped off by an enzyme. As protein synthesis continues, sugar groups may be added to the protein. ...
... 2 Once attached to the ER, the SRP is released and the growing polypeptide snakes through the ER membrane pore into the cisterna. 3 The signal sequence is clipped off by an enzyme. As protein synthesis continues, sugar groups may be added to the protein. ...
Identification of Human Polymorphisms in the Phenylthio
... The success of the PCR reactions will be determined by gel electrophoresis on Day 2 of the project, and DNA from successful PCR reactions will be purified and prepared for shipping to the Biotechnology Resource Center at Cornell University for sequencing on Day 3. Investigators will then use NCBI’ ...
... The success of the PCR reactions will be determined by gel electrophoresis on Day 2 of the project, and DNA from successful PCR reactions will be purified and prepared for shipping to the Biotechnology Resource Center at Cornell University for sequencing on Day 3. Investigators will then use NCBI’ ...
Cloning and characterisation of a cysteine proteinase gene
... nucleotide 1,248 –1,262 (50 -CCC GAA TTC GGC CGT TGT CGT CGG-30 ) contained a Bam HI and Eco RI restriction site sequences, respectively, for directional cloning. For PCR reaction we used 200 ng of L. (L.) amazonensis genomic DNA, 100 pmol of each primer, 2 mM dNTP mix and 3 mM MgCl2 in a volume of ...
... nucleotide 1,248 –1,262 (50 -CCC GAA TTC GGC CGT TGT CGT CGG-30 ) contained a Bam HI and Eco RI restriction site sequences, respectively, for directional cloning. For PCR reaction we used 200 ng of L. (L.) amazonensis genomic DNA, 100 pmol of each primer, 2 mM dNTP mix and 3 mM MgCl2 in a volume of ...
Geminivirus Replication Origins Have a Modular
... by geminiviruses with bipartite genomes (Howarth and Vandemark, 1989; Etessami et al., 1991; Lazarowitz et al., 1992). This conservation also extends to the AL1 homologs encoded by geminiviruses with a single genome component (Mullineaux et al., 1985; Accotto et al., 1989; Lazarowitz et al., 1989; S ...
... by geminiviruses with bipartite genomes (Howarth and Vandemark, 1989; Etessami et al., 1991; Lazarowitz et al., 1992). This conservation also extends to the AL1 homologs encoded by geminiviruses with a single genome component (Mullineaux et al., 1985; Accotto et al., 1989; Lazarowitz et al., 1989; S ...
bacterial plasmids - Acta Medica Medianae
... exist in supercoiled form, but after alkaling lysis and electrophoresis, they could be found in linear, open circle or multiple-supercoiled form (4). The structure of plasmids is made of circular double chains DNA molecules which are replicated autonomously in a host cell. Their length vary from few ...
... exist in supercoiled form, but after alkaling lysis and electrophoresis, they could be found in linear, open circle or multiple-supercoiled form (4). The structure of plasmids is made of circular double chains DNA molecules which are replicated autonomously in a host cell. Their length vary from few ...
Differential mRNA expression levels and gene sequences of a
... clone from the resistant strain. This nucleotide change results in an amino acid difference in the predicted protein sequence from tryptophan (Trp220) in the susceptible strain to a glycine (Gly220) in the resistant strain. The point mutation at position 658 was confirmed in the genomic DNA sequence ...
... clone from the resistant strain. This nucleotide change results in an amino acid difference in the predicted protein sequence from tryptophan (Trp220) in the susceptible strain to a glycine (Gly220) in the resistant strain. The point mutation at position 658 was confirmed in the genomic DNA sequence ...
PTC Taster Lab Student`s Guide
... up to one day ahead of the second period and stored in a refrigerator, covered in plastic wrap and protected from light. 1. Prepare a clean and dry agarose gel casting tray ● Seal off the ends of the tray as indicated for your apparatus (not needed for blueGel™ users) ● Place a well-forming comb at ...
... up to one day ahead of the second period and stored in a refrigerator, covered in plastic wrap and protected from light. 1. Prepare a clean and dry agarose gel casting tray ● Seal off the ends of the tray as indicated for your apparatus (not needed for blueGel™ users) ● Place a well-forming comb at ...
UNIFR Rusconi 2002
... Gene amplification / manipulation techniques (genetic engineering, recombinant DNA) segments of genomic DNA can be specifically cut and isolated ...
... Gene amplification / manipulation techniques (genetic engineering, recombinant DNA) segments of genomic DNA can be specifically cut and isolated ...
1 BIOL2323: GENERAL GENETICS STUDY GUIDE
... describe the steps of a typical cloning experiment know the meaning of the terms “restriction” and “modification” in bacteria describe how restriction enzyme recognition sites look like and which kinds of DNA ends are produced by restriction enzymes calculate the frequency of restriction sites expla ...
... describe the steps of a typical cloning experiment know the meaning of the terms “restriction” and “modification” in bacteria describe how restriction enzyme recognition sites look like and which kinds of DNA ends are produced by restriction enzymes calculate the frequency of restriction sites expla ...
Construction of plant BAC libraries This document
... 4. Place the mixture on ice for 12 minutes. During this time, swirl very gently the contents of the beaker at least every two minutes (each swirl time = 20 sec). Note : Nuclei in sucrose-based buffers must be handled with extreme care. The absence of divalent cations coupled with the extreme osmot ...
... 4. Place the mixture on ice for 12 minutes. During this time, swirl very gently the contents of the beaker at least every two minutes (each swirl time = 20 sec). Note : Nuclei in sucrose-based buffers must be handled with extreme care. The absence of divalent cations coupled with the extreme osmot ...
Directed Evolution of Polymerases To Accept Nucleotides with
... PCR using the high-fidelity Pfu UltraII (Stratagene) polymerase and 5′-CAGGAAGCAGCCATCAC-3′ as a primer. The products of the secondary PCR were then digested and cloned back into the original vector (a derivative of pASK43IBAplus) using the NcoI and EcoRI restriction sites. The ligated plasmids were ...
... PCR using the high-fidelity Pfu UltraII (Stratagene) polymerase and 5′-CAGGAAGCAGCCATCAC-3′ as a primer. The products of the secondary PCR were then digested and cloned back into the original vector (a derivative of pASK43IBAplus) using the NcoI and EcoRI restriction sites. The ligated plasmids were ...
Clamp loader structure predicts the architecture of DNA polymerase
... accessible to β may be one in which the amino-terminal domain of δ′ interacts more extensively with the aminoterminal, β-interactive, domain of δ. Given very few modeling operations (explained in [17]) a hypothetical ‘closed’ structure can be formed in which the amino-terminal domains of all five su ...
... accessible to β may be one in which the amino-terminal domain of δ′ interacts more extensively with the aminoterminal, β-interactive, domain of δ. Given very few modeling operations (explained in [17]) a hypothetical ‘closed’ structure can be formed in which the amino-terminal domains of all five su ...
Notification of a Notifiable Low Risk Dealing
... 1. Briefly describe the proposed dealing(s) and the purpose of conducting these dealing(s), in the space (box) below. The term 'dealings', in relation to a genetically modified organism (GMO), is defined in the Gene Technology Act 2000 (the Act) as any of the purposes listed below: a. conduct experi ...
... 1. Briefly describe the proposed dealing(s) and the purpose of conducting these dealing(s), in the space (box) below. The term 'dealings', in relation to a genetically modified organism (GMO), is defined in the Gene Technology Act 2000 (the Act) as any of the purposes listed below: a. conduct experi ...
Chapter 8
... Learning Objectives 8-1 Define genetics, genome, chromosome, gene, genetic code, genotype, phenotype, and genomics. 8-2 Describe how DNA serves as genetic information. 8-3 Describe the process of DNA replication. 8-4 Describe protein synthesis, including transcription, RNA processing, and translatio ...
... Learning Objectives 8-1 Define genetics, genome, chromosome, gene, genetic code, genotype, phenotype, and genomics. 8-2 Describe how DNA serves as genetic information. 8-3 Describe the process of DNA replication. 8-4 Describe protein synthesis, including transcription, RNA processing, and translatio ...
Encyclopedia of Nanotechnology
... base-pair steps of RNA polymerase translocation [14] (see Fig. 3c). When viruses are reproduced by infected cells, they are reassembled from proteins which form a shell (i.e., a capsid) and from their genetic material, which they need to infect subsequent cells. The packaging of viral DNA by a rotar ...
... base-pair steps of RNA polymerase translocation [14] (see Fig. 3c). When viruses are reproduced by infected cells, they are reassembled from proteins which form a shell (i.e., a capsid) and from their genetic material, which they need to infect subsequent cells. The packaging of viral DNA by a rotar ...
Defined, consistent quality SYNTHETIC BIOLOGY
... unicellular photosynthetic prokaryotes sometimes referred to as blue-green algae. S. elongatus is an excellent model system with the following features: • Fully sequenced and annotated genome (strain PCC 7942) • Easily manipulated by transformation or conjugation from E. coli • Small genome siz ...
... unicellular photosynthetic prokaryotes sometimes referred to as blue-green algae. S. elongatus is an excellent model system with the following features: • Fully sequenced and annotated genome (strain PCC 7942) • Easily manipulated by transformation or conjugation from E. coli • Small genome siz ...
Lack of homology between two haloacetate dehalogenase genes
... The diversity of the dehalogenases may result from selection for micro-organismsable to degrade a variety of novel halogenated compounds. Enzyme evolution may be initiated by tandem duplication of a gene, followed by the accumulation of multiple mutations on either gene copy, which results in the cr ...
... The diversity of the dehalogenases may result from selection for micro-organismsable to degrade a variety of novel halogenated compounds. Enzyme evolution may be initiated by tandem duplication of a gene, followed by the accumulation of multiple mutations on either gene copy, which results in the cr ...
Original Article:
... located in the anterior nares of the patient. The understanding of the biological nature of S. aureus colonization is still limited, and most studies of the nasal carriage presume that individuals are colonized by a single strain. Recently simultaneous nasal carriage of multiple strains of S. aureus ...
... located in the anterior nares of the patient. The understanding of the biological nature of S. aureus colonization is still limited, and most studies of the nasal carriage presume that individuals are colonized by a single strain. Recently simultaneous nasal carriage of multiple strains of S. aureus ...
Biology Revised
... (ii) A section of a DNA molecule containing a total of 1600 bases has 184 adenine and 216 thymine bases on one strand. The complementary strand contains 268 cytosine bases. 1 Calculate the number of adenine bases in this whole section of the DNA molecule. Space for calculation ...
... (ii) A section of a DNA molecule containing a total of 1600 bases has 184 adenine and 216 thymine bases on one strand. The complementary strand contains 268 cytosine bases. 1 Calculate the number of adenine bases in this whole section of the DNA molecule. Space for calculation ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.