Sequence
... all, the signal-to-noise ratio is greatly improved for the specific purpose of identifying protein relatives. It is accepted that convergence phenomena in aa sequences are very rare and thus aa similarity almost always means homology. Furthermore, aa sequences may still show a similarity derived fro ...
... all, the signal-to-noise ratio is greatly improved for the specific purpose of identifying protein relatives. It is accepted that convergence phenomena in aa sequences are very rare and thus aa similarity almost always means homology. Furthermore, aa sequences may still show a similarity derived fro ...
Denaturation of hemoglobin in the presence of tannic acid
... and is found in numerous plants and foods. It generally can be extracted from grape seeds. TA is known as an effective antioxidant, antimicrobial/antiviral agent, which binds to blood proteins [5–8]. The binding of a ligand to HSA and HHb can stabilize or destabilize the protein structure. Fluoresce ...
... and is found in numerous plants and foods. It generally can be extracted from grape seeds. TA is known as an effective antioxidant, antimicrobial/antiviral agent, which binds to blood proteins [5–8]. The binding of a ligand to HSA and HHb can stabilize or destabilize the protein structure. Fluoresce ...
Bioinformatics in Brief This week: DB for structures Structure
... Homologous Superfamily, HThis level groups together protein domains which are thought to share a common ancestor and can therefore be described as homologous. Similarities are identified first by sequence comparisons and subsequently by structure comparison using SSAP. the criteria: Sequence identit ...
... Homologous Superfamily, HThis level groups together protein domains which are thought to share a common ancestor and can therefore be described as homologous. Similarities are identified first by sequence comparisons and subsequently by structure comparison using SSAP. the criteria: Sequence identit ...
pGADT7 AD Vector Information
... pGADT7 AD is the AD Cloning Vector provided in Clontech's Matchmaker™ Gold Yeast One- and Two-Hybrid Screening Systems (Cat. Nos. 630491 and 630489, respectively). The vector allows the generation of GAL4 AD fusion proteins from either a gene of interest or a cDNA library. Important: Genes must be c ...
... pGADT7 AD is the AD Cloning Vector provided in Clontech's Matchmaker™ Gold Yeast One- and Two-Hybrid Screening Systems (Cat. Nos. 630491 and 630489, respectively). The vector allows the generation of GAL4 AD fusion proteins from either a gene of interest or a cDNA library. Important: Genes must be c ...
Interaction interfaces of protein domains are not topologically
... Multimeric proteins are found in every cellular location, including cell organelles, the cytosol, and cell membranes. Interactions between different proteins are critical in all known cellular processes, such as signaling and transcriptional regulation, metabolism of small and large molecules, prote ...
... Multimeric proteins are found in every cellular location, including cell organelles, the cytosol, and cell membranes. Interactions between different proteins are critical in all known cellular processes, such as signaling and transcriptional regulation, metabolism of small and large molecules, prote ...
The cloning and overexpression of E coli acyl carrier protein (ACP)
... The similarity of E. coli ACP to many plant ACPs is such that E. coli ACP can be used as a substitute for plant ACP in many assays for plant fatty acid synthetase activity; indeed it sometimes proves more active in vitro than the native plant ACP [3]. This is useful because such large quantities of ...
... The similarity of E. coli ACP to many plant ACPs is such that E. coli ACP can be used as a substitute for plant ACP in many assays for plant fatty acid synthetase activity; indeed it sometimes proves more active in vitro than the native plant ACP [3]. This is useful because such large quantities of ...
The Proteins of the Keratin Component of Bird`s Beaks
... pleated sheet structure very similar to that found in feather could be observed for the hard portions of beak and lizard claw; this structure is not found in native mammalian keratins. These data suggest that mammalian and non-mammalian keratins do not share common structural components. An examinat ...
... pleated sheet structure very similar to that found in feather could be observed for the hard portions of beak and lizard claw; this structure is not found in native mammalian keratins. These data suggest that mammalian and non-mammalian keratins do not share common structural components. An examinat ...
Silk-inspired polymers and proteins
... spiders (ADF-3 and ADF-4) that contain β-sheet-forming blocks of Ala6 or Ala8 and are known as eADF-3 and eADF-4 respectively [24]. Our proteins (molecular masses between 46 and 106 kDa) can be produced in high yield by high-density fermentation in E. coli (or other suitable hosts such as yeast) and ...
... spiders (ADF-3 and ADF-4) that contain β-sheet-forming blocks of Ala6 or Ala8 and are known as eADF-3 and eADF-4 respectively [24]. Our proteins (molecular masses between 46 and 106 kDa) can be produced in high yield by high-density fermentation in E. coli (or other suitable hosts such as yeast) and ...
EVIDENCE FOR TWO DISTINCT CLASSES OF STREPTOCOCCAL
... conserved domain on their surface give a positive serum opacity reaction, whereas nearly all isolates possessing the conserved domain fail to produce OF. Based on these fundamental differences, we propose that most group A streptococcal serotypes fall into one of two major classes of M protein, whic ...
... conserved domain on their surface give a positive serum opacity reaction, whereas nearly all isolates possessing the conserved domain fail to produce OF. Based on these fundamental differences, we propose that most group A streptococcal serotypes fall into one of two major classes of M protein, whic ...
Chapter Five - DORAS
... mM glycerol and 100 µg/ml carbenicillin (section 2.14.1). Glycerol was used as a complementary carbon source while carbenicillin was selected for its higher stability compared to ampicillin. Cultures of 50 ml were inoculated with 1 ml of a stationary phase overnight culture. For each strain, an indu ...
... mM glycerol and 100 µg/ml carbenicillin (section 2.14.1). Glycerol was used as a complementary carbon source while carbenicillin was selected for its higher stability compared to ampicillin. Cultures of 50 ml were inoculated with 1 ml of a stationary phase overnight culture. For each strain, an indu ...
Overview of Rule Curation
... Curate name and some references Optional: write abstract indicating function, structure, etc. ...
... Curate name and some references Optional: write abstract indicating function, structure, etc. ...
Protein folding
... from the ER and has been subjected to oligosaccharide modifications in the Golgi apparatus. To determine whether the mature form of G544V-mutant LDLR appeared at the cell surface, confocal laser microscopy on intact cells was performed. The wild-type LDLR was present on the plasma membrane, whereas ...
... from the ER and has been subjected to oligosaccharide modifications in the Golgi apparatus. To determine whether the mature form of G544V-mutant LDLR appeared at the cell surface, confocal laser microscopy on intact cells was performed. The wild-type LDLR was present on the plasma membrane, whereas ...
Modified Strains PJ69-7A and PJ69-7B for the Yeast Two
... The yeast two-hybrid system is a powerful technique used to detect protein-protein interactions. The technique has been used to establish physical interactions between genetically identified proteins, to identify the components of multiprotein complexes, and to map specific domains within proteins r ...
... The yeast two-hybrid system is a powerful technique used to detect protein-protein interactions. The technique has been used to establish physical interactions between genetically identified proteins, to identify the components of multiprotein complexes, and to map specific domains within proteins r ...
See Source - Pentelute Lab
... SrtA (SrtA*) that had improved enzymatic kinetics. 26 Once we confirmed SrtA* and SrtA gave similar product yield in model peptide studies, we chose to work exclusively with SrtA* because it substantially shortened reaction time. Reactions with SrtA* were complete in 30 minutes as opposed to 2 hours ...
... SrtA (SrtA*) that had improved enzymatic kinetics. 26 Once we confirmed SrtA* and SrtA gave similar product yield in model peptide studies, we chose to work exclusively with SrtA* because it substantially shortened reaction time. Reactions with SrtA* were complete in 30 minutes as opposed to 2 hours ...
BCA Assay
... method is not a true end-point method; that is, the final color continues to develop. However, following incubation, the rate of continued color development is sufficiently slow to allow large numbers of samples to be assayed together. The macromolecular structure of protein, the number of peptide b ...
... method is not a true end-point method; that is, the final color continues to develop. However, following incubation, the rate of continued color development is sufficiently slow to allow large numbers of samples to be assayed together. The macromolecular structure of protein, the number of peptide b ...
HOW TO USE BAD: A BRIEF PRESENTATION
... together with the .NET framework (VB.NET) was used to write the BAD web project. The whole application is composed of several *.aspx files with the names corresponding to the adequate protein adsorption components. Each web page contains a dynamically loaded table that presents the results of query ...
... together with the .NET framework (VB.NET) was used to write the BAD web project. The whole application is composed of several *.aspx files with the names corresponding to the adequate protein adsorption components. Each web page contains a dynamically loaded table that presents the results of query ...
Bone Morphogenetic protein-4 active
... Introduction: The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the TGF-beta superfamily which includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bon ...
... Introduction: The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the TGF-beta superfamily which includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bon ...
Protease Activity of a 90-kDa Protein Isolated from Scallop Shells
... acquired on an Ultraflex tandem time-of-flight (TOF/TOF) instrument (Bruker Daltonics, MA, U.S.A) in the positive reflection mode. The TOF/TOF spectra of casein fragments was measured, data were processed by flex analysis and BioToolsTM software (Bruker Daltonics), and each fragment was assigned usi ...
... acquired on an Ultraflex tandem time-of-flight (TOF/TOF) instrument (Bruker Daltonics, MA, U.S.A) in the positive reflection mode. The TOF/TOF spectra of casein fragments was measured, data were processed by flex analysis and BioToolsTM software (Bruker Daltonics), and each fragment was assigned usi ...
Protein Posttranslational Modifications: The Chemistry of Proteome
... modifications. Enzymes dedicated to such protein modifications include 500 human protein kinases, 150 protein phosphatases, and 500 proteases. The major types of protein covalent modifications, such as phosphorylation, acetylation, glycosylation, methylation, and ubiquitylation, can be classified ac ...
... modifications. Enzymes dedicated to such protein modifications include 500 human protein kinases, 150 protein phosphatases, and 500 proteases. The major types of protein covalent modifications, such as phosphorylation, acetylation, glycosylation, methylation, and ubiquitylation, can be classified ac ...
domain alignments - Oxford Academic
... proteins in Entrez. CDART, which stores that information, can be invoked from within Entrez to visualize domain architectures (6). Proteins in Entrez can now be neighboured by similar domain architecture, in addition to sequence similarity as detected by BLAST (7). Conserved Domains are neighboured ...
... proteins in Entrez. CDART, which stores that information, can be invoked from within Entrez to visualize domain architectures (6). Proteins in Entrez can now be neighboured by similar domain architecture, in addition to sequence similarity as detected by BLAST (7). Conserved Domains are neighboured ...
Identification of a family of BspA like surface proteins of Entamoeba
... Our examination of the E. histolytica genome revealed multiple putative genes encoding proteins with leucine rich repeat (LRR) motifs that resemble BspA-like proteins. Here, we describe the cloning, sequencing, and expression of one of the genes encoding an E. histolytica LRR containing protein, (Eh ...
... Our examination of the E. histolytica genome revealed multiple putative genes encoding proteins with leucine rich repeat (LRR) motifs that resemble BspA-like proteins. Here, we describe the cloning, sequencing, and expression of one of the genes encoding an E. histolytica LRR containing protein, (Eh ...
BMC Bioinformatics
... promoters on the chromosome and two endogenous plasmids. Although the promoter search is an efficient method for identifying effectors, it has several limitations. (a) Not all genes preceded by the hrp promoters encode effectors. (b) Some effector genes may not have a hrp promoter. Therefore, the hr ...
... promoters on the chromosome and two endogenous plasmids. Although the promoter search is an efficient method for identifying effectors, it has several limitations. (a) Not all genes preceded by the hrp promoters encode effectors. (b) Some effector genes may not have a hrp promoter. Therefore, the hr ...
Characterisation and functional properties of watermelon (Citrullus
... form of rind and seeds. The seeds are utilised directly for human consumption in various forms, such as snacks in India, Arabian and African countries, as an additive in various dishes, for decorating cakes and as a stuffing in indigenous kheer.2,3 The kernels are used in sweetmeats and toppings as ...
... form of rind and seeds. The seeds are utilised directly for human consumption in various forms, such as snacks in India, Arabian and African countries, as an additive in various dishes, for decorating cakes and as a stuffing in indigenous kheer.2,3 The kernels are used in sweetmeats and toppings as ...
Bimolecular fluorescence complementation
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex. Therefore, through the visualisation and analysis of the intensity and distribution of fluorescence in these cells, one can identify both the location and interaction partners of proteins of interest.