Primary production of protein: I. Comparison of net cellular carbon
... synthesis and incorporation of I4C-carbon lnto individual protein amino acid residues revealed that precursor sources of protein-bound glycine and alanine rapidly attained isotopic equhbrium with the I4C-labeled inorganic carbon pool for all growth conditions and in all species examined. Precursor e ...
... synthesis and incorporation of I4C-carbon lnto individual protein amino acid residues revealed that precursor sources of protein-bound glycine and alanine rapidly attained isotopic equhbrium with the I4C-labeled inorganic carbon pool for all growth conditions and in all species examined. Precursor e ...
C urrent and prospective applications of metal ion–protein
... 2. Immobilized metal ion affinity chromatography: utilization of differences in protein–metal ion affinity ......................................... 2.1. Principles and procedures ........................................................................................................................ ...
... 2. Immobilized metal ion affinity chromatography: utilization of differences in protein–metal ion affinity ......................................... 2.1. Principles and procedures ........................................................................................................................ ...
An Engineered Aryl Azide Ligase for Site-Specific
... which is considerably greater than the Km value of wild-type lipoic acid for LplA (1.7 mm [21] or 4.5 mm [24]). We were concerned that remodeling of the LplA active site might change the peptide specificity of the enzyme. Therefore, we characterized the specificity of W37V-catalyzed aryl azide ligat ...
... which is considerably greater than the Km value of wild-type lipoic acid for LplA (1.7 mm [21] or 4.5 mm [24]). We were concerned that remodeling of the LplA active site might change the peptide specificity of the enzyme. Therefore, we characterized the specificity of W37V-catalyzed aryl azide ligat ...
Protein structure
... chemical reactions in the laboratory. In contrast, synthetic (anabolic) and degradative (catabolic) processes, along with the host of specific, high-affinity interactions necessary for life, must occur in a largely aqueous environment and within a rather narrow range of temperatures. To a great exte ...
... chemical reactions in the laboratory. In contrast, synthetic (anabolic) and degradative (catabolic) processes, along with the host of specific, high-affinity interactions necessary for life, must occur in a largely aqueous environment and within a rather narrow range of temperatures. To a great exte ...
BIOCHEMISTRY LAB CHE-554 First portion: A chromophorogenic
... What potential hazards are associated with working with guanidinium HCl ? Does this material contain HCl ? Look at the MSDS for guanidinium HCl available on the course web site and assess this material’s toxicity by comparing its LD50 with those of glucose and aspiring, which are also posted. ...
... What potential hazards are associated with working with guanidinium HCl ? Does this material contain HCl ? Look at the MSDS for guanidinium HCl available on the course web site and assess this material’s toxicity by comparing its LD50 with those of glucose and aspiring, which are also posted. ...
PRIONS THE INFECTIOUS PROTEINS
... What are Calpains? They generate a C-terminal fragment(C2) which has molecular weight of 27-30 kDs. ...
... What are Calpains? They generate a C-terminal fragment(C2) which has molecular weight of 27-30 kDs. ...
![[7] Semisynthesis of Proteins Containing Selenocysteine](http://s1.studyres.com/store/data/004768810_1-d08ecd7536246bbf8b4baa16bb630c93-300x300.png)
[7] Semisynthesis of Proteins Containing Selenocysteine
... to selenocysteine by activation with phenylmethylsulfonyl fluoride (pMSF) and reaction with hydrogen selenide.1 This approach is not general, however, as it relies on the especially high reactivity of the active-site serine residue in subtilisin. A semisynthetic approach for incorporating selenocyst ...
... to selenocysteine by activation with phenylmethylsulfonyl fluoride (pMSF) and reaction with hydrogen selenide.1 This approach is not general, however, as it relies on the especially high reactivity of the active-site serine residue in subtilisin. A semisynthetic approach for incorporating selenocyst ...
EXPRESSION OF IQ-MOTIF GENES IN HUMAN CELLS AND ASPM
... expression was greatly reduced at birth, and that by postnatal day 9 expression was limited only to discrete cells scattered within the neocortex.6 This finding would support our inability to detect ASPM in adult brain tissue. cDNA was prepared from different regions of adult mouse brain, but ASPM w ...
... expression was greatly reduced at birth, and that by postnatal day 9 expression was limited only to discrete cells scattered within the neocortex.6 This finding would support our inability to detect ASPM in adult brain tissue. cDNA was prepared from different regions of adult mouse brain, but ASPM w ...
Polypeptide Composition of Envelopes of Spinach Chloroplasts
... branes which were not fully separated from inner envelope membranes. Thirty amino acid residues of the N-terminus of the 15 kDa protein were determined (Fig. 3A). Homology search of the protein against a DNA database revealed that it is homologous to the 16 kDa protein of outer envelopes (OEP16) fro ...
... branes which were not fully separated from inner envelope membranes. Thirty amino acid residues of the N-terminus of the 15 kDa protein were determined (Fig. 3A). Homology search of the protein against a DNA database revealed that it is homologous to the 16 kDa protein of outer envelopes (OEP16) fro ...
Structural Basis of Biological Nitrogen Fixation
... As a constituent of nearly all biomolecules, nitrogen is essential for life. Although there is an enormous reservoir of dinitrogen in the atmosphere, as described above, kinetically N2 is not reactive toward either oxidation or reduction, and most organisms are unable to directly metabolize this ele ...
... As a constituent of nearly all biomolecules, nitrogen is essential for life. Although there is an enormous reservoir of dinitrogen in the atmosphere, as described above, kinetically N2 is not reactive toward either oxidation or reduction, and most organisms are unable to directly metabolize this ele ...
HIGHLY VISCOUS DOUGH FORMING PROPERTIES OF MARAMA PROTEIN
... defined as the ratio of G" over G', reflects the balance between the viscous and the elastic character of a viscoelastic material (Mezger, 2006). As small dynamic deformation analysis is non-destructive, it can provide some information on the types of molecular bonding that may be responsible for th ...
... defined as the ratio of G" over G', reflects the balance between the viscous and the elastic character of a viscoelastic material (Mezger, 2006). As small dynamic deformation analysis is non-destructive, it can provide some information on the types of molecular bonding that may be responsible for th ...
Understanding Tools and Techniques in Protein Structure Prediction
... structure of collagen molecule while glycosylation, carboxylation and methylation have little or no effects but which alter the chemical properties of the protein. Another important aspect in a protein fold is the activation of inactive proteins by small molecules such as cofactors, which are essent ...
... structure of collagen molecule while glycosylation, carboxylation and methylation have little or no effects but which alter the chemical properties of the protein. Another important aspect in a protein fold is the activation of inactive proteins by small molecules such as cofactors, which are essent ...
Case for support – Programme of work
... Fig 2. (A) Comparison of AtNAP7 with Erwinia SufC, G. theta sulfate ABC protein, and N. punctiforme ABC protein. (B) Localization of AtNAP7/YFP in tobacco stomata. (C) TLC showing that AtNAP7 is an ATPase. (D) AtNAP7 can complement SufC deficiency in E. coli. PMS was added to WT E. coli (MG1655), a ...
... Fig 2. (A) Comparison of AtNAP7 with Erwinia SufC, G. theta sulfate ABC protein, and N. punctiforme ABC protein. (B) Localization of AtNAP7/YFP in tobacco stomata. (C) TLC showing that AtNAP7 is an ATPase. (D) AtNAP7 can complement SufC deficiency in E. coli. PMS was added to WT E. coli (MG1655), a ...
Analysis of a ribose transport operon from Bacillus
... these systems have a protein homologous to the periplasmic substrate-binding protein, even though this bacterium does not have a periplasm. Nevertheless, Perego e t al. (1991) have demonstrated that the periplasmic oligopeptide binding protein OppA is cell wall associated in exponentially growing ce ...
... these systems have a protein homologous to the periplasmic substrate-binding protein, even though this bacterium does not have a periplasm. Nevertheless, Perego e t al. (1991) have demonstrated that the periplasmic oligopeptide binding protein OppA is cell wall associated in exponentially growing ce ...
Different packing of external residues can explain differences in the
... stabilizing forces (Berezovsky and Shakhnovich, 2005; Berezovsky et al., 2005; Fukuchi and Nishikawa, 2001; Liang et al., 2005; Robinson-Rechavi et al., 2006; Zeldovich et al., 2007). Two physical mechanisms for the increase of protein thermostability are suggested (Berezovsky and Shakhnovich, 2005) ...
... stabilizing forces (Berezovsky and Shakhnovich, 2005; Berezovsky et al., 2005; Fukuchi and Nishikawa, 2001; Liang et al., 2005; Robinson-Rechavi et al., 2006; Zeldovich et al., 2007). Two physical mechanisms for the increase of protein thermostability are suggested (Berezovsky and Shakhnovich, 2005) ...
characterization of proteins from the cytoskeleton of giardia lamblia
... proteins might exert an effect in a number of ways: by direct structural interactions with tubulin; by selecting appropriate subunits from among tubulin species; or, after incorporation of common tubulin precursors, by allowing chemical modification of dimers in organelle- or site-specific patterns. ...
... proteins might exert an effect in a number of ways: by direct structural interactions with tubulin; by selecting appropriate subunits from among tubulin species; or, after incorporation of common tubulin precursors, by allowing chemical modification of dimers in organelle- or site-specific patterns. ...
Dairy 17 - Milk Urea Nitrogen
... Changes in milk urea concentration are generally associated with changes in the diet of the dairy cow. For example, low urea contents are associated with low amounts of dietary protein and/ or degradable protein, for example, low D-value mature grass or silage or diets containing large quantities of ...
... Changes in milk urea concentration are generally associated with changes in the diet of the dairy cow. For example, low urea contents are associated with low amounts of dietary protein and/ or degradable protein, for example, low D-value mature grass or silage or diets containing large quantities of ...
Bryan 2012 Research Day Poster
... • Xanthine and Xanthine Oxidase are positive controls for superoxide production. • Microvesicles at both the 2 and 7 hour time points do not show the ability to produce superoxide. ...
... • Xanthine and Xanthine Oxidase are positive controls for superoxide production. • Microvesicles at both the 2 and 7 hour time points do not show the ability to produce superoxide. ...
¹⁵N Heteronuclear Single Quantum Coherence
... hemoglobin α- and β-subunits are too distant to be covalently linked by a short (i.e., <8 residues) peptide (Shaanan, 1983). To generate a single-chain hemoglobin we have chosen to relocate the β-subunit termini by circular permutation, to allow for novel covalent connections between subunits using ...
... hemoglobin α- and β-subunits are too distant to be covalently linked by a short (i.e., <8 residues) peptide (Shaanan, 1983). To generate a single-chain hemoglobin we have chosen to relocate the β-subunit termini by circular permutation, to allow for novel covalent connections between subunits using ...
Steven`s project - The University of Texas at Dallas
... A need exists for the ability to compute the complex workings of amino acid chains. These proteins have been found to fold in many different ways, and the forces that affect these changes have yet to be fully understood. The folded state of a protein is the state in which it finally gains its functi ...
... A need exists for the ability to compute the complex workings of amino acid chains. These proteins have been found to fold in many different ways, and the forces that affect these changes have yet to be fully understood. The folded state of a protein is the state in which it finally gains its functi ...
Types and effects of protein variations. Vihinen
... large proportion appears in protein coding DNA and RNA sequences, although the proteincoding regions constitute only about 1.3 % of human genome. Due to the large number of different functions in which proteins are involved, also the effects of variants are widely different. In addition to functions ...
... large proportion appears in protein coding DNA and RNA sequences, although the proteincoding regions constitute only about 1.3 % of human genome. Due to the large number of different functions in which proteins are involved, also the effects of variants are widely different. In addition to functions ...
Biochemical Evidence for the Role of the Waxy Protein fron Pea
... peak was also studied. The properties of the two isoforms were very similar with respect to their stimulation by citrate and primer preference. Activity in the absence of added primer as a percentage of the activity with primer was higher for GBSSII, suggesting that the latter may contain more endog ...
... peak was also studied. The properties of the two isoforms were very similar with respect to their stimulation by citrate and primer preference. Activity in the absence of added primer as a percentage of the activity with primer was higher for GBSSII, suggesting that the latter may contain more endog ...
Capabilities and limitations of gel electrophoresis for elemental
... in Fig. 1. To ensure this was not an artefact stemming from the sample preparation, the samples were submitted to ultrafiltration. As expected, the main V fraction was detected in the solution containing transferrin, and not as an unbound, low-molecular-mass species. The complex can be further stabi ...
... in Fig. 1. To ensure this was not an artefact stemming from the sample preparation, the samples were submitted to ultrafiltration. As expected, the main V fraction was detected in the solution containing transferrin, and not as an unbound, low-molecular-mass species. The complex can be further stabi ...
Feeney_ku_0099D_12934_DATA_1 - KU ScholarWorks
... Purpose: Oxidative post-translational modification of protein-bound tyrosine residues can have a significant impact on protein structure and function and thus may be important to physiological and pathological processes. Oxidative stress has been correlated with biological aging and many disease sta ...
... Purpose: Oxidative post-translational modification of protein-bound tyrosine residues can have a significant impact on protein structure and function and thus may be important to physiological and pathological processes. Oxidative stress has been correlated with biological aging and many disease sta ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.