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Gene Section CASC5 (cancer susceptibility candidate 5) Atlas of Genetics and Cytogenetics
Gene Section CASC5 (cancer susceptibility candidate 5) Atlas of Genetics and Cytogenetics

... organs, such as placenta. At least two alternative isoforms of cDNA were identified. Analysis on cancer cell lines, such as HeLa, gave single band with 8,5 kb. There is another alternative splicing site at the 5' side of this gene that generates a short exon with 78 bp in cDNA. There are potential o ...
Amino Acid Requirements and Post-absorptive Metabolism in Cattle
Amino Acid Requirements and Post-absorptive Metabolism in Cattle

... The last route assessed by Swanson (1977) was MFP, for which it is not easy to have a clear description. For example, the definition of MFP has changed with time, from “a residue of body secretions and tissue incident to movement of food through the gastrointestinal tract” (Swanson, 1977) to the de ...
The Production, Purification, and Bioactivity of Recombinant Bovine
The Production, Purification, and Bioactivity of Recombinant Bovine

... acid interferon-a that has a role in maternal recognition of pregnancy in cattle. Here we describe production of bTP-1 by recombinant procedures in Escherichia coli. A bTP-1 gene was constructed which lacked the codons representing the signal sequence and provided a Met initiation codon ahead of the ...
Prediction of Nickel Binding Sites in Proteins from Amino acid
Prediction of Nickel Binding Sites in Proteins from Amino acid

... 333 proteins of known metal-binding domains as metal-binding. These suggest the usefulness of SVM for facilitating the prediction of metal-binding proteins1. Structural information also has been used for predicting metal-binding sites based on the detection of principal liganding residues and metall ...
Nitrogen catabolite repressible GAP1 promoter, a new tool for
Nitrogen catabolite repressible GAP1 promoter, a new tool for

... from expressing cells can be lower than for other yeasts (such as Pichia pastoris), the relative amount of protein of interest versus the total biomass can result in better purification yield and purity [12]. Here we developed a novel promoter system in S. cerevisiae aiming at producing large quanti ...
Degradation signals within both terminal domains of the cauliflower
Degradation signals within both terminal domains of the cauliflower

... icosahedral symmetry (Cheng et al., 1992). The capsid is built from three related proteins: p44, p39 and p37. These are derived from bi-terminal processing of the 57 kDa CP precursor, the product of ORF IV. Both terminal domains of the CP precursor are very acidic (Figure 1), and the function of the ...
AminoMax Updates.
AminoMax Updates.

... C fraction) is more concentrated in the RUP fractions. The RUP fraction of protein consists of bypass protein that can be absorbed, as well as indigestible material that cannot be absorbed. Processes that improve RUP of proteins often result in increased indigestible protein, with some loss of the b ...
Effect of Structural Changes in Proteins Derived from GATA4
Effect of Structural Changes in Proteins Derived from GATA4

... The present study was conducted at Genomics Laboratory, Department of Studies in Zoology, University of Mysore, Karnataka, India between September 2007 and March 2013. Secondary structure analysis: Forty nine nsSNPs of GATA4 cited in literature was used in this study, of which 11 nsSNPs reported fro ...
The Carboxyl-Terminal Region of Protein C Is
The Carboxyl-Terminal Region of Protein C Is

... to the Golgi apparatus is blocked and that these mutant proteins are retained in the rough ER, whereas PC409 was transported through the Golgi apparatus and secreted normally. Molecular modeling and MD calculation of the catalytic domain of two truncated protein C mutants. To distinguish the structu ...
Protein Sentezi
Protein Sentezi

... All non-cytoplasmic proteins must be translocated • The leader peptide retards the folding of the protein so that molecular chaperone proteins can interact with it and direct its folding • The leader peptide also provides recognition signals for the translocation machinery • A leader peptidase remov ...
1. Amino acids. Of all data abstractions in
1. Amino acids. Of all data abstractions in

... structure, function, active sites, even catalytic mechanism, this empirical fact can generate useful hypotheses about how function of one protein might be inferred from the relatedness to another. The assumption is difficult to test and involves comparing how many aspects other than structure and se ...
as a PDF - CiteSeerX
as a PDF - CiteSeerX

... Recent experiments have provided evidence for both the structural and functional independence of these two domains of EIIMtl. The C-terminal domain is important for the phosphorylation function of the protein and contains at least two sites of covalent phosphorylation: His-554, the phospho acceptor ...
FEEDING YOUR PET IGUANA
FEEDING YOUR PET IGUANA

... It is recommended by many veterinarians to LIGHTLY sprinkle all the food offered to the iguana with a calcium powder (calcium gluconate, lactate, or carbonate). A LIGHT weekly sprinkling of a good reptile vitamin on the food is also recommended. A common problem seen in pet iguanas is over-supplemen ...
PDF - World Wide Journals
PDF - World Wide Journals

... fibers in the solid form found in feathers. These reagents will break down disulphide bonds, hydrogen bonds and salt linkages of the keratin fibers in order to dissolve it into protein solution. Currently there is an increasing interest in the development of materials that are environment friendly, ...
Table S4: Domains present in a network region characterized by
Table S4: Domains present in a network region characterized by

... This protein is functionally uncharacterized. It is about 300-500 amino acids in length. This family is found in plants and bacteria. This family of proteins are functionally uncharacterised. This protein is found in bacteria and eukaryotes. Proteins in this family are typically between 205 to 258 a ...
Purification and proteomic characterization of plastids from Brassica
Purification and proteomic characterization of plastids from Brassica

... © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim ...
Dietary plant-protein substitution affects hepatic metabolism in
Dietary plant-protein substitution affects hepatic metabolism in

... The high dietary protein requirements of salmonid fish are met with fishmeal-based feed in commercial aquaculture. The sustainability of this practice is questionable and, therefore, the feasibility of substituting fishmeal with plant-based products needs to be investigated. We investigated growth a ...
Finding the small difference: A nine amino acid extension to
Finding the small difference: A nine amino acid extension to

... Hepatitis B core protein can be expressed in EL coli and forms icosahedrally organised shells. Predominantly large shells consisting of 120 dimers are formed, if the C-terminus is truncated at amino acid 149. Figure 1 shows a micrograph of C-terminally truncated hepatitis B core protein. Similar she ...
What is function
What is function

... Gene Ontology - flexibility • Imagine • protein 1 phosphorylates protein 2 • protein 2 binds to protein 3 (which then binds to DNA) • proteins 1, 2, or 3 may be coded on nearby genes • makes sense in terms of regulation / protein production • different metabolic functions • part of same "cellular p ...
Amino Acid Sequence and Antigenicity of the Amino
Amino Acid Sequence and Antigenicity of the Amino

... Antigenically active regions of proteins usually contained polar residues which are located on the surface of the native molecule (Walter et al., 1980). The sequence described here contained a combination of hydrophobic and hydrophilic amino acids and its immunogenicity could not be predicted. There ...
NFX1-123 Increases HTERT Post-Transcriptionally in HPV 16E6
NFX1-123 Increases HTERT Post-Transcriptionally in HPV 16E6

... catalytic subunit of telomerase. Full activation of hTERT by 16E6 in keratinocytes (HFKs) requires the endogenous protein NFX1-123. NFX1-123 contains a PAM2 motif, to which cytoplasmic poly(A) binding proteins (PABPCs) bind, and an R3H domain, to which singlestranded nucleic acids putatively can bin ...
Electrophoresis Chapter 10 +
Electrophoresis Chapter 10 +

... •The first dimension, IEF, is carried out in a rod-shaped gel, d=1-2 mm. •8M Urea and non-ionic detergent. •The gel is incubated with an SDS-containing buffer. •Separation in the second dimension is performed by putting the strip on top of the stacking gel. •High resolution 1000-2000 (10000) protein ...
Environmentally Sensitive Fluorescent Sensors Based on Synthetic Peptides Linköping University Post Print
Environmentally Sensitive Fluorescent Sensors Based on Synthetic Peptides Linköping University Post Print

... 2.1. Applications of Peptide Biosensors Protein chips are expected to become important tools for direct analyses of biomolecular function and interaction in a high-throughput fashion. Hisakazu Mihara and his team contribute to this development by designing protein-detection systems, where peptide-ba ...
Use of infrared and visible light radiation as modulator of protein
Use of infrared and visible light radiation as modulator of protein

... frequency and the mean value over the whole spectrum. The presence of a peak ...
CS790 – Introduction to Bioinformatics
CS790 – Introduction to Bioinformatics

... protein. If each residue can take only 3 positions, there are 3100 = 5  1047 possible conformations. • If it takes 10-13s to convert from 1 structure to another, exhaustive search would take 1.6  1027 years! ...
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Bimolecular fluorescence complementation



Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.
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