Proteomic analyses of Oryza sativa mature pollen reveal novel
... Chinese Academy of Sciences, Beijing, P. R. China ...
... Chinese Academy of Sciences, Beijing, P. R. China ...
Distributed Representations for Biological Sequence Analysis
... Apart from this encouraging performance on the protein classification task, a word embeddings based approach offers other advantages. First, the size of the data vector obtained by using embeddings is much smaller in comparison to the original sequence, thus making the vectors obtained amenable for ...
... Apart from this encouraging performance on the protein classification task, a word embeddings based approach offers other advantages. First, the size of the data vector obtained by using embeddings is much smaller in comparison to the original sequence, thus making the vectors obtained amenable for ...
the elastin gene
... partially solubilise the protein? How does increased crosslinking affect keratin's properties? 7. Keratin is the main component of hair. How are individual keratin molecules organised in a hair fibre? 8. Changes in keratin structure underlie temporary and permanent waving of hair. How? 9. If your ha ...
... partially solubilise the protein? How does increased crosslinking affect keratin's properties? 7. Keratin is the main component of hair. How are individual keratin molecules organised in a hair fibre? 8. Changes in keratin structure underlie temporary and permanent waving of hair. How? 9. If your ha ...
E-mail: - HAL
... not immediately recognizable, can be an alternative,4 albeit only partial, because the structural database, while large, is not complete. Today, large-scale genome sequencing projects are producing numerous protein sequences for which there are no homologues with a known structure. Under these circu ...
... not immediately recognizable, can be an alternative,4 albeit only partial, because the structural database, while large, is not complete. Today, large-scale genome sequencing projects are producing numerous protein sequences for which there are no homologues with a known structure. Under these circu ...
Gene Section GATA1 (GATA binding protein 1 (globin transcription factor1))
... of insertions, deletions and base substitution in exon 2 and vicinity and all result in elimination of the full length GATA1 protein with preservation of the GATA1s isoform. The presence of GATA1s in the absence of full length GATA1 blocks megakaryocytic differentiation and promote proliferation of ...
... of insertions, deletions and base substitution in exon 2 and vicinity and all result in elimination of the full length GATA1 protein with preservation of the GATA1s isoform. The presence of GATA1s in the absence of full length GATA1 blocks megakaryocytic differentiation and promote proliferation of ...
Co-translational Folding
... Escherichia coli tryptophan synthase b chains begin to fold during translation, even before appearance of the entire N-terminal domain showing conformation dependent antigenicity (Fedorov, A. N., Friguet, B., Djavadi-Ohaniance, L., Alakhov, Yu. B., and Goldberg, M. E. (1992) J. Mol. Biol. 228, 351–3 ...
... Escherichia coli tryptophan synthase b chains begin to fold during translation, even before appearance of the entire N-terminal domain showing conformation dependent antigenicity (Fedorov, A. N., Friguet, B., Djavadi-Ohaniance, L., Alakhov, Yu. B., and Goldberg, M. E. (1992) J. Mol. Biol. 228, 351–3 ...
Protein structure prediction
... - hydrogen bonds H—O (fast to form/break) • Non-bonded interactions between atoms: - electrostatic (long-range) - van der Waals (short range) • Confirmation that minimizes the free energy (Anfinsen’s hypothesis) François Fages ...
... - hydrogen bonds H—O (fast to form/break) • Non-bonded interactions between atoms: - electrostatic (long-range) - van der Waals (short range) • Confirmation that minimizes the free energy (Anfinsen’s hypothesis) François Fages ...
The Early Interaction of the Outer Membrane Protein PhoE with
... Bacterial Strains and Growth Conditions—The E. coli K12 strain MC4100 (F⫺ ⌬lacU169 araD136 rpsL thi relA) was used for the spheroplast labeling experiments and for the isolation of lysate and inner membrane vesicles (19). MC4100 and RC354c, containing a deletion in the skp gene (17), were used for t ...
... Bacterial Strains and Growth Conditions—The E. coli K12 strain MC4100 (F⫺ ⌬lacU169 araD136 rpsL thi relA) was used for the spheroplast labeling experiments and for the isolation of lysate and inner membrane vesicles (19). MC4100 and RC354c, containing a deletion in the skp gene (17), were used for t ...
Differential Accumulation Pattern of Met-rich beta
... Pattern of protein accumulation of β-zein in transgenic M. truncatula leaves was perfomed with Western analysis: EtOH-soluble fractions (equivalent to 25 ug of PBS-soluble fraction) from M. truncatula leaves were subjected to SDSPAGE followed by immunoblot analysis using b-zein antibodies. Lanes: M. ...
... Pattern of protein accumulation of β-zein in transgenic M. truncatula leaves was perfomed with Western analysis: EtOH-soluble fractions (equivalent to 25 ug of PBS-soluble fraction) from M. truncatula leaves were subjected to SDSPAGE followed by immunoblot analysis using b-zein antibodies. Lanes: M. ...
Cholecystokinin and Trypsin Responses of Larval Red Drum
... included in the culture system. The current work investigated the impact of soluble components of rotifers and algae on the CCK and trypsin responses of larval red drum at 6 and 10 days post hatch (DPH) as well as the response of red drum larvae to ingestion of inert polystyrene particles at 10 DPH. ...
... included in the culture system. The current work investigated the impact of soluble components of rotifers and algae on the CCK and trypsin responses of larval red drum at 6 and 10 days post hatch (DPH) as well as the response of red drum larvae to ingestion of inert polystyrene particles at 10 DPH. ...
Identification of a molecular component of the mitochondrial
... However, eukaryotic orthologues to Pat have not been identified in either the mitochondrial or nuclear genome [9]. An alternative scenario in eukaryotes could be that mitochondrial proteins are acetylated in the cytosol prior to mitochondrial import. However, as fasting and feeding result in a dynam ...
... However, eukaryotic orthologues to Pat have not been identified in either the mitochondrial or nuclear genome [9]. An alternative scenario in eukaryotes could be that mitochondrial proteins are acetylated in the cytosol prior to mitochondrial import. However, as fasting and feeding result in a dynam ...
Separation of Recombinant Human Erythropoietin (rEPO
... Recombinant human EPO protein is one of the most widely produced by many bio and pharmaceutical companies throughout the world for therapeutic agents. Erythropoietin protein (EPO) is a glycoprotein hormone found in plasma. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marr ...
... Recombinant human EPO protein is one of the most widely produced by many bio and pharmaceutical companies throughout the world for therapeutic agents. Erythropoietin protein (EPO) is a glycoprotein hormone found in plasma. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marr ...
The CamSol Method of Rational Design of Protein Mutants with
... represents also a major biotechnological issue, preventing many proteins to be produced at economically convenient yields [13,20,22]. Effective experimental approaches to improve protein solubility during recombinant expression include the use of weak promoters, modified growth media, low temperatur ...
... represents also a major biotechnological issue, preventing many proteins to be produced at economically convenient yields [13,20,22]. Effective experimental approaches to improve protein solubility during recombinant expression include the use of weak promoters, modified growth media, low temperatur ...
Microbial Genetics - University of Montana
... specifically to parC while the C-terminal interacts with ...
... specifically to parC while the C-terminal interacts with ...
Diapositiva 1 - ASCRS/ASOA 2008
... No finantial relationships between the authors and any company or person exist in regard to the present study ...
... No finantial relationships between the authors and any company or person exist in regard to the present study ...
HIV-1 Vpr-chloramphenicol acetyltransferase fusion proteins
... Several structural domains of Vpr have been shown to be involved in different activities of the protein. An Nterminal putative alpha-helical domain and a previously identified leucine/isoleucine rich region (LR-domain) in the middle region of Vpr have respectively been shown to be important for Vpr ...
... Several structural domains of Vpr have been shown to be involved in different activities of the protein. An Nterminal putative alpha-helical domain and a previously identified leucine/isoleucine rich region (LR-domain) in the middle region of Vpr have respectively been shown to be important for Vpr ...
No Slide Title
... • Translation of trp leader is needed for regulation – Mutation of AUG prevents transcription past the attenuator – Without translation, the 1:2 and 3:4 stem-loops form, and thus causing termination ...
... • Translation of trp leader is needed for regulation – Mutation of AUG prevents transcription past the attenuator – Without translation, the 1:2 and 3:4 stem-loops form, and thus causing termination ...
Comparison of the Structure of the Extrinsic 33 kDa Protein from
... (Koike and Inoue 1985, Enami et al. 2000). Thus, the structure of the 33 kDa protein has been considered to be largely conserved during evolution from cyanobacteria to higher plants. There are, however, some reports suggesting that the structure of the 33 kDa protein may be different, at least in it ...
... (Koike and Inoue 1985, Enami et al. 2000). Thus, the structure of the 33 kDa protein has been considered to be largely conserved during evolution from cyanobacteria to higher plants. There are, however, some reports suggesting that the structure of the 33 kDa protein may be different, at least in it ...
CHEMICAL SYNTHESIS OF PROTEINS : A TOOL FOR PROTEIN
... An ingenious stratagem useful to understand and modulate the structural and functional features of the proteins refers to the modification of their chemical structure. In this regard, the chemical synthesis of proteins appears a key tool, as it allows the unlimited modification of a polypeptide chai ...
... An ingenious stratagem useful to understand and modulate the structural and functional features of the proteins refers to the modification of their chemical structure. In this regard, the chemical synthesis of proteins appears a key tool, as it allows the unlimited modification of a polypeptide chai ...
THE THREE-DIMENSIONAL STRUCTURE OF PROTEINS
... can theoretically assume countless different conformations, and as a result the unfolded state of a protein is characterized by a high degree of conformational entropy. This entropy, and the hydrogen-bonding interactions of many groups in the polypeptide chain with solvent (water), tend to maintain ...
... can theoretically assume countless different conformations, and as a result the unfolded state of a protein is characterized by a high degree of conformational entropy. This entropy, and the hydrogen-bonding interactions of many groups in the polypeptide chain with solvent (water), tend to maintain ...
Product_Cheluminate_100915
... The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline condition followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. In the second step, ...
... The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline condition followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. In the second step, ...
Protein Structure and Interactions
... based on demonstrated in vitro activity. Whether these proteins behave as chaperones in vivo remains unclear. Here, we investigated the effect of HspB8 on the expression of Htt43Q, a fragment of the huntingtin protein containing a 43 glutamine repeat. Upon transient transfection, the polyglutamine p ...
... based on demonstrated in vitro activity. Whether these proteins behave as chaperones in vivo remains unclear. Here, we investigated the effect of HspB8 on the expression of Htt43Q, a fragment of the huntingtin protein containing a 43 glutamine repeat. Upon transient transfection, the polyglutamine p ...
PowerPoint 演示文稿
... To release mature fragments from polyprotein; To remove internal peptides as well as To trim both N- and C-termini. ...
... To release mature fragments from polyprotein; To remove internal peptides as well as To trim both N- and C-termini. ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.