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UV-Targeted Dinucleotides Are Not Depleted in Light
... content of intergenic sequences and the XpY content of coding sequences, which is strong evidence for general DNA mechanisms common to both coding and intergenic sequences. This shows that in highly constrained CDS sequences, our method is able to recover general signals also present in intergenic s ...
... content of intergenic sequences and the XpY content of coding sequences, which is strong evidence for general DNA mechanisms common to both coding and intergenic sequences. This shows that in highly constrained CDS sequences, our method is able to recover general signals also present in intergenic s ...
Charge Transport in DNA - Insights from
... of nucleobases on time scales larger than nanoseconds, dramatic enhancements in computational efficiency have to be made. In the final part of this work, one possible approach to this is developed - a parametrized model to simulate the CT in DNA. Not only will this model be able to describe the CT in ...
... of nucleobases on time scales larger than nanoseconds, dramatic enhancements in computational efficiency have to be made. In the final part of this work, one possible approach to this is developed - a parametrized model to simulate the CT in DNA. Not only will this model be able to describe the CT in ...
Intelligent Icons: Integrating Lite-Weight Data Mining
... The three files in the example are ASCII text files, each of which contains approximately 16,000 base pairs of mitochondrial DNA. Here we used string edit distance as suggested in [15] to measure the distance between file names, and Euclidean distance to measure the distance between the file icons ( ...
... The three files in the example are ASCII text files, each of which contains approximately 16,000 base pairs of mitochondrial DNA. Here we used string edit distance as suggested in [15] to measure the distance between file names, and Euclidean distance to measure the distance between the file icons ( ...
PDF
... AtLIG1_3⬘UTR-Rev, 5⬘-CTGGCCTCAGAGCACATTTCCT-3⬘. Primers used to amplify the control GAPDH mRNA were GAPDH3⬘, 5⬘GTAGCCCCACTCGTTGTCGTA-3⬘ and GAPDH5⬘, 5⬘-AGGGTGGTGCCAAGAAGGTTG-3⬘. GAPDH primers were designed to span introns, such that genome-specific and cDNA-specific PCR products could easily be dist ...
... AtLIG1_3⬘UTR-Rev, 5⬘-CTGGCCTCAGAGCACATTTCCT-3⬘. Primers used to amplify the control GAPDH mRNA were GAPDH3⬘, 5⬘GTAGCCCCACTCGTTGTCGTA-3⬘ and GAPDH5⬘, 5⬘-AGGGTGGTGCCAAGAAGGTTG-3⬘. GAPDH primers were designed to span introns, such that genome-specific and cDNA-specific PCR products could easily be dist ...
Comparison of hair and DNA-based approaches in dietary analysis
... Amplification success of prey DNA was significantly higher using the fecal powderQiagen DNeasy kit (50%) compared to the fecal powder-Qiagen stool kit (30%) or the surface mucosal swabbing method (34%) in our initial comparisons (p<0.05 for both comparisons, Student’s t-test, n=28). All subsequent D ...
... Amplification success of prey DNA was significantly higher using the fecal powderQiagen DNeasy kit (50%) compared to the fecal powder-Qiagen stool kit (30%) or the surface mucosal swabbing method (34%) in our initial comparisons (p<0.05 for both comparisons, Student’s t-test, n=28). All subsequent D ...
Lecture-Mic 623-Plasmids-Listeria - Home
... developed for insertional inactivation of chromosomal Listeria monocytogenes genes. ...
... developed for insertional inactivation of chromosomal Listeria monocytogenes genes. ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
... approximately 1200 bp PCR product besides the approximately 1500 bp specific product, making direct sequencing impossible. Sequence analyses gave interesting results. The reported 0.2 % difference between 16S rRNA gene sequences of type strains of R. qingshengii and R. jialingiae was not found, beca ...
... approximately 1200 bp PCR product besides the approximately 1500 bp specific product, making direct sequencing impossible. Sequence analyses gave interesting results. The reported 0.2 % difference between 16S rRNA gene sequences of type strains of R. qingshengii and R. jialingiae was not found, beca ...
Lectures prepared by Christine L. Case Chapter 8 Microbial Genetics
... Competent cells (勝任細胞) can pick up DNA from dead cells and incorporate it into genome by recombination (e.g. antibiotic resistance). Transformed cell than passes genetic recombination to progeny. Transformation works best when donor and recipient are related but they do not have to be. ...
... Competent cells (勝任細胞) can pick up DNA from dead cells and incorporate it into genome by recombination (e.g. antibiotic resistance). Transformed cell than passes genetic recombination to progeny. Transformation works best when donor and recipient are related but they do not have to be. ...
No Evidence of Neandertal mtDNA Contribution to Early Modern
... included samples that have sometimes been considered ‘‘transitional’’ between Neandertals and modern humans, based on their morphological features: Vindija (Smith 1984) and Mladeč (Frayer 1986, 1992; Wolpoff 1999). If low amounts of DNA are preserved in a specimen, some extracts will fail to contai ...
... included samples that have sometimes been considered ‘‘transitional’’ between Neandertals and modern humans, based on their morphological features: Vindija (Smith 1984) and Mladeč (Frayer 1986, 1992; Wolpoff 1999). If low amounts of DNA are preserved in a specimen, some extracts will fail to contai ...
Document
... The amount of DNA that must be loaded depends on the relative abundance of the target sequence to which hybridization will take place. The detection limit of a radioactive probe with a specific activity of 108 to 109 dpm/µg is about 0.5 pg DNA. Thus, for human genomic DNA, 10mg – equivalent to 1.5 p ...
... The amount of DNA that must be loaded depends on the relative abundance of the target sequence to which hybridization will take place. The detection limit of a radioactive probe with a specific activity of 108 to 109 dpm/µg is about 0.5 pg DNA. Thus, for human genomic DNA, 10mg – equivalent to 1.5 p ...
Microbial characterization of white mats in a hydrothermally
... plasmid by competent E. coli cells, and culturing of the E. coli on plates to form colonies. In theory, each colony growing on the agar plate originates from a single replicating E. coli cell, and therefore contains multiple co ...
... plasmid by competent E. coli cells, and culturing of the E. coli on plates to form colonies. In theory, each colony growing on the agar plate originates from a single replicating E. coli cell, and therefore contains multiple co ...
ISEcp1 pneumoniae Research Article
... patient Temoniera. It gives resistance to penicillins and initial cephalosporins [4]. TEM enzymes, resistant to inhibitors have been noticed as a result of mutations [5]. A Sulphydryl variable (SHV) enzyme confers resistance against ampicillin, ticarcillin, cephalosporins and piperacillin [6]. It ma ...
... patient Temoniera. It gives resistance to penicillins and initial cephalosporins [4]. TEM enzymes, resistant to inhibitors have been noticed as a result of mutations [5]. A Sulphydryl variable (SHV) enzyme confers resistance against ampicillin, ticarcillin, cephalosporins and piperacillin [6]. It ma ...
Molecular events during translocation and proofreading extracted
... Structural meta-analysis based on singular value decomposition (SVD) of interatomic distance matrices has enabled a large-scale joint analysis of related protein structures (12–14). This computational method of structural analysis takes advantage of a mathematical, yet practical, definition of confo ...
... Structural meta-analysis based on singular value decomposition (SVD) of interatomic distance matrices has enabled a large-scale joint analysis of related protein structures (12–14). This computational method of structural analysis takes advantage of a mathematical, yet practical, definition of confo ...
Chapter 5
... Data from these experiments are shown in Table 1. He analyzed these data and noticed patterns. For example, from the data of crosses between hybrid plants with purple flowers, he found that the ratio of purple flowers to white flowers was about 3 : 1. This means purple-flowering pea plants grew from ...
... Data from these experiments are shown in Table 1. He analyzed these data and noticed patterns. For example, from the data of crosses between hybrid plants with purple flowers, he found that the ratio of purple flowers to white flowers was about 3 : 1. This means purple-flowering pea plants grew from ...
MA112 Expresso® Rhamnose SUMO Cloning and
... used for both clone construction and protein expression. This single-host strategy allows a streamlined workflow compared to systems requiring separate hosts for cloning and expression. The System uses an engineered form of the SUMO protein (Small Ubiquitin-like MOdifier) as a fusion partner to aid ...
... used for both clone construction and protein expression. This single-host strategy allows a streamlined workflow compared to systems requiring separate hosts for cloning and expression. The System uses an engineered form of the SUMO protein (Small Ubiquitin-like MOdifier) as a fusion partner to aid ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
... approximately 1200 bp PCR product besides the approximately 1500 bp specific product, making direct sequencing impossible. Sequence analyses gave interesting results. The reported 0.2 % difference between 16S rRNA gene sequences of type strains of R. qingshengii and R. jialingiae was not found, beca ...
... approximately 1200 bp PCR product besides the approximately 1500 bp specific product, making direct sequencing impossible. Sequence analyses gave interesting results. The reported 0.2 % difference between 16S rRNA gene sequences of type strains of R. qingshengii and R. jialingiae was not found, beca ...
08_chapter 1
... genomes have no selective advantage in complying with PR2 (Albrecht-Buehler, 2006). According to Albrecht-Buehler (2006), PR2 is not an outcome of the statistical regularity expected in case of long natural sequences. The prime meth.odology used in his work was count statistics of the triplets and t ...
... genomes have no selective advantage in complying with PR2 (Albrecht-Buehler, 2006). According to Albrecht-Buehler (2006), PR2 is not an outcome of the statistical regularity expected in case of long natural sequences. The prime meth.odology used in his work was count statistics of the triplets and t ...
functional analysis of chromatin assembly genes in tetrahymena
... Amplification and molecular cloning of ~1Kb homologous sequences flanking up and ...
... Amplification and molecular cloning of ~1Kb homologous sequences flanking up and ...
Bacterial genome replication at subzero temperatures in permafrost
... CH4, but the studies were performed at higher incubation temperatures ranging from 4 to 25 1C (Martineau et al., 2010; Liebner et al., 2011; He et al., 2012). Here, we used the SIP method to elucidate how specific members of the bacterial community responded to various subzero incubation temperature ...
... CH4, but the studies were performed at higher incubation temperatures ranging from 4 to 25 1C (Martineau et al., 2010; Liebner et al., 2011; He et al., 2012). Here, we used the SIP method to elucidate how specific members of the bacterial community responded to various subzero incubation temperature ...
spectroscopic studies of mosquito iridescent virus, its capsid
... composition of the capsid proteins, the type of a nucleic acid inside the virion (RNA or DNA), and the concentration of virions in the environment or in the tissues of an infected animal. In particular, the fluorescence may be a novel tool to detect viruses and to monitor a viral infection of cells ...
... composition of the capsid proteins, the type of a nucleic acid inside the virion (RNA or DNA), and the concentration of virions in the environment or in the tissues of an infected animal. In particular, the fluorescence may be a novel tool to detect viruses and to monitor a viral infection of cells ...
Transcription is the synthesis of RNA under the direction of DNA
... HIV has an RNA genome that is duplicated into DNA. The resulting DNA can be merged with the DNA genome of the host cell. The main enzyme responsible for synthesis of DNA from an RNA template is called reverse transcriptase. In the case of HIV, reverse transcriptase is responsible for synthesizing a ...
... HIV has an RNA genome that is duplicated into DNA. The resulting DNA can be merged with the DNA genome of the host cell. The main enzyme responsible for synthesis of DNA from an RNA template is called reverse transcriptase. In the case of HIV, reverse transcriptase is responsible for synthesizing a ...
Notes: Biotechnology
... The _________________ world has led to the emerging field of biotechnology which has given us many advances and new _____________ in medicine, agriculture, _______________, and _____________ science. Biotechnology, while it has benefited North Carolina in many ways, has also raised many ________ ...
... The _________________ world has led to the emerging field of biotechnology which has given us many advances and new _____________ in medicine, agriculture, _______________, and _____________ science. Biotechnology, while it has benefited North Carolina in many ways, has also raised many ________ ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.