Document
... Providing high quality animal-derived proteins for human nutrition is an essential role of animal production. Amino acids are the building blocks of protein,which are composed of an amino group (-NH2), a carboxyl group (-COOH) and a side chain specific for each AA. Proteins are polymers of AA where ...
... Providing high quality animal-derived proteins for human nutrition is an essential role of animal production. Amino acids are the building blocks of protein,which are composed of an amino group (-NH2), a carboxyl group (-COOH) and a side chain specific for each AA. Proteins are polymers of AA where ...
Di-(2-ethylhexyl) phthalate mediates glycolysis and the TCA cycle
... response in marine invertebrates. In this study, GS-MS was employed to determine the bio-accumulated levels of DEHP in clam Venerupis philippinarum. After exposure to 0.4 mg L-1 and 4mg L-1 DEHP, the bio-accumulated DEHP in the clam foot was significantly increased in the first 24 h, and then sharpl ...
... response in marine invertebrates. In this study, GS-MS was employed to determine the bio-accumulated levels of DEHP in clam Venerupis philippinarum. After exposure to 0.4 mg L-1 and 4mg L-1 DEHP, the bio-accumulated DEHP in the clam foot was significantly increased in the first 24 h, and then sharpl ...
ERNDIM - Quantitative Schemes Cystine in White Blood Cells
... to this the SAB has agreed levels of adequate performance for all the schemes and these will be re-evaluated annually. The scoring systems have been carefully evaluated by members of the SAB and have been applied to assess performance in our schemes from 2007 onwards. The ERNDIM Board has decided t ...
... to this the SAB has agreed levels of adequate performance for all the schemes and these will be re-evaluated annually. The scoring systems have been carefully evaluated by members of the SAB and have been applied to assess performance in our schemes from 2007 onwards. The ERNDIM Board has decided t ...
Localization of Protein-Protein lnteractions between Subunits of
... We have used the h repressor (cl) as the first component in our assay system. The native h repressor is a homodimer of 26-kD (236 residues) subunits. The protein is divided into two domains: an amino-terminal, DNA binding domain (residues 1 to 92) and a carboxy-terminal domain (residues 132to 236), ...
... We have used the h repressor (cl) as the first component in our assay system. The native h repressor is a homodimer of 26-kD (236 residues) subunits. The protein is divided into two domains: an amino-terminal, DNA binding domain (residues 1 to 92) and a carboxy-terminal domain (residues 132to 236), ...
Localization of Protein-Protein lnteractions between Subunits of
... We have used the h repressor (cl) as the first component in our assay system. The native h repressor is a homodimer of 26-kD (236 residues) subunits. The protein is divided into two domains: an amino-terminal, DNA binding domain (residues 1 to 92) and a carboxy-terminal domain (residues 132to 236), ...
... We have used the h repressor (cl) as the first component in our assay system. The native h repressor is a homodimer of 26-kD (236 residues) subunits. The protein is divided into two domains: an amino-terminal, DNA binding domain (residues 1 to 92) and a carboxy-terminal domain (residues 132to 236), ...
"Fluorescence Spectrophotometry". In: Encyclopedia of Life Science
... The measurement of fluorescence signals provides a sensitive method of monitoring the biochemical environment of a fluorophore. Instruments have been designed to measure fluorescence intensity, spectrum, lifetime and polarization. Fluorescence intensity measurement allows the determination of the prese ...
... The measurement of fluorescence signals provides a sensitive method of monitoring the biochemical environment of a fluorophore. Instruments have been designed to measure fluorescence intensity, spectrum, lifetime and polarization. Fluorescence intensity measurement allows the determination of the prese ...
Protein splicing of PRP8 mini-inteins from species of the genus
... Analysis of protein splicing of Penicillium PRP8 inteins To determine whether the prp8 inteins of P. chrysogenum, P. expansum, and P. vulpinum are functional, we tested their protein-splicing activity in E. coli cells. Plasmid-borne fusion genes were constructed to produce proteins in which the Peni ...
... Analysis of protein splicing of Penicillium PRP8 inteins To determine whether the prp8 inteins of P. chrysogenum, P. expansum, and P. vulpinum are functional, we tested their protein-splicing activity in E. coli cells. Plasmid-borne fusion genes were constructed to produce proteins in which the Peni ...
State of the Art Manufacturing of Protein Hydrolysates
... is evaporated, pasteurized and spray dried. Enzymatic hydrolysis of proteins: Most of the enzymes used to make protein hydrolysates for applications in biotechnology are obtained from animal, plant and microbial sources. Recent advances in techniques for the hydrolysis of proteins have come from stu ...
... is evaporated, pasteurized and spray dried. Enzymatic hydrolysis of proteins: Most of the enzymes used to make protein hydrolysates for applications in biotechnology are obtained from animal, plant and microbial sources. Recent advances in techniques for the hydrolysis of proteins have come from stu ...
recombinant protein characterization
... While small-molecule drugs are typically produced by chemical synthesis and have welldefined chemical structures, therapeutic proteins pose a much more difficult production and characterization challenge. These proteins can be isolated from a variety of sources, including human, animal, or microorgani ...
... While small-molecule drugs are typically produced by chemical synthesis and have welldefined chemical structures, therapeutic proteins pose a much more difficult production and characterization challenge. These proteins can be isolated from a variety of sources, including human, animal, or microorgani ...
iclicker - University of Colorado-MCDB
... How pluripotency of a stem cell is maintained by a general repressor ...
... How pluripotency of a stem cell is maintained by a general repressor ...
anatomy of a feed tag - Westway Feed Products
... Acid Detergent Fiber is more than 6%, it probably contains an added high fiber ingredient. The fiber added to dry feeds is inexpensive, and is used as a filler. It also has very little nutritional value because it is from sources which have had the useful constituents refined out. Cottonseed hulls, ...
... Acid Detergent Fiber is more than 6%, it probably contains an added high fiber ingredient. The fiber added to dry feeds is inexpensive, and is used as a filler. It also has very little nutritional value because it is from sources which have had the useful constituents refined out. Cottonseed hulls, ...
Divalent Metal Ions in Plant Mitochondria and Their Role in
... plethora of other poorly characterized consequences (Stadtman, 1990). It has been proposed that MCO of proteins can be a highly specific event where proteins are more susceptible to damage if they bind metal ions and when the site of protein oxidation can be defined on the protein surface that binds ...
... plethora of other poorly characterized consequences (Stadtman, 1990). It has been proposed that MCO of proteins can be a highly specific event where proteins are more susceptible to damage if they bind metal ions and when the site of protein oxidation can be defined on the protein surface that binds ...
The transcriptional regulator CprK detects chlorination by combining
... allow the molecule to report on a wide range of cAMP concentrations, as both the half and fully occupied dimers (each with distinct DNA-binding affinities) are populated to significant levels [20]. By contrast, it has been proposed that CprK exhibits either extreme positive or no cooperativity, both ...
... allow the molecule to report on a wide range of cAMP concentrations, as both the half and fully occupied dimers (each with distinct DNA-binding affinities) are populated to significant levels [20]. By contrast, it has been proposed that CprK exhibits either extreme positive or no cooperativity, both ...
application of recombinant smr-domain containing protein of
... Abstract. The aim of this study was to find novel proteins expressed from an Angiostrongylus cantonensis adult female worm cDNA library for serodiagnosis of angiostrongyliasis. An immuno-dominant clone, fAC22, was identified by immunoscreening with pooled positive sera from proven angiostrongyliasis ...
... Abstract. The aim of this study was to find novel proteins expressed from an Angiostrongylus cantonensis adult female worm cDNA library for serodiagnosis of angiostrongyliasis. An immuno-dominant clone, fAC22, was identified by immunoscreening with pooled positive sera from proven angiostrongyliasis ...
Heinrichs, V., and Baker, B. S.
... nucleus enriched in splicing factors (24). The function of the Gly-rich region is unknown. The identification of the functional domains in SR proteins necessary for the regulation of alternative splicing can help to understand which molecular interactions are required in splicing regulation. Both bi ...
... nucleus enriched in splicing factors (24). The function of the Gly-rich region is unknown. The identification of the functional domains in SR proteins necessary for the regulation of alternative splicing can help to understand which molecular interactions are required in splicing regulation. Both bi ...
Function and specificity of 14-3-3 proteins in the regulation of
... led to activation of nitrate reductase and accumulation of ZMP in the tissue without changing the general phosphate metabolism (Huber and Kaiser, 1996). Using AICAR feeding prior to analysing SPS activity, Toroser et al. (1998) showed a reduced 14-3-3 binding to SPS leading to higher SPS activity in ...
... led to activation of nitrate reductase and accumulation of ZMP in the tissue without changing the general phosphate metabolism (Huber and Kaiser, 1996). Using AICAR feeding prior to analysing SPS activity, Toroser et al. (1998) showed a reduced 14-3-3 binding to SPS leading to higher SPS activity in ...
Synthetic human prion protein octapeptide repeat binds to the
... disordered N-terminal tail [7]. The helical domain represents a highly ordered compact structure and probably is less susceptible to proteolysis, at least in the beginning of the hydrolytic attack, than the tail. The N-terminal domain of the prion molecule becomes more structured after binding coppe ...
... disordered N-terminal tail [7]. The helical domain represents a highly ordered compact structure and probably is less susceptible to proteolysis, at least in the beginning of the hydrolytic attack, than the tail. The N-terminal domain of the prion molecule becomes more structured after binding coppe ...
PDGF receptor β and leukemia
... Carroll M, Tomasson MH, Barker GF, Golub TR, and Gilliland DG (1996) The TEL/plateletderived growth factor beta receptor (PDGF beta R) fusion in chronic myelomonocytic leukemia is a transforming protein that self-associates and activates PDGF beta Leukemia R kinase-dependent signaling pathways. Proc ...
... Carroll M, Tomasson MH, Barker GF, Golub TR, and Gilliland DG (1996) The TEL/plateletderived growth factor beta receptor (PDGF beta R) fusion in chronic myelomonocytic leukemia is a transforming protein that self-associates and activates PDGF beta Leukemia R kinase-dependent signaling pathways. Proc ...
Studying Protein Flexibility in a Statistical Framework: Tools and
... (iv) 3814 automatically found conformational change outliers. Wilson et al.45 did a comprehensive set of structural alignments on version 1.39 of the scop database, which represented most the known structures as of the beginning of 1999. From this they found 4403 pairs of domains that had appreciabl ...
... (iv) 3814 automatically found conformational change outliers. Wilson et al.45 did a comprehensive set of structural alignments on version 1.39 of the scop database, which represented most the known structures as of the beginning of 1999. From this they found 4403 pairs of domains that had appreciabl ...
Branched-Chain Amino Acids in Exercise
... sclerosis complex (TSC1 and TSC2), Rheb, and AMP kinase (AMPK) (27–29) (Fig. 3). TSC1/TSC2 and Rheb are crucial regulators situated between protein kinase B (PKB) and mTOR. Rheb, a Ras-like GTPase, is a positive regulator of mTOR in vivo. The action of Rheb is opposed by the TSC1/TSC2 complex, which ...
... sclerosis complex (TSC1 and TSC2), Rheb, and AMP kinase (AMPK) (27–29) (Fig. 3). TSC1/TSC2 and Rheb are crucial regulators situated between protein kinase B (PKB) and mTOR. Rheb, a Ras-like GTPase, is a positive regulator of mTOR in vivo. The action of Rheb is opposed by the TSC1/TSC2 complex, which ...
Branched-Chain Amino Acid Supplementation: A Critical Analysis of
... behind supplementing with free form amino acids vs. traditional dietary sources is it allows for better control over intake, and proportions due to concise measurement; its purported increased digestion, and absorption rates which allow for amino acids to reach targeted tissues sooner (Negro, Giardi ...
... behind supplementing with free form amino acids vs. traditional dietary sources is it allows for better control over intake, and proportions due to concise measurement; its purported increased digestion, and absorption rates which allow for amino acids to reach targeted tissues sooner (Negro, Giardi ...
Journal of Agricultural and Food Chemistry
... mass between 45 and 50 kDa. Electrophoretic patterns of albumin-2 fractions showed that the main protein subunits were concentrated in (kDa): 34.2 for amaranth and 36.4 for soybean. The electrophoretic separation of these plants showed similarity. Variations in protein separation of amaranth and soy ...
... mass between 45 and 50 kDa. Electrophoretic patterns of albumin-2 fractions showed that the main protein subunits were concentrated in (kDa): 34.2 for amaranth and 36.4 for soybean. The electrophoretic separation of these plants showed similarity. Variations in protein separation of amaranth and soy ...
Norton J Nutr 2006
... mTOR are mediated through TSC2. AMPK directly phosphorylates TSC2, increasing formation of the TSC1/TSC2 complex and inhibiting Rheb. Because AMPK is activated by exercise and a decreased ATP/AMP ratio, AMPK activation and its effects on the mTOR pathway appear to be a primary mechanism reducing pro ...
... mTOR are mediated through TSC2. AMPK directly phosphorylates TSC2, increasing formation of the TSC1/TSC2 complex and inhibiting Rheb. Because AMPK is activated by exercise and a decreased ATP/AMP ratio, AMPK activation and its effects on the mTOR pathway appear to be a primary mechanism reducing pro ...
TARGET: a new method for predicting protein subcellular
... protein targeting or signal peptides that are recognized by locationspecific transport machinery to enable their entry (Nielsen et al., 1997; Nakai and Horton, 1999; Emanuelsson et al., 2000). Among these, PSORT is a popular method (Nakai and Horton, 1999) that could predict proteins targeted to 12 ...
... protein targeting or signal peptides that are recognized by locationspecific transport machinery to enable their entry (Nielsen et al., 1997; Nakai and Horton, 1999; Emanuelsson et al., 2000). Among these, PSORT is a popular method (Nakai and Horton, 1999) that could predict proteins targeted to 12 ...
Diseases of the Immune System lec.4
... Amyloidosis results from abnormal folding of proteins, which are deposited as fibrils in extracellular tissues and disrupt normal function. Normally, misfolded proteins are degraded intracellularly in proteasomes, or extracellularly by macrophages. It appears that in amyloidosis, these quality contr ...
... Amyloidosis results from abnormal folding of proteins, which are deposited as fibrils in extracellular tissues and disrupt normal function. Normally, misfolded proteins are degraded intracellularly in proteasomes, or extracellularly by macrophages. It appears that in amyloidosis, these quality contr ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.