![Isolation and characterization of a repeated sequence (RPS1) of](http://s1.studyres.com/store/data/022477310_1-8787d6b1cdbaf4dabd51c8046daa8feb-300x300.png)
Isolation and characterization of a repeated sequence (RPS1) of
... chromosomes. The karyotypes of the isolates recovered from individual patients after intervals of 1-6 months were virtually identical; however, one or two chromosomes were variable in size (Asakura et al., 1991). In virtually all cases, the chromosome that varied in size was chromosome 2. This sugge ...
... chromosomes. The karyotypes of the isolates recovered from individual patients after intervals of 1-6 months were virtually identical; however, one or two chromosomes were variable in size (Asakura et al., 1991). In virtually all cases, the chromosome that varied in size was chromosome 2. This sugge ...
Isolation of AOXI promoter
... the blood alcohol concentration of an individual. It is constructed using DNA from Pichia pastoris, a strain of yeast with a diauxic metabolic pathway for ethanol and methanol. The alcohol sensor will utilize this metabolic activity, along with a fluorescent protein indicator fused with the alcohol ...
... the blood alcohol concentration of an individual. It is constructed using DNA from Pichia pastoris, a strain of yeast with a diauxic metabolic pathway for ethanol and methanol. The alcohol sensor will utilize this metabolic activity, along with a fluorescent protein indicator fused with the alcohol ...
Towards Programmable Molecular Machines
... Recent related results: Several experimental molecular machines have been recently developed in a laboratory setting. These machines are usually powered either by a natural phenomenon called strand invasion (also known as branch migration) or by enzymes that can break the backbone of DNA at location ...
... Recent related results: Several experimental molecular machines have been recently developed in a laboratory setting. These machines are usually powered either by a natural phenomenon called strand invasion (also known as branch migration) or by enzymes that can break the backbone of DNA at location ...
Diagnostic protocol for
... Aliquots of 25 µl of each bacterial preparation or plant samples to be tested are pipetted onto the windows of a plastic-coated multiwindow microscope slide, allowed to air-dry and gently heat-fixed over a flame. Separate slides are set up for each test bacterium and also, positive and negative cont ...
... Aliquots of 25 µl of each bacterial preparation or plant samples to be tested are pipetted onto the windows of a plastic-coated multiwindow microscope slide, allowed to air-dry and gently heat-fixed over a flame. Separate slides are set up for each test bacterium and also, positive and negative cont ...
7.1 The lac Operon
... The Oc lac operator binds repressor with lower affinity than does the wild-type operator. Cohn and colleagues performed a lac operator–repressor binding assay as described in last figure, using three different DNAs as follows: red, DNA containing a wild type operator (O+); blue, DNA containing an o ...
... The Oc lac operator binds repressor with lower affinity than does the wild-type operator. Cohn and colleagues performed a lac operator–repressor binding assay as described in last figure, using three different DNAs as follows: red, DNA containing a wild type operator (O+); blue, DNA containing an o ...
M.Sc., Biotechnology - Alagappa University
... field of biochemistry and medicine are closely related. Health and disease of living organisms depend on the accustomed balance and abnormalities of biomolecules, biochemical reactions, or biochemical processes occurring in the body. Exploring these biochemical reactions with the recent advancement ...
... field of biochemistry and medicine are closely related. Health and disease of living organisms depend on the accustomed balance and abnormalities of biomolecules, biochemical reactions, or biochemical processes occurring in the body. Exploring these biochemical reactions with the recent advancement ...
Applied Microbiology and Biotechnology
... (Stanley et al. 1983). When M. capsulatus is used for SCP production, pMMO formation is preferred because this enzyme allows a more efficient carbon to biomass conversion than sMMO (Jørgensen and Degn 1987). Besides methane, both sMMO and pMMO oxidise several other substrates (Colby and Dalton 1978; ...
... (Stanley et al. 1983). When M. capsulatus is used for SCP production, pMMO formation is preferred because this enzyme allows a more efficient carbon to biomass conversion than sMMO (Jørgensen and Degn 1987). Besides methane, both sMMO and pMMO oxidise several other substrates (Colby and Dalton 1978; ...
Multiplex RT-PCR kit.
... Limit of detection is 10-8 g of fusion RNA in a sample of 1 g total RNA when the RNA quality is good. This test brings IVD testing deeper into a detailed description of the exon organization of fusion genes originating from chromosome translocations. This information is important for predicting de ...
... Limit of detection is 10-8 g of fusion RNA in a sample of 1 g total RNA when the RNA quality is good. This test brings IVD testing deeper into a detailed description of the exon organization of fusion genes originating from chromosome translocations. This information is important for predicting de ...
Thermodynamic analysis of DNA binding by a Bacillus single
... Results: We have cloned and purified SSB from Bacillus anthracis (SSBBA). In the absence of DNA, at concentrations ≤100 μg/ml, SSBBA did not form a stable tetramer and appeared to resemble bacteriophage T4 gene 32 protein. Fluorescence anisotropy studies demonstrated that SSBBA bound ssDNA with high ...
... Results: We have cloned and purified SSB from Bacillus anthracis (SSBBA). In the absence of DNA, at concentrations ≤100 μg/ml, SSBBA did not form a stable tetramer and appeared to resemble bacteriophage T4 gene 32 protein. Fluorescence anisotropy studies demonstrated that SSBBA bound ssDNA with high ...
Lecture Notes for Methods in Cell Biology
... acids and recombinant DNA. The first section will cover some basic biochemical procedures and equipment. Understanding these basic biochemical principals will assist in the subsequent discussions on proteins and nucleic acids. The section on characterization of proteins will describe some basic meth ...
... acids and recombinant DNA. The first section will cover some basic biochemical procedures and equipment. Understanding these basic biochemical principals will assist in the subsequent discussions on proteins and nucleic acids. The section on characterization of proteins will describe some basic meth ...
"PCR-based Species Identification and Gene Tagging"
... maize leaf _Bots infected leaf pt3 infected leaf pt1 P sorghi pt3 P sorghi pt1 Marker ...
... maize leaf _Bots infected leaf pt3 infected leaf pt1 P sorghi pt3 P sorghi pt1 Marker ...
Drosophila - The Solomon H Snyder Department of Neuroscience
... replication and a drug resistance marker, which is then isolated after transforming into bacteria for sequence analysis. The iPCR method is sufficient to map the insertion site for most transposable elements, and has been used in high-throughput screens to isolate the insertion sites for thousands o ...
... replication and a drug resistance marker, which is then isolated after transforming into bacteria for sequence analysis. The iPCR method is sufficient to map the insertion site for most transposable elements, and has been used in high-throughput screens to isolate the insertion sites for thousands o ...
Deoxyribonucleic Acid Base Compositions and Nucleotide
... Rhodomicrobium, and genus D. In cases where detergent and enzyme treatments failed, the cells had to be disintegrated mechanically by using glass beads and the minimum time necessary for destroying approximately 50% of the cells. In this way, only one-half of the cell material was actually exploited ...
... Rhodomicrobium, and genus D. In cases where detergent and enzyme treatments failed, the cells had to be disintegrated mechanically by using glass beads and the minimum time necessary for destroying approximately 50% of the cells. In this way, only one-half of the cell material was actually exploited ...
Mechanisms of fast and stringent search in homologous pairing of
... publish, or preparation of the manuscript. ...
... publish, or preparation of the manuscript. ...
Sequence Information Encoded in DNA that May Influence Long
... cell type may have different chromatin structures from those $300 kb regions that do produce transcripts. This size should be large enough to form relatively stable higher-order chromatin structures, if such structures exist. We flagged only those 300 kb regions that were annotated to have no gene o ...
... cell type may have different chromatin structures from those $300 kb regions that do produce transcripts. This size should be large enough to form relatively stable higher-order chromatin structures, if such structures exist. We flagged only those 300 kb regions that were annotated to have no gene o ...
Grade 12 - Curriculum
... The following Grade 10, 11 and 12 Lesson Plans were developed by Subject Advisors during May 2009. Teachers are requested to look at them, modify them where necessary to suit their contexts and resources. It must be remembered that Lesson Plans are working documents, and any comments to improve the ...
... The following Grade 10, 11 and 12 Lesson Plans were developed by Subject Advisors during May 2009. Teachers are requested to look at them, modify them where necessary to suit their contexts and resources. It must be remembered that Lesson Plans are working documents, and any comments to improve the ...
Abiogenesis
... – RNA is very similar to DNA – We still have RNA in our body cells – It can catalyse its own reactions – RNA is a plausible precursor to DNA © Colin Frayn, 2008 www.frayn.net ...
... – RNA is very similar to DNA – We still have RNA in our body cells – It can catalyse its own reactions – RNA is a plausible precursor to DNA © Colin Frayn, 2008 www.frayn.net ...
The Arabidopsis RAD51 paralogs RAD51B, RAD51D and XRCC2
... Our results provide strong evidence that Arabidopsis XRCC2, RAD51B and RAD51D have complex functions in somatic DNA repair and gene regulation, arguing for further studies of these ancient genes that have been maintained in both plants and animals during their long evolutionary history. ...
... Our results provide strong evidence that Arabidopsis XRCC2, RAD51B and RAD51D have complex functions in somatic DNA repair and gene regulation, arguing for further studies of these ancient genes that have been maintained in both plants and animals during their long evolutionary history. ...
Mechanisms of Unidirectional Translocation & Unwinding
... Stepping requires at least 2 NA binding sites that independently bind and release NA and change the distance between each other. • Monomeric helicases: 2 hands represent 2 parts of NA binding site that move relative to each other. Both sites are controlled by a single NTPase site. • Oligomeric helic ...
... Stepping requires at least 2 NA binding sites that independently bind and release NA and change the distance between each other. • Monomeric helicases: 2 hands represent 2 parts of NA binding site that move relative to each other. Both sites are controlled by a single NTPase site. • Oligomeric helic ...
NIH Public Access
... primary DNA methyltransferase that maintains cytosine methylation at CG sites [14]. met1 mutants display a global reduction of cytosine methylation accompanied with developmental abnormalities [15]. From genetic studies, it was shown that imprinting of MEA, FIS2, and the FWA transcription factor gen ...
... primary DNA methyltransferase that maintains cytosine methylation at CG sites [14]. met1 mutants display a global reduction of cytosine methylation accompanied with developmental abnormalities [15]. From genetic studies, it was shown that imprinting of MEA, FIS2, and the FWA transcription factor gen ...
Spring 1997 - University of Idaho
... DNA-FeII ,- CuI complexes participates in Fenton type chemistries. DNA degradation by .OH (or other oxidizing products) leads to attack on deoxyribose residues which releases bases from strands. ...
... DNA-FeII ,- CuI complexes participates in Fenton type chemistries. DNA degradation by .OH (or other oxidizing products) leads to attack on deoxyribose residues which releases bases from strands. ...
Products > HT-1080 Transfection Reagent
... Transfection Reagent (Fibrosarcoma Cells) HT-1080 Transfection Kit Benefits • Pre-optimized transfection protocol for HT-1080 cell line • Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing • Free of serum and protein of animal origin • Compatible with standard a ...
... Transfection Reagent (Fibrosarcoma Cells) HT-1080 Transfection Kit Benefits • Pre-optimized transfection protocol for HT-1080 cell line • Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing • Free of serum and protein of animal origin • Compatible with standard a ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.