The Maintenance and Propagation of Plasmid Genes in Bacterial
... two obvious possibilities present themselves. Either the plasmid could co-segregate with the chromosome (for example, as part of the nucleoid), or it could actively segregate independently via a membrane attachment site. Multicopy plasmids, in principle, can use an alternative strategy since their c ...
... two obvious possibilities present themselves. Either the plasmid could co-segregate with the chromosome (for example, as part of the nucleoid), or it could actively segregate independently via a membrane attachment site. Multicopy plasmids, in principle, can use an alternative strategy since their c ...
Genomic and Physiological Comparisons Between Heterotrophic
... insight is supported by the homology results shown in Table 1. If the two organisms differ by one or a few mutations, their DNAs should show nearly 100% homology (within the experimental precision of the method used, which is -5 to 8% homology). However, data in Table 1 do indicate a close relations ...
... insight is supported by the homology results shown in Table 1. If the two organisms differ by one or a few mutations, their DNAs should show nearly 100% homology (within the experimental precision of the method used, which is -5 to 8% homology). However, data in Table 1 do indicate a close relations ...
Application title: Cloning genes for expression in AAV vectors for
... biopharmaceuticals (Pollock et al., 1999). But besides the difficulty in generating transgenic founders using current techniques, the time taken to then establish a production herd, approximately 7 years, is prohibitive. The production of recombinant proteins following transduction of target organs ...
... biopharmaceuticals (Pollock et al., 1999). But besides the difficulty in generating transgenic founders using current techniques, the time taken to then establish a production herd, approximately 7 years, is prohibitive. The production of recombinant proteins following transduction of target organs ...
The Maintenance and Propagation of Plasmid Genes in Bacterial
... two obvious possibilities present themselves. Either the plasmid could co-segregate with the chromosome (for example, as part of the nucleoid), or it could actively segregate independently via a membrane attachment site. Multicopy plasmids, in principle, can use an alternative strategy since their c ...
... two obvious possibilities present themselves. Either the plasmid could co-segregate with the chromosome (for example, as part of the nucleoid), or it could actively segregate independently via a membrane attachment site. Multicopy plasmids, in principle, can use an alternative strategy since their c ...
Genomic and Physiological Comparisons Between Heterotrophic
... insight is supported by the homology results shown in Table 1. If the two organisms differ by one or a few mutations, their DNAs should show nearly 100% homology (within the experimental precision of the method used, which is -5 to 8% homology). However, data in Table 1 do indicate a close relations ...
... insight is supported by the homology results shown in Table 1. If the two organisms differ by one or a few mutations, their DNAs should show nearly 100% homology (within the experimental precision of the method used, which is -5 to 8% homology). However, data in Table 1 do indicate a close relations ...
FUNCTION IN PHYSARUM POLYCEPHALUM mitochondria of
... (wt/vol) Triton X-100 (16). The suspension was homogenized for 30 s at 3,000 rpm in a 50-ml Waring blender cup. The foam was removed with a pipette. The homogehate was filtered by gravity through two thicknesses of nylon meshes with pores of 31 #m (NBC Ind., Japan). 10 ml of the homogenate was layer ...
... (wt/vol) Triton X-100 (16). The suspension was homogenized for 30 s at 3,000 rpm in a 50-ml Waring blender cup. The foam was removed with a pipette. The homogehate was filtered by gravity through two thicknesses of nylon meshes with pores of 31 #m (NBC Ind., Japan). 10 ml of the homogenate was layer ...
Parasitoids, predators and PCR: the use of diagnostic
... and quantification of parasitism in a host population generally rely on rearing and dissection of host material. However, there has been increasing interest in the use of molecular methods to identify parasitoids and assess parasitism in host populations. Stouthamer et al. (1999) noted that the advan ...
... and quantification of parasitism in a host population generally rely on rearing and dissection of host material. However, there has been increasing interest in the use of molecular methods to identify parasitoids and assess parasitism in host populations. Stouthamer et al. (1999) noted that the advan ...
Amplification of 16S rRNA Genes from Frankia Strains in Root
... ranged from 0 to 5 CFU on LB agar and R2A agar per 104 hyphal clusters. Each hyphal cluster contained between 102 and 103 Frankia genomes, so the measurable proportion of contaminating bacterial genomes was quite low. Phenolic compounds present in actinorhizal nodule homogenates normally turn bright ...
... ranged from 0 to 5 CFU on LB agar and R2A agar per 104 hyphal clusters. Each hyphal cluster contained between 102 and 103 Frankia genomes, so the measurable proportion of contaminating bacterial genomes was quite low. Phenolic compounds present in actinorhizal nodule homogenates normally turn bright ...
animal genetics
... of Independent Assortment, but otherwise, these principles are recognized as the basis of inheritance. ...
... of Independent Assortment, but otherwise, these principles are recognized as the basis of inheritance. ...
Power Point
... of Independent Assortment, but otherwise, these principles are recognized as the basis of inheritance. ...
... of Independent Assortment, but otherwise, these principles are recognized as the basis of inheritance. ...
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... boundaries can be determined by making deletions that progressively remove more material from one side. When one deletion fails to prevent RNA synthesis but the next stops transcription, the boundary of the promoter must ...
... boundaries can be determined by making deletions that progressively remove more material from one side. When one deletion fails to prevent RNA synthesis but the next stops transcription, the boundary of the promoter must ...
Mitochondrial DNA and aging
... THEORY OF AGING Despite the fact that in animal cells mtDNA comprises only 1–3 % of genetic material, several lines of evidence suggest that its contribution to cellular physiology could be much greater than would be expected from its size alone. For instance, (i) it mutates at higher rates than nuc ...
... THEORY OF AGING Despite the fact that in animal cells mtDNA comprises only 1–3 % of genetic material, several lines of evidence suggest that its contribution to cellular physiology could be much greater than would be expected from its size alone. For instance, (i) it mutates at higher rates than nuc ...
Ammonium transport in Escherichia coli: localization and nucleotide
... DNA ligase, urea, and acrylamide were obtained from BoehringerMannheim. Ampicillin, tetracycline, ammonium acetate, sodium glutamate, bis-acrylamide, boric acid, EDTA and Tris were obtained from Sigma. Agarose was obtained from International Biotechnologies. pBluescript KS( +) vector and the exonucl ...
... DNA ligase, urea, and acrylamide were obtained from BoehringerMannheim. Ampicillin, tetracycline, ammonium acetate, sodium glutamate, bis-acrylamide, boric acid, EDTA and Tris were obtained from Sigma. Agarose was obtained from International Biotechnologies. pBluescript KS( +) vector and the exonucl ...
the association of chloroplast dna with photosynthetic membrane
... Figs. 16 and 17 from a separate experiment. The underlying membranes can be seen more readily in these photographs. Fig. 16 is the type of pattern shown in Figs. 1015 where there are peripheral grana rich regions. Fig. 17 shows a partially swollen chloroplast in which a number of swollen stromal reg ...
... Figs. 16 and 17 from a separate experiment. The underlying membranes can be seen more readily in these photographs. Fig. 16 is the type of pattern shown in Figs. 1015 where there are peripheral grana rich regions. Fig. 17 shows a partially swollen chloroplast in which a number of swollen stromal reg ...
DNA Polyacrylamide Gel Electrophoresis
... 9. Use a Pasteur pipette or a syringe to flush out the wells once more with 1x TBE. Mix the DNA samples with the appropriate amount of gelloading buffer. Load the mixture into the wells using a micropipette equipped with a drawn-out plastic tip. Do not attempt to expel all of the sample from the loa ...
... 9. Use a Pasteur pipette or a syringe to flush out the wells once more with 1x TBE. Mix the DNA samples with the appropriate amount of gelloading buffer. Load the mixture into the wells using a micropipette equipped with a drawn-out plastic tip. Do not attempt to expel all of the sample from the loa ...
Marker assisted selection for crop improvement
... 4. Current obstacles for the adoption of MAS There are many barriers to the adoption of MAS in plant breeding. Currently, one of the most important barriers for MAS in rice today is the prohibitive cost. Although there are only a small number of reports analyzing the economics of MAS versus convent ...
... 4. Current obstacles for the adoption of MAS There are many barriers to the adoption of MAS in plant breeding. Currently, one of the most important barriers for MAS in rice today is the prohibitive cost. Although there are only a small number of reports analyzing the economics of MAS versus convent ...
Molecular evidence for the existence of additional members of the
... Design of primers and probes. The 16s rRNA gene was chosen as the target, since it was the only gene of which the sequence was available for all known members of the Chlamydiaceae. All sequences were downloaded from GenBank and aligned. Possible primer and probe locations compatible with all known C ...
... Design of primers and probes. The 16s rRNA gene was chosen as the target, since it was the only gene of which the sequence was available for all known members of the Chlamydiaceae. All sequences were downloaded from GenBank and aligned. Possible primer and probe locations compatible with all known C ...
Characterizing a Lambda Red Recombinase Induced Presumptive
... The λ Red recombination system was used in this study in an attempt to inactivate the lacI gene in Escherichia coli C29 cells. The proposed model retained the first 41 amino acids of the lacI gene, and replaced the rest of the gene with a linear double-stranded DNA PCR product that confers kanamycin ...
... The λ Red recombination system was used in this study in an attempt to inactivate the lacI gene in Escherichia coli C29 cells. The proposed model retained the first 41 amino acids of the lacI gene, and replaced the rest of the gene with a linear double-stranded DNA PCR product that confers kanamycin ...
pGEX-5X-3 GST Expression Vector
... Purified plasmid will contain predominantly supercoiled form at typically greater than 90% by agarose gel electrophoresis. Chromasomal DNA from the host is not observed. Plasmid is assayed to demonstrate presence of Bam H1; EcoR I; Not I restriction endonuclease sites. ...
... Purified plasmid will contain predominantly supercoiled form at typically greater than 90% by agarose gel electrophoresis. Chromasomal DNA from the host is not observed. Plasmid is assayed to demonstrate presence of Bam H1; EcoR I; Not I restriction endonuclease sites. ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.