... to a 100 basepair strand of DNA is measured. In this case the protein is considered the ligand (L) and the DNA is considered the macromolecule (M); more than one protein can bind to a single DNA molecule. The fractional saturation as a function of the protein concentration is shown below, measured a ...
RecA maintains the integrity of chloroplast DNA molecules in
... nuclear genomes of many organisms, including plants (Lin et al., 2006). Repair and recombination of chloroplast DNA (cpDNA) in Chlamydomonas reinhardtii is suppressed when a dominant-negative version of E. coli RecA is targeted to chloroplasts (Cerutti et al., 1995). The prevalence of chloroplast-ta ...
... nuclear genomes of many organisms, including plants (Lin et al., 2006). Repair and recombination of chloroplast DNA (cpDNA) in Chlamydomonas reinhardtii is suppressed when a dominant-negative version of E. coli RecA is targeted to chloroplasts (Cerutti et al., 1995). The prevalence of chloroplast-ta ...
microbial genetics
... maintenance mechanisms, and drug resistance and colicin production. In bacteria, plasmid transfer occurs through transformation and conjugation. Often it is useful to transfer a nontransmissible plasmid to a specific host cell. It is possible to transfer the purified DNA as long as a genetic selecti ...
... maintenance mechanisms, and drug resistance and colicin production. In bacteria, plasmid transfer occurs through transformation and conjugation. Often it is useful to transfer a nontransmissible plasmid to a specific host cell. It is possible to transfer the purified DNA as long as a genetic selecti ...
Expression of pBLU
... Bacteria on an agar plate containing the antibiotic ampicillin will only grow if they have received the plasmid. If the agar plate also contains X-gal, the -galactosidase gene product will convert X-gal into Indo-Blu, our dye. E. coli colonies are usually white. Those producing Indo-Blu will be blu ...
... Bacteria on an agar plate containing the antibiotic ampicillin will only grow if they have received the plasmid. If the agar plate also contains X-gal, the -galactosidase gene product will convert X-gal into Indo-Blu, our dye. E. coli colonies are usually white. Those producing Indo-Blu will be blu ...
DNA copy number analysis by MAPH: molecular diagnostic
... designed to have the same primer sites Figure 1. Principle of multiplex amplifiable probe hybridization (MAPH). Diagram illustrating the important steps in MAPH. Amplifiable probes are shown, representing the different loci tested, with flanking the locus-specific sequence. To each sharing a common ...
... designed to have the same primer sites Figure 1. Principle of multiplex amplifiable probe hybridization (MAPH). Diagram illustrating the important steps in MAPH. Amplifiable probes are shown, representing the different loci tested, with flanking the locus-specific sequence. To each sharing a common ...
Chpt20_TxnlRegChromatin.doc
... nuclei. This shows that the amount of globin gene DNA in erythroid nuclei is substantially reduced by the DNase I treatment, i.e. the globin gene is sensitive to DNase I in a cell that is expressing it. {To put a finer touch on it, the erythrocytes are descended from cells that were actively express ...
... nuclei. This shows that the amount of globin gene DNA in erythroid nuclei is substantially reduced by the DNase I treatment, i.e. the globin gene is sensitive to DNase I in a cell that is expressing it. {To put a finer touch on it, the erythrocytes are descended from cells that were actively express ...
CHAPTER 4 ISOLATION, CHARACTERIZATION AND EXPRESSION OF GA20ox
... DNA sequences were analyzed using the BLAST program (http://www.ncbi.nlm.nih.gov /blast/) by comparing with sequences in the GenBank database. Candidate nucleotide sequences were translated using the ExPASY Translation Tool (http://au.expasy. org/ tools/dna. html) before BLAST search for determining ...
... DNA sequences were analyzed using the BLAST program (http://www.ncbi.nlm.nih.gov /blast/) by comparing with sequences in the GenBank database. Candidate nucleotide sequences were translated using the ExPASY Translation Tool (http://au.expasy. org/ tools/dna. html) before BLAST search for determining ...
paper - Lirmm
... sequence that is most likely to encode that protein. Several programs use multiple protein alignments to improve the back-translation [12,13]. This can be considered to be the opposite to the “translation way”, where translation is used to improve coding DNA alignments or assess new coding DNA [14-1 ...
... sequence that is most likely to encode that protein. Several programs use multiple protein alignments to improve the back-translation [12,13]. This can be considered to be the opposite to the “translation way”, where translation is used to improve coding DNA alignments or assess new coding DNA [14-1 ...
Brief Rapid Communications
... therapy. First, this vector can only accept transgenes less than '4.5 kb in length. Second, current techniques do not allow the convenient production of large amounts of rAAV. Finally, although our studies have demonstrated efficient transduction of cardiomyocytes after 15 minutes of coronary artery ...
... therapy. First, this vector can only accept transgenes less than '4.5 kb in length. Second, current techniques do not allow the convenient production of large amounts of rAAV. Finally, although our studies have demonstrated efficient transduction of cardiomyocytes after 15 minutes of coronary artery ...
Genome demethylation and imprinting in the endosperm
... Angiosperm seeds are composed of the seed coat, the mature embryo, and the endosperm. The endosperm, which serves as a conduit to store and transport nutrients to the embryo during initial phases of seed growth, is a major food source for most of the world. With one exception [1], all known plant g ...
... Angiosperm seeds are composed of the seed coat, the mature embryo, and the endosperm. The endosperm, which serves as a conduit to store and transport nutrients to the embryo during initial phases of seed growth, is a major food source for most of the world. With one exception [1], all known plant g ...
Block 1: Genetics Dr. McKinney Test 1: Transcription (4) The order
... iii. rRNA (a) 1. It’s important to not that all have the same mechanism of action but different types of promoters, and therefore, different transcripts. Also, cell mitochondria have their own RNA polymerases. In contrast, Prokaryotes only have ONE RNA pol to generate ALL types of RNA (m,t,rRNA). Th ...
... iii. rRNA (a) 1. It’s important to not that all have the same mechanism of action but different types of promoters, and therefore, different transcripts. Also, cell mitochondria have their own RNA polymerases. In contrast, Prokaryotes only have ONE RNA pol to generate ALL types of RNA (m,t,rRNA). Th ...
Identification of Mga1, a G‐protein α‐subunit gene involved in
... have been identified (Shimizu et al., 2007; Chen et al., 2008b). Based on the genetic information, a genetic modification method has also been proposed (Fu et al., 2007; Jia et al., 2010). Secondary metabolite production is controlled at an upper hierarchical level by many global mechanisms, in whic ...
... have been identified (Shimizu et al., 2007; Chen et al., 2008b). Based on the genetic information, a genetic modification method has also been proposed (Fu et al., 2007; Jia et al., 2010). Secondary metabolite production is controlled at an upper hierarchical level by many global mechanisms, in whic ...
science - Madison Central High
... Alkaptonuria was the first disease linked to genes Garrod called this disease “an inborn error of metabolism” in a 1908 report Genetic diseases such as alkaptonuria are carried on recessive alleles Thomas Hunt Morgan was the first geneticist to study animals Mendel had only conducted studies ...
... Alkaptonuria was the first disease linked to genes Garrod called this disease “an inborn error of metabolism” in a 1908 report Genetic diseases such as alkaptonuria are carried on recessive alleles Thomas Hunt Morgan was the first geneticist to study animals Mendel had only conducted studies ...
Unit 05 - Delivery guide
... Where two or more genes at different loci interact. This interaction could produce a new phenotype, cause one allele to mask the effects of another/ others, or one allele to modify the effects of another/others. ...
... Where two or more genes at different loci interact. This interaction could produce a new phenotype, cause one allele to mask the effects of another/ others, or one allele to modify the effects of another/others. ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... Yeast transformation:Yeast transformation by the spheroplasting method was performed essentially as described by HINNEN,HICKSand FINK(1978) with the exception that STC buffer [ 1 M sorbitol, 10 mM Tris (pH 7.5), 10 mM CaCI2] was substituted for 1 M sorbitol in the third wash aftertreatment with glus ...
... Yeast transformation:Yeast transformation by the spheroplasting method was performed essentially as described by HINNEN,HICKSand FINK(1978) with the exception that STC buffer [ 1 M sorbitol, 10 mM Tris (pH 7.5), 10 mM CaCI2] was substituted for 1 M sorbitol in the third wash aftertreatment with glus ...
GF-1 Food DNA Extraction Kit
... genomic DNA from up to 100mg of raw or processed food from plant, animal or mixed origins. This kit uses a specially-treated glass filter membrane fixed into a column to efficiently bind DNA in the presence of high salt. The kit applies the principle of a minicolumn spin technology and the use of op ...
... genomic DNA from up to 100mg of raw or processed food from plant, animal or mixed origins. This kit uses a specially-treated glass filter membrane fixed into a column to efficiently bind DNA in the presence of high salt. The kit applies the principle of a minicolumn spin technology and the use of op ...
Copy of NAR30_7.book(gkf263.fm)
... conjugates prepared from it. These DNA–gold nanoparticle conjugates exhibit substantially higher stability than analogs prepared from monothiol and cyclic disulfide-capped oligodeoxyribonucleotides, but comparable hybridization properties. A quantitative analysis of their stability under a range of ...
... conjugates prepared from it. These DNA–gold nanoparticle conjugates exhibit substantially higher stability than analogs prepared from monothiol and cyclic disulfide-capped oligodeoxyribonucleotides, but comparable hybridization properties. A quantitative analysis of their stability under a range of ...
NEHRU ARTS AND SCIENCE COLLEGE DEPARTMENT OF
... new explants might reduce variability over time. Another way of reducing somaclonal variation is to avoid 2,4-D in the culture medium, as this hormone is known to introduce variation.Vitrification[hyperhydracity] may be a problem in some species. In case of forest trees, mature elite trees can be id ...
... new explants might reduce variability over time. Another way of reducing somaclonal variation is to avoid 2,4-D in the culture medium, as this hormone is known to introduce variation.Vitrification[hyperhydracity] may be a problem in some species. In case of forest trees, mature elite trees can be id ...
A structural determinant in the uracil DNA glycosylase superfamily
... 1 uracil N-glycosylase (UNG) from E. coli is an extremely efficient enzyme that can remove uracil from any uracil-containing base pairs including the A/U base pair. Here, we report the identification of an important structural determinant that underlies the functional difference between MUG and UNG. ...
... 1 uracil N-glycosylase (UNG) from E. coli is an extremely efficient enzyme that can remove uracil from any uracil-containing base pairs including the A/U base pair. Here, we report the identification of an important structural determinant that underlies the functional difference between MUG and UNG. ...
Comparison of DNA Sequences with Protein Sequences
... TFASTX/Y use a similar strategy, but instead of augmenting the query-sequence lookup table, the library sequence is encoded as two separate three-frame translations, one forward and one reverse. Again, steps 3 and 4 are modified for DNA–protein comparison and TFASTX/Y provide a full Smith–Waterman a ...
... TFASTX/Y use a similar strategy, but instead of augmenting the query-sequence lookup table, the library sequence is encoded as two separate three-frame translations, one forward and one reverse. Again, steps 3 and 4 are modified for DNA–protein comparison and TFASTX/Y provide a full Smith–Waterman a ...
Chapter 1 The Framework of Biology
... The genetic material of prokaryotes is in the form of a circular DNA along with smaller pieces called plasmids. Many prokaryotes have flagella which propel them through their environment. Some prokaryotes have a slimy sheath on the outside of their capsule. Being small solves many problems. Gas diff ...
... The genetic material of prokaryotes is in the form of a circular DNA along with smaller pieces called plasmids. Many prokaryotes have flagella which propel them through their environment. Some prokaryotes have a slimy sheath on the outside of their capsule. Being small solves many problems. Gas diff ...
A-10484A SNPs. Mutations and DNA Sequence
... using a panel of 10 SNPs. PCR products were obtained for each individual SNP as separate reactions. Primers were designed so that none of the products exceeded 1000 bps in size. During design they were also masked for repeats using the human repeat database. PCR products were verified on a agarose g ...
... using a panel of 10 SNPs. PCR products were obtained for each individual SNP as separate reactions. Primers were designed so that none of the products exceeded 1000 bps in size. During design they were also masked for repeats using the human repeat database. PCR products were verified on a agarose g ...
Mapping the histone code at hMLH1. - JScholarship
... modifications examined were exclusive to either histone, H3.3 was shown to be enriched with modifications that are associated with transcriptionally active genes whereas H3 was shown to be enriched with methyl-H3-K9, the modification associated with silent genes (McKittrick et al., 2004). There are, ...
... modifications examined were exclusive to either histone, H3.3 was shown to be enriched with modifications that are associated with transcriptionally active genes whereas H3 was shown to be enriched with methyl-H3-K9, the modification associated with silent genes (McKittrick et al., 2004). There are, ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.