Know Your Feed Terms - Alberta Agriculture and Forestry
... which can be expressed as a per cent of total dry matter or as a percentage of total nitrogen/protein. The concentration of ADIN is used to determine protein availability in heated feeds. Estimates of crude protein (CP) available to the animal can be adjusted by using the following guideline. If ADI ...
... which can be expressed as a per cent of total dry matter or as a percentage of total nitrogen/protein. The concentration of ADIN is used to determine protein availability in heated feeds. Estimates of crude protein (CP) available to the animal can be adjusted by using the following guideline. If ADI ...
Protein Metabolism and Acidosis
... degradation between control and acidotic rats. This revealed that without ATP, acid induced proteolysis did not occur. Thus, it can be concluded that acidosis stimulates muscle protein degradation by activating the adenosine triphosphate-dependent pathway involving ubiquitin and proteasomes. (Figure ...
... degradation between control and acidotic rats. This revealed that without ATP, acid induced proteolysis did not occur. Thus, it can be concluded that acidosis stimulates muscle protein degradation by activating the adenosine triphosphate-dependent pathway involving ubiquitin and proteasomes. (Figure ...
Spectrophotometric Determination of Total Protein
... Proteins are macromolecules with molar masses ranging upwards of 6000 Daltons. They are formed from colvalently bonded amino acids units. The simplest amino acid is glycine whose structure is: O ...
... Proteins are macromolecules with molar masses ranging upwards of 6000 Daltons. They are formed from colvalently bonded amino acids units. The simplest amino acid is glycine whose structure is: O ...
Biochem Review, Part I: Protein Structure and Function
... • Tertiary structure can be complex and does not typically consist of repeating units. • A polypeptide will adopt the most stable tertiary structure In aqueous environment, this occurs when hydropobic residues are internal and hydrophilic residues are external. ...
... • Tertiary structure can be complex and does not typically consist of repeating units. • A polypeptide will adopt the most stable tertiary structure In aqueous environment, this occurs when hydropobic residues are internal and hydrophilic residues are external. ...
Primary and secondary metabolism, and post
... of the Gram-positive bacterium Streptomyces coelicolor A3(2) has recently been completely sequenced and annotated (Bentley et al., 2002). At 8.67 Mb and 7825 annotated genes, it is nearly twice the size of the Escherichia coli (Blattner et al., 1997) and Bacillus subtilis (Kunst et al., 1997) genome ...
... of the Gram-positive bacterium Streptomyces coelicolor A3(2) has recently been completely sequenced and annotated (Bentley et al., 2002). At 8.67 Mb and 7825 annotated genes, it is nearly twice the size of the Escherichia coli (Blattner et al., 1997) and Bacillus subtilis (Kunst et al., 1997) genome ...
TMEM65 is a mitochondrial inner-membrane protein
... the basal ganglion (Huntsman et al., 2005). Studies have shown that LSFC is caused by mutations in the gene encoding leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) (Mootha et al., 2003). LRPPRC regulates mitochondrial mRNA stability and the deficiency of this protein results in de ...
... the basal ganglion (Huntsman et al., 2005). Studies have shown that LSFC is caused by mutations in the gene encoding leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) (Mootha et al., 2003). LRPPRC regulates mitochondrial mRNA stability and the deficiency of this protein results in de ...
dorsal - WebLearn
... As the ovarioles develop, follicle cells migrate over the oocyte and the nurse cells pump their contents into the oocyte, leaving only a remnant of the nurse cells behind at the anterior ...
... As the ovarioles develop, follicle cells migrate over the oocyte and the nurse cells pump their contents into the oocyte, leaving only a remnant of the nurse cells behind at the anterior ...
Longins and their longin domains: regulated SNAREs and
... be subdivided into Qa- (syntaxin), Qb- [25-kDa synaptosomal-associated protein (SNAP-25) N-terminal SNARE motif] or Qc- (SNAP-25 C-terminal SNARE motif) SNAREs [6]. The R-SNAREs can be also subdivided into short VAMPs (vesicle-associated membrane proteins) or ‘brevins’ (from the Latin word brevis, m ...
... be subdivided into Qa- (syntaxin), Qb- [25-kDa synaptosomal-associated protein (SNAP-25) N-terminal SNARE motif] or Qc- (SNAP-25 C-terminal SNARE motif) SNAREs [6]. The R-SNAREs can be also subdivided into short VAMPs (vesicle-associated membrane proteins) or ‘brevins’ (from the Latin word brevis, m ...
Supporting Information Heim et al. 10.1073/pnas.1413018111
... evaluation. DNA encoding the PC967 P1 derivatives, referred to herein as rP1-Cla1Upstream and rP1-Cla1Up/Downstream, was cloned into pET-30(c) and used to transform E. coli BL21 (DE3) cells. rP1, rP1-Cla1Upstream, and rP1-Cla1Up/Downstream sample preparation was performed as described above for the ...
... evaluation. DNA encoding the PC967 P1 derivatives, referred to herein as rP1-Cla1Upstream and rP1-Cla1Up/Downstream, was cloned into pET-30(c) and used to transform E. coli BL21 (DE3) cells. rP1, rP1-Cla1Upstream, and rP1-Cla1Up/Downstream sample preparation was performed as described above for the ...
DR AMENA RAHIM BIOCHEMISTRY
... Secondary derived proteins These are formed as intermediates during the ...
... Secondary derived proteins These are formed as intermediates during the ...
The Structure of Amino Acids in Proteins
... http://www.biology.arizona.edu/biochemistry/problem_sets/aa/aa.html http://www.concord.org/~barbara/homs_workbench/amino_acids1.html ...
... http://www.biology.arizona.edu/biochemistry/problem_sets/aa/aa.html http://www.concord.org/~barbara/homs_workbench/amino_acids1.html ...
Statistical analysis of DNA microarray data
... The term threading was first coined by Jones, Taylor and Thornton in 1992, and originally referred specifically to the use of a full 3-D structure atomic representation of the protein template in fold recognition. Today, the terms threading and fold recognition are frequently (though somewhat incorr ...
... The term threading was first coined by Jones, Taylor and Thornton in 1992, and originally referred specifically to the use of a full 3-D structure atomic representation of the protein template in fold recognition. Today, the terms threading and fold recognition are frequently (though somewhat incorr ...
PROTEIN SUBCELLULAR LOCALIZATION - PolyU
... be found in protein databases [8]. Over the years, a number of homology-based predictors have been proposed. For example, Proteome Analyst [9] computes the feature vectors for classification by using the presence or absence of some tokens from certain fields of the homologous sequences in the SwissP ...
... be found in protein databases [8]. Over the years, a number of homology-based predictors have been proposed. For example, Proteome Analyst [9] computes the feature vectors for classification by using the presence or absence of some tokens from certain fields of the homologous sequences in the SwissP ...
View PDF - Sutro Biopharma, Inc.
... manipulate folding and stability of the displayed protein, important properties for favorable production of biotherapeutics. For example chaperones can be added to tune proper formation of disulfide bonds in antibody fragments [38]. In a recent study, Buchanan et al. [39] were able to isolate fun ...
... manipulate folding and stability of the displayed protein, important properties for favorable production of biotherapeutics. For example chaperones can be added to tune proper formation of disulfide bonds in antibody fragments [38]. In a recent study, Buchanan et al. [39] were able to isolate fun ...
Journal of Bacteriology
... in Rhodospirillum rubrum (13, 17). The inactive Fe protein also could be activated in vitro by treatment with an activating enzyme isolated from the chromatophores of R. rubrum (29). In R. rubrum, the regulation of nitrogenase activity is mediated via the covalent modification (ADP ribosylation) of ...
... in Rhodospirillum rubrum (13, 17). The inactive Fe protein also could be activated in vitro by treatment with an activating enzyme isolated from the chromatophores of R. rubrum (29). In R. rubrum, the regulation of nitrogenase activity is mediated via the covalent modification (ADP ribosylation) of ...
Local protein synthesis in neuronal axons: why and
... neurites (which are likely to be axons) can pass through the silicone grease-culture dish interface under the Teflon divider into the “distal” compartments. With a complete seal, decent fluidic separation between compartments can be made, and axonal growth into the distal compartments is facilitated ...
... neurites (which are likely to be axons) can pass through the silicone grease-culture dish interface under the Teflon divider into the “distal” compartments. With a complete seal, decent fluidic separation between compartments can be made, and axonal growth into the distal compartments is facilitated ...
Early states during protein folding - The Astbury Centre for Structural
... folding kinetics by adding kinetic traps? In order to answer these questions, we need to be able to detect all the species populated during folding and to characterise their structural, dynamic and spectroscopic properties in as much detail, and at as high a resolution, as possible. Whilst this can ...
... folding kinetics by adding kinetic traps? In order to answer these questions, we need to be able to detect all the species populated during folding and to characterise their structural, dynamic and spectroscopic properties in as much detail, and at as high a resolution, as possible. Whilst this can ...
The database of epoxide hydrolases and
... the α/β hydrolase fold are well conserved. The cap domains are also similar in shape and size, and consist of 4–5 α-helices arranged in 2 layers. The classification of EHs based on the length of NC- and cap-loop (Barth et al., 2004) was applied to 11 homologous families. Cluster I includes soluble m ...
... the α/β hydrolase fold are well conserved. The cap domains are also similar in shape and size, and consist of 4–5 α-helices arranged in 2 layers. The classification of EHs based on the length of NC- and cap-loop (Barth et al., 2004) was applied to 11 homologous families. Cluster I includes soluble m ...
Lecture 7 - CS
... ab initio restricted to small (100aa), single domain proteins + information about contacts • Contact prediction from coevolution -> dramatic increase of scope (… 500aa) ...
... ab initio restricted to small (100aa), single domain proteins + information about contacts • Contact prediction from coevolution -> dramatic increase of scope (… 500aa) ...
mitochondria
... compartment to the other - vesicles become loaded with cargo molecules derived from the lumen of the compartment as they bud and pinch off from its membrane - cargo becomes discharged into a second compartment by fusing with the membrane enclosing that compartment ...
... compartment to the other - vesicles become loaded with cargo molecules derived from the lumen of the compartment as they bud and pinch off from its membrane - cargo becomes discharged into a second compartment by fusing with the membrane enclosing that compartment ...
Aspartic acid or Glutamic Acid Histidine
... adjacent to other non-polar atoms, providing stability by van der Waals as well as the hydrophobic interaction (+1 for either van der waals or hydrophobic interactions.) ii) (4 pts) If one of the interactions that you identified in part i was removed, how would the dissociation constant (KD) change? ...
... adjacent to other non-polar atoms, providing stability by van der Waals as well as the hydrophobic interaction (+1 for either van der waals or hydrophobic interactions.) ii) (4 pts) If one of the interactions that you identified in part i was removed, how would the dissociation constant (KD) change? ...
... predicted to be located in a small, and, based upon the model, tight loop between β-strands 8 and 9 of the N-terminus of FoxRperi (Fig. 2C). Mutational analysis of potential active site residues of PaFoxR. To identify potential active site residues responsible for the self-cleavage of FoxRperi, we f ...
Metabolic Adaptation and Protein Complexes in Prokaryotes
... predicted previously based on bioinformatic approaches from genome sequence or genetic data. Furthermore, looking in detail at both data sets ([3,18]) shows that many important interactions are conserved in both bacteria. The question of conservation of prokaryotic protein complexes and their intera ...
... predicted previously based on bioinformatic approaches from genome sequence or genetic data. Furthermore, looking in detail at both data sets ([3,18]) shows that many important interactions are conserved in both bacteria. The question of conservation of prokaryotic protein complexes and their intera ...
Leukaemia Section t(10;16)(q22;p13) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... encodes the zinc fingers, two nuclear localization signals (NLS1 and NLS2), the HAT domain, and a portion of the acidic domain, and most of the CBP protein, including its HAT domain. Oncogenesis MYST4 has a 60% identity and 66% similarity to MYST3. All the fusions involving this genes result in seve ...
... encodes the zinc fingers, two nuclear localization signals (NLS1 and NLS2), the HAT domain, and a portion of the acidic domain, and most of the CBP protein, including its HAT domain. Oncogenesis MYST4 has a 60% identity and 66% similarity to MYST3. All the fusions involving this genes result in seve ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.