CLS 1113 Introduction to Clinical Laboratory Practices
... • Enzymes are naturally occurring molecules that catalyze specific biochemical reactions. • They react with suitable substances to produce products that are chromogenic (color), fluorogenic, or luminescent. ...
... • Enzymes are naturally occurring molecules that catalyze specific biochemical reactions. • They react with suitable substances to produce products that are chromogenic (color), fluorogenic, or luminescent. ...
• Warm-up What are the four macromolecules and their function?
... • reactions proceed slowly below optimal temperatures • above 45 C most enzymes are denatured (change in their shape so the enzyme active site no longer fits with the substrate and the enzyme can't function) ...
... • reactions proceed slowly below optimal temperatures • above 45 C most enzymes are denatured (change in their shape so the enzyme active site no longer fits with the substrate and the enzyme can't function) ...
Chapter Eight: Enzymes: Basic Concepts and Kinetics
... 29. Why would you expect thrombin to have greater substrate specificity than trypsin? Answer: Thrombin carries out a specific reaction in blood clotting. Trypsin is responsible for the digestion of proteins in the digestive system, and must be able to cleave thousands of different proteins. Thus, i ...
... 29. Why would you expect thrombin to have greater substrate specificity than trypsin? Answer: Thrombin carries out a specific reaction in blood clotting. Trypsin is responsible for the digestion of proteins in the digestive system, and must be able to cleave thousands of different proteins. Thus, i ...
Voet Chapter 12 Slides
... To experimentally determine the rate constant of a second-order reaction A + B -> P, it is often convenient to increase the concentration of one reactant relative to the other, [B] >> [A]; under these conditions, [B] does not change significantly over the course of the reaction. The reaction rate t ...
... To experimentally determine the rate constant of a second-order reaction A + B -> P, it is often convenient to increase the concentration of one reactant relative to the other, [B] >> [A]; under these conditions, [B] does not change significantly over the course of the reaction. The reaction rate t ...
enzyme kinetics
... substrate affinity for the enzyme: it is numerically equal to the concentration of [S] at V = 0.5 Vmax. In general, a lower value of Km means tighter substrate binding. In fact, for those cases where kcat is much smaller than k–1, the Km will be equal to Kd, the dissociation constant for substrate b ...
... substrate affinity for the enzyme: it is numerically equal to the concentration of [S] at V = 0.5 Vmax. In general, a lower value of Km means tighter substrate binding. In fact, for those cases where kcat is much smaller than k–1, the Km will be equal to Kd, the dissociation constant for substrate b ...
Catalytic Strategies
... binding between enzyme and substrate involves an induced fit mechanism? • What catalytic strategy is used by this ...
... binding between enzyme and substrate involves an induced fit mechanism? • What catalytic strategy is used by this ...
Biology Knowledge Organiser Topic 3: Threshold Concepts in Biology
... This comes about due to the specific shape of the active site: only one molecule (according to its shape) will fit into the active site. See diagram for an illustration. Enzymes have an optimum temperature and pH. If the temperature is too high (for most enzymes, above about 45oC), or the pH is too ...
... This comes about due to the specific shape of the active site: only one molecule (according to its shape) will fit into the active site. See diagram for an illustration. Enzymes have an optimum temperature and pH. If the temperature is too high (for most enzymes, above about 45oC), or the pH is too ...
Oxidation of fatty acids
... Beta - Oxidation • 4 enzyme catalyzed reactions take place 1) Dehydrogenation of Fatty acyl CoA producing double bond. 2) Addition of H2O to the double bond to form Lstereoisomer of Beta-Hydroxyacyl CoA. 3) Dehydrogenation to Beta ketoacyl CoA. 4) Thiolysis by thiloase catalyzed reaction to give of ...
... Beta - Oxidation • 4 enzyme catalyzed reactions take place 1) Dehydrogenation of Fatty acyl CoA producing double bond. 2) Addition of H2O to the double bond to form Lstereoisomer of Beta-Hydroxyacyl CoA. 3) Dehydrogenation to Beta ketoacyl CoA. 4) Thiolysis by thiloase catalyzed reaction to give of ...
Amino Acids Proteins, and Enzymes
... • are most active at optimum pH. • contain R groups of amino acids with proper charges at optimum pH. • lose activity in low or high pH as tertiary structure is disrupted. • body have an optimum pH of about 7.4. ...
... • are most active at optimum pH. • contain R groups of amino acids with proper charges at optimum pH. • lose activity in low or high pH as tertiary structure is disrupted. • body have an optimum pH of about 7.4. ...
Pre-‐lab AP Lab: Enzyme Catalysis Name
... In the absence of catalase, this reaction occurs spontaneously, but very slowly. Catalase speeds up the reaction considerably. In this experiment, a rate for this reaction will be determined. ...
... In the absence of catalase, this reaction occurs spontaneously, but very slowly. Catalase speeds up the reaction considerably. In this experiment, a rate for this reaction will be determined. ...
Enzyme Inhibition
... activity of all the active sites on the enzyme Animation: “ An example of allosteric activation of an ...
... activity of all the active sites on the enzyme Animation: “ An example of allosteric activation of an ...
Some Review Questions for the Chapter 2 and 3
... 42. Why are enzymes needed in biological systems? What do they do for a reaction? What kind of substance are enzymes? 43. On what part of the enzyme does its substrate attach to? Why are enzymes so specific for their substrate? 44. What kinds of conditions can change the activity of an enzyme? 45. W ...
... 42. Why are enzymes needed in biological systems? What do they do for a reaction? What kind of substance are enzymes? 43. On what part of the enzyme does its substrate attach to? Why are enzymes so specific for their substrate? 44. What kinds of conditions can change the activity of an enzyme? 45. W ...
... catalyze reactions at high rates under mild conditions. Enzymatic catalysis can have many advantages over conventional catalytic processes, namely less energy consumption and fewer unwanted by-products. However, a natural enzyme that can catalyze the desired reaction does not exist for most indus ...
ENZYMES - Bio12.com
... The respiration pathway accelerates and ATP (the final product) builds up in the cell As the ATP increases, more and more ATP fits into the allosteric site of the phosphofructokinase molecules The enzyme’s conformation changes again and stops accepting substrate molecules in the active site Respirat ...
... The respiration pathway accelerates and ATP (the final product) builds up in the cell As the ATP increases, more and more ATP fits into the allosteric site of the phosphofructokinase molecules The enzyme’s conformation changes again and stops accepting substrate molecules in the active site Respirat ...
J. Anim. Sci. Vol. 80, Suppl. 1/J. Dairy Sci. Vol. 85, Suppl. 1 414 Use
... the diet. When viewed across a variety of enzyme products and experimental conditions the response to feed enzymes by ruminants has been variable. This variation can be attributed to experimental conditions in which energy is not the limiting nutrient, as well as the activities and characteristics o ...
... the diet. When viewed across a variety of enzyme products and experimental conditions the response to feed enzymes by ruminants has been variable. This variation can be attributed to experimental conditions in which energy is not the limiting nutrient, as well as the activities and characteristics o ...
Catalase Lab How do enzymes work in living tissues? Introduction
... enzymes to break down these poisonous chemicals into harmless substances. Enzymes are proteins that speed up the rate of reactions that would otherwise happen more slowly. The enzyme is not altered by the reaction. You have hundreds of different enzymes in each of your cells. Each of these enzymes i ...
... enzymes to break down these poisonous chemicals into harmless substances. Enzymes are proteins that speed up the rate of reactions that would otherwise happen more slowly. The enzyme is not altered by the reaction. You have hundreds of different enzymes in each of your cells. Each of these enzymes i ...
Event Poster PDF
... The role of induced-fit in enzyme specificity has been a controversial topic for the past 50 years (1-3). Structural studies often reveal a change in enzyme structure from an open to a closed state after substrate binding, which could be explained by the need for an open site to bind substrate, but ...
... The role of induced-fit in enzyme specificity has been a controversial topic for the past 50 years (1-3). Structural studies often reveal a change in enzyme structure from an open to a closed state after substrate binding, which could be explained by the need for an open site to bind substrate, but ...
Study Guide for Chemistry of Life Test
... Be able to explain that proteins are made of chains of amino acids and that there are 20 types of amino acids in proteins Be able to explain give examples of some of the roles of different proteins in living things: structural support, enzymes, transport molecules etc. Be able to define activation ...
... Be able to explain that proteins are made of chains of amino acids and that there are 20 types of amino acids in proteins Be able to explain give examples of some of the roles of different proteins in living things: structural support, enzymes, transport molecules etc. Be able to define activation ...
Biology - WordPress.com
... 6. Define denaturation of proteins:___________________________________ 7. Name 3 things that can denature or unfold an enzyme. _________ ______________________ ______________________ 8. In this lab, what weak acid denatured the protein? _______________ 9. What was the purpose of placing one test tub ...
... 6. Define denaturation of proteins:___________________________________ 7. Name 3 things that can denature or unfold an enzyme. _________ ______________________ ______________________ 8. In this lab, what weak acid denatured the protein? _______________ 9. What was the purpose of placing one test tub ...
ENZYME
... -Amylase is used for the production of malt, as the enzyme is produced during the germination of cereal grains Checking out pancerititis the amylase levels are measured in the pancertic cells. ...
... -Amylase is used for the production of malt, as the enzyme is produced during the germination of cereal grains Checking out pancerititis the amylase levels are measured in the pancertic cells. ...
Catalysts in biochemical reactions
... Enzyme is a class of proteins that function as catalysts in biochemical reactions. On their Characteristics is that they increases the rate of reactions by several orders of magnitude A very dramatic example of enzyme kinetics is given by decomposition of hydrogen Peroxide. Enzymes are usually prote ...
... Enzyme is a class of proteins that function as catalysts in biochemical reactions. On their Characteristics is that they increases the rate of reactions by several orders of magnitude A very dramatic example of enzyme kinetics is given by decomposition of hydrogen Peroxide. Enzymes are usually prote ...
Effect of Catalase Concentration on the
... Enzymes are proteins that serve as biological catalysts in a wide variety of lifesustaining chemical reactions that take place in cells. As catalysts, enzymes lower the amount of energy required to make a reaction occur. We call this energy the activation energy. By lowering the activation energy, e ...
... Enzymes are proteins that serve as biological catalysts in a wide variety of lifesustaining chemical reactions that take place in cells. As catalysts, enzymes lower the amount of energy required to make a reaction occur. We call this energy the activation energy. By lowering the activation energy, e ...
Catalase and Hydrogen Peroxide
... Enzymes are proteins that serve as biological catalysts in a wide variety of lifesustaining chem-ical reactions that take place in cells. As catalysts, enzymes lower the amount of energy required to make a reaction occur. We call this energy the activation energy. By lowering the activation energy, ...
... Enzymes are proteins that serve as biological catalysts in a wide variety of lifesustaining chem-ical reactions that take place in cells. As catalysts, enzymes lower the amount of energy required to make a reaction occur. We call this energy the activation energy. By lowering the activation energy, ...
Open file
... from the active site of the enzyme and the enzyme can be used again. By increasing the substrate concentration in the experiment, the enzyme is more ...
... from the active site of the enzyme and the enzyme can be used again. By increasing the substrate concentration in the experiment, the enzyme is more ...
Isomerase
Isomerases are a general class of enzymes which convert a molecule from one isomer to another. Isomerases can either facilitate intramolecular rearrangements in which bonds are broken and formed or they can catalyze conformational changes. The general form of such a reaction is as follows:A–B → B–AThere is only one substrate yielding one product. This product has the same molecular formula as the substrate but differs in bond connectivity or spatial arrangements. Isomerases catalyze reactions across many biological processes, such as in glycolysis and carbohydrate metabolism.