A novel approach for protein subcellular location prediction using
... the protein as a proxy for location based on the hypothesis that the physicochemical properties of the residues of a protein must be somehow coupled to the physicochemical properties of the environment where the protein performs its function; therefore the differences between environments will be im ...
... the protein as a proxy for location based on the hypothesis that the physicochemical properties of the residues of a protein must be somehow coupled to the physicochemical properties of the environment where the protein performs its function; therefore the differences between environments will be im ...
nutrient composition of dandelions and its potential as human food
... die every year due to hunger. Expansion of present agriculture practices into marginal land is not expected to solve the problem of increasing the food supply. New methods of feeding the ever increasing world population must be developed. The aim of the study was to evaluate the usefulness of the da ...
... die every year due to hunger. Expansion of present agriculture practices into marginal land is not expected to solve the problem of increasing the food supply. New methods of feeding the ever increasing world population must be developed. The aim of the study was to evaluate the usefulness of the da ...
Comment in GO: This term is intended to
... Existing Terminology in GO & PRO macromolecular complex (GO:0032991) – A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together. protein complex (GO:0043234) – Any macromolecular complex composed of two ...
... Existing Terminology in GO & PRO macromolecular complex (GO:0032991) – A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together. protein complex (GO:0043234) – Any macromolecular complex composed of two ...
294_2005_38_MOESM43_ESM - Springer Static Content Server
... Sln1: All species investigated have at least one, in many instances several, histidine kinases (see also (Catlett et al. 2003)). To classify as an Sln1 orthologue, the protein had to possess two predicted N-terminal transmembrane domains in addition to the histidine kinase and response regulator dom ...
... Sln1: All species investigated have at least one, in many instances several, histidine kinases (see also (Catlett et al. 2003)). To classify as an Sln1 orthologue, the protein had to possess two predicted N-terminal transmembrane domains in addition to the histidine kinase and response regulator dom ...
Towards an Analysis of the Rice Mitochondrial Proteome
... 1996; Werhahn and Braun, 2002). A different solution to the exclusion problem, with an even wider applicability, is non-gel-based separation of complex mixtures. These approaches often use liquid chromatography (LC)-based separations of peptide derived from whole-sample digests that are directly cou ...
... 1996; Werhahn and Braun, 2002). A different solution to the exclusion problem, with an even wider applicability, is non-gel-based separation of complex mixtures. These approaches often use liquid chromatography (LC)-based separations of peptide derived from whole-sample digests that are directly cou ...
Protein digestion in poultry – the value of an
... • Protein/amino acids are among the most expensive nutrients to deliver in poultry nutrition • The digestibility of protein in poultry is typically incomplete by the terminal ileum • Undigested protein that leaves the ileum is from both exogenous (diet) and endogenous (bird) sources • Understanding ...
... • Protein/amino acids are among the most expensive nutrients to deliver in poultry nutrition • The digestibility of protein in poultry is typically incomplete by the terminal ileum • Undigested protein that leaves the ileum is from both exogenous (diet) and endogenous (bird) sources • Understanding ...
Seq_pattern_II
... not yet in plants. This gene is involved in an interesting biological process (e.g., apoptosis). You are interested in finding the orthologous gene in Arabidopsis. However, BLAST search using each of the known sequences failed to identify an Arabidopsis homologue. What else can you try? ...
... not yet in plants. This gene is involved in an interesting biological process (e.g., apoptosis). You are interested in finding the orthologous gene in Arabidopsis. However, BLAST search using each of the known sequences failed to identify an Arabidopsis homologue. What else can you try? ...
Synaptonemal complex proteins - Journal of Cell Science
... fusion proteins (accession number L32978; Moens et al., 1992) proved that these represent overlapping cDNAs of the hamster homologue of the rat SCP1 gene that encodes a 125 kDa SC protein (Meuwissen et al., 1992). The hamster and rat proteins are 90% identical. We therefore adopt for Syn1 the number ...
... fusion proteins (accession number L32978; Moens et al., 1992) proved that these represent overlapping cDNAs of the hamster homologue of the rat SCP1 gene that encodes a 125 kDa SC protein (Meuwissen et al., 1992). The hamster and rat proteins are 90% identical. We therefore adopt for Syn1 the number ...
Proteins * Structure and Function
... • The arrangement of the atoms in some molecules is such that one end of the molecule has a positive electrical charge and the other side has a negative charge. If this is the case, the molecule is called a polar molecule, meaning that it has electrical poles. Otherwise, it is called a non-polar mol ...
... • The arrangement of the atoms in some molecules is such that one end of the molecule has a positive electrical charge and the other side has a negative charge. If this is the case, the molecule is called a polar molecule, meaning that it has electrical poles. Otherwise, it is called a non-polar mol ...
Sporopollenin biosynthetic enzymes interact and constitute a
... one fluorescent protein, the donor, is transferred to another fluorescent protein, the acceptor. Since efficient energy transfer can only occur if distance between fluorophores is less than 10 nm, FRET can be used to monitor protein-protein interactions in living cells (Sun et al., 2012). To that ai ...
... one fluorescent protein, the donor, is transferred to another fluorescent protein, the acceptor. Since efficient energy transfer can only occur if distance between fluorophores is less than 10 nm, FRET can be used to monitor protein-protein interactions in living cells (Sun et al., 2012). To that ai ...
Abstract
... Among all of the transition metal ions present in all domains of life, zinc (formally Zn(II)) is one of the most widespread, reflecting the utilization of Zn(II) by proteins for a wide variety of biological functions. The vast majority of known zinc domains, such as the zinc fingers and catalytic do ...
... Among all of the transition metal ions present in all domains of life, zinc (formally Zn(II)) is one of the most widespread, reflecting the utilization of Zn(II) by proteins for a wide variety of biological functions. The vast majority of known zinc domains, such as the zinc fingers and catalytic do ...
Actin - WordPress.com
... What is G-actin? o Globular Actin, which assembles to form F-Actin What is F-actin? o Fibrous Actin, made from G-actin combining There are two types of filaments made from F-actin. What are they? o Microfilaments o Thin Filaments What are the important functions of Actin? o Cell Surface Shape o Musc ...
... What is G-actin? o Globular Actin, which assembles to form F-Actin What is F-actin? o Fibrous Actin, made from G-actin combining There are two types of filaments made from F-actin. What are they? o Microfilaments o Thin Filaments What are the important functions of Actin? o Cell Surface Shape o Musc ...
Is β-pleated sheet the molecular conformation which dictates
... experimental data, where individual prediction methods usually fail to produce meaningful results (Hamodrakas et al., 1982a; Hamodrakas and Kafatos, 1984; Hamodrakas, 1992). 2.2. Cuticular proteins The 27 proteins chosen for inclusion in this analysis are a subset of 36 unique insect cuticular prote ...
... experimental data, where individual prediction methods usually fail to produce meaningful results (Hamodrakas et al., 1982a; Hamodrakas and Kafatos, 1984; Hamodrakas, 1992). 2.2. Cuticular proteins The 27 proteins chosen for inclusion in this analysis are a subset of 36 unique insect cuticular prote ...
A typical hemoglobin molecule consists of a "heme" part and a
... occupied by a nitrogen atom from a histidine side chain on one of the amino acids in the globin. The last coordination site is available to bind an O2 molecule. The heme is therefore the oxygen-carrying portion of the hemoglobin molecules. This raises the question: What is the function of the globul ...
... occupied by a nitrogen atom from a histidine side chain on one of the amino acids in the globin. The last coordination site is available to bind an O2 molecule. The heme is therefore the oxygen-carrying portion of the hemoglobin molecules. This raises the question: What is the function of the globul ...
Domain structure and sequence similarities in cartilage proteoglycan
... protein, taken together for all four species determined, show a similarity of about 48%, while that of the B’ loops is 35%. For link protein alone, comparison among different species shows the amino acid sequence similarity between these two loops to be almost 60%. The G1 domain has been shown to bi ...
... protein, taken together for all four species determined, show a similarity of about 48%, while that of the B’ loops is 35%. For link protein alone, comparison among different species shows the amino acid sequence similarity between these two loops to be almost 60%. The G1 domain has been shown to bi ...
View/Open
... Swine: Distiller’s dried grain by-products can be used as a protein/energy source in growing pigs and reproducing sows at limited inclusion rates. v DDGS contain approximately 27 percent crude protein, but are low in lysine and to a lesser extent threonine, leucine, isoleucine, and tryptophan v DDG ...
... Swine: Distiller’s dried grain by-products can be used as a protein/energy source in growing pigs and reproducing sows at limited inclusion rates. v DDGS contain approximately 27 percent crude protein, but are low in lysine and to a lesser extent threonine, leucine, isoleucine, and tryptophan v DDG ...
et al - The EMBO Journal
... cells. In contrast, in meiocytes only a C-terminal truncated RBR protein is present, due to aberrant RBR mRNA splicing in these specialized cells. Accordingly, homozygous rbr-2 mutant plants displayed only some minor vegetative phenotypic aberrations, but a strong reduction in fertility (1–10% of wi ...
... cells. In contrast, in meiocytes only a C-terminal truncated RBR protein is present, due to aberrant RBR mRNA splicing in these specialized cells. Accordingly, homozygous rbr-2 mutant plants displayed only some minor vegetative phenotypic aberrations, but a strong reduction in fertility (1–10% of wi ...
Proteomic analysis of the signaling pathway mediated by the
... Background: The heterotrimeric Gα protein Pga1-mediated signaling pathway regulates the entire developmental program in Penicillium chrysogenum, from spore germination to the formation of conidia. In addition it participates in the regulation of penicillin biosynthesis. We aimed to advance the unde ...
... Background: The heterotrimeric Gα protein Pga1-mediated signaling pathway regulates the entire developmental program in Penicillium chrysogenum, from spore germination to the formation of conidia. In addition it participates in the regulation of penicillin biosynthesis. We aimed to advance the unde ...
The Right Whey Protein
... Each particular method of extraction is crucial to the performance and activity of each specific whey peptide, which explains why none of these advanced extraction methods is necessarily superior to the other. For example, ion-exchanged whey protein is rich in high molecular weight immunoglobulins a ...
... Each particular method of extraction is crucial to the performance and activity of each specific whey peptide, which explains why none of these advanced extraction methods is necessarily superior to the other. For example, ion-exchanged whey protein is rich in high molecular weight immunoglobulins a ...
Electromagnetic Properties of Biomolecules
... interactions between proteins and their targets (other proteins, DNA regulatory segments or small molecules). Each of these processes involves an energy transfer between the interacting molecules. These interactions are highly selective, and this selectivity is defined within the protein primary str ...
... interactions between proteins and their targets (other proteins, DNA regulatory segments or small molecules). Each of these processes involves an energy transfer between the interacting molecules. These interactions are highly selective, and this selectivity is defined within the protein primary str ...
Structure of the Coat Protein-binding Domain of
... Electrostatic interactions are somewhat nonspeci®c, especially when they do not involve a single pair of oppositely charged residues. However, this type of interaction, active over long distances, would serve well for the initial recognition and binding events. In order to lock the scaffolding and c ...
... Electrostatic interactions are somewhat nonspeci®c, especially when they do not involve a single pair of oppositely charged residues. However, this type of interaction, active over long distances, would serve well for the initial recognition and binding events. In order to lock the scaffolding and c ...
The Purification and Characterization of the Highly Labeled
... crystallin. Differences in electrophoretic mobility were also observed. The relationship between the HL protein and alpha crystallin and the basis for the observed differences between these proteins is discussed. ...
... crystallin. Differences in electrophoretic mobility were also observed. The relationship between the HL protein and alpha crystallin and the basis for the observed differences between these proteins is discussed. ...
Mass spectrometry and the search for moonlighting proteins
... quite dramatically during evolution, with the addition or subtraction of entire helices, loop regions, and pockets. Because those surface features are all potential sites for a novel protein/ protein interaction function to evolve, it is quite possible that the random accumulation of mutations on PG ...
... quite dramatically during evolution, with the addition or subtraction of entire helices, loop regions, and pockets. Because those surface features are all potential sites for a novel protein/ protein interaction function to evolve, it is quite possible that the random accumulation of mutations on PG ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.