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Fab Four – The Muscle-Building Supplements
Fab Four – The Muscle-Building Supplements

... Dr. Robert G. Silverman graduated Magna cum Laude from the University of Bridgeport, College of Chiropractic. He is a Certified Nutrition Specialist, Certified Clinical Nutritionist, has a Masters of Science in Human Nutrition, is a Certified Strength and Conditioning Specialist, and is a Diplomat w ...
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PROTEIN CHEMISTRY
PROTEIN CHEMISTRY

... characterised by absence of a key protein, as it has been recognised as dysfunctional and eliminated by the cell’s own machinery. Diseases caused as a consequence of misfolding, include cystic fibrosis & other disease .  In addition, some cancers may be associated with misfolding. Many protein misf ...
Drug Target Discovery by Genome Analysis
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... Local vs global comparisons Summary • Proteins are organised into domains • Local comparisons find short stretches of similarity • Global comparisons match the whole length of one sequence against another • Local comparisons should be used unless sequences are closely related and have identical dom ...
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Chapter 3 Problem Set
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... easy way to do this. You prepare a pure 50 M solution of the protein, and you place it in a sample cell with a 1-cm path length, and you measure the absorbance of this sample at 280 nm in a UV-visible spectrophotometer. The absorbance of the solution is 0.372. Are there tyrosines in this protein? Ho ...
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... – Substrate binds to enzyme at active site – Enzymes act on substrates to reduce energy needed to make product – Substrate is changed – Enzyme separates from products and can form an association with another substrate – Enzyme, as a catalyst is not used up in the reaction – Increases reaction rate ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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