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What kind of movie is this (give your best guess)?
A) A C. elegans embryo expressing GFP
B) A C. elegans embryos expressing a GFP fusion that is
asymmetrically distributed to different blastomeres
A) A C. elegans embryos expressing a PIE-1 GFP fusion that is
asymmetrically distributed to different blastomeres during cell
divisions
B) A C. elegans embryos expressing a GFP fusion that shows a
punctate staining pattern
C) A C. elegans embryos expressing a GFP fusion that always
goes to the germ cellss
What is the major issue of this paper?
A) How C. elegans embryos divide?
B) How germline specification is achieved?
C) What is PIE-1 protein?
D) Where is pie-1 mRNA is localized?
E) How the EMS blastomere arise?
How did the authors clone the pie-1 gene?
A) Through Antibody staining
B) Tc1 transposon insertion or tagging
C) C. elegans lineage analysis
D) They cloned the gene by pure luck
E) They cloned the gene by a candidate screen
What is the most important concept that you
learn from reading this paper?
A.
The localization patterns of PIE-1
B.
How pluripotency of a stem cell is maintained by a general repressor
C.
What kind of protein is PIE-1
D.
C. elegans is a good animal model for studying embryo development
E.
PIE-1 associates with centrosomes during cell division
How did the authors reach the conclusion that PIE-1
might be a transcriptional repressor?
A. from antibody staining
B. from cell lineage analysis
C. from protein sequence
D. it is just a wild guess
E. from its interaction with other somatic-determining genes
What are the important questions that the paper did not
address and need to be analyzed in subsequent study?
A. Whether PIE-1 is a transcription repressor
B.
How is PIE-1 selectively degraded in somatic daughters
C.
How does PIE-1 associate with centrosomes
D.
What are the repression targets of PIE-1
E.
All of the above