Norsk rapport - Forsvarets forskningsinstitutt
... FFI has established several real-time PCR assays for various biological agents [6]. Also a method for identifying Vibrio cholerae has been established [7]. ...
... FFI has established several real-time PCR assays for various biological agents [6]. Also a method for identifying Vibrio cholerae has been established [7]. ...
'This day designing God Hath put into my hand
... named malaria toxins. The proposed main consequence of these bio-active molecules is to direct the systemic release of several pro-inflammatory cytokines, in particular tumour necrosis factor
... named malaria toxins. The proposed main consequence of these bio-active molecules is to direct the systemic release of several pro-inflammatory cytokines, in particular tumour necrosis factor
Developmental Validation of the Quantifiler Real-Time
... formula was determined using Microsoft Excel 2000. Each sample was then quantified by entering its background-subtracted numerical fluorescence value into the regression formula and solving for DNA concentration. Slot blot hybridization DNA quantification assays were perR formed using the QuantiBlo ...
... formula was determined using Microsoft Excel 2000. Each sample was then quantified by entering its background-subtracted numerical fluorescence value into the regression formula and solving for DNA concentration. Slot blot hybridization DNA quantification assays were perR formed using the QuantiBlo ...
Transcription
... Figure 4 RNA is synthesized at a moving transcription bubble (Figure 4). The two strands of the bubble are known as the template and the non-template strand. RNA is copied from the template strand. The region of strand separation moves down the double helix with continual unwinding and rewinding of ...
... Figure 4 RNA is synthesized at a moving transcription bubble (Figure 4). The two strands of the bubble are known as the template and the non-template strand. RNA is copied from the template strand. The region of strand separation moves down the double helix with continual unwinding and rewinding of ...
Physical map of the aromatic amine and m-toluate
... Plasmid DNA extraction and manipulation. Presumptive Tn5 insertion mutants were screened using the procedure of Birnboim & Doly (1979); detailed analysis was done on plasmid DNA extracted by the procedure of Wheatcroft & Williams (1981). Screening for the presence of recombinant plasmids was done ac ...
... Plasmid DNA extraction and manipulation. Presumptive Tn5 insertion mutants were screened using the procedure of Birnboim & Doly (1979); detailed analysis was done on plasmid DNA extracted by the procedure of Wheatcroft & Williams (1981). Screening for the presence of recombinant plasmids was done ac ...
p53
... Discovery of p53 - SV40 DNA tumor virus transforms monkey kidney cells into cancerous cells - SV40 DNA tumor virus can also transform murine cells in culture - The virus is injected into hamsters and the serum is used to precipitate the oncogenic protein: Large T antigen - … and another protein, ~5 ...
... Discovery of p53 - SV40 DNA tumor virus transforms monkey kidney cells into cancerous cells - SV40 DNA tumor virus can also transform murine cells in culture - The virus is injected into hamsters and the serum is used to precipitate the oncogenic protein: Large T antigen - … and another protein, ~5 ...
Ernest Just - CPO Science
... Write the decimal from (4) as a mixed number, and reduce to lowest terms if possible. 1.8 = 1 8/10 = 1 4/5 ...
... Write the decimal from (4) as a mixed number, and reduce to lowest terms if possible. 1.8 = 1 8/10 = 1 4/5 ...
Detection of Genetically Altered Corn and Soybean Food Products
... A subset of PCR corn samples is shown in this gel. Read these samples results in pair lanes (3 and 4; 5 and 6; 7 and 8). Lane 1: 100 base pairs ladder. Lane 2: No DNA, negative control. Lane 3: Moss’ corn meal specie specific PCR reaction. Lane 4: Moss’ corn meal genetic modification PCR reaction. L ...
... A subset of PCR corn samples is shown in this gel. Read these samples results in pair lanes (3 and 4; 5 and 6; 7 and 8). Lane 1: 100 base pairs ladder. Lane 2: No DNA, negative control. Lane 3: Moss’ corn meal specie specific PCR reaction. Lane 4: Moss’ corn meal genetic modification PCR reaction. L ...
High throughput nucleic acid sample preparation in 96 well plates
... For purification of viral DNA or RNA the InviMag® Universal Kit/ STARlet, Invisorb® Universal HTS 96 Kit/ STARlet and for purification of viral RNA the Invisorb® Virus RNA HTS 96 Kit/ STARlet or InviMag® Virus RNA Kit/ STARlet are available. Please request the info material. *) Products which are CE ...
... For purification of viral DNA or RNA the InviMag® Universal Kit/ STARlet, Invisorb® Universal HTS 96 Kit/ STARlet and for purification of viral RNA the Invisorb® Virus RNA HTS 96 Kit/ STARlet or InviMag® Virus RNA Kit/ STARlet are available. Please request the info material. *) Products which are CE ...
ACLS CH05 - CTCE Moodle
... inserted into plasmids, forming recombinants that are placed into bacterial host cells. Bacteria containing the plasmids grow on an agar medium, producing a colony of cells containing the cloned gene. ...
... inserted into plasmids, forming recombinants that are placed into bacterial host cells. Bacteria containing the plasmids grow on an agar medium, producing a colony of cells containing the cloned gene. ...
p53 regulation and function in normal cells and tumors
... within promoter regions of growth inhibitory genes. However, p53 also binds sequence-independently to damaged sites in DNA and is postulated to have a role in DNA repair or apoptosis. Mutations of p53 have been found in more than 50% of human cancers1. However, loss of p53 function has been estimate ...
... within promoter regions of growth inhibitory genes. However, p53 also binds sequence-independently to damaged sites in DNA and is postulated to have a role in DNA repair or apoptosis. Mutations of p53 have been found in more than 50% of human cancers1. However, loss of p53 function has been estimate ...
Ab Initio Modeling of Biological Systems - Psi-k
... However, there are many areas in which the use of effective potentials may be not appropriate, and more sophisticated and accurate approaches are required. An alternative is offered by ab initio molecular dynamics (AIMD). AIMD in the Born-Oppheneimer approximation can be efficiently performed using ...
... However, there are many areas in which the use of effective potentials may be not appropriate, and more sophisticated and accurate approaches are required. An alternative is offered by ab initio molecular dynamics (AIMD). AIMD in the Born-Oppheneimer approximation can be efficiently performed using ...
The Basics of RT-PCR
... tubes in the same run. This type of contamination is thus insidious, because the negative control tubes are often “clean;” therefore, unexpected positive results can be caused simply by contamination. If a contamination problem is suspected, it is sometimes helpful to perform multiple negative-contr ...
... tubes in the same run. This type of contamination is thus insidious, because the negative control tubes are often “clean;” therefore, unexpected positive results can be caused simply by contamination. If a contamination problem is suspected, it is sometimes helpful to perform multiple negative-contr ...
Molecular cloning and functional characterisation of a glucose
... transporter genes (HXTs) of S. cerevisiae [17,18] and was found to be localised on chromosome I. In order to clone the complete ORF, the corresponding fosmid library of C. albicans [19], containing chromosome I fragments, was screened using the 1-kb EcoRI-HindIII subfragment of pAV1 (pAV4, Fig. 1A) ...
... transporter genes (HXTs) of S. cerevisiae [17,18] and was found to be localised on chromosome I. In order to clone the complete ORF, the corresponding fosmid library of C. albicans [19], containing chromosome I fragments, was screened using the 1-kb EcoRI-HindIII subfragment of pAV1 (pAV4, Fig. 1A) ...
TRANSPOSON INSERTION SITE VERIFICATION
... recommend that you BLAST the primer sequences against the Arabidopsis genome sequence to confirm their specificity for the target region; The insertion site specific primers designed (in this case SMF & SMR) will be used in a 3 primer PCR reaction. This will verify the insertion site and to confirm ...
... recommend that you BLAST the primer sequences against the Arabidopsis genome sequence to confirm their specificity for the target region; The insertion site specific primers designed (in this case SMF & SMR) will be used in a 3 primer PCR reaction. This will verify the insertion site and to confirm ...
Biotechnology in Livestock Improvement
... structure (i.e., shaped like a corkscrew). Their double-helix model had two strands of DNA with the nucleotides pointing inward, each matching a complementary nucleotide on the other strand to form what looks like rungs on a twisted ladder. This structure showed that genetic information exists in th ...
... structure (i.e., shaped like a corkscrew). Their double-helix model had two strands of DNA with the nucleotides pointing inward, each matching a complementary nucleotide on the other strand to form what looks like rungs on a twisted ladder. This structure showed that genetic information exists in th ...
The Process of How COMT Genetic Variants Provide the
... the DNA code are significant enough to differentiate every human being. The COMT gene is a small portion of a human DNA sequence that provides instructions on how to build the COMT enzyme. The COMT enzyme, a molecule that changes the shape of dopamine, is a critically important protein that regulate ...
... the DNA code are significant enough to differentiate every human being. The COMT gene is a small portion of a human DNA sequence that provides instructions on how to build the COMT enzyme. The COMT enzyme, a molecule that changes the shape of dopamine, is a critically important protein that regulate ...
Genetics_Review_Jeopardy_
... During this process the DNA strands separate and one DNA strand is used to make mRNA, and during this process mRNA is used to produce proteins. What are transcription and translation? ...
... During this process the DNA strands separate and one DNA strand is used to make mRNA, and during this process mRNA is used to produce proteins. What are transcription and translation? ...
Genetic Markers for Sex Identification in Forensic DNA Analysis
... marker is required for relatives of missing persons, along with the 13 CODIS Core Loci [1]. The amelogenin test must be attempted for DNA profiles of missing persons and unidentified human remains, and is accepted but not required for offender and forensic profiles [1]. In addition to amelogenin, al ...
... marker is required for relatives of missing persons, along with the 13 CODIS Core Loci [1]. The amelogenin test must be attempted for DNA profiles of missing persons and unidentified human remains, and is accepted but not required for offender and forensic profiles [1]. In addition to amelogenin, al ...
Repressing Integrase attachment site operation
... extracted from the host’s genome [1]–[3]. These enzymes are particularly interesting and useful to synthetic biologists because they are inherently directional. A serine integrase tetramer will catalyze a recombination event between two heterotypic DNA “attachment” sites (attP and attB) [4]. Without ...
... extracted from the host’s genome [1]–[3]. These enzymes are particularly interesting and useful to synthetic biologists because they are inherently directional. A serine integrase tetramer will catalyze a recombination event between two heterotypic DNA “attachment” sites (attP and attB) [4]. Without ...
ppt presentation
... Repetitions are serious problem in assembly, if they are conserved and longer than sequencing run ...
... Repetitions are serious problem in assembly, if they are conserved and longer than sequencing run ...
How to measure chromatin modifications
... first step is the purification of modified chromatin by immunoprecipitation using an antibody that is specific to a particular histone modification (shown in green). The ChIP DNA ends are repaired and ligated to a pair of adaptors, followed by limited PCR amplification. The DNA molecules are bound t ...
... first step is the purification of modified chromatin by immunoprecipitation using an antibody that is specific to a particular histone modification (shown in green). The ChIP DNA ends are repaired and ligated to a pair of adaptors, followed by limited PCR amplification. The DNA molecules are bound t ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.