Nugget
... The E. coli SSB protein is a helix-destabilizing protein that binds with high affinity to ssDNA intermediates during replication, recombination and DNA repair. It has been proposed that SSB, while bound to ssDNA, can translocate along the ssDNA via a rolling mechansim. The protein cannot translocate ...
... The E. coli SSB protein is a helix-destabilizing protein that binds with high affinity to ssDNA intermediates during replication, recombination and DNA repair. It has been proposed that SSB, while bound to ssDNA, can translocate along the ssDNA via a rolling mechansim. The protein cannot translocate ...
This presentation introduces the topics we will
... multiple entries, the numbers may not work. If the protein sequence, or something very similar, is not in the database, the method will fail. If you are studying a well characterised organism, such as human or mouse or yeast, this is unlikely to be a problem. If you are studying a virus or plant wit ...
... multiple entries, the numbers may not work. If the protein sequence, or something very similar, is not in the database, the method will fail. If you are studying a well characterised organism, such as human or mouse or yeast, this is unlikely to be a problem. If you are studying a virus or plant wit ...
Nucleotide sequences of genome segments $8, encoding a capsid
... 47419 (47K). Among the RGDV proteins, the 45K protein, the major constituent of the outer capsid (Omura et al., 1985), was the closest in size to the predicted polypeptide. Hence, partial amino acid sequences of the outer capsid protein were analysed and compared with those of the predicted 47K poly ...
... 47419 (47K). Among the RGDV proteins, the 45K protein, the major constituent of the outer capsid (Omura et al., 1985), was the closest in size to the predicted polypeptide. Hence, partial amino acid sequences of the outer capsid protein were analysed and compared with those of the predicted 47K poly ...
Nucleotide sequences of genome segments $8, encoding a capsid
... 47419 (47K). Among the RGDV proteins, the 45K protein, the major constituent of the outer capsid (Omura et al., 1985), was the closest in size to the predicted polypeptide. Hence, partial amino acid sequences of the outer capsid protein were analysed and compared with those of the predicted 47K poly ...
... 47419 (47K). Among the RGDV proteins, the 45K protein, the major constituent of the outer capsid (Omura et al., 1985), was the closest in size to the predicted polypeptide. Hence, partial amino acid sequences of the outer capsid protein were analysed and compared with those of the predicted 47K poly ...
the simple quad method - New Moon Family Acupuncture
... Carbohydrate, and a small amount of Fat. Your serving size for the entire meal should be about the size of your two hands cupped together. This is the amount that your stomach can comfortably handle at each meal. An example would be baked chicken, cooked carrots, salad and a small baked potato. Or g ...
... Carbohydrate, and a small amount of Fat. Your serving size for the entire meal should be about the size of your two hands cupped together. This is the amount that your stomach can comfortably handle at each meal. An example would be baked chicken, cooked carrots, salad and a small baked potato. Or g ...
Gene Section BRD4 (bromodomain containing 4) Atlas of Genetics and Cytogenetics
... The BRD4-NUT fusion protein is composed of the Nterminal of BRD4 (amino acids 1-720 out of 1372) and almost the entire protein sequence of NUT (amino acids 6-1127). The N-terminal of BRD4 includes bromodomains 1 and 2 and other, less well ...
... The BRD4-NUT fusion protein is composed of the Nterminal of BRD4 (amino acids 1-720 out of 1372) and almost the entire protein sequence of NUT (amino acids 6-1127). The N-terminal of BRD4 includes bromodomains 1 and 2 and other, less well ...
Mitochondrial stress causes increased succination of proteins in
... Nutrient excess has been documented to result in mitochondrial damage in the expanding adipocyte [11,12], suggesting that an increase in mitochondrial stress occurs as a result of fuel excess [13,14]. Intra-adipocyte organelle stress [15,16] may culminate in adipocyte hypertrophy and cell death, in ...
... Nutrient excess has been documented to result in mitochondrial damage in the expanding adipocyte [11,12], suggesting that an increase in mitochondrial stress occurs as a result of fuel excess [13,14]. Intra-adipocyte organelle stress [15,16] may culminate in adipocyte hypertrophy and cell death, in ...
The 92-kDa chitinase from Streptomyces olivaceoviridis contains a
... During the incubation period, increasing protease activity became visible at the 42-, 22- and 14-kDa protein bands, but not at the 70- and 30-kDa protein bands. ...
... During the incubation period, increasing protease activity became visible at the 42-, 22- and 14-kDa protein bands, but not at the 70- and 30-kDa protein bands. ...
Bioinformatics of proteins: Sequence, structure and the `symbiosis
... Protein 3D Structures A protein’s structure has a critical effect on its function: 2. Areas of specific chemical\electrical properties ...
... Protein 3D Structures A protein’s structure has a critical effect on its function: 2. Areas of specific chemical\electrical properties ...
Post-Transcriptional Regulation of Gene Expression: the Many Hats
... structures of their complexes. Such information will define better the principles of recognition of RNA elements by proteins. We have focussed here on hnRNP A2, but this protein has two paralogues, hnRNP A1 and A3, and we have evidence that the latter is also involved in RNA transport. It will be in ...
... structures of their complexes. Such information will define better the principles of recognition of RNA elements by proteins. We have focussed here on hnRNP A2, but this protein has two paralogues, hnRNP A1 and A3, and we have evidence that the latter is also involved in RNA transport. It will be in ...
Determination of Protein Molecular Weight
... Determination of Protein Molecular Weight Proteins are a highly diversified class of biomolecules. Differences in their chemical properties, such as charge, functional groups, shape, size and solubility enable them to perform many biological functions. These functions include enzyme catalysis, metab ...
... Determination of Protein Molecular Weight Proteins are a highly diversified class of biomolecules. Differences in their chemical properties, such as charge, functional groups, shape, size and solubility enable them to perform many biological functions. These functions include enzyme catalysis, metab ...
S11. Computational Molecular Modeling- Week 5. 3
... a course management system. In the advanced instructions, students generate protein sequence alignments and upload them into ICM-Browser. Once uploaded into ICM-Browser, the instructor and students determine what regions of the 3-D protein models to analyze, based on the sequence alignment. Compare ...
... a course management system. In the advanced instructions, students generate protein sequence alignments and upload them into ICM-Browser. Once uploaded into ICM-Browser, the instructor and students determine what regions of the 3-D protein models to analyze, based on the sequence alignment. Compare ...
Electrophoretic Extraction and Proteomic Characterization of
... of particulate nitrogen in marine sediments [5–8]. In addition to proteins representing the largest fraction of organic nitrogen, within the unique amino acid sequence, proteins also can provide functional and phylogenetic information on the organisms from which they were produced, potentially makin ...
... of particulate nitrogen in marine sediments [5–8]. In addition to proteins representing the largest fraction of organic nitrogen, within the unique amino acid sequence, proteins also can provide functional and phylogenetic information on the organisms from which they were produced, potentially makin ...
Health relevance of intestinal protein fermentation in young
... than their isoelectric point (Cosgrove, 1966). The protein molecules become closely packed around the phytate anion forming insoluble aggregates (Rajendran and Prakash, 1993) that are refractory to pepsin digestion (Knuckles et al., 1989). At a pH above their isoelectric point, proteins carrying a n ...
... than their isoelectric point (Cosgrove, 1966). The protein molecules become closely packed around the phytate anion forming insoluble aggregates (Rajendran and Prakash, 1993) that are refractory to pepsin digestion (Knuckles et al., 1989). At a pH above their isoelectric point, proteins carrying a n ...
Protein Kinase A Regulatory Subunit Interacts with P
... proteins with high affinity, mediating the compartmentalization of PKA and ensuring its specificity by placing PKAc close to its appropriate effectors and substrates.4 In T. cruzi, the PKAr binding proteins remain unknown. We identified several P-type ATPases that interact with PKAr. We also tested ...
... proteins with high affinity, mediating the compartmentalization of PKA and ensuring its specificity by placing PKAc close to its appropriate effectors and substrates.4 In T. cruzi, the PKAr binding proteins remain unknown. We identified several P-type ATPases that interact with PKAr. We also tested ...
Structural and Thermal Stability Characterization of Protein
... Table.2.3.Showing the solubility of C18SA modified hemoglobin in different organic solvents.200ul of 50uM C18SA modified BSA was mixed with 1mL of each organic solvent to test the solubility ...
... Table.2.3.Showing the solubility of C18SA modified hemoglobin in different organic solvents.200ul of 50uM C18SA modified BSA was mixed with 1mL of each organic solvent to test the solubility ...
Planta
... into the vector pDONR207 through a BP reaction (Invitrogen Gateway® Technology). The resulting entry clone was used for recombination with plant and yeast expression vectors through LR reactions. In fusions of fluorescent proteins to the C terminus of VvCTr1, the stop codon was omitted in the revers ...
... into the vector pDONR207 through a BP reaction (Invitrogen Gateway® Technology). The resulting entry clone was used for recombination with plant and yeast expression vectors through LR reactions. In fusions of fluorescent proteins to the C terminus of VvCTr1, the stop codon was omitted in the revers ...
Histoplasma capsulatum an effective mechanism for trans-cell wall transfer
... Yeast cells secrete a calcium-binding protein that enables the fungus to grow in calcium-limiting conditions (Sebghati et al., 2000). Heat-shock proteins are also produced at a high level, which is consistent with the thermally dimorphic nature of the organism (Burnie et al., ...
... Yeast cells secrete a calcium-binding protein that enables the fungus to grow in calcium-limiting conditions (Sebghati et al., 2000). Heat-shock proteins are also produced at a high level, which is consistent with the thermally dimorphic nature of the organism (Burnie et al., ...
Peroxisomes - University of California San Diego
... only in yeast. It is an intraperoxisomal organizer of the peroxisomal import machinery in S. cerevisiae. A 44 kDa integral PMP found only in the yeast Yarrowia lipolytica. A ,35 kDa integral PMP with a carboxy-terminal, cytoplasmically-exposed, zinc binding RING domain. Has been identified in yeasts ...
... only in yeast. It is an intraperoxisomal organizer of the peroxisomal import machinery in S. cerevisiae. A 44 kDa integral PMP found only in the yeast Yarrowia lipolytica. A ,35 kDa integral PMP with a carboxy-terminal, cytoplasmically-exposed, zinc binding RING domain. Has been identified in yeasts ...
Protein reutilisation in corms of Colchicum autumnale
... mother corm. The regular bud gives rise to new daughter corm and its shoot regularly at the end of August. The new shoot (yet without roots) is in flower in the middle of September. After flowering the differentiation of roots and the complete future above-ground part (stem, leaves and capsules) takes ...
... mother corm. The regular bud gives rise to new daughter corm and its shoot regularly at the end of August. The new shoot (yet without roots) is in flower in the middle of September. After flowering the differentiation of roots and the complete future above-ground part (stem, leaves and capsules) takes ...
Animal Dicer and plant Dicer-like proteins
... et al., 2012a). This offers significant potential to influence the nuclease activity of the enzyme; yet the functions of both domains appear highly diverse. The helicase domain has been shown to have an inhibitory effect on dsRNA processing by human Dicer (Ma et al., 2008). This domain is also cruci ...
... et al., 2012a). This offers significant potential to influence the nuclease activity of the enzyme; yet the functions of both domains appear highly diverse. The helicase domain has been shown to have an inhibitory effect on dsRNA processing by human Dicer (Ma et al., 2008). This domain is also cruci ...
Identification of a novel viral protein in infectious bursal disease
... purified IPNV. However, no correlation of these products with ORF A-2 has so far been shown, although it has been suggested that this protein is probably virusspecific. Comparison between the amino acid sequences of ORF A-2 proteins of different strains of IBDV and IPNV has indicated only limited am ...
... purified IPNV. However, no correlation of these products with ORF A-2 has so far been shown, although it has been suggested that this protein is probably virusspecific. Comparison between the amino acid sequences of ORF A-2 proteins of different strains of IBDV and IPNV has indicated only limited am ...
Protein Composition of a High-Protein Barley Flour and Barley Grain
... (Konzak 1977, Lasztity 1984). This is similar to the results of this study for the partly dehulled barley grain. The distribution of the proteins among the different fractions, according to their solubility, is also known to depend on the variety, cultivation condition (Ldsztity 1984), and the fract ...
... (Konzak 1977, Lasztity 1984). This is similar to the results of this study for the partly dehulled barley grain. The distribution of the proteins among the different fractions, according to their solubility, is also known to depend on the variety, cultivation condition (Ldsztity 1984), and the fract ...
Primary Structure of Diphtheria Toxin Fragment B
... 1), positively charged residues are at the periphery (residues Arg 17, Lys 19, 21, 23, and 28, and His 30) and negatively charged residues at the center (residues Asp 18, Glu 25 and 29), forming ion pairs or triplets. The helical segment 47-67 of ...
... 1), positively charged residues are at the periphery (residues Arg 17, Lys 19, 21, 23, and 28, and His 30) and negatively charged residues at the center (residues Asp 18, Glu 25 and 29), forming ion pairs or triplets. The helical segment 47-67 of ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.