... were isolated with a tightly linked RAPD marker, and subsequently the regions flanking the Rps1 locus were nearly saturated with AFLP markers. Identification and mapping of RAPD markers linked to the Rps1 locus. The screening of 400 decamer primers against the cultivar Williams (rps1-k) and its NIL ...
Semiconservative Replication in the Quasispecies Model
... not necessarily paired with σ̄. Rather, once cell division is finished, the original σ is paired with some σ ′ , and similarly for σ̄. Each genome {σ, σ̄} has a characteristic replication mismatch probability ǫ{σ,σ̄} (a base-pair-independent mismatch probability is certainly a simplification, but it ...
... not necessarily paired with σ̄. Rather, once cell division is finished, the original σ is paired with some σ ′ , and similarly for σ̄. Each genome {σ, σ̄} has a characteristic replication mismatch probability ǫ{σ,σ̄} (a base-pair-independent mismatch probability is certainly a simplification, but it ...
Cloning and Effective Induction of Escherichia coli
... was extracted with equal volume of STET-saturated phenol. To the aqueous layer, 0.1 volume of 4 M lithium chloride was added and placed on ice for 5 min. After centrifugation at 10,000 rpm, equal volume of isopropanol was added to supernatant. Chromosomal DNA was recovered by centrifugation at 10,00 ...
... was extracted with equal volume of STET-saturated phenol. To the aqueous layer, 0.1 volume of 4 M lithium chloride was added and placed on ice for 5 min. After centrifugation at 10,000 rpm, equal volume of isopropanol was added to supernatant. Chromosomal DNA was recovered by centrifugation at 10,00 ...
Kein Folientitel
... commercial exploitation would be contrary to ordre public or morality; however, exploitation shall not be deemed to be so contrary merely because it is prohibited by law or regulation. 2. On the basis of paragraph 1, the following, in particular, shall be considered unpatentable: (a) processes for c ...
... commercial exploitation would be contrary to ordre public or morality; however, exploitation shall not be deemed to be so contrary merely because it is prohibited by law or regulation. 2. On the basis of paragraph 1, the following, in particular, shall be considered unpatentable: (a) processes for c ...
doc
... Database-mining: this is the process by which the structure/function of an unknown gene/protein is inferred from similar sequences identified in information already stored in database, most often from well-characterised model organisms. Evolutionary Biology: BI offers the potential for investigating ...
... Database-mining: this is the process by which the structure/function of an unknown gene/protein is inferred from similar sequences identified in information already stored in database, most often from well-characterised model organisms. Evolutionary Biology: BI offers the potential for investigating ...
Genetic Engineering of Plants for Resistance to Viruses
... leaves, roots and stem internodes. Somatic embryogenesis can be induced from axillary bud shoot tips, apical and bud meristems, and leaf, petiole, stem and root explants Methods of sweet potato transformation Electroporation of protoplasts (Nishiguchi et al., 1992) Agrobacterium-mediated transformat ...
... leaves, roots and stem internodes. Somatic embryogenesis can be induced from axillary bud shoot tips, apical and bud meristems, and leaf, petiole, stem and root explants Methods of sweet potato transformation Electroporation of protoplasts (Nishiguchi et al., 1992) Agrobacterium-mediated transformat ...
Biology 343 Lab (Dorn, Shade)
... OBJECTIVES: Welcome to the laboratory portion of Biology 343. This course is designed to give you hands-on experience with modern genetics techniques. It is not designed to coincide very much with your lecture material; in fact, it could be a course of its own, which is what may happen to it someday ...
... OBJECTIVES: Welcome to the laboratory portion of Biology 343. This course is designed to give you hands-on experience with modern genetics techniques. It is not designed to coincide very much with your lecture material; in fact, it could be a course of its own, which is what may happen to it someday ...
Part III: Laboratory – Electrophoresis
... TEXT FOR PRELAB PREP Lab Flow and Summary Note: You must plant the arabidopsis seed that comes with the kit 3-4 weeks prior to doing the lab. See instructions for planting and growing seed. The lab can be broken into three parts: ...
... TEXT FOR PRELAB PREP Lab Flow and Summary Note: You must plant the arabidopsis seed that comes with the kit 3-4 weeks prior to doing the lab. See instructions for planting and growing seed. The lab can be broken into three parts: ...
DNA and the Book of Mormon: A Phylogenetic Perspective
... fleas to understanding the population genetics of crayfish, DNA sequence information can provide clues to the past and allow scientists to test very specific hypotheses in a way that was unapproachable even a few years ago. The announced completion of the Human Genome Project is not really a complet ...
... fleas to understanding the population genetics of crayfish, DNA sequence information can provide clues to the past and allow scientists to test very specific hypotheses in a way that was unapproachable even a few years ago. The announced completion of the Human Genome Project is not really a complet ...
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... orthologue of ATM and was distinct from internal γ-H2A formation, which depended mostly on the orthologue of ATR (22). It had been thought that telomeres should be protected from recognition as DSBs to prevent cell cycle arrest but it was recently discovered that telomeres could prevent cell cycle d ...
... orthologue of ATM and was distinct from internal γ-H2A formation, which depended mostly on the orthologue of ATR (22). It had been thought that telomeres should be protected from recognition as DSBs to prevent cell cycle arrest but it was recently discovered that telomeres could prevent cell cycle d ...
Significant enhancement of fatty acid composition in seeds of the
... disruptions altering the gene’s reading frame would produce a translation product lacking enzymatic activity. The sites were also chosen to contain a restriction enzyme cut site at the site of Cas9/ sgRNA-directed DNA cleavage three base pairs upstream of the PAM site. As described below, if Cas9/sg ...
... disruptions altering the gene’s reading frame would produce a translation product lacking enzymatic activity. The sites were also chosen to contain a restriction enzyme cut site at the site of Cas9/ sgRNA-directed DNA cleavage three base pairs upstream of the PAM site. As described below, if Cas9/sg ...
Immobilization_Mecha..
... (30%), 11-mercaptoundecylphosphoric acid, 1-undecanethiol, ammonium hydroxide, 20× SSC (saline sodium citrate) buffer, poly-(α,β)DL-aspartic acid sodium salt, and poly-L-lysine hydrobromide were obtained from Sigma-Aldrich (St. Louis, MO). Hafnium dichloride oxide octahydrate was obtained from Alfa A ...
... (30%), 11-mercaptoundecylphosphoric acid, 1-undecanethiol, ammonium hydroxide, 20× SSC (saline sodium citrate) buffer, poly-(α,β)DL-aspartic acid sodium salt, and poly-L-lysine hydrobromide were obtained from Sigma-Aldrich (St. Louis, MO). Hafnium dichloride oxide octahydrate was obtained from Alfa A ...
Virginia State Science Olympiad Regional Tournament
... Two (2) non-programmable non-graphing calculators, one (1) standard-size (8.5”x11”) double-sided sheet (not two one-side sheets) of paper containing any information, and writing implements may be used. Any other electronic devices are not allowed for this event; please consult the proctor about a sa ...
... Two (2) non-programmable non-graphing calculators, one (1) standard-size (8.5”x11”) double-sided sheet (not two one-side sheets) of paper containing any information, and writing implements may be used. Any other electronic devices are not allowed for this event; please consult the proctor about a sa ...
Molecular Phylogenetics Basis of Phylogenetics
... all organisms have evolved, and continue to evolve through time along divergent lines ...
... all organisms have evolved, and continue to evolve through time along divergent lines ...
Add Health Biomarker - Carolina Population Center
... required that 15-20 cc of first stream urine be collected in a plastic, preservative-free, sterile urine specimen collection cup from respondents who have not urinated within one hour prior to collection, and that the specimen be cooled immediately to 2-8ºC until processed. Respondents received inst ...
... required that 15-20 cc of first stream urine be collected in a plastic, preservative-free, sterile urine specimen collection cup from respondents who have not urinated within one hour prior to collection, and that the specimen be cooled immediately to 2-8ºC until processed. Respondents received inst ...
pdf
... propagated by infection of other individuals. This may be a natural means for evolving new strains of viruses. One of the most striking examples is the acquisition and modification of a protooncogene, such as cellular c-src, by a retrovirus to generate a modified, transforming form of the gene, call ...
... propagated by infection of other individuals. This may be a natural means for evolving new strains of viruses. One of the most striking examples is the acquisition and modification of a protooncogene, such as cellular c-src, by a retrovirus to generate a modified, transforming form of the gene, call ...
The global repressor FliZ antagonizes gene
... play a central role in switching from the planktonic and motile life-style to the adhesive state during the transition from post-exponential growth to early stationary phase. Two modes of regulation contribute to the inverse coordination of flagellum-based motility and curliassociated adhesion in E. ...
... play a central role in switching from the planktonic and motile life-style to the adhesive state during the transition from post-exponential growth to early stationary phase. Two modes of regulation contribute to the inverse coordination of flagellum-based motility and curliassociated adhesion in E. ...
FREE Sample Here - Test bank Store
... 45) For geneticists, why is it important that genetic variability exist in the population under study? Answer: Genetic variation in individuals of a population is important for studying the inheritance pattern of those characteristics. If all the members of a population were identical for the trait ...
... 45) For geneticists, why is it important that genetic variability exist in the population under study? Answer: Genetic variation in individuals of a population is important for studying the inheritance pattern of those characteristics. If all the members of a population were identical for the trait ...
Archives of Microbiology 167:
... Upon subsequent isolation and characterization, three cosmids showed similar restriction patterns in Southern hybridizations. We attempted to clone several larger genomic fragments (5.5-12 kb) from cosmid pSCR213 into pBluescript and/or pACYC177 without success. Most clones analyzed from these ligat ...
... Upon subsequent isolation and characterization, three cosmids showed similar restriction patterns in Southern hybridizations. We attempted to clone several larger genomic fragments (5.5-12 kb) from cosmid pSCR213 into pBluescript and/or pACYC177 without success. Most clones analyzed from these ligat ...
The Development of a Genetic Marker for Resistance to Fescue
... performed by a University of Tennessee Extension Veterinarian. Genomic DNA was isolated from the tail hair follicles of each animal using Quickextract (Epicentre, Cambridge, UK). A genomic amplification was then performed for the isolated DNA samples using the GenomiPhi DNA amplification kit (GE Hea ...
... performed by a University of Tennessee Extension Veterinarian. Genomic DNA was isolated from the tail hair follicles of each animal using Quickextract (Epicentre, Cambridge, UK). A genomic amplification was then performed for the isolated DNA samples using the GenomiPhi DNA amplification kit (GE Hea ...
Molecular identification of tick-borne pathogens in
... Abstract: Tick-borne diseases are one of the most important classes of disease in animal husbandry and cause severe economic losses. In this study, 660 adult female ticks were collected from sheep in nine localities in Henan Province, China. All were identified as Haemaphysalis longicornis, confirme ...
... Abstract: Tick-borne diseases are one of the most important classes of disease in animal husbandry and cause severe economic losses. In this study, 660 adult female ticks were collected from sheep in nine localities in Henan Province, China. All were identified as Haemaphysalis longicornis, confirme ...
Fish-on-a-chip: a sensitive detection microfluidic system for
... microfluidic chip-based FISH techniques, as only a few minutes are required to complete this procedure. A FISH-based microfluidic chip device can analyze thousands of genes or thousands of patient samples at a single time, and a technician only needs to spend a few minutes [69]. Many types of analyz ...
... microfluidic chip-based FISH techniques, as only a few minutes are required to complete this procedure. A FISH-based microfluidic chip device can analyze thousands of genes or thousands of patient samples at a single time, and a technician only needs to spend a few minutes [69]. Many types of analyz ...
Genotyping BoLA-DRB3 alleles in Brazilian Dairy Gir cattle (Bos
... reaction and restriction fragment length polymorphism of the amplified fragments (PCR-RFLP) for assignment of alleles. This methodology cannot accurately determine differences between all current alleles, and this may have led to the different conclusions in disease association studies. Another tech ...
... reaction and restriction fragment length polymorphism of the amplified fragments (PCR-RFLP) for assignment of alleles. This methodology cannot accurately determine differences between all current alleles, and this may have led to the different conclusions in disease association studies. Another tech ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.