Hemoglobin
... • The best known is sickle-cell disease, which was the first human disease whose mechanism was understood at the molecular level. • A (mostly) separate set of diseases called thalassemias involves underproduction of normal and sometimes abnormal hemoglobins, through problems and mutations in globin ...
... • The best known is sickle-cell disease, which was the first human disease whose mechanism was understood at the molecular level. • A (mostly) separate set of diseases called thalassemias involves underproduction of normal and sometimes abnormal hemoglobins, through problems and mutations in globin ...
Towards the construction of Escherichia coli cell
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
Docking with GOLD Tutorial
... Configuration File User Guide at https://www.ccdc.cam.ac.uk/support-andresources/ccdcresources/gold_conf.pdf for more information. The GOLD configuration file specifies the location of: a. the prepared 3D coordinates of your ligand b. the prepared 3D coordinates of your protein An example of a prepa ...
... Configuration File User Guide at https://www.ccdc.cam.ac.uk/support-andresources/ccdcresources/gold_conf.pdf for more information. The GOLD configuration file specifies the location of: a. the prepared 3D coordinates of your ligand b. the prepared 3D coordinates of your protein An example of a prepa ...
Multiple Sequence Motifs in the Rubisco Small
... RbcS-nt:GFP) and E1a-tp fused to GFP (E1a-tp:GFP; Fig. 1A). RbcS-tp:GFP is a chimeric protein composed of the 79 N-terminal amino acids of the RbcS, which has been characterized in detail as a model cargo for the Toc159-dependent pathway (Lee et al., 2003; Smith et al., 2004), and GFP. E1a is the E1 ...
... RbcS-nt:GFP) and E1a-tp fused to GFP (E1a-tp:GFP; Fig. 1A). RbcS-tp:GFP is a chimeric protein composed of the 79 N-terminal amino acids of the RbcS, which has been characterized in detail as a model cargo for the Toc159-dependent pathway (Lee et al., 2003; Smith et al., 2004), and GFP. E1a is the E1 ...
Lecture 3 Isoelectric Focusing
... It will become -ve (pH>pI and migrate to the anode (+electrode attracts -ion)) As it passes through the gel, eg when it reaches pH 9 region, it becomes less -vely charged (pH not so much >pI) (+electrode does not attract protein so much – protein slows down) When reaches the pH 8 region, protein cha ...
... It will become -ve (pH>pI and migrate to the anode (+electrode attracts -ion)) As it passes through the gel, eg when it reaches pH 9 region, it becomes less -vely charged (pH not so much >pI) (+electrode does not attract protein so much – protein slows down) When reaches the pH 8 region, protein cha ...
calculating the structure-based phylogenetic relationship
... structures superiorly conserve homology and contain more biochemical information than their associated protein sequences, I hypothesize that utilizing the structure of a protein instead of its sequence will generate a superior dendrogram. Although no established or substantiated methodology exists ...
... structures superiorly conserve homology and contain more biochemical information than their associated protein sequences, I hypothesize that utilizing the structure of a protein instead of its sequence will generate a superior dendrogram. Although no established or substantiated methodology exists ...
Reivew, Hemoglobin
... Transport and Removal of CO2 Blood transports two forms of CO2 to the lungs: carbamino-hemoglobin and H2CO3/HCO3- (carbonic acidconjugate base pair) 1. Carbamino-hemoglobin: exposure to low pCO2 results in the reversal of the carbamination reaction by mass action and O2 binding is again favored. ...
... Transport and Removal of CO2 Blood transports two forms of CO2 to the lungs: carbamino-hemoglobin and H2CO3/HCO3- (carbonic acidconjugate base pair) 1. Carbamino-hemoglobin: exposure to low pCO2 results in the reversal of the carbamination reaction by mass action and O2 binding is again favored. ...
Leukaemia Section t(9;22)(q34;q11) in CML Atlas of Genetics and Cytogenetics
... 1- The crucial event lies on der(22), id est 5’ BCR/3’ ABL hybrid gene is pathogenic, while ABL/BCR may or may not be expressed; 2- Breakpoint in ABL is variable over a region of 200 kb, often between the two alternative exons 1b and 1a, sometimes 5’ of 1b or 3’ of 1a, but always 5’ of exon 2; 3- Br ...
... 1- The crucial event lies on der(22), id est 5’ BCR/3’ ABL hybrid gene is pathogenic, while ABL/BCR may or may not be expressed; 2- Breakpoint in ABL is variable over a region of 200 kb, often between the two alternative exons 1b and 1a, sometimes 5’ of 1b or 3’ of 1a, but always 5’ of exon 2; 3- Br ...
Supplementary Table 1
... Protein involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase ...
... Protein involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase ...
CONFERENCE REPORT FROM A NOBEL MINISYMPOSIUM/JIM
... Proteins and peptides of many sequences have been observed to change from their native structures into amyloid fibrils, and in line with those observations almost all proteins apparently contain segments with the potential to form amyloid-like fibrils. However, only a tiny fraction of all proteins ...
... Proteins and peptides of many sequences have been observed to change from their native structures into amyloid fibrils, and in line with those observations almost all proteins apparently contain segments with the potential to form amyloid-like fibrils. However, only a tiny fraction of all proteins ...
FEBS Letters 541, 176-177
... oscillations are sustained and can work as molecular clocks, as in the well-known cases of the circadian clock [1] and the cell cycle [2]. In other cases the oscillations in protein expression are connected with the response to external stimuli, as reported for protein p53 after induction by DNA dam ...
... oscillations are sustained and can work as molecular clocks, as in the well-known cases of the circadian clock [1] and the cell cycle [2]. In other cases the oscillations in protein expression are connected with the response to external stimuli, as reported for protein p53 after induction by DNA dam ...
New cell-based HTRF® assays for the exploration of Wnt signaling
... accumulation and the translocation of β-catenin into the nucleus. In the absence of Wnt signaling, cytoplasmic β-catenin is degraded by a destruction complex, which includes Axin, APC, PP2A, GSK3 and CK1 α. Within this complex, phosphorylation of β-catenin by GSK3 induces its ubiquitination followed ...
... accumulation and the translocation of β-catenin into the nucleus. In the absence of Wnt signaling, cytoplasmic β-catenin is degraded by a destruction complex, which includes Axin, APC, PP2A, GSK3 and CK1 α. Within this complex, phosphorylation of β-catenin by GSK3 induces its ubiquitination followed ...
Troubleshooting SDS-PAGE-0410
... There are no net negative charges on proteins, the protein will not move down the gel, ensure SDS has been added to the sample. The solution is acidic, add NaOH until the solution turns blue. There is too little bromophenol blue in the sample buffer. The buffers are too concentrated, dilute the buff ...
... There are no net negative charges on proteins, the protein will not move down the gel, ensure SDS has been added to the sample. The solution is acidic, add NaOH until the solution turns blue. There is too little bromophenol blue in the sample buffer. The buffers are too concentrated, dilute the buff ...
Molecular architecture of the glomerular slit
... protein the slit diaphragm. Several studies have provided support for the hypothesis that the outermost N-terminal Ig-like domains of nephrin from aadjacent podocytes interact homotypically [11,12], linking the foot processes dynamically together. This interaction most likely provides the structural ...
... protein the slit diaphragm. Several studies have provided support for the hypothesis that the outermost N-terminal Ig-like domains of nephrin from aadjacent podocytes interact homotypically [11,12], linking the foot processes dynamically together. This interaction most likely provides the structural ...
High Resolution Two-Dimensional Electrophoresis of Proteins*
... rather than across the entire detected and quantified by autoradiography. The reproducibility of the separation is sufficient to permit each spot on surface of the gel. Isoelectric focusing and a discontinuous SDS’ gel system (1) were chosen because of the high resolution of each one separation to b ...
... rather than across the entire detected and quantified by autoradiography. The reproducibility of the separation is sufficient to permit each spot on surface of the gel. Isoelectric focusing and a discontinuous SDS’ gel system (1) were chosen because of the high resolution of each one separation to b ...
61 - Lab Times
... In a recent PLoS ONE paper, Hakan Dortay and his colleagues from the Institute of Biochemistry and Biology at the University of Potsdam describe an interesting protein expression platform, based on E.coli, Pichia pastoris and the alternative hosts Klyveromycis lactis and Leishmania tarentolae (compl ...
... In a recent PLoS ONE paper, Hakan Dortay and his colleagues from the Institute of Biochemistry and Biology at the University of Potsdam describe an interesting protein expression platform, based on E.coli, Pichia pastoris and the alternative hosts Klyveromycis lactis and Leishmania tarentolae (compl ...
A fluorophore ligase for site-specific protein labeling inside living cells
... cross-react with E. coli LplA (15). To test for these possibilities, we performed coumarin labeling of LAP2-YFP (yellow fluorescent protein) in mammalian cell lysate and analyzed the samples by in-gel coumarin fluorescence imaging and anti-lipoic acid Western blotting. Fig. 2B shows that the endogen ...
... cross-react with E. coli LplA (15). To test for these possibilities, we performed coumarin labeling of LAP2-YFP (yellow fluorescent protein) in mammalian cell lysate and analyzed the samples by in-gel coumarin fluorescence imaging and anti-lipoic acid Western blotting. Fig. 2B shows that the endogen ...
Definition of Protein Superfamily
... Margaret O. Dayhoff introduced the term protein superfamily in 1974 [1,2,3]. Since that time, the sequences in the PIR-International Protein Sequence Database have been classified into protein superfamilies. Prior to about 1990, the superfamily classification permitted a sequence to be assigned to a ...
... Margaret O. Dayhoff introduced the term protein superfamily in 1974 [1,2,3]. Since that time, the sequences in the PIR-International Protein Sequence Database have been classified into protein superfamilies. Prior to about 1990, the superfamily classification permitted a sequence to be assigned to a ...
article MUNs - it is only a piece of the puzzle
... feeding, especially in AM / PM herd sample programs or herds milking 3X can lead to different “normal” values. ??Accuracy of the test - Because the MUN test is a NIR technology, it must be calibrated against a standard test to ensure that it is accurate. Recent work by Elliot Block and Daniel Lefebv ...
... feeding, especially in AM / PM herd sample programs or herds milking 3X can lead to different “normal” values. ??Accuracy of the test - Because the MUN test is a NIR technology, it must be calibrated against a standard test to ensure that it is accurate. Recent work by Elliot Block and Daniel Lefebv ...
Recombinant expression and characterisation of monofunctional S-
... domains. The deduced amino acid sequence of the bifunctional PfAdoMetDC/ODC predicted a molecular mass of 166 kDa for the polypeptide, whereas the recombinantly expressed enzyme had a molecular mass of -330 kDa. This suggested that the enzyme consists of a heterotetrameric structure derived from two ...
... domains. The deduced amino acid sequence of the bifunctional PfAdoMetDC/ODC predicted a molecular mass of 166 kDa for the polypeptide, whereas the recombinantly expressed enzyme had a molecular mass of -330 kDa. This suggested that the enzyme consists of a heterotetrameric structure derived from two ...
From Sequence to Structure
... signaling molecule. This function exploits the ability of proteins to present structurally and chemically diverse surfaces that can interact with other molecules with high specificity. Catalysis requires not only specific binding, to substrates and in some cases to regulatory molecules, but also spe ...
... signaling molecule. This function exploits the ability of proteins to present structurally and chemically diverse surfaces that can interact with other molecules with high specificity. Catalysis requires not only specific binding, to substrates and in some cases to regulatory molecules, but also spe ...
Cloning and sequencing of a gene encoding acidophilic amylase
... acidocaldarius ATCC 27009 chromosomal DNA was constructed as follows. Chromosomal DNA from B. acidocaldarius was isolated according to Marmur (1961). The DNA was digested partially with HaeIII. DNA fragments of 5.0 to 6.5 kb, collected from an agarose gel, were methylated with EcoIU methylase and li ...
... acidocaldarius ATCC 27009 chromosomal DNA was constructed as follows. Chromosomal DNA from B. acidocaldarius was isolated according to Marmur (1961). The DNA was digested partially with HaeIII. DNA fragments of 5.0 to 6.5 kb, collected from an agarose gel, were methylated with EcoIU methylase and li ...
The green fluorescent protein: discovery
... transcription factor when bound to its specific site or to non-specific sites on the chromosome. By varying the microscope monitoring time by the use of laser pulses of different lengths, the chromosomal residence time of the factor could be determined in the two cases, and thus the rate constants f ...
... transcription factor when bound to its specific site or to non-specific sites on the chromosome. By varying the microscope monitoring time by the use of laser pulses of different lengths, the chromosomal residence time of the factor could be determined in the two cases, and thus the rate constants f ...
Characterization of Phosphorylation Sites from the Activation Loop
... activation of MAPKs and kinases in general. Hyper-phosphorylation within the kinase activation loop by autophosphorylation following the initial activation by other upstream kinases may be a common mechanism for protein kinases to achieve full stimulation. ...
... activation of MAPKs and kinases in general. Hyper-phosphorylation within the kinase activation loop by autophosphorylation following the initial activation by other upstream kinases may be a common mechanism for protein kinases to achieve full stimulation. ...
Hedgehog signal transduction: recent findings Kent
... (Figure 2). More detailed examinations of Shh localization in megalin wild-type and mutant cells will be required to distinguish these posibilities. Hh-Np peptides are also acylated by palmitic acid on their N terminus and this acylation appears to have different effects in vertebrates versus invert ...
... (Figure 2). More detailed examinations of Shh localization in megalin wild-type and mutant cells will be required to distinguish these posibilities. Hh-Np peptides are also acylated by palmitic acid on their N terminus and this acylation appears to have different effects in vertebrates versus invert ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.