Ube2W conjugates ubiquitin to α-amino groups of protein N
... The covalent attachment of the protein ubiquitin to intracellular proteins by a process known as ubiquitylation regulates almost all major cellular systems, predominantly by regulating protein turnover. Ubiquitylation requires the co-ordinated action of three enzymes termed E1, E2 and E3, and typica ...
... The covalent attachment of the protein ubiquitin to intracellular proteins by a process known as ubiquitylation regulates almost all major cellular systems, predominantly by regulating protein turnover. Ubiquitylation requires the co-ordinated action of three enzymes termed E1, E2 and E3, and typica ...
Population Biology of the First Replicators: On
... At this prereplicator stage in the evolution of life there is no life history, since the birth and death processes are intimately coupled through the physical chemistry of a single reaction. With the emergence of nonenzymatic, template-directed replication, the birth and death processes could diverg ...
... At this prereplicator stage in the evolution of life there is no life history, since the birth and death processes are intimately coupled through the physical chemistry of a single reaction. With the emergence of nonenzymatic, template-directed replication, the birth and death processes could diverg ...
Biology - Kenyon College
... radical inactivated by oxygen in vitro (73), but recent evidence suggests that in vivo Pfl is both expressed (2) and functional (19) in the presence of some oxygen. Pfl activity is reversible, so it could convert formate to pyruvate, consuming acetyl-CoA (40, 42). E. coli also has several poorly cha ...
... radical inactivated by oxygen in vitro (73), but recent evidence suggests that in vivo Pfl is both expressed (2) and functional (19) in the presence of some oxygen. Pfl activity is reversible, so it could convert formate to pyruvate, consuming acetyl-CoA (40, 42). E. coli also has several poorly cha ...
De Novo Pyrimidine Nucleotide Synthesis Mainly
... to the Nucleobase:Cation Symporter1 (NCS1) family of transport proteins, also known as purine-related transporters (Saier et al., 2009; www.tcdb.org). The closest Arabidopsis homolog to this protein is encoded by At5g03555. The deduced amino acid sequence exhibits 23% identity and 43% similarity to ...
... to the Nucleobase:Cation Symporter1 (NCS1) family of transport proteins, also known as purine-related transporters (Saier et al., 2009; www.tcdb.org). The closest Arabidopsis homolog to this protein is encoded by At5g03555. The deduced amino acid sequence exhibits 23% identity and 43% similarity to ...
De Novo Pyrimidine Nucleotide Synthesis Mainly
... to the Nucleobase:Cation Symporter1 (NCS1) family of transport proteins, also known as purine-related transporters (Saier et al., 2009; www.tcdb.org). The closest Arabidopsis homolog to this protein is encoded by At5g03555. The deduced amino acid sequence exhibits 23% identity and 43% similarity to ...
... to the Nucleobase:Cation Symporter1 (NCS1) family of transport proteins, also known as purine-related transporters (Saier et al., 2009; www.tcdb.org). The closest Arabidopsis homolog to this protein is encoded by At5g03555. The deduced amino acid sequence exhibits 23% identity and 43% similarity to ...
Gene Section ABL (v-abl Abelson murine leukemia viral oncogene homolog 1)
... shuttling activity, driven by 3 nuclear localisation signals (NLS) and a single nuclear export signal (NES) close to the C-terminal region. Recent data suggest that nuclear and cytoplasmic ABL may have different functions. 1-Nuclear c-ABL plays a major role in the regulation of cell death after DNA ...
... shuttling activity, driven by 3 nuclear localisation signals (NLS) and a single nuclear export signal (NES) close to the C-terminal region. Recent data suggest that nuclear and cytoplasmic ABL may have different functions. 1-Nuclear c-ABL plays a major role in the regulation of cell death after DNA ...
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... AmazeinTM is the trade name of a highly functional protein produced from raw whole corn (maize) by Prairie Gold Inc. The protein is “zein” (commonly pronounced “zayne” to rhyme with rain or cane), which is one of four different classes of proteins in corn. Zein refers to a family of alcohol-soluble ...
... AmazeinTM is the trade name of a highly functional protein produced from raw whole corn (maize) by Prairie Gold Inc. The protein is “zein” (commonly pronounced “zayne” to rhyme with rain or cane), which is one of four different classes of proteins in corn. Zein refers to a family of alcohol-soluble ...
The Interaction between Dietary Protein and Bone Health
... increasing quartiles of intake of animal protein as follows: 1.00 (reference), 0.59 (95% CI: 0.26, 1.34), 0.63 (0.28, 1.42), and 0.31 (0.10, 0.93); P for trend = 0.037. There was a non-significant trend for higher vegetable protein intake to increase fracture risk. In the Adventist Health Study 2, t ...
... increasing quartiles of intake of animal protein as follows: 1.00 (reference), 0.59 (95% CI: 0.26, 1.34), 0.63 (0.28, 1.42), and 0.31 (0.10, 0.93); P for trend = 0.037. There was a non-significant trend for higher vegetable protein intake to increase fracture risk. In the Adventist Health Study 2, t ...
Gene Section FA1 (Fanconi anaemia 1) Atlas of Genetics and Cytogenetics
... Lo Ten Foe JR, Rooimans MA, Bosnoyan-Collins L, Alon N, Wijker M, Parker L, Lightfoot J, Carreau M, Callen DF, Savoia A, Cheng NC, van Berkel CG, Strunk MH, Gille JJ, Pals G, Kruyt FA, Pronk JC, Arwert F, Buchwald M, Joenje H. Expression cloning of a cDNA for the major Fanconi anaemia gene, FAA. Nat ...
... Lo Ten Foe JR, Rooimans MA, Bosnoyan-Collins L, Alon N, Wijker M, Parker L, Lightfoot J, Carreau M, Callen DF, Savoia A, Cheng NC, van Berkel CG, Strunk MH, Gille JJ, Pals G, Kruyt FA, Pronk JC, Arwert F, Buchwald M, Joenje H. Expression cloning of a cDNA for the major Fanconi anaemia gene, FAA. Nat ...
HRP - WordPress.com
... Overall, we improved visibility of our bands in the SDS-PAGE. There are stronger bands indicating that using the concentrator to remove ammonium sulfate and increase HRP concentrations worked. We were also able to extract active HRP within these sequence purification steps. We were able to continue ...
... Overall, we improved visibility of our bands in the SDS-PAGE. There are stronger bands indicating that using the concentrator to remove ammonium sulfate and increase HRP concentrations worked. We were also able to extract active HRP within these sequence purification steps. We were able to continue ...
Polyubiquitination in diseases: implications in skeletal muscle
... Polyubiquitination of lysine initiates proteasome-dependent degradation of target proteins, a process involved in both the pathogenic mechanisms of various diseases and in normal biological function. Recently we investigated polyubiquitination involves skeletal muscle atrophy by degradation of MyoD ...
... Polyubiquitination of lysine initiates proteasome-dependent degradation of target proteins, a process involved in both the pathogenic mechanisms of various diseases and in normal biological function. Recently we investigated polyubiquitination involves skeletal muscle atrophy by degradation of MyoD ...
Passenger Protein Determines Translocation Versus Retention in
... 100 mM sodium carbonate solution (pH, 11.4) on ice for 15 minutes. The samples were ultracentrifuged to separate soluble fraction from the membranous fraction. In experiment with methotrexate (MTX), 100 nM was used as the final concentration. All radioactive electrophoresed bands were visualized thr ...
... 100 mM sodium carbonate solution (pH, 11.4) on ice for 15 minutes. The samples were ultracentrifuged to separate soluble fraction from the membranous fraction. In experiment with methotrexate (MTX), 100 nM was used as the final concentration. All radioactive electrophoresed bands were visualized thr ...
Gene Section LCK (lymphocyte-specific protein tyrosine kinase) Atlas of Genetics and Cytogenetics
... membrane through its amino-terminus. This interaction is mediated by both myristic acid and palmitic acid that are bound to the amino terminal glycine and Cys-3 and/or Cys-5. The Unique region of Lck represents the domain possessing the greatest sequence diversity within this group of enzymes. This ...
... membrane through its amino-terminus. This interaction is mediated by both myristic acid and palmitic acid that are bound to the amino terminal glycine and Cys-3 and/or Cys-5. The Unique region of Lck represents the domain possessing the greatest sequence diversity within this group of enzymes. This ...
Table S1 Genes with similar expression patterns in Qing2
... Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport ...
... Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport ...
Differential scanning calorimetry as tool in observing thermal and
... The mechanisms of protein degradation by chemical or physical means have been extensively reviewed (Cueto et al., 2003; Gao et al., 2005). Protein structures are stabilized by non-covalent intramolecular interactions between amino acid side chains. The higher levels of protein structure are maintain ...
... The mechanisms of protein degradation by chemical or physical means have been extensively reviewed (Cueto et al., 2003; Gao et al., 2005). Protein structures are stabilized by non-covalent intramolecular interactions between amino acid side chains. The higher levels of protein structure are maintain ...
Soy Protein Based Green Composite: A Review
... causing the protein to precipitate out of aqueous solution [14]. It has been found that the soy protein could not form a film at or near its isoelectric point (pH 4.5) due to the coagulation. However, proteins can be denatured and unfolded at pH values away from their isoelectric point, thus exposin ...
... causing the protein to precipitate out of aqueous solution [14]. It has been found that the soy protein could not form a film at or near its isoelectric point (pH 4.5) due to the coagulation. However, proteins can be denatured and unfolded at pH values away from their isoelectric point, thus exposin ...
PowerPoint - Oregon State University
... • Identification of ToxB homologs will be carried out by PCR. • Screening of several ascomycete isolates • Primers have been designed for the Internal Transcribed Spacer (ITS) sequence and the Ptr ToxB sequence within the ORF ...
... • Identification of ToxB homologs will be carried out by PCR. • Screening of several ascomycete isolates • Primers have been designed for the Internal Transcribed Spacer (ITS) sequence and the Ptr ToxB sequence within the ORF ...
The UUAG-specific RNA Binding Protein, Heterogeneous Nuclear
... common groups of RNA binding proteins is the RBD class proteins (2). They possess a CS-RBD (consensus sequence-RNA binding domain) motif, which is typically 80 –90 amino acids. Two short sequences, RNP 2 octamer and RNP 1 hexamer, have been found to be conserved among different RBDs. Several RBDs ar ...
... common groups of RNA binding proteins is the RBD class proteins (2). They possess a CS-RBD (consensus sequence-RNA binding domain) motif, which is typically 80 –90 amino acids. Two short sequences, RNP 2 octamer and RNP 1 hexamer, have been found to be conserved among different RBDs. Several RBDs ar ...
BIOL 1322 General Nutrition
... Identify food claims (ie, what determines if a manufacturer can use the terms “reduced fat”, “low sodium”, etc. Understand adequacy, moderation, variety and balance Understand celiac disease, GERD, IBS, ulcerative colitis, Crohns, peptic ulcer, etc and be able to distinguish between and among Identi ...
... Identify food claims (ie, what determines if a manufacturer can use the terms “reduced fat”, “low sodium”, etc. Understand adequacy, moderation, variety and balance Understand celiac disease, GERD, IBS, ulcerative colitis, Crohns, peptic ulcer, etc and be able to distinguish between and among Identi ...
BIOL 1322 General Nutrition
... Identify food claims (ie, what determines if a manufacturer can use the terms “reduced fat”, “low sodium”, etc. Understand adequacy, moderation, variety and balance Understand celiac disease, GERD, IBS, ulcerative colitis, Crohns, peptic ulcer, etc and be able to distinguish between and among Identi ...
... Identify food claims (ie, what determines if a manufacturer can use the terms “reduced fat”, “low sodium”, etc. Understand adequacy, moderation, variety and balance Understand celiac disease, GERD, IBS, ulcerative colitis, Crohns, peptic ulcer, etc and be able to distinguish between and among Identi ...
Protein Function and Classification (Cont.) - EMBL-EBI
... example, where one entry might represent a protein family (such as the sugar transporter family), another entry might represent a specific subtype of that family (such as the glucose transporter type I subfamily). These relationships are stored in InterPro as hierarchies. When classifying proteins, ...
... example, where one entry might represent a protein family (such as the sugar transporter family), another entry might represent a specific subtype of that family (such as the glucose transporter type I subfamily). These relationships are stored in InterPro as hierarchies. When classifying proteins, ...
Cloning vectors for the expression of green fluorescent protein
... cal staining and light microscopy, and the subcellular resolution of this assay is sufficient for distinguishing nuclear from non-nuclear localization. On the other hand, indirect immunofluorescence detection provides a high spatial resolution, but relies on specific antibodies, and requires fixatio ...
... cal staining and light microscopy, and the subcellular resolution of this assay is sufficient for distinguishing nuclear from non-nuclear localization. On the other hand, indirect immunofluorescence detection provides a high spatial resolution, but relies on specific antibodies, and requires fixatio ...
Protein sequence databases
... • Symbol 'x' for a position where any amino acid is possible • Ambiguities are indicated by listing the acceptable amino acids in square parentheses '[ ]' • Ambiguities are indicated by listing the not acceptable amino acids in curly brackets '{ }' • Elements are separated by '-' • Repetition of an ...
... • Symbol 'x' for a position where any amino acid is possible • Ambiguities are indicated by listing the acceptable amino acids in square parentheses '[ ]' • Ambiguities are indicated by listing the not acceptable amino acids in curly brackets '{ }' • Elements are separated by '-' • Repetition of an ...
Differential display proteomic analysis of Picea meyeri pollen
... 100 nM LATB, pollen germination and tube growth was halfmaximally inhibited (Figure 2d). Under the confocal microscope, the actin filaments of untreated pollen tubes incubated in a culture medium for 28 h appeared in an axial array (Figure 3a). A ‘collar’ of actin, or a dense array of fine filaments ...
... 100 nM LATB, pollen germination and tube growth was halfmaximally inhibited (Figure 2d). Under the confocal microscope, the actin filaments of untreated pollen tubes incubated in a culture medium for 28 h appeared in an axial array (Figure 3a). A ‘collar’ of actin, or a dense array of fine filaments ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.