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Chem331 Lect 14 Membranes
Chem331 Lect 14 Membranes

... Single Channel Pores: Made up of dimer, trimer, etc. multimeric protein subunits : Multimeric subunit assemblies where each subunit has its own pore: Facilitated Diffusion Membrane Channel Proteins Channels are often selective for a particular type of ion or molecule. ...
Prokaryotic Annotation at TIGR
Prokaryotic Annotation at TIGR

... to the functions and processes in which they were involved. with the first prokaryotic genome published, H. influenzae, we adapted Monica Riley’s E. coli role categories We have continued to modify that role scheme and still assign TIGR roles today In 1998 we recognized that it would be really usefu ...
Chapter 3: Enzymes: Structure and Function
Chapter 3: Enzymes: Structure and Function

Structural Biology: What does 3D tell us?
Structural Biology: What does 3D tell us?

... arose from one another or from a common ancestor. In looking at the amino acid sequences, sometimes there are obvious homologies, and you could predict that the 3-D structures would be similar. But sometimes virtually identical 3-D structures have no sequence similarities at all! ...
Mitochondrial Uncoupling Proteins in Mammals and Plants
Mitochondrial Uncoupling Proteins in Mammals and Plants

... SfUCPb does not contain this signature. However, alignment of both SfUCPs showed their very identity; the only difference is that 35 amino acids of SfUCPa (position 204–238) are missing in SfUCPb. The third signature in SfUCPa starts in position Pro235, four amino acids before end of deletion in SfU ...
Amino Acids
Amino Acids

Genetics RNA and Protein Synthesis
Genetics RNA and Protein Synthesis

30_General pathways of amino acids transformation
30_General pathways of amino acids transformation

... resynthesis of proteins Half-lives of proteins – from several minutes to many years Structural proteins – usually stable (lens protein crystallin lives during the whole life of the organism) Regulatory proteins - short lived (altering the amounts of these proteins can rapidly change the rate of meta ...
Three-Dimensional Structure of Adenosylcobinamide Kinase
Three-Dimensional Structure of Adenosylcobinamide Kinase

... the site for guanylylation is most likely a histidine (10). Since CobU requires GTP for the transferase activity and can utilize either ATP or GTP for the kinase activity, presumably there must be two separate binding sites for these nucleotides and hence two active sites. Thus CobU presents several ...
Active site amino acid sequence of the bovine O6
Active site amino acid sequence of the bovine O6

... expression of a transfected E. coli ada+ gene encoding an m6-Gua repair function (7-9). Thus, m6-Gua is strongly implicated not only in the mutagenic, but also in the cytotoxic and clastogenic action of agents such as MNNG towards mammalian cells. In many bacteria (including E. coli (10), M. luteus ...
Curriculum Vitae
Curriculum Vitae

... molecular mechanisms of hormone signal transduction and transcriptional regulatory complexes by adapting diverse molecular, biochemical, cellular and genetic techniques. Anthocyanins, a class of plant secondary metabolites distributed ubiquitously in the plant kingdom, endow plants with a colorful w ...
"Hydrophobic Interactions in Proteins". In: Encyclopedia of Life
"Hydrophobic Interactions in Proteins". In: Encyclopedia of Life

... with a smaller one. Table 3 summarizes the results of a series of experiments of this type. If we consider, for example, the leucine-to-alanine substitutions included in the table, the average loss in stability is 14.6 + 4.6 kJ mol 2 1. As can be seen, however, there is a very large spread in the in ...
Structural Genomics - University of Houston
Structural Genomics - University of Houston

... Contact Distance ...
Biology is the only subject in which multiplication is the same thing
Biology is the only subject in which multiplication is the same thing

... gene codes for a protein channel that allows salt to flow across cell membrane  broken protein doesn’t work as channel  doesn’t allow salt out of cell, so water doesn’t flow out ...
Davisco Whey Protein Processing
Davisco Whey Protein Processing

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... 12. What is meant by lactose intolerance? How it can be overcome? 13. How the viscosity of starch solution is related to its structure? 14. Give a brief account of cyclodextrins and their use. 15. Explain the biological role of NAD+. 16. What are (i) oxidoreductases (ii) transferases. Give two examp ...
IB104 - Lecture 7 - Molecules of life
IB104 - Lecture 7 - Molecules of life

... covalently linked amino acids. Note that there is a polarity to proteins; they always start with an amino group and end with a carboxy group. These are sometimes abbreviated as the N- and C-termini of proteins. ...
Big Biology meets Obvious
Big Biology meets Obvious

... another simple linear code is the basis of life ...
Gene Section IGFBP6 (insulin-like growth factor binding protein 6)
Gene Section IGFBP6 (insulin-like growth factor binding protein 6)

... 2005; Bach et al., 2013). At least some of its actions in regulating cell fate are mediated by interaction with Ku80, a DNA-end binding protein (Iosef et al., 2010). IGFBP-6 has also been reported to have IGF-independent actions, such as promotion of cancer cell migration an IGFindependent mechanism ...
Molecular Dynamics Simulations of Protein
Molecular Dynamics Simulations of Protein

... minimises bacterial contamination Cells do not adhere directly to implanted surfaces, but instead bind to proteins in the extracellular matrix (ECM) via integrin receptors ...
Sequencing genomes
Sequencing genomes

... A new species of frog has been introduced into an area where it has too few natural predators. In an attempt to restore the ecological balance, a team of scientists is considering introducing a species of bird which feeds on this frog. Experimental data suggests that the population of frogs and bird ...
Document
Document

... The individual polypeptide chains that make up a multimeric protein are often referred to as protein subunits. Subunits are joined by ionic, H and hydrophobic interactions ...
Powerpoint slides - School of Engineering and Applied Science
Powerpoint slides - School of Engineering and Applied Science

... The individual polypeptide chains that make up a multimeric protein are often referred to as protein subunits. Subunits are joined by ionic, H and hydrophobic interactions ...
Proteins
Proteins

... Electrophoresis is usually performed on serum rather than plasma since the fibrinogen present in plasma produces a band in the β region that might be mistaken for a paraprotein. ...
Figure 5-2
Figure 5-2

... 10. A molecule that has long chains of hydrocarbons is probably a: a. Carbohydrate c. Lipid b. Protein d. Nucleic acid 11. Glucose and galactose have the same chemical formula (C6H12O6) but different structural arrangements. Glucose and galactose are: a. Isotopes c. Polymers b. Isomers d. Disacchar ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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