Sourirajan, Jayanthi: Protein Structure Prediction
... required for the cellular development, differentiation, replication and survival. The key to the wide variety of functions exhibited by the individual proteins is not its linear sequence but its three dimensional structure. The knowledge of the 3D structure is useful for rational drug design, protei ...
... required for the cellular development, differentiation, replication and survival. The key to the wide variety of functions exhibited by the individual proteins is not its linear sequence but its three dimensional structure. The knowledge of the 3D structure is useful for rational drug design, protei ...
Protein Measurement with the Folin Phenol Reagent
... It is unnecessary to bring all the samples and standards to the same volume before the addition of the alkaline copper reagent, provided corThe critical volrections are made for small differences in final volume. umes are those of the alkaline copper and Folin reagents. If the protein is present in ...
... It is unnecessary to bring all the samples and standards to the same volume before the addition of the alkaline copper reagent, provided corThe critical volrections are made for small differences in final volume. umes are those of the alkaline copper and Folin reagents. If the protein is present in ...
The Malaria Parasite`s Chloroquine Resistance Transporter is a
... PfCRT Belongs to a Family of Proteins The proteins with the greatest similarity to PfCRT are homologs from other Plasmodia species (P. vivax, P. knowlesi, P. yoelii yoelii, P. chabaudi, and P. berghei) and these are retrieved by a BlastP search with P values in the range of 102162 to 102137. The P v ...
... PfCRT Belongs to a Family of Proteins The proteins with the greatest similarity to PfCRT are homologs from other Plasmodia species (P. vivax, P. knowlesi, P. yoelii yoelii, P. chabaudi, and P. berghei) and these are retrieved by a BlastP search with P values in the range of 102162 to 102137. The P v ...
mechanism of the flagellar export system and its potential
... flagellum. As every motor, it consists of a rotor and a stator. The MS ring and the adjoining Cring make up the rotor, while the stator is formed around it from MotA-MotB complexes. The T3SS is assembled at the base of the MS ring. At the cytoplasmic face of the T3SS the so-called soluble export com ...
... flagellum. As every motor, it consists of a rotor and a stator. The MS ring and the adjoining Cring make up the rotor, while the stator is formed around it from MotA-MotB complexes. The T3SS is assembled at the base of the MS ring. At the cytoplasmic face of the T3SS the so-called soluble export com ...
Tay Sachs: A Protein Targeting Disease
... • Receptors for M6P exist in selected membranes (e.g., transGolgi Network or TGN; cell membrane) to ensure this occurs Non-MPR pathway • Pathways that use other sequences to target enzymes to lysosomes • Some lysosomal enzymes lack M6P and are targeted in different ways • The classic lysosomal enzym ...
... • Receptors for M6P exist in selected membranes (e.g., transGolgi Network or TGN; cell membrane) to ensure this occurs Non-MPR pathway • Pathways that use other sequences to target enzymes to lysosomes • Some lysosomal enzymes lack M6P and are targeted in different ways • The classic lysosomal enzym ...
Protein measurement with the Folin-phenol reagent
... It is unnecessary to bring all the samples and standards to the same volume before the addition of the alkaline copper reagent, provided corThe critical volrections are made for small differences in final volume. umes are those of the alkaline copper and Folin reagents. If the protein is present in ...
... It is unnecessary to bring all the samples and standards to the same volume before the addition of the alkaline copper reagent, provided corThe critical volrections are made for small differences in final volume. umes are those of the alkaline copper and Folin reagents. If the protein is present in ...
SISYPHUS—structural alignments for proteins with non
... decade ago, structural classification of proteins (SCOP) is a database of known structural and probable evolutionary relationships amongst proteins of known structure (1). These relationships are projected on a hierarchical tree which evolves with the increasing amount of structural data. The basic ...
... decade ago, structural classification of proteins (SCOP) is a database of known structural and probable evolutionary relationships amongst proteins of known structure (1). These relationships are projected on a hierarchical tree which evolves with the increasing amount of structural data. The basic ...
Eli Martinez poster
... reactive oxygen species (ROS). Manganese also acts as an enzyme cofactor for superoxide dismutase and other enzymes. In bacteria, manganese and Mn-SOD allow bacteria to withstand ROS created from internal and external metabolic processes. Pathogenic bacteria require manganese to remain virulent in e ...
... reactive oxygen species (ROS). Manganese also acts as an enzyme cofactor for superoxide dismutase and other enzymes. In bacteria, manganese and Mn-SOD allow bacteria to withstand ROS created from internal and external metabolic processes. Pathogenic bacteria require manganese to remain virulent in e ...
Eukaryotically Encoded and Chloroplast
... rubredoxin has a similar distribution to that of photosystem II complexes, suggested a co-localization of rubredoxin with PS II. To test if rubredoxin is attached to the photosynthesis machinery or is a part of it, we isolated PS II-enriched complexes from spinach thylakoids. One fraction consisted ...
... rubredoxin has a similar distribution to that of photosystem II complexes, suggested a co-localization of rubredoxin with PS II. To test if rubredoxin is attached to the photosynthesis machinery or is a part of it, we isolated PS II-enriched complexes from spinach thylakoids. One fraction consisted ...
us whey proteins in ready-to-drink beverages
... like the shake products, use a retort or UHT process which is a high heat treatment. Whey proteins that are not modified to have more heat stability will not be stable as the sole protein ingredient at levels above 3% protein. Unmodified whey proteins will gel or precipitate under these conditions u ...
... like the shake products, use a retort or UHT process which is a high heat treatment. Whey proteins that are not modified to have more heat stability will not be stable as the sole protein ingredient at levels above 3% protein. Unmodified whey proteins will gel or precipitate under these conditions u ...
U.S. WHEY PROTEINS IN READY-TO-DRINK BEVERAGES
... like the shake products, use a retort or UHT process which is a high heat treatment. Whey proteins that are not modified to have more heat stability will not be stable as the sole protein ingredient at levels above 3% protein. Unmodified whey proteins will gel or precipitate under these conditions u ...
... like the shake products, use a retort or UHT process which is a high heat treatment. Whey proteins that are not modified to have more heat stability will not be stable as the sole protein ingredient at levels above 3% protein. Unmodified whey proteins will gel or precipitate under these conditions u ...
How to obtain labeled proteins and what to do with... Marlon J Hinner and Kai Johnsson
... tags within cells. The specificity of biarsenical labels for their tag is not perfect; labeling and washing procedures therefore have to be carried out in presence of a competing thiol reagent to reduce background labeling. This labeling procedure – which is also slightly cytotoxic [31] – may be p ...
... tags within cells. The specificity of biarsenical labels for their tag is not perfect; labeling and washing procedures therefore have to be carried out in presence of a competing thiol reagent to reduce background labeling. This labeling procedure – which is also slightly cytotoxic [31] – may be p ...
The Strategic Use of Ruminally Protected Amino Acids in Dairy
... programs with the same nutrient composition of CP, ADICP, NDICP, NDF, ADF, fat, and ash. Program defaults are then used to calculate the predicted flow of metabolizable protein (MP), microbial protein, methionine and lysine to the small intestine. This comparison is presented in Tables 2 and 2a. Not ...
... programs with the same nutrient composition of CP, ADICP, NDICP, NDF, ADF, fat, and ash. Program defaults are then used to calculate the predicted flow of metabolizable protein (MP), microbial protein, methionine and lysine to the small intestine. This comparison is presented in Tables 2 and 2a. Not ...
Understanding the functional difference between growth
... II of functional divergence. A stringent 95% threshold of posterior probability was used. FUNDI requires a MSA without gaps (insertion or deletion). As some of the sequences contained deletions or had ambiguous residues (annotated with multiple ‘X’), 30 different MSAs were generated by removing one ...
... II of functional divergence. A stringent 95% threshold of posterior probability was used. FUNDI requires a MSA without gaps (insertion or deletion). As some of the sequences contained deletions or had ambiguous residues (annotated with multiple ‘X’), 30 different MSAs were generated by removing one ...
Advances in Natural and Applied Sciences Aedes aegypti Aulanni’am
... To counter-attack the host mechanism for inhibiting blood feeding, SG of arthropod vectors has developed molecular components i.e. vasodilators and immunomodulators. Vasodilator factors potentially inhibit homeostasis. They function as an anti-coagulants and inhibit vasoconstriction. Immunomodulator ...
... To counter-attack the host mechanism for inhibiting blood feeding, SG of arthropod vectors has developed molecular components i.e. vasodilators and immunomodulators. Vasodilator factors potentially inhibit homeostasis. They function as an anti-coagulants and inhibit vasoconstriction. Immunomodulator ...
Tubulin Subunit Carboxyl Termini Determine Polymerization Efficiency
... anesulfonyl fluoride in dimethyl sulfoxide. Assembly was monitored by turbidity a t 350 nm using a thermostated Cary Model 219 spectrophotome~r.Following digestion and Tubulin is a heterodimer whose two subunits ( a and p) addit.ion of phenylmethanesulfonyl fluoride, samples were placed on have been ...
... anesulfonyl fluoride in dimethyl sulfoxide. Assembly was monitored by turbidity a t 350 nm using a thermostated Cary Model 219 spectrophotome~r.Following digestion and Tubulin is a heterodimer whose two subunits ( a and p) addit.ion of phenylmethanesulfonyl fluoride, samples were placed on have been ...
Functional analysis of the Drosophila Dnop5 gene using targeted RNA interference
... and modification of the pre-rRNA seems to occur simultaneously with the assembly of ribosomal proteins (r-proteins) and large transient RNP (ribonucleoprotein) particles are formed in the nucleolus [1]. Different sets of proteins were found in each complex. Nop5/sik1 gene encode components of small ...
... and modification of the pre-rRNA seems to occur simultaneously with the assembly of ribosomal proteins (r-proteins) and large transient RNP (ribonucleoprotein) particles are formed in the nucleolus [1]. Different sets of proteins were found in each complex. Nop5/sik1 gene encode components of small ...
Proteins and Albumin
... respectively. Amino acid chains containing more than a dozen or so residues are often referred to as oligopeptides (from the Greek oligos, meaning “few”). Proteins are typically defined as polypeptides with a molecular weight of greater than 5000 Daltons (ie, > 5 kDa). Proteins adopt stable conforma ...
... respectively. Amino acid chains containing more than a dozen or so residues are often referred to as oligopeptides (from the Greek oligos, meaning “few”). Proteins are typically defined as polypeptides with a molecular weight of greater than 5000 Daltons (ie, > 5 kDa). Proteins adopt stable conforma ...
Sporopollenin Biosynthetic Enzymes Interact and
... produced fusions to either the GALACTOSE4 (GAL4) DNA-binding domain (BD) or activation domain (AD). After yeast transformation with different couples of AD and BD constructs, mating mixtures were plated on yeast media lacking Leu, Trp, and His to select transformed colonies wherein protein interacti ...
... produced fusions to either the GALACTOSE4 (GAL4) DNA-binding domain (BD) or activation domain (AD). After yeast transformation with different couples of AD and BD constructs, mating mixtures were plated on yeast media lacking Leu, Trp, and His to select transformed colonies wherein protein interacti ...
The phosphorylation of proteins: a major mechanism for biological
... point the three known examples of enzyme regulation by phosphorylation-dephosphorylation, phosphorylase, phosphorylase kinase and the synthase, all involved glucogen metabolism, and it was considered by some that perhaps this was an esoteric type of control system restricted t o one limited area of ...
... point the three known examples of enzyme regulation by phosphorylation-dephosphorylation, phosphorylase, phosphorylase kinase and the synthase, all involved glucogen metabolism, and it was considered by some that perhaps this was an esoteric type of control system restricted t o one limited area of ...
Explaining Gluten
... water is mixed with wheat flour. Proteins are very large molecules composed of amino acids. Two of the naturally occurring proteins in flour are called glutenin and gliadin. When sufficient water is added to dry flour the two proteins emerge from a “frozen state” and become flexible and able to move ...
... water is mixed with wheat flour. Proteins are very large molecules composed of amino acids. Two of the naturally occurring proteins in flour are called glutenin and gliadin. When sufficient water is added to dry flour the two proteins emerge from a “frozen state” and become flexible and able to move ...
extraction of keratin protein from chicken feather
... I = length of the solution light passes through (cm) c= concentration of the solution (mol dm-1) Absorbance is proportional to the concentration of a solution. So increasing the absorbance also shows higher protein concentration. It was observed that the highest absorbance is in sodium sulfide react ...
... I = length of the solution light passes through (cm) c= concentration of the solution (mol dm-1) Absorbance is proportional to the concentration of a solution. So increasing the absorbance also shows higher protein concentration. It was observed that the highest absorbance is in sodium sulfide react ...
Probability-Based Scoring Function as a Software
... results, five criteria were considered consecutively, (i) probability-based score, (ii) number of matched proteins, (iii) pI, (vi) protein molecular mass and (v) % coverage. On the output interface, all results were represented in the form of tables and lists of the amino acid sequences of theoretic ...
... results, five criteria were considered consecutively, (i) probability-based score, (ii) number of matched proteins, (iii) pI, (vi) protein molecular mass and (v) % coverage. On the output interface, all results were represented in the form of tables and lists of the amino acid sequences of theoretic ...
Challenges in characterising biopharmaceuticals for ease of
... characterisation during bioprocess development and product formulation. It also brought in academics with interests in these challenges, as well as those involved in developing biophysical and associated computational or data handling techniques, that may have a relevant impact in future. The meetin ...
... characterisation during bioprocess development and product formulation. It also brought in academics with interests in these challenges, as well as those involved in developing biophysical and associated computational or data handling techniques, that may have a relevant impact in future. The meetin ...
2.7. Future of plant-based protein sources
... Especially non-vegetarians have the perception that vegetarian food is very difficult, laborious or time-consuming to prepare. They often think that beans and soy products need hours of soaking or that the products need germinating. Even some vegetarians were frustrated with the long time that some ...
... Especially non-vegetarians have the perception that vegetarian food is very difficult, laborious or time-consuming to prepare. They often think that beans and soy products need hours of soaking or that the products need germinating. Even some vegetarians were frustrated with the long time that some ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.