Chicken eggshell matrix proteins related to anti
... Eggshell matrix proteins related to antibacterial proteins: J. Gautron et al. eggshell mineralization occurs. These previously identified matrix proteins can be divided into three groups according to their characteristics (Table 1). 1. The most abundant egg white proteins have been shown to be loca ...
... Eggshell matrix proteins related to antibacterial proteins: J. Gautron et al. eggshell mineralization occurs. These previously identified matrix proteins can be divided into three groups according to their characteristics (Table 1). 1. The most abundant egg white proteins have been shown to be loca ...
Electrophoresis
... is used to identify and positionally match all spots on the different gels. • The intensities are then compared for the Cy3 and Cy5 values of the different spots, and statistics done to see which ones have significantly changed in intensity as a consequence of the experimental treatment. ...
... is used to identify and positionally match all spots on the different gels. • The intensities are then compared for the Cy3 and Cy5 values of the different spots, and statistics done to see which ones have significantly changed in intensity as a consequence of the experimental treatment. ...
Low-temperature anaerobic digestion is associated with differential
... methane from both CO2 and acetate (see corresponding proteins in Table 1) at the time of sampling. Interestingly, a subunit of methyl-coenzyme M reductase, catalyzing the last step of methanogenesis, assigned to Methanoculleus marisnigri (Methanomicrobiales) showed reduced levels of expression as th ...
... methane from both CO2 and acetate (see corresponding proteins in Table 1) at the time of sampling. Interestingly, a subunit of methyl-coenzyme M reductase, catalyzing the last step of methanogenesis, assigned to Methanoculleus marisnigri (Methanomicrobiales) showed reduced levels of expression as th ...
mbe.oxfordjournals.org - Oxford Academic
... biogenesis, nucleolar assembly, and regulation of vital cellular processes. However, this nucleolar inventory has not unveiled a specific consensus motif necessary for nucleolar binding. The ribosomal protein family characterized by their basic nature should exhibit distinct binding sequences that e ...
... biogenesis, nucleolar assembly, and regulation of vital cellular processes. However, this nucleolar inventory has not unveiled a specific consensus motif necessary for nucleolar binding. The ribosomal protein family characterized by their basic nature should exhibit distinct binding sequences that e ...
Crk: The First Identified Adaptor Protein
... polyclonal antibody. Significant levels of Crk were isolated in carcinoma of lung, breast, and stomach, as well as intrapelvic tumors In control tissues, Crk was detected in ependymal cell layer in brain, bronchial epithelium of lung, and bile duct epithelium of liver. Crk was only detected in norma ...
... polyclonal antibody. Significant levels of Crk were isolated in carcinoma of lung, breast, and stomach, as well as intrapelvic tumors In control tissues, Crk was detected in ependymal cell layer in brain, bronchial epithelium of lung, and bile duct epithelium of liver. Crk was only detected in norma ...
COMBINATION COMPRISING AT LEAST ONE AMINO ACID AND A
... [0014] AKT is a serine/threonine kinase, also known as protein kinase B (PKB). Activation of AKT occurs through direct binding of the inositol lipid products of the PI3K to its pleckstrin homology domain. PI3K-dependent activation of AKT also occurs through phosphoinositide-dependent kinase (PDK1)-m ...
... [0014] AKT is a serine/threonine kinase, also known as protein kinase B (PKB). Activation of AKT occurs through direct binding of the inositol lipid products of the PI3K to its pleckstrin homology domain. PI3K-dependent activation of AKT also occurs through phosphoinositide-dependent kinase (PDK1)-m ...
AMIN domains have a predicted role in localization of diverse
... on protein assemblies that are restricted to specific subcellular locations (Gitai, 2005). Perhaps the most striking example of this is the cell polarization of rod-shaped bacteria. In addition to being the cellular site of structures such as polar flagella and pilli, several proteins have been rece ...
... on protein assemblies that are restricted to specific subcellular locations (Gitai, 2005). Perhaps the most striking example of this is the cell polarization of rod-shaped bacteria. In addition to being the cellular site of structures such as polar flagella and pilli, several proteins have been rece ...
Prediction of mitochondrial proteins of malaria parasite
... acid composition. We developed a SVM model using amino acid composition and got maximum MCC 0.38 with accuracy of 74.14%. Similarly, SVM models were developed using dipeptide composition which achieved maximum MCC 0.50 with accuracy of 82.76%. In past it has been shown that composition of parts of a ...
... acid composition. We developed a SVM model using amino acid composition and got maximum MCC 0.38 with accuracy of 74.14%. Similarly, SVM models were developed using dipeptide composition which achieved maximum MCC 0.50 with accuracy of 82.76%. In past it has been shown that composition of parts of a ...
Dynamics of Protein Tyrosine Nitration and
... cardiovascular injury and cancer. It even facilitates in the accumulation of nitrated proteins in vivo [11]. The most common neurodegenerative diseases are Alzheimer's disease, Huntington's disease and Parkinson's disease etc. These Diseases are also referred to as proteinopathies due to the aggrega ...
... cardiovascular injury and cancer. It even facilitates in the accumulation of nitrated proteins in vivo [11]. The most common neurodegenerative diseases are Alzheimer's disease, Huntington's disease and Parkinson's disease etc. These Diseases are also referred to as proteinopathies due to the aggrega ...
This is the accepted version of the following article:
... cerebrospinal fluid (CSF), but not in plasma or serum, have been generally accepted as sensitive AD diagnostic markers [5]. Since phosphorylation may change some physico-chemical properties and functions of protein [6, 7] it is necessary to operate under in vitro studies also with the natural struct ...
... cerebrospinal fluid (CSF), but not in plasma or serum, have been generally accepted as sensitive AD diagnostic markers [5]. Since phosphorylation may change some physico-chemical properties and functions of protein [6, 7] it is necessary to operate under in vitro studies also with the natural struct ...
144803525 - BORA
... Tyrosine hydroxylase (TH) is the rate limiting enzyme that catalyzes the first step in the biosynthesis of catecholamines. The posttranslational modification of its regulatory domain controls the regulation of TH enzyme activity. Different kinases are responsible for the phosphorylation of the enzym ...
... Tyrosine hydroxylase (TH) is the rate limiting enzyme that catalyzes the first step in the biosynthesis of catecholamines. The posttranslational modification of its regulatory domain controls the regulation of TH enzyme activity. Different kinases are responsible for the phosphorylation of the enzym ...
Circadian Rhythm of Intestinal SucraseActivity in Rats
... animals were sacrificed at appropriate intervals for study of solubilized sucrase-isomaltase as described below. Enzyme solubilization. The following procedures were done at 4°C. The animals were stunned, decapitated, and the abdominal cavity opened with prompt removal of the small intestine from th ...
... animals were sacrificed at appropriate intervals for study of solubilized sucrase-isomaltase as described below. Enzyme solubilization. The following procedures were done at 4°C. The animals were stunned, decapitated, and the abdominal cavity opened with prompt removal of the small intestine from th ...
STRUCTURE AND FUNCTION
... and versatile signaling proteins. PII proteins are small trimeric signal transduction proteins that regulate gene transcription, modulate activity of regulatory proteins, and control the catalytic activity of nitrogen metabolism. Two PII proteins in E.coli are GlnB and GlnK and they can bind ATP, 2- ...
... and versatile signaling proteins. PII proteins are small trimeric signal transduction proteins that regulate gene transcription, modulate activity of regulatory proteins, and control the catalytic activity of nitrogen metabolism. Two PII proteins in E.coli are GlnB and GlnK and they can bind ATP, 2- ...
Efficiency assay of detergent removal columns on - G
... OFFGEL electrophoresis and SDS‐PAGE: 100 μg samples were separated on 4‐7 pH strip, 12 fractions using the 3100 OFFGEL fractionator (Agilent). After applying 20 kV, fractions were collected and 1/3 was dried down and run via SDS‐PAGE. The gel was stained with Sypro. In solution digestion: Samples we ...
... OFFGEL electrophoresis and SDS‐PAGE: 100 μg samples were separated on 4‐7 pH strip, 12 fractions using the 3100 OFFGEL fractionator (Agilent). After applying 20 kV, fractions were collected and 1/3 was dried down and run via SDS‐PAGE. The gel was stained with Sypro. In solution digestion: Samples we ...
supp - Springer Static Content Server
... many cases, it was demonstrated that two RRMs separated by short linkers bind RNA ...
... many cases, it was demonstrated that two RRMs separated by short linkers bind RNA ...
The Calcium-Binding Activity of a Vacuole
... VCaB45 protein levels might be correlated with the presence of vacuoles, we exploited the characteristic of celery petioles that allows, with relative ease, the physical separation of vascular tissues from cortical tissue. The parenchyma cells in the cortical tissues tend to be extensively vacuolate ...
... VCaB45 protein levels might be correlated with the presence of vacuoles, we exploited the characteristic of celery petioles that allows, with relative ease, the physical separation of vascular tissues from cortical tissue. The parenchyma cells in the cortical tissues tend to be extensively vacuolate ...
Conservation and relative importance of residues across protein
... rim or the rest of the protein surface. With the division into core and rim residues one can ask the question of whether as two groups these residues have different contributions to binding energy, and consequently have different evolutionary pressure for their conservation. The degree of conservati ...
... rim or the rest of the protein surface. With the division into core and rim residues one can ask the question of whether as two groups these residues have different contributions to binding energy, and consequently have different evolutionary pressure for their conservation. The degree of conservati ...
Protein translocation channel of mitochondrial inner
... growth of yeast cells, whereas no viable colonies were obtained when an empty plasmid was used, confirming the specificity of the assay. We conclude that the N-terminal domain of Tim44, even when extended to include the membrane-recruitment helices of the C-terminal domain, is not sufficient to supp ...
... growth of yeast cells, whereas no viable colonies were obtained when an empty plasmid was used, confirming the specificity of the assay. We conclude that the N-terminal domain of Tim44, even when extended to include the membrane-recruitment helices of the C-terminal domain, is not sufficient to supp ...
The Proteasomes
... A very interesting feature of proteasomes is their role in the immune system. Proteasomes can syntheisize short peptide fragments that are then used as antigens in lymphocytes. These antigens are presented on the surface of these cells (through the MHC complex) and play an important role in the cell ...
... A very interesting feature of proteasomes is their role in the immune system. Proteasomes can syntheisize short peptide fragments that are then used as antigens in lymphocytes. These antigens are presented on the surface of these cells (through the MHC complex) and play an important role in the cell ...
Novel Multiprotein Complexes Identified in the Hyperthermophilic
... false positives (16). The epitope tag affinity purification and tandem affinity purification methods have also been used extensively (17–21), but the tags can disrupt native proteinprotein interactions, and the methods tend to be biased toward proteins that interact with high affinity and/or protein ...
... false positives (16). The epitope tag affinity purification and tandem affinity purification methods have also been used extensively (17–21), but the tags can disrupt native proteinprotein interactions, and the methods tend to be biased toward proteins that interact with high affinity and/or protein ...
Classifier ensembles for protein structural class prediction with
... prediction by using alternative sequence representations. Examples include auto-correlation functions based on non-bonded residue energy [7], polypeptide composition [26,37,44], and functional domain composition [16]. However, these algorithms are often only tested on very small datasets, with uncon ...
... prediction by using alternative sequence representations. Examples include auto-correlation functions based on non-bonded residue energy [7], polypeptide composition [26,37,44], and functional domain composition [16]. However, these algorithms are often only tested on very small datasets, with uncon ...
A Major Surface Protein on Group A Streptococci Is a
... as important constituents of human saliva (17, 18). The surfaces of both Gram-negative and Gram-positive bacteria have been shown to contain molecules that bind to fibronectin and lysozyme (19-21). While a fibronectin binding protein in Staphylococcus aureus has been identified (22), characterized ( ...
... as important constituents of human saliva (17, 18). The surfaces of both Gram-negative and Gram-positive bacteria have been shown to contain molecules that bind to fibronectin and lysozyme (19-21). While a fibronectin binding protein in Staphylococcus aureus has been identified (22), characterized ( ...
Protein for Athletes
... muscle is more than just protein; it also contains water, fat, glycogen, and some minerals. One pound of muscle contains 70 to 105 g of protein, and to build a pound of muscle, it is estimated that 10 to 14 g of additional protein is needed each day,2 although others dispute this claim. In addition ...
... muscle is more than just protein; it also contains water, fat, glycogen, and some minerals. One pound of muscle contains 70 to 105 g of protein, and to build a pound of muscle, it is estimated that 10 to 14 g of additional protein is needed each day,2 although others dispute this claim. In addition ...
Box 1 - Open Biology
... MCPH (16). WDR62 is located at chromosomal position 19q13.12, is 4,746 bp in length and consists of 32 exons encoding a 166 kDa protein. The protein contains a conserved region of WD40 repeat domains (17). These are found in many eukaryotic proteins and are involved in numerous cellular functions in ...
... MCPH (16). WDR62 is located at chromosomal position 19q13.12, is 4,746 bp in length and consists of 32 exons encoding a 166 kDa protein. The protein contains a conserved region of WD40 repeat domains (17). These are found in many eukaryotic proteins and are involved in numerous cellular functions in ...
Dietary protein for athletes - Inside Outside Wellness Center
... lower than that seen with lower-quality proteins. In addition, the intensity of the exercise performed by the subjects in studies in which protein requirements were elevated (Friedman and Lemon 1989; Lemon et al. 1992; Meredith et al. 1989; Tarnopolsky et al. 1988, 1992) was greater than that in stu ...
... lower than that seen with lower-quality proteins. In addition, the intensity of the exercise performed by the subjects in studies in which protein requirements were elevated (Friedman and Lemon 1989; Lemon et al. 1992; Meredith et al. 1989; Tarnopolsky et al. 1988, 1992) was greater than that in stu ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.