NIH Public Access - The Scripps Research Institute
... (PCPs), which are ~75 amino acid long domains (predicted bioinformatically) or ~80–95 amino acid long proteins that are posttranslationally modified with a 4′-phosphopantetheinyl (ppant) group from coenzyme A by phosphopantetheinyl transferases (PPTase), also known as holo-ACP or holo-PCP synthases. ...
... (PCPs), which are ~75 amino acid long domains (predicted bioinformatically) or ~80–95 amino acid long proteins that are posttranslationally modified with a 4′-phosphopantetheinyl (ppant) group from coenzyme A by phosphopantetheinyl transferases (PPTase), also known as holo-ACP or holo-PCP synthases. ...
ZRII ACHIEVE™ PRODUCT INFORMATION
... is currently the most advanced protein quality measurement scale. On this scale, the highest score possible for any food is 1.0. Soy-protein isolate came back with a perfect 1.0 score, meaning soy protein most bio-available (nutritionally useful) for the human body. What are the benefits of amino ac ...
... is currently the most advanced protein quality measurement scale. On this scale, the highest score possible for any food is 1.0. Soy-protein isolate came back with a perfect 1.0 score, meaning soy protein most bio-available (nutritionally useful) for the human body. What are the benefits of amino ac ...
Clustering of Proteins
... currently used in practice employ evolutionary relationships between sequences to predict functional properties. Clustering of proteins is one such method for determining evolutionary relationships between proteins and thereby inferring functional properties. To perform a clustering on the proteins, ...
... currently used in practice employ evolutionary relationships between sequences to predict functional properties. Clustering of proteins is one such method for determining evolutionary relationships between proteins and thereby inferring functional properties. To perform a clustering on the proteins, ...
Identification of Surface Residues Involved in Protein
... rectly identified. With this level of success, predictions generated using this approach should be valuable for guiding experimental investigations into the roles of specific residues of a protein in its interaction with other proteins. Detailed examination of the predicted interface residues in th ...
... rectly identified. With this level of success, predictions generated using this approach should be valuable for guiding experimental investigations into the roles of specific residues of a protein in its interaction with other proteins. Detailed examination of the predicted interface residues in th ...
BIOINFORMATICS ORIGINAL PAPER Going from where to why—interpretable prediction of protein subcellular localization
... cells, and hence a major research topic in biology. After being synthesized, proteins are transported into different compartments depending on their molecular role within the cell. Some proteins are even transported to multiple sites. Protein localization is often mediated by sorting signals or sort ...
... cells, and hence a major research topic in biology. After being synthesized, proteins are transported into different compartments depending on their molecular role within the cell. Some proteins are even transported to multiple sites. Protein localization is often mediated by sorting signals or sort ...
maize silage sampling and interpretation of analysis
... board, divide the sample at right angles into four piles to achieve a final sample size of 0.5kg. Place the entire 0.5 kg sample into a clean plastic bag and ensure as much of the air is removed, as possible. For further information see the Forage Analytical Assurance Group website - http://www.faag ...
... board, divide the sample at right angles into four piles to achieve a final sample size of 0.5kg. Place the entire 0.5 kg sample into a clean plastic bag and ensure as much of the air is removed, as possible. For further information see the Forage Analytical Assurance Group website - http://www.faag ...
CIP Posters - International Potato Center
... The groups previously classified in Arabidopsis [1] were identified: Group 1 proteins contain 2 WRKY domains and based on the C-terminal WRKY domain alone do not form a clearly supported group in phylogenetic tree. Part of the group 2b proteins cluster together with group 2a proteins. However, these ...
... The groups previously classified in Arabidopsis [1] were identified: Group 1 proteins contain 2 WRKY domains and based on the C-terminal WRKY domain alone do not form a clearly supported group in phylogenetic tree. Part of the group 2b proteins cluster together with group 2a proteins. However, these ...
Silk-inspired polymers and proteins - Pure
... Multiblock copolymers (with MWs between 27 and 40 kDa) composed of a rigid aromatic spacer (derivatives of phenoxathiin or xanthene) that templated parallel or antiparallel β-sheet formation of 2 pendant GlyAlaGlyAla tetrapeptides, linked to flexible blocks (of short alkane or ethylene glycol chains ...
... Multiblock copolymers (with MWs between 27 and 40 kDa) composed of a rigid aromatic spacer (derivatives of phenoxathiin or xanthene) that templated parallel or antiparallel β-sheet formation of 2 pendant GlyAlaGlyAla tetrapeptides, linked to flexible blocks (of short alkane or ethylene glycol chains ...
Class 11 Time : 1 hr. 14-5-2015 First Unit Test in ENGLISH M. Marks
... Spirulina is a filamentous organism belonging to the group of blue-green algae known as cyanobacteria. It is very rich in proteins. Spirulina farms can produce about 20 times more protein per unit area than soybeans. In addition, Spirulina lacks cellulose in its walls and this makes its protein easi ...
... Spirulina is a filamentous organism belonging to the group of blue-green algae known as cyanobacteria. It is very rich in proteins. Spirulina farms can produce about 20 times more protein per unit area than soybeans. In addition, Spirulina lacks cellulose in its walls and this makes its protein easi ...
Proteomic sensitivity to dietary manipulations in rainbow trout
... excretion was increased as well as the activities of hepatic glutamate dehydrogenase and aspartate amino transferase (ASAT). No differences were found in free amino acid pools in either liver or muscle between diets. Protein extraction followed by high-resolution two-dimensional electrophoresis, cou ...
... excretion was increased as well as the activities of hepatic glutamate dehydrogenase and aspartate amino transferase (ASAT). No differences were found in free amino acid pools in either liver or muscle between diets. Protein extraction followed by high-resolution two-dimensional electrophoresis, cou ...
Going from where to why—interpretable
... cells, and hence a major research topic in biology. After being synthesized, proteins are transported into different compartments depending on their molecular role within the cell. Some proteins are even transported to multiple sites. Protein localization is often mediated by sorting signals or sort ...
... cells, and hence a major research topic in biology. After being synthesized, proteins are transported into different compartments depending on their molecular role within the cell. Some proteins are even transported to multiple sites. Protein localization is often mediated by sorting signals or sort ...
Increased MS Protein Identifi cation Rates Using 75 cm Long nano
... setups. At the beginning of the chromatogram a small shift in the retention time (RT) is observable due to the increased volume of the 75 cm column. The peak profiles among replicates were almost identical and peptide retention time shifts of less than 1 minute were observed even when employing a 24 ...
... setups. At the beginning of the chromatogram a small shift in the retention time (RT) is observable due to the increased volume of the 75 cm column. The peak profiles among replicates were almost identical and peptide retention time shifts of less than 1 minute were observed even when employing a 24 ...
Improving protein fold recognition with hybrid
... and proteins of known structure. Developed for this purpose, fold recognition methods use elaborate strategies to exploit evolutionary information, mainly by encoding amino acid sequence into profiles. Since protein structure is more conserved than sequence, the inclusion of structural information c ...
... and proteins of known structure. Developed for this purpose, fold recognition methods use elaborate strategies to exploit evolutionary information, mainly by encoding amino acid sequence into profiles. Since protein structure is more conserved than sequence, the inclusion of structural information c ...
Design and Evolution of Artificial M13 Coat Proteins
... selective pressure for increased display of heterologous fusions). These results suggest that the components of a viral phage coat need not be restricted to the gene products of the viral genome. Thus, our ®ndings support the hypothesis that, for viruses that assemble at the host membrane, membrane ...
... selective pressure for increased display of heterologous fusions). These results suggest that the components of a viral phage coat need not be restricted to the gene products of the viral genome. Thus, our ®ndings support the hypothesis that, for viruses that assemble at the host membrane, membrane ...
Identification of Both Shared and Distinct Proteins in the Major and
... have been identified. Here, the human U11/U12 small nuclear ribonucleoprotein (snRNP), a subunit of the minor (U12-dependent) spliceosome, was isolated. Twenty U11/U12 proteins were identified, including subsets unique to the minor spliceosome or common to both spliceosomes. Common proteins include ...
... have been identified. Here, the human U11/U12 small nuclear ribonucleoprotein (snRNP), a subunit of the minor (U12-dependent) spliceosome, was isolated. Twenty U11/U12 proteins were identified, including subsets unique to the minor spliceosome or common to both spliceosomes. Common proteins include ...
- computer science publication server
... Transitivity refers to the concept of deducing a structural similarity between proteins A and C from the existence of a third protein B , such that A and B as well as B and C are homologues, as ascertained if the sequence identity between A and B as well as that between B and C is above the aforemen ...
... Transitivity refers to the concept of deducing a structural similarity between proteins A and C from the existence of a third protein B , such that A and B as well as B and C are homologues, as ascertained if the sequence identity between A and B as well as that between B and C is above the aforemen ...
Gene Section NOL3 (nucleolar protein 3 (apoptosis repressor with CARD domain))
... The function of endogenous NOP30 is not known. Exogenous NOP30 interacts with SFRS9/SRp30C and NPM1 and may influence splicing (Stoss et al., 1999). ARC is an endogenous inhibitor of apoptosis that is unique in its ability to antagonize both the extrinsic (death receptor) and the intrinsic (mitochon ...
... The function of endogenous NOP30 is not known. Exogenous NOP30 interacts with SFRS9/SRp30C and NPM1 and may influence splicing (Stoss et al., 1999). ARC is an endogenous inhibitor of apoptosis that is unique in its ability to antagonize both the extrinsic (death receptor) and the intrinsic (mitochon ...
Critical care: Meeting protein requirements without overfeeding energy
... international guidelines. We determined protein adequacy of current feed prescription in a ‘baseline’ audit and then the effect of added protein in a pilot ‘supplementation’ audit. The primary outcome was the difference between protein prescription and the local guidelines on days 1e3. Secondary out ...
... international guidelines. We determined protein adequacy of current feed prescription in a ‘baseline’ audit and then the effect of added protein in a pilot ‘supplementation’ audit. The primary outcome was the difference between protein prescription and the local guidelines on days 1e3. Secondary out ...
Determination and Quantification of Molecular Interactions in Protein
... packed foods. The quality of the food has to be ensured during the whole life cycle, this includes transportation and storage at the retailors and customers. To provide safe foodstuffs it is necessary to protect them depending on their respective requirements against oxygen, water vapor, contaminati ...
... packed foods. The quality of the food has to be ensured during the whole life cycle, this includes transportation and storage at the retailors and customers. To provide safe foodstuffs it is necessary to protect them depending on their respective requirements against oxygen, water vapor, contaminati ...
HiFliQ Ni-NTA FPLC Columns - User Guide
... Reagent amounts given apply to IPTG-induced bacterial culture of a well-expressed protein (approximately 10-50 mg/l). Cells are lysed with a high concentration of urea, which also aids to dissolve insoluble protein aggregates. The His-tagged protein is then purified from the cleared lysate under den ...
... Reagent amounts given apply to IPTG-induced bacterial culture of a well-expressed protein (approximately 10-50 mg/l). Cells are lysed with a high concentration of urea, which also aids to dissolve insoluble protein aggregates. The His-tagged protein is then purified from the cleared lysate under den ...
Chemically Mediated Site-Specific Proteolysis. Alteration of Protein
... proteases having different substrate specificities, including trypsin, chymotrypsin, and elastase (17); moreover, it does so by a novel mechanism. Monomers of ecotin, having 142 amino acid residues, are held together by their long Cterminal strands (residues 125-142) that are arranged as a 2-strande ...
... proteases having different substrate specificities, including trypsin, chymotrypsin, and elastase (17); moreover, it does so by a novel mechanism. Monomers of ecotin, having 142 amino acid residues, are held together by their long Cterminal strands (residues 125-142) that are arranged as a 2-strande ...
Nutrient Metabolism
... phases (Table 2). Horses were interval trained twice a week and were walked at 1.5 m/s for 30 min on a mechanical walker on rest days. Standard exercise test (SET). Before conditioning, but after the dietary accommodation period, all horses performed a standard exercise test (SET-U). The SET consist ...
... phases (Table 2). Horses were interval trained twice a week and were walked at 1.5 m/s for 30 min on a mechanical walker on rest days. Standard exercise test (SET). Before conditioning, but after the dietary accommodation period, all horses performed a standard exercise test (SET-U). The SET consist ...
Protein Detection Methods in Proteomics Research
... because the proteomics approach measures quantitative changes in expression levels in biological samples. A number of very different techniques exist. Ideally the detection limit should be as low as possible with an optimal signal to noise ratio. For proper quantification of proteins in typical prote ...
... because the proteomics approach measures quantitative changes in expression levels in biological samples. A number of very different techniques exist. Ideally the detection limit should be as low as possible with an optimal signal to noise ratio. For proper quantification of proteins in typical prote ...
Nitrogen Balance and Protein Requirements: Definition and
... It has been proposed that protein requirement is, within a certain limit, inversely dependent on energy intake, i.e. the more energy is ingested, the less protein is needed (Table 4). This is because proteins can be used also as energ y sources (beyond their structural, regulatory and functional rol ...
... It has been proposed that protein requirement is, within a certain limit, inversely dependent on energy intake, i.e. the more energy is ingested, the less protein is needed (Table 4). This is because proteins can be used also as energ y sources (beyond their structural, regulatory and functional rol ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.