• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Name:______________________________
Name:______________________________

... a) of the formation of ordered benzene molecules around the non-polar sidechains. b) of the inability to reform hydrogen bonds with the solvent. Each h-bond broken and not reformed is 25kJ/mol! Very unfavourable. c) of the formation of strong van der Waals interactions with the solvent. d) reduction ...
Document
Document

... Some proteins may undergo proteolytic processing. The well-known example is the proteolytic processing of polyprotein POMC. ...
Chapter 22, Proteins
Chapter 22, Proteins

... bond lie in the same plane. ¾The C=O and N-H groups of peptide bonds from adjacent chains point toward each other and are in the same plane so that hydrogen bonding is possible between them. ¾All R-groups on any one chain alternate, first above, then below the plane of the sheet, etc. ¾The distincti ...
Biomolecules Cut n Paste Slides
Biomolecules Cut n Paste Slides

... called nucleotides. Nucleotides consist of three parts: a 5-carbon sugar; a phosphate group; and a nitrogenous base. Nucleic acids store and transmit hereditary or genetic information. There are two kinds of nucleic acids: ribonucleic acid (RNA) which is single stranded and deoxyribonucleic acid (DN ...
Complementary spectroscopic techniques for protein X-ray
Complementary spectroscopic techniques for protein X-ray

DNA to Protein Overview
DNA to Protein Overview

... Diagram showing the translation of mRNA and the synthesis of proteins by a ribosome [Image courtesy of LadyofHats] During protein translation amino acids are linked together to form a polypeptide chain which will later be folded into a protein. The ribosome is the workbench or factory for protein tr ...
sequence - Université d`Ottawa
sequence - Université d`Ottawa

... for mammalian nuclear DNA (regions not under functional constraint) ~ 4 x 10 -9 nt sub per site per year ...
biologically important molecules
biologically important molecules

... Because CELLULOSE has alternating glucose orientation, we do not have an enzyme that can break it down. So we cannot eat grass for energy like cows (ruminants) who have the enzyme CELLULASE due to microorganisms in their guts that create it. ...
No Slide Title
No Slide Title

... http://bmb-itservices.bmb.psu.edu/bryant/lab/Project/Hydrogen/index.html#secti ...
Biochemistry
Biochemistry

... made of one type of atom. • Chemical symbols are used to represent each atom. – Found on the Periodic Table ...
1. Given the molecule: a. What type of molecule is this? b. Give the
1. Given the molecule: a. What type of molecule is this? b. Give the

... g. Would you expect concentrations of this type of molecule to be higher in animals living in the arctic or in the tropics? Explain why in 40 words or fewer. 5. Multiple choice. Which one of the following statements is correct? a. Transbilayer diffusion of lipids is slow because of the flexibility o ...
Role of the ubiquitinselective CDC48UFD1/NPL4 chaperone
Role of the ubiquitinselective CDC48UFD1/NPL4 chaperone

... oleic (18:1) acids by the introduction of a single double bond between carbon 9 and carbon 10 of the respective carbon chains (Mitchell and Martin, 1995). Yeast cells are not able to synthesize polyunsaturated fatty acids, but they can take up these compounds from the environment and incorporate the ...
Food biomolecules
Food biomolecules

... 73. True or False. Glucose is a monosaccharide.  74. True or False. Nitrogen is a trace element.  75. True or False. Eggs are a good source of fat in the diet.  76. True or False. All vitamins are fat soluble.  77. True or False. Lipids are made of amino acids.  78. True or False. Iodine turns starc ...
Crystallization Laboratory - UCLA
Crystallization Laboratory - UCLA

... diffraction pattern slightly. Comparing diffraction patterns from native and derivative data sets gives phase information. ...
Machine Learning Designs for Artificial Histone Acetyltransferases
Machine Learning Designs for Artificial Histone Acetyltransferases

Hybrid tryptophan aporepressor containing ligand binding sites
Hybrid tryptophan aporepressor containing ligand binding sites

... AIDS patients (Y archoan et al., J. Clin. Invest, 78:439 factors, interleukins, interferons, and components of the (1986)). At the least, a substantial fraction of AIDS complement system (O'Garra et al., Immunol. Today, 50 patients have chronic EBV infections. Exposure of 8:45 (1988); Weigle et al., ...
Genomes 3/e - Illinois Institute of Technology
Genomes 3/e - Illinois Institute of Technology

... of transcription initiation Primary regulation occurs at the level of transcription initiation & decides which gene is expressed in a particular cell & relative rate Secondary regulation is during the posttranscription (e.g. mRNA modification) and the protein synthesis & modification. Figure 11.22 G ...
chapter 13 section 2 notes
chapter 13 section 2 notes

Chapter 3 Review Questions
Chapter 3 Review Questions

... 14. Fruits, vegetables, cereal, and pasta contain ______________________. 15. Lipids could be found in these foods: _____________________________. 16. Amino acids are linked by ___________________. 17. The ________ group attached to an amino acid determines what kind of amino acid is present of the ...
Chapter 5: What are the major types of organic molecules?
Chapter 5: What are the major types of organic molecules?

... tertiary structure (3) of a protein is the overall folded shape of a single polypeptide chain, determined by secondary structure combined with interactions between R groups (NOTE: book defines this in a confusing way, use my way) ...
How Do You Clone a Gene?
How Do You Clone a Gene?

... DNA. The process of inserting a new gene into a DNA molecule is called cloning. The newly-constructed DNA molecule and the cell in which it is found is called a clone. The plasmid molecule in the cell replicates, making many more like itself. The cell will also divide many times and a large number o ...
Lecture 3
Lecture 3

... – Enzymes are proteins that speed chemical reactions. – Transporter proteins move chemicals across membranes. – Structural or mechanical functions, that maintain cell shape ( actin, cell wall). – Cell signaling - complex system of communication that coordinates cell actions. – Flagella are made of p ...
Identification and Characterization of the Potato Leafroll Virus
Identification and Characterization of the Potato Leafroll Virus

... The start codons used for the 23K ORF and 17K ORF are the first A U G codons following a stop codon ('k) in the same frame. ...
fae04be7f127386
fae04be7f127386

... that play a role in protein targeting by making use of protein engineering. If we join the stretch of nucleotides that codes for the endoplasmic reticulum signal sequence to the cDNA that codes for a cytosolic protein, we produce a chimeric DNA ...
Preparation of Escherichia coli</Emphasis
Preparation of Escherichia coli

< 1 ... 281 282 283 284 285 286 287 288 289 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report