EVOLUTIONARILY RELATED INSERTION PATHWAYS OF
... presequences. The first part of these presequences target the protein to the lumen of the organelle, whereas the second part mediates membrane integration. How might topogenic sequences operate? Over two decades ago Blobel (1980), in his signal hypothesis, proposed that membrane proteins contain sig ...
... presequences. The first part of these presequences target the protein to the lumen of the organelle, whereas the second part mediates membrane integration. How might topogenic sequences operate? Over two decades ago Blobel (1980), in his signal hypothesis, proposed that membrane proteins contain sig ...
Abiotic stress in plants: Late Embryogenesis Abundant proteins Imen Amara
... plant ABA levels which induce the expression of stress-related genes and adaptive physiological responses (Cramer et al. 2011; Raghavendra et al. 2010). In seeds, ABA is a major factor regulating seed dormancy. ABA-deficient seeds as the viviparous mutants in maize show reduced dormancy whereas ove ...
... plant ABA levels which induce the expression of stress-related genes and adaptive physiological responses (Cramer et al. 2011; Raghavendra et al. 2010). In seeds, ABA is a major factor regulating seed dormancy. ABA-deficient seeds as the viviparous mutants in maize show reduced dormancy whereas ove ...
Membrane Filtration in the Dairy Industry
... nanofiltration technology rejects lactose but allows passage of various minerals, the milk will maintain most of its original composition and ...
... nanofiltration technology rejects lactose but allows passage of various minerals, the milk will maintain most of its original composition and ...
Prokaryotic proteins of antioxidant defense in Trichomonas vaginalis
... anion and free radical, superoxide O2-. Superoxide causes the release of iron from iron-containing clusters of enzymes (FeS clusters) (208) or reduces Fe3+ via first step of Haber-Weiss reaction. In both cases reduced Fe2+ can participate in the Fenton reaction (Fe2+ + H2O2→Fe3+ + OH + OH-), genera ...
... anion and free radical, superoxide O2-. Superoxide causes the release of iron from iron-containing clusters of enzymes (FeS clusters) (208) or reduces Fe3+ via first step of Haber-Weiss reaction. In both cases reduced Fe2+ can participate in the Fenton reaction (Fe2+ + H2O2→Fe3+ + OH + OH-), genera ...
Utilisation of Whey
... Some of them represent the normal antibody components of the cow and are the same as those found in the bloodstream; however, there is a secretory form of IgA which is produced in the mammary gland in response to infectious organisms. From a physiological standpoint, the immunoglobulins are importan ...
... Some of them represent the normal antibody components of the cow and are the same as those found in the bloodstream; however, there is a secretory form of IgA which is produced in the mammary gland in response to infectious organisms. From a physiological standpoint, the immunoglobulins are importan ...
Biochemical, biophysical and interaction studies of the stress
... Firstly I would like to thank all my supervisors, Dr Alexander Golovanov, Dr Costas Demonacos and Dr Stephen Prince for their continual guidance throughout this PhD. I would also like to thank my adviser Professor Andrew Doig for all his support and advice on PhD related issues and CD experiments. A ...
... Firstly I would like to thank all my supervisors, Dr Alexander Golovanov, Dr Costas Demonacos and Dr Stephen Prince for their continual guidance throughout this PhD. I would also like to thank my adviser Professor Andrew Doig for all his support and advice on PhD related issues and CD experiments. A ...
Mechanisms and applications of disulfide bond formation
... these proteins contain disulfide bonds in their native state, covalent links formed between the thiol groups of cysteine residues. In many proteins, disulfide bonds play an essential role in folding, stabilizing structure and the function of the protein. Therefore, understanding the pathways of disu ...
... these proteins contain disulfide bonds in their native state, covalent links formed between the thiol groups of cysteine residues. In many proteins, disulfide bonds play an essential role in folding, stabilizing structure and the function of the protein. Therefore, understanding the pathways of disu ...
SBT202 - SibTech, Inc.
... The EGF/Si reagent carries single Cy5.5 dye (FW 1128Da; GE Healthcare) attached site-specifically to C4 residue of Cys-tag. One vial contains 150 g of RP-HPLC purified lyophilized EGF/Si Purity: This EGF/Si preparation contains >98% pure protein that migrates as a single band with an apparent molec ...
... The EGF/Si reagent carries single Cy5.5 dye (FW 1128Da; GE Healthcare) attached site-specifically to C4 residue of Cys-tag. One vial contains 150 g of RP-HPLC purified lyophilized EGF/Si Purity: This EGF/Si preparation contains >98% pure protein that migrates as a single band with an apparent molec ...
Lactococcus lactis as expression host for the biosynthetic
... strains to stimulate the incorporation of Trp analogues [18]. This approach was explored for L. lactis PA1001 in CDM. A concentration of 0.2 mg/ml of IAA completely inhibited the growth of L. lactis PA1001 (Figure 3A). However, addition of Trp did not elevate the IAA-inhibitory effect, suggesting th ...
... strains to stimulate the incorporation of Trp analogues [18]. This approach was explored for L. lactis PA1001 in CDM. A concentration of 0.2 mg/ml of IAA completely inhibited the growth of L. lactis PA1001 (Figure 3A). However, addition of Trp did not elevate the IAA-inhibitory effect, suggesting th ...
Phylogenetic and Conservational Analyses of Dengue Non
... Dengue virus (DENV) is a RNA virus of the family Flaviviridae and exists as four closely related antigenically distinct serotypes denoted DENV-1 to DENV4. Genetic variation within and among each of these four serotypes has been observed to cause implications for pathogenesis of DENV infection, varia ...
... Dengue virus (DENV) is a RNA virus of the family Flaviviridae and exists as four closely related antigenically distinct serotypes denoted DENV-1 to DENV4. Genetic variation within and among each of these four serotypes has been observed to cause implications for pathogenesis of DENV infection, varia ...
Distinct C-terminal Amino Acid Sequence Motifs Serve
... Tail-anchored (TA) proteins are a unique class of functionally diverse membrane proteins that are defined by their single C-terminal transmembrane domain and their ability to insert post-translationally into specific organelles in an Ncytosol-CIMS orientation. While in recent years considerable prog ...
... Tail-anchored (TA) proteins are a unique class of functionally diverse membrane proteins that are defined by their single C-terminal transmembrane domain and their ability to insert post-translationally into specific organelles in an Ncytosol-CIMS orientation. While in recent years considerable prog ...
Insights into interactions between poly(ethylene glycol) and proteins
... polymer of sucrose monomers that are cross-linked by epichlorohydrins; it is thought to take on a spherical shape13-16. All four crowders have been extensively studied, both experimentally and theoretically. In addition to their use in crowding studies (see below), some of these polymers have also b ...
... polymer of sucrose monomers that are cross-linked by epichlorohydrins; it is thought to take on a spherical shape13-16. All four crowders have been extensively studied, both experimentally and theoretically. In addition to their use in crowding studies (see below), some of these polymers have also b ...
Figure S1. Selection of T-DNA lines. (a) Position of T
... wild-type (wt), rpx-1 mutant, 35S::RPX (ox) and 35S::RPX∆C (oxc) plants. Plants were grown in soil at 21°C under long-day condition for 5 weeks. Figure S2. Analysis of the hydrophobic C-terminus of the RPX protein and phenotype of the transgenic 35S::RPX∆C plants. (a) Hydropathy plot of the amino a ...
... wild-type (wt), rpx-1 mutant, 35S::RPX (ox) and 35S::RPX∆C (oxc) plants. Plants were grown in soil at 21°C under long-day condition for 5 weeks. Figure S2. Analysis of the hydrophobic C-terminus of the RPX protein and phenotype of the transgenic 35S::RPX∆C plants. (a) Hydropathy plot of the amino a ...
Cell Division: The Place and Time of Cytokinesis Dispatch
... factors (GEFs) and the GTPase-activating proteins (GAPs): a GEF activates Rho1 by catalyzing the uptake of GTP, while a GAP stimulates the GTPase activity of Rho1 leading to its inactivation. In Drosophila the Rho1-GEF is Pebble (Pbl) a protein known to be essential for cytokinesis [2,3]. Two recent ...
... factors (GEFs) and the GTPase-activating proteins (GAPs): a GEF activates Rho1 by catalyzing the uptake of GTP, while a GAP stimulates the GTPase activity of Rho1 leading to its inactivation. In Drosophila the Rho1-GEF is Pebble (Pbl) a protein known to be essential for cytokinesis [2,3]. Two recent ...
Proteome analysis of tobacco BY-2 cell culture - ETH E
... distinguish plant cells from those of other eukaryotes. The origin and evolution of plastids has been an important subject in biological sciences. Plastids represent the endosymbiotic remnants of a free-living cyanobacterial progenitor, which lost the vast majority of its ancestral cyanobacterial ge ...
... distinguish plant cells from those of other eukaryotes. The origin and evolution of plastids has been an important subject in biological sciences. Plastids represent the endosymbiotic remnants of a free-living cyanobacterial progenitor, which lost the vast majority of its ancestral cyanobacterial ge ...
108. Regulation of the L-arabinose Operon in Escherichia coli, in
... strengthened or weakened its binding to either of these two sites. Furthermore, the mutations could be imagined to lie in either the binding sites on the domains, or in the arm itself. Hence there are eight classes of mutations that might be found that would directly affect arm positioning and which ...
... strengthened or weakened its binding to either of these two sites. Furthermore, the mutations could be imagined to lie in either the binding sites on the domains, or in the arm itself. Hence there are eight classes of mutations that might be found that would directly affect arm positioning and which ...
Regulation of Cytochrome bd Expression in Mycobacterium
... Mycobacteria are Gram-positive and acid-fast bacteria, belonging to the phylum of Actinobacteria. Mycobacteria possess G + C rich genomes with a content of about 62-70% (24). They are considered to be obligate aerobes and have a rod-shaped appearance of about 0.3 - 0.5 µm in diameter and of variable ...
... Mycobacteria are Gram-positive and acid-fast bacteria, belonging to the phylum of Actinobacteria. Mycobacteria possess G + C rich genomes with a content of about 62-70% (24). They are considered to be obligate aerobes and have a rod-shaped appearance of about 0.3 - 0.5 µm in diameter and of variable ...
Structure and function of the eukaryotic ADP
... extensively tested by 31P-NMR, where the enzyme proved to be highly specific for Dglucose. Residues important for catalysis have been modified by site-directed mutagenesis and the variants of H. sapiens ADPGK were purified and kinetic parameters determined. A single crystal was obtained from a trunc ...
... extensively tested by 31P-NMR, where the enzyme proved to be highly specific for Dglucose. Residues important for catalysis have been modified by site-directed mutagenesis and the variants of H. sapiens ADPGK were purified and kinetic parameters determined. A single crystal was obtained from a trunc ...
EP 1790660 B1
... [0020] The method will typically involve the steps of: obtaining nucleic acid encoding a protein of the invention; manipulating said nucleic acid to remove at least one domain from within the protein. The resulting nucleic acid may be inserted into an expression vector, or may already be part of an ...
... [0020] The method will typically involve the steps of: obtaining nucleic acid encoding a protein of the invention; manipulating said nucleic acid to remove at least one domain from within the protein. The resulting nucleic acid may be inserted into an expression vector, or may already be part of an ...
Protein 3D-structure analysis
... http://www.rcsb.org/pdb/explore/explore.do?structureId=1THC Similar structure: 2H6U What is the function of transthyretin, respectively of other proteins with similar structure? Have a look at the corresponding UniProt entries! How are they classified at CATH Use text search http://www.cathdb.info/c ...
... http://www.rcsb.org/pdb/explore/explore.do?structureId=1THC Similar structure: 2H6U What is the function of transthyretin, respectively of other proteins with similar structure? Have a look at the corresponding UniProt entries! How are they classified at CATH Use text search http://www.cathdb.info/c ...
Keyhole Limpet Hemocyanin ( KLH )
... Add gently 2 mL deionized water per 20mg vial under agitation yields an opalescent solution of KLH at 10 mg/mL. Do not vortex or agitate vigorously. For further dilutions , use appropriate buffer (i.e. PBS) and maintain the magnesium concentration at a level higher than 10 mM. Reconstituted protein ...
... Add gently 2 mL deionized water per 20mg vial under agitation yields an opalescent solution of KLH at 10 mg/mL. Do not vortex or agitate vigorously. For further dilutions , use appropriate buffer (i.e. PBS) and maintain the magnesium concentration at a level higher than 10 mM. Reconstituted protein ...
Cambridge Isotope Laboratories, Inc. RefeRences 1. Shadforth, I.P.
... the mass spectrometer they are easily distinguished by their mass. Algorithms are then used to extract the light and heavy peptide ion chromatograms, which represent the peptide’s abundance. The light / heavy ratios are used to infer relative protein abundance. By mixing the same labeled protein sta ...
... the mass spectrometer they are easily distinguished by their mass. Algorithms are then used to extract the light and heavy peptide ion chromatograms, which represent the peptide’s abundance. The light / heavy ratios are used to infer relative protein abundance. By mixing the same labeled protein sta ...
introduction figures
... In pPE5-399, the catalytic domain of the tox A gene is under control of a T7 promoter. The PE24 gene in this plasmid contains an Omp A signal sequence that is cleaved upon secretion of PE24 into the periplasm. The plasmid also contains repeat of the trinucleotide, CAT, that codes for a poly His seq ...
... In pPE5-399, the catalytic domain of the tox A gene is under control of a T7 promoter. The PE24 gene in this plasmid contains an Omp A signal sequence that is cleaved upon secretion of PE24 into the periplasm. The plasmid also contains repeat of the trinucleotide, CAT, that codes for a poly His seq ...
Kinetic Role of Helix Caps in Protein Folding Is Context
... not native in the p53 TS between the unfolded and the dimeric intermediate states, but almost all residues are highly nativelike in TS from the dimeric intermediate to the tetrameric native state (28). A range of kinetic effects has also been seen in single amino acid mutations at both helix ends in ...
... not native in the p53 TS between the unfolded and the dimeric intermediate states, but almost all residues are highly nativelike in TS from the dimeric intermediate to the tetrameric native state (28). A range of kinetic effects has also been seen in single amino acid mutations at both helix ends in ...
Lipid Rafts Presentation
... Pike, Linda J. "The Challenge of Lipid Rafts." Journal of Lipid Research Oct (2008): 1-17. Simons, Kai, and Ehehalt, R. "Cholesterol, lipid rafts, and disease." The Journal of Clinical Invesigation 110 ...
... Pike, Linda J. "The Challenge of Lipid Rafts." Journal of Lipid Research Oct (2008): 1-17. Simons, Kai, and Ehehalt, R. "Cholesterol, lipid rafts, and disease." The Journal of Clinical Invesigation 110 ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.