Gene quantification using real-time quantitative PCR
... of normal individuals in order to determine how background levels of this rearrangement might impact the measurement of disease in patients with FL [43]. Many of these studies have benefited greatly by the application of realtime Q-PCR methods. With highly sensitive and quantitative Q-PCR assays ava ...
... of normal individuals in order to determine how background levels of this rearrangement might impact the measurement of disease in patients with FL [43]. Many of these studies have benefited greatly by the application of realtime Q-PCR methods. With highly sensitive and quantitative Q-PCR assays ava ...
Vibrio diabolicus sp. nov., a New Polysaccharide
... RNase (5 pg/ml) at 60°C for 1 h. This step was followed by one additional extraction with phenol-chloroform-isoamyl alcohol (24:24:1) and one extraction with chloroform. The DNA was precipitated by adding 2.5 volumes of cold pure ethanol, and the pellet was dried and suspended in TE buffer (10 mM Tr ...
... RNase (5 pg/ml) at 60°C for 1 h. This step was followed by one additional extraction with phenol-chloroform-isoamyl alcohol (24:24:1) and one extraction with chloroform. The DNA was precipitated by adding 2.5 volumes of cold pure ethanol, and the pellet was dried and suspended in TE buffer (10 mM Tr ...
Reverse Transcription PCR (RT-PCR): The Molecular
... The HIV proviral DNA contains the major genes common to all non-transducing retroviruses. These genes are gag, pol and env (see Figure 2). HIV also contains five or six other genes that are much smaller. Retroviral transcription is a complex process producing a variety of RNAs. Production of transcri ...
... The HIV proviral DNA contains the major genes common to all non-transducing retroviruses. These genes are gag, pol and env (see Figure 2). HIV also contains five or six other genes that are much smaller. Retroviral transcription is a complex process producing a variety of RNAs. Production of transcri ...
Homologous Recombination Between Episomal Plasmids and Chromosomes in Yeast.
... pseudorevertants resulting from nonsense suppressors. This was accomplished by replica plating the Ura+ colonies onto plates lacking methionine. Clones that grew on these plates, and thus coincidentally became Ura+ and Met+, were presumed to be due to a nonsense suppressor that suppressed both the u ...
... pseudorevertants resulting from nonsense suppressors. This was accomplished by replica plating the Ura+ colonies onto plates lacking methionine. Clones that grew on these plates, and thus coincidentally became Ura+ and Met+, were presumed to be due to a nonsense suppressor that suppressed both the u ...
CYP2B6 NESTED PCR: A GOOD APPROACH FOR PATIENTS ON METHADONE Original Article
... contribute to clinical outcomes in methadone maintenance therapy. The aim of the present study was to develop a consistent and robust assay to identify such genotypic variants. Methods: We randomly selected patients in the Malay population who were addicted to heroin and had already commenced methad ...
... contribute to clinical outcomes in methadone maintenance therapy. The aim of the present study was to develop a consistent and robust assay to identify such genotypic variants. Methods: We randomly selected patients in the Malay population who were addicted to heroin and had already commenced methad ...
Q 4 - Good Laboratory Practice when Performing Molecular
... laboratories in which polymerase chain reactions are performed. This document describes procedures that will help to minimise the carry-over of amplified DNA. Whilst the guidelines concern the majority of PCR applications, they are most relevant where ‘in-house’ assays are in use and may be less rel ...
... laboratories in which polymerase chain reactions are performed. This document describes procedures that will help to minimise the carry-over of amplified DNA. Whilst the guidelines concern the majority of PCR applications, they are most relevant where ‘in-house’ assays are in use and may be less rel ...
Document
... • Single CpG methylation model – distinct DNA elements [usually transcription factor (TF)-binding sites] whose methylation or demethylation usually leads to gene silencing or activation ...
... • Single CpG methylation model – distinct DNA elements [usually transcription factor (TF)-binding sites] whose methylation or demethylation usually leads to gene silencing or activation ...
DNA Sequencing by Targeting 16S rRNA Gene for Novel Strain
... characteristic of any known genus and species. The traditional identification of bacteria on the basis of phenotypic characteristics is generally not as accurate as identification based on genotypic methods 13. Comparison of the bacterial 16S rRNA gene sequence has emerged as a preferred genetic tec ...
... characteristic of any known genus and species. The traditional identification of bacteria on the basis of phenotypic characteristics is generally not as accurate as identification based on genotypic methods 13. Comparison of the bacterial 16S rRNA gene sequence has emerged as a preferred genetic tec ...
Chapter C4
... • Proteins and Traits Proteins act as chemical triggers for many of the processes within cells. Proteins help determine traits. • Help from RNA Another type of molecule that helps make proteins is called RNA, or ribonucleic acid. RNA is so similar to DNA that RNA can serve as a temporary copy of a D ...
... • Proteins and Traits Proteins act as chemical triggers for many of the processes within cells. Proteins help determine traits. • Help from RNA Another type of molecule that helps make proteins is called RNA, or ribonucleic acid. RNA is so similar to DNA that RNA can serve as a temporary copy of a D ...
The coiled-coil of the human Rad50 DNA repair protein contains
... he protein complex containing Rad50 and Mre11 (R兾M) plays a pivotal role in maintaining genome stability. Its vital importance is underscored by its conservation from bacteriophages to humans (1). Based on amino acid sequence similarities, Rad50 belongs to the structural maintenance of chromosomes ( ...
... he protein complex containing Rad50 and Mre11 (R兾M) plays a pivotal role in maintaining genome stability. Its vital importance is underscored by its conservation from bacteriophages to humans (1). Based on amino acid sequence similarities, Rad50 belongs to the structural maintenance of chromosomes ( ...
Chapter 6
... form proteins of many shapes and sizes. The function of a protein depends on the shape of the bonded amino acids. If even a single amino acid is missing or out of place, the protein may not function correctly or may not function. Foods such as meat, fish, cheese, and beans contain proteins, which ar ...
... form proteins of many shapes and sizes. The function of a protein depends on the shape of the bonded amino acids. If even a single amino acid is missing or out of place, the protein may not function correctly or may not function. Foods such as meat, fish, cheese, and beans contain proteins, which ar ...
Analysis of Drosophila Species Genome Size and Satellite DNA
... ABSTRACT The size of eukaryotic genomes can vary by several orders of magnitude, yet genome size does not correlate with the number of genes nor with the size or complexity of the organism. Although ‘‘whole’’-genome sequences, such as those now available for 12 Drosophila species, provide informatio ...
... ABSTRACT The size of eukaryotic genomes can vary by several orders of magnitude, yet genome size does not correlate with the number of genes nor with the size or complexity of the organism. Although ‘‘whole’’-genome sequences, such as those now available for 12 Drosophila species, provide informatio ...
Plants` Epigenetic Secrets
... There are three different types of DNA methylation in plants: CG, CHH (where H is any base except G), and CHG. In Arabidopsis, CG methylation is found on some genes, but primarily on repeat sequences that make up transposons, as well as other repeat sequences in the genome. CHH methylation is found ...
... There are three different types of DNA methylation in plants: CG, CHH (where H is any base except G), and CHG. In Arabidopsis, CG methylation is found on some genes, but primarily on repeat sequences that make up transposons, as well as other repeat sequences in the genome. CHH methylation is found ...
Microbiology
... GRH2 was originally isolated from root nodules of A . yanopbylla (Herrera e t al., 1985). Other bacterial strains used in this work are listed in Table 1. Bacteria were routinely grown in minimal medium (MM; Robertsen e t al., 1981), T Y (Beringer, 1974) or T Y supplemented with mannitol (YMT). Tran ...
... GRH2 was originally isolated from root nodules of A . yanopbylla (Herrera e t al., 1985). Other bacterial strains used in this work are listed in Table 1. Bacteria were routinely grown in minimal medium (MM; Robertsen e t al., 1981), T Y (Beringer, 1974) or T Y supplemented with mannitol (YMT). Tran ...
No Slide Title
... form proteins of many shapes and sizes. The function of a protein depends on the shape of the bonded amino acids. If even a single amino acid is missing or out of place, the protein may not function correctly or may not function. Foods such as meat, fish, cheese, and beans contain proteins, which ar ...
... form proteins of many shapes and sizes. The function of a protein depends on the shape of the bonded amino acids. If even a single amino acid is missing or out of place, the protein may not function correctly or may not function. Foods such as meat, fish, cheese, and beans contain proteins, which ar ...
Oligonucleotide Cross-Linking
... labels of DNA- and RNA-binding proteins, interstrand cross-links between DNA and/or RNA, and capturing any molecule’s approach to DNA or RNA. Cross-linking may be initiated by a variety of chemical reactions, for example, disulfide formation, but the predominant procedure is photo-induced cross-link ...
... labels of DNA- and RNA-binding proteins, interstrand cross-links between DNA and/or RNA, and capturing any molecule’s approach to DNA or RNA. Cross-linking may be initiated by a variety of chemical reactions, for example, disulfide formation, but the predominant procedure is photo-induced cross-link ...
Properties and sequence of the coenzyme B12
... was ampli¢ed by PCR and cloned into pBluescript SK+ in the same orientation as the T7 promoter. The resulting recombinant plasmid designated pLM3 was used to transform E. coli K38/pGP1-2, which contains on the plasmid pGP1-2 bacteriophage T7 RNA polymerase under control of the VpL promoter and the t ...
... was ampli¢ed by PCR and cloned into pBluescript SK+ in the same orientation as the T7 promoter. The resulting recombinant plasmid designated pLM3 was used to transform E. coli K38/pGP1-2, which contains on the plasmid pGP1-2 bacteriophage T7 RNA polymerase under control of the VpL promoter and the t ...
Analysis of Two Genes Encoding Prothrombin Activators in
... Sequence analysis of P. textilis FV and PCNS intron 2 The DNA sequencing results showed that intron 2 of FV is 1,555 bp in length, whilst the PCNS is 1,740 bp. Aside of the presence of two minor short insertions near the 3’ end of PCNS intron 2, there is a 283 bp insertion near the 5’ end of the seq ...
... Sequence analysis of P. textilis FV and PCNS intron 2 The DNA sequencing results showed that intron 2 of FV is 1,555 bp in length, whilst the PCNS is 1,740 bp. Aside of the presence of two minor short insertions near the 3’ end of PCNS intron 2, there is a 283 bp insertion near the 5’ end of the seq ...
Molecular Genetics and Genomics
... probe. These fragments were subcloned in pRK404 to obtain plasmids pBSB43 and pBSB42, respectively. Plasmid pBSB43 complemented R. meliloti 7094, allowing formation of ¯uorescent colonies on Calco¯uor LB agar and eective nodulation of alfalfa, but plasmid pBSB42 did not complement any of the mutant ...
... probe. These fragments were subcloned in pRK404 to obtain plasmids pBSB43 and pBSB42, respectively. Plasmid pBSB43 complemented R. meliloti 7094, allowing formation of ¯uorescent colonies on Calco¯uor LB agar and eective nodulation of alfalfa, but plasmid pBSB42 did not complement any of the mutant ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.