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Sup2 - Postech
Sup2 - Postech

... Figure S3. Conformational differences between the two PfNurA subunits. The M domains of the two PfNurA subunits exhibit significant structural differences and one monomer should be rotated and translated by at least 20 Å to be approximately overlayed with another PfNurA subunit. ...
Some Biology that Computer Scientists Need for
Some Biology that Computer Scientists Need for

... Related Metabolic Processes ...
Recombinant Human Interferon Omaga-1 (rh IFNW1)
Recombinant Human Interferon Omaga-1 (rh IFNW1)

... freeze-thaw cycles. Purity: Greater than 95.0% as determined by HPLC and SDS-PAGE. Amino Acid Sequence: MCDLPQNHGL LSRNTLVLLH QMRRISPFLC LKDRRDFRFP QEMVKGSQLQ KAHVMSVLHE MLQQIFSLFH TERSSAAWNM TLLDQLHTGL HQQLQHLETC LLQVVGEGES AGAISSPALT LRRYFQGIRV YLKEKKYSDC AWEVVRMEIM KSLFLSTNMQ ERLRSKDRDL GSS. Biol ...
Chapter 3: Organic Molecules
Chapter 3: Organic Molecules

... Carbon is the central atom in all organic molecules.  Can bond to one another, creating long chains or rings.  Carbon likes to have ______ covalent bonds.  When several carbon are present, they form the backbone to which other elements or molecules attach. ...
The Two Major Membrane Skeletal Proteins (Articulins) of Euglena
The Two Major Membrane Skeletal Proteins (Articulins) of Euglena

... skeleton, and the microtubules found in vivo (Leedale, 1964; Mignot, 1965; Dubreuil and Bouck, 1985) are not required for in vitro assembly of the membrane skeleton. To determine the relationship of the articulins to one another and to other cytoskeletal proteins, we have selected clones encoding ar ...
Long Noncoding RNAs Add Another Layer to Pre
Long Noncoding RNAs Add Another Layer to Pre

... Loci encoding long ncRNAs often overlap with or are interspersed between multiple protein-coding or noncoding genes in the genome, where they may regulate the expression of their neighbors. Moreover, long ncRNAs have been shown to act as chromatin modifiers, as transcriptional regulators that affect ...
Correlating mRNA and protein Abundance
Correlating mRNA and protein Abundance

Nitrogen Metabolism
Nitrogen Metabolism

... What are the nonessential amino acids synthesized from? • Their synthesis depends on the availability of the appropriate carbon skeletons and a source of ammonia. • Glucose is ultimately the source of carbon skeletons for most nonessential aa. • Two essential aa, phenylalanine and methionine, are u ...
Document
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... • N-formylmethionine in prokaryotes is _______________ • specific bonds in precursors are cleaved, as for example, preproinsulin to proinsulin to insulin • ___________ _________are removed by specific proteases of the endoplasmic reticulum; the Golgi apparatus then directs the finished protein to it ...
Function
Function

... Homologous proteins may have diverged in sequence and function (leave aside recruitment) Assume no strong sequence similarity to protein of known function ...
Document
Document

... The GO Consortium produces three ontologies covering the concepts that could be described as: •Molecular Function: elemental activity or task: DNA binding •Biological Process: broad objective or goal: mitosis, signal transduction. •Cellular Component: location or complex: nucleus, ribosome ...
Molecular Biology Databases
Molecular Biology Databases

... terminal domain fusion protein. * BINDS BINDS BINDS
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A1980JB88800001

... awaken much interest at a time when the role of transfer RNAs as ‘adaptor molecules’ was not recognized. Among those present at this seminar were Gabriel de la Haba and a visitor, Edward Reich. “It was de la Haba who, two years later, encouraged me to take my proposal seriously We had both returned ...
HUMANIZATION N-GLYCOSYLATION PATHWAY PLANTS PLANT
HUMANIZATION N-GLYCOSYLATION PATHWAY PLANTS PLANT

... the base-pairing between the two antiparallel DNA strains is lost. Because of this, A-, C-, G- and U-ribonucleotides (monomers!) can now base pair with the DNA template. The RNA polymerase is able to carry out the polymerisation reaction by connecting single nucleotides (aligned along the DNA strand ...
Presentation
Presentation

... in this pocket to ensure tight binding We also looked for other potential binding sites in nearby amino acids ...
18.2 Protein Structure and Function: An Overview
18.2 Protein Structure and Function: An Overview

Protein structure-function relationship: Recognition
Protein structure-function relationship: Recognition

... producing Fab and Fc occur? NOTE: the different relative position of Fab and Fc here compared to slide 5. This variation is common among antibody molecules. ...
Bacterial Structures Capsule or Glycocalyx FLAGELLA TYPES OF
Bacterial Structures Capsule or Glycocalyx FLAGELLA TYPES OF

... altered. (eg. Phosphate is added to glucose and phosphorylated glucose cannot be transported back out of cell) • Proton motive force: High concentration of H+ outside of membrane accumulates during metabolism and can be used to transport substances. ...
CPP1
CPP1

... protein of POR 1 (CPP1), an essential protein for chloroplast development, plays a role in the regulation of POR stability and function. CPP1 contains a J-like domain and three transmembrane domains and is localized in the thylakoid and envelope membranes, and interacts with POR isoforms in chloropl ...
Structural Bioinformatics - LCQB
Structural Bioinformatics - LCQB

... •  Algorithm in structural bioinformatics can help predict and classify protein structures, describe their motions and interactions, design new ...
Laboratory Exercise #7: Column Chromatography of GFP proteins
Laboratory Exercise #7: Column Chromatography of GFP proteins

... – i.e. transformed – with a pGLO plasmid that contains a gene for the Green Fluorescent Protein (GFP) from the jellyfish Aequoria victoria. As the bacterial cells express proteins from their endogenous genome, they will also express the GFP protein from the GFP gene within the pGLO plasmid. You have ...
Biochemistry
Biochemistry

... Gene expression: transcription (including defects) Gene expression: translation (including defects) Structure and function of proteins Energy metabolism Metabolic pathways of small molecules Biology of cells Human development and genetics Biology of tissue response to disease Nutrition Pharmacodynam ...
PDBe Motif
PDBe Motif

... • Pair bonds – interactions statistics between a pair of residues (Ligandaminoacid, ligand-ligand, ligand-nucleic acid etc.) • Motif binding – search for sequence pattern –distribute against ligands, secondary structures, 3D motifs • Search – combine different sources of data generate your query • U ...
CLONING A LYSINE-RICH PROTEIN GENE FROM POTATO
CLONING A LYSINE-RICH PROTEIN GENE FROM POTATO

... ABSTRACT: Lysine is one of the limiting essential amino acids because it is not synthesized in the body of animals and human. They must obtain lysine from their diet. Recent results of gene transfer research showed the possibility of overexpression of genes encoding natural lysine-rich proteins in c ...
Quality Components of Feeds
Quality Components of Feeds

... Proteins are a group of compounds which contain nitrogen. If you know the nitrogen content of a feed, then you can easily determine crude protein, because on average, the mean nitrogen content of crude protein is 16%. A minimum of 15% protein is required to maintain milk production in lactating dair ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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