NIH Public Access
... S6K1 is a member of the AGC subfamily of serine-threonine protein kinases, whereby catalytic activation requires dual phosphorylation of critical residues in the conserved T-loop (T229) and hydrophobic motif (HM; T389) peptide regions of its catalytic kinase domain (residues 1-398). In addition to i ...
... S6K1 is a member of the AGC subfamily of serine-threonine protein kinases, whereby catalytic activation requires dual phosphorylation of critical residues in the conserved T-loop (T229) and hydrophobic motif (HM; T389) peptide regions of its catalytic kinase domain (residues 1-398). In addition to i ...
Does Plant Cell Death Induced by Ptr ToxA Require Toxin Entry?
... Crystal Structure Ganapathy Sarma and Dr. Andy Karplus Biochemistry & Biophysics Dept. OSU ...
... Crystal Structure Ganapathy Sarma and Dr. Andy Karplus Biochemistry & Biophysics Dept. OSU ...
proteins: three-dimensional structure
... Proteins have historically been classified as either fibrous or globular, depending on their overall morphology. This dichotomy predates methods for determining protein structure on an atomic scale and does not do justice to proteins that contain both stiff, elongated, fibrous regions as well as mor ...
... Proteins have historically been classified as either fibrous or globular, depending on their overall morphology. This dichotomy predates methods for determining protein structure on an atomic scale and does not do justice to proteins that contain both stiff, elongated, fibrous regions as well as mor ...
Protein profiling of the dimorphic, pathogenic fungus,Penicillium
... companion spot in the opposite growth form. For example, spots B24 and B28 (Figs. 2, 3, 4) are considered upregulated "mould-phase" proteins. A second type of differentially expressed protein included those determined to be unique to a specific growth form. Such a protein was defined as one not havi ...
... companion spot in the opposite growth form. For example, spots B24 and B28 (Figs. 2, 3, 4) are considered upregulated "mould-phase" proteins. A second type of differentially expressed protein included those determined to be unique to a specific growth form. Such a protein was defined as one not havi ...
Amyotrophic lateral sclerosis: Protein
... of proper experimental designs, proteomic technologies can be very powerful at providing clues or answers to many crucial biological questions, including changes in protein expression, PTM, protein-protein interactions and protein activities. Traditionally, alterations in protein expression have bee ...
... of proper experimental designs, proteomic technologies can be very powerful at providing clues or answers to many crucial biological questions, including changes in protein expression, PTM, protein-protein interactions and protein activities. Traditionally, alterations in protein expression have bee ...
Data-driven docking for the study of biomolecular complexes
... With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particular on biomolecular interactions. Considering the huge number of such interactions, and their often weak and transient nature, conventional experimental methods such as Xray crysta ...
... With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particular on biomolecular interactions. Considering the huge number of such interactions, and their often weak and transient nature, conventional experimental methods such as Xray crysta ...
Computer-Based Design of Novel Protein Structures
... using the query sequence and its predicted secondary structure. For protein design there is no query sequence, but the fragments can be picked using the desired secondary structure at each residue position. In some cases it may not be clear where one element of secondary structure should begin and a ...
... using the query sequence and its predicted secondary structure. For protein design there is no query sequence, but the fragments can be picked using the desired secondary structure at each residue position. In some cases it may not be clear where one element of secondary structure should begin and a ...
C-terminal Truncation of p21H Preserves Crucial Kinetic and
... (18).Transformation was done according to themethod of Hanahan (19) with frozen cells or according to theCaC12method (18). Site-directed mutagenesis was performed according to themethod of Taylor and Eckstein (20),using Ex0111 from New England Biolabs and DNA polymerase (Klenowfragment) from Du Pont ...
... (18).Transformation was done according to themethod of Hanahan (19) with frozen cells or according to theCaC12method (18). Site-directed mutagenesis was performed according to themethod of Taylor and Eckstein (20),using Ex0111 from New England Biolabs and DNA polymerase (Klenowfragment) from Du Pont ...
The Transition from Stiff to Compliant Materials in Squid Beaks
... and B). Only the untanned region differed in composition, with the Asx content being considerably higher and that of the other amino acids being somewhat lower than the corresponding values in the tanned regions. This disparity may ...
... and B). Only the untanned region differed in composition, with the Asx content being considerably higher and that of the other amino acids being somewhat lower than the corresponding values in the tanned regions. This disparity may ...
Low Protein Diet - Hamilton Health Sciences
... become easier as he or she grows older and you become more comfortable with the diet. Teach your child about the low protein diet as soon as he or she is old enough to understand it. A low protein diet is recommended for life, however the amount of protein allowed may change as your child grows up. ...
... become easier as he or she grows older and you become more comfortable with the diet. Teach your child about the low protein diet as soon as he or she is old enough to understand it. A low protein diet is recommended for life, however the amount of protein allowed may change as your child grows up. ...
Journal of Agricultural and Food Chemistry
... Gorinstein et al., 1996). There also are investigations showing comparisons among soybean, oats, and amaranth in relation to nutritional, structural, and functional properties (Gorinstein, 1993; Marcone and Yada, 1992; Segura-Nieto et al., 1994). To date, there is no information on the relative stab ...
... Gorinstein et al., 1996). There also are investigations showing comparisons among soybean, oats, and amaranth in relation to nutritional, structural, and functional properties (Gorinstein, 1993; Marcone and Yada, 1992; Segura-Nieto et al., 1994). To date, there is no information on the relative stab ...
Biochemical and Molecular Characterization of the Chicken
... Biosystems Ltd., Kent, UK). The column was washed with '~100 ml of buffer B-10; the 23-kD protein was collected in the non-adsorbed column fractions. Ammonium sulfate was added to the non-adsorbed fractions enriched in the 23-kD protein to a final concentration of 20% saturation and mixed briefly, T ...
... Biosystems Ltd., Kent, UK). The column was washed with '~100 ml of buffer B-10; the 23-kD protein was collected in the non-adsorbed column fractions. Ammonium sulfate was added to the non-adsorbed fractions enriched in the 23-kD protein to a final concentration of 20% saturation and mixed briefly, T ...
A Method To Define the Carboxyl Terminal of Proteins
... critical role in a variety of biological processes. For example, prenylation, which occurs on cysteine residues near the Cterminus, functions to anchor proteins to lipid membranes,1 while aberrant C-terminal processing of the amyloid β-protein in humans is believed to be involved in the pathogenesis ...
... critical role in a variety of biological processes. For example, prenylation, which occurs on cysteine residues near the Cterminus, functions to anchor proteins to lipid membranes,1 while aberrant C-terminal processing of the amyloid β-protein in humans is believed to be involved in the pathogenesis ...
Print - Circulation Research
... we chose to use isolated hearts in vitro. Because the effects on lysosomal function might be slow to develop, we chose to use an in vitro model which can function stably for many hours to days, i.e., the fetal mouse heart in organ culture.2 A second problem in approaching this question is to choose ...
... we chose to use isolated hearts in vitro. Because the effects on lysosomal function might be slow to develop, we chose to use an in vitro model which can function stably for many hours to days, i.e., the fetal mouse heart in organ culture.2 A second problem in approaching this question is to choose ...
manualE6901
... In addition, with the use of pTXB1, native recombinant proteins that possess a reactive C-terminal thioester can be isolated for applications, including protein semisynthesis and site-specific labeling [3,7, Intein Mediated Protein Ligation ...
... In addition, with the use of pTXB1, native recombinant proteins that possess a reactive C-terminal thioester can be isolated for applications, including protein semisynthesis and site-specific labeling [3,7, Intein Mediated Protein Ligation ...
The significance of biochemical and molecular sample integrity in
... regions. However, these analyses mainly detected high levels of peptide fragments from abundant proteins, not likely reflecting the in vivo composition of the neuropeptides. The relatively short postmortem interval from sacrifice to tissue preparation (3 min) was sufficient to produce a large amount ...
... regions. However, these analyses mainly detected high levels of peptide fragments from abundant proteins, not likely reflecting the in vivo composition of the neuropeptides. The relatively short postmortem interval from sacrifice to tissue preparation (3 min) was sufficient to produce a large amount ...
In vitro gastrointestinal digestion study of a novel bio-tofu
... catalyzes the transfer reaction between many proteins by crosslinking of the amino acid residues of protein bound glutamine and lysine (Hsieh et al., 2014). Soy proteins and caseins are known to be good substrates for MTGase. Enzymatic modification of proteins by MTGase provides protein to distribut ...
... catalyzes the transfer reaction between many proteins by crosslinking of the amino acid residues of protein bound glutamine and lysine (Hsieh et al., 2014). Soy proteins and caseins are known to be good substrates for MTGase. Enzymatic modification of proteins by MTGase provides protein to distribut ...
Electrophoretic Properties of Native Proteins
... Proteins exhibit many different three-dimensional shapes and complex folding patterns which are determined by their amino acid sequence and post translational processing such as adding carbohydrate residues or prosthetic groups. The precise three-dimensional configuration of a protein is critical to ...
... Proteins exhibit many different three-dimensional shapes and complex folding patterns which are determined by their amino acid sequence and post translational processing such as adding carbohydrate residues or prosthetic groups. The precise three-dimensional configuration of a protein is critical to ...
Basic Structure of Proteins
... H2N-Glu-Ala-Val-Ser-Leu-Ala-Lys-Cys-COOH H2N-Ala-Glu-Val-Ser-Ala-Leu-Lys-Cys-COOH ...
... H2N-Glu-Ala-Val-Ser-Leu-Ala-Lys-Cys-COOH H2N-Ala-Glu-Val-Ser-Ala-Leu-Lys-Cys-COOH ...
Tunicates and not cephalochordates are the closest living relatives
... grouped with echinoderms, a hypothesis that needs to be tested with additional data. This new phylogenetic scheme prompts a reappraisal of both morphological and palaeontological data and has important implications for the interpretation of developmental and genomic studies in which tunicates and ce ...
... grouped with echinoderms, a hypothesis that needs to be tested with additional data. This new phylogenetic scheme prompts a reappraisal of both morphological and palaeontological data and has important implications for the interpretation of developmental and genomic studies in which tunicates and ce ...
BRNO UNIVERSITY OF TECHNOLOGY FACULTY OF
... world. However, only 2 % of barley is used for human food at present, mainly in the developing world. It is used as an animal feed more likely, and the worldwide greatest use of barley is for malting purposes, most specifically for the brewing industry.4,5 Malting is controlled germination of cereal ...
... world. However, only 2 % of barley is used for human food at present, mainly in the developing world. It is used as an animal feed more likely, and the worldwide greatest use of barley is for malting purposes, most specifically for the brewing industry.4,5 Malting is controlled germination of cereal ...
as PDF
... Extracellular Regulation of TGF-β Signaling The TGF-β family of signaling proteins is widely represented throughout the animal kingdom and consists of more than 40 known members including TGF-β isoforms, bone morphogenetic proteins (BMPs), growth differentiation factors (GDFs), activins and inhibins ...
... Extracellular Regulation of TGF-β Signaling The TGF-β family of signaling proteins is widely represented throughout the animal kingdom and consists of more than 40 known members including TGF-β isoforms, bone morphogenetic proteins (BMPs), growth differentiation factors (GDFs), activins and inhibins ...
Cell and Molecular Biology
... Some actin-binding proteins such as a-actinin, villin and fimbrin bind actin filaments into parallel bundles. Depending on the length of a crosslinking protein, or the distance between actin-binding domains, actin filaments in parallel bundles may be held close together, or may be far enough apart ...
... Some actin-binding proteins such as a-actinin, villin and fimbrin bind actin filaments into parallel bundles. Depending on the length of a crosslinking protein, or the distance between actin-binding domains, actin filaments in parallel bundles may be held close together, or may be far enough apart ...
Structure and Function of the Groucho Gene Family and Encoded
... The Groucho protein, once recruited as a non-DNA binding corepressor, can repress both basal and activated transcription for specific subsets of DNA-binding transcription factors. Using a yeast two-hybrid assay, in vitro association and pull-down experiments, human TLE1 protein was demonstrated to i ...
... The Groucho protein, once recruited as a non-DNA binding corepressor, can repress both basal and activated transcription for specific subsets of DNA-binding transcription factors. Using a yeast two-hybrid assay, in vitro association and pull-down experiments, human TLE1 protein was demonstrated to i ...
Chapter 5A Lecture
... protein. Ligands can be any type of molecule, including another protein. Proteins that bind ligands do so at sequences called the binding site. The binding site is complementary in shape to the ligand that is bound. The degree of complementarity determines the binding specificity and strength. Most ...
... protein. Ligands can be any type of molecule, including another protein. Proteins that bind ligands do so at sequences called the binding site. The binding site is complementary in shape to the ligand that is bound. The degree of complementarity determines the binding specificity and strength. Most ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.