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Organic Compounds
Organic Compounds

... Enzymes are a type of protein that speed up chemical reactions. They are known as "biological catalysts." For example, your stomach would not be able to break down food if it did not have special enzymes to speed up the rate of digestion. Antibodies that protect you against disease are proteins. Mus ...
Biomolecule Reading
Biomolecule Reading

... chemical reaction can start. By lowering the activation energy of chemical reactions, enzymes help cells build and break down complex molecules like starches and proteins. Much like the other biomolecules, an enzymes shape determines its function. Enzymes will only function if they have a specific m ...
Molekul - Universiti Kebangsaan Malaysia
Molekul - Universiti Kebangsaan Malaysia

... molecular structure of molecules such as DNA, RNA, protein and the function of compartments in living cells • The knowledge in molecular biology enables manipulations to be done for example in biotechnology • Implications: CGAT, BioValley, Human Genome Project ...
1) Which residues prefer helix, strand, turn:
1) Which residues prefer helix, strand, turn:

... Trp: Very hydrophobic; biggest amino acid and therefore hard to remove or insert by mutagenesis and by evolution, and thus the most conserved of all residues. Met: Start codon, and thus often positively charged on its backbone N because of which it is found at the protein surface than its hydrophobi ...
Structure, expression and phylogenetic analysis of the glycoprotein
Structure, expression and phylogenetic analysis of the glycoprotein

Monoclonal Antibody To Human GPR50
Monoclonal Antibody To Human GPR50

... from date of despatch. Aliquots of stock solution can be kept frozen at -20°C for two years from date of reconstitution; do not freeze working dilutions. optimal dilution should be tested by serial dilution. 5g/ml (1:50) Formalin / Paraffin (pretreatment with microwave required, see protocol under ...
No Slide Title
No Slide Title

... Important records in SwissProt (3) Features section: post-translational modifications, signal peptides, binding sites, enzyme active sites, domains, disulfide bridges, local secondary structure, sequence conflicts between references etc. etc. ...
Gene Section PTPN6 (protein tyrosine phosphatase, non- receptor type 6)
Gene Section PTPN6 (protein tyrosine phosphatase, non- receptor type 6)

... dermatoses appears in several clinical forms as idiopathic, tumor associated, postinfectious and drug induced (for example after an administration of granulocyte macrophage colony stimulating factor). SW and PG have strong associations with hematological tumors. Recent studies have shown that patien ...
Proteins - MATERI KULIAH PANGAN
Proteins - MATERI KULIAH PANGAN

... Electrophoresis is usually performed on serum rather than plasma since the fibrinogen present in plasma produces a band in the β region that might be mistaken for a paraprotein. M. Zaharna Clin. Chem. 2009 ...
Atomistic modeling of the structural components of the
Atomistic modeling of the structural components of the

... Olga Glukhova, Sararov State University, Russia Olga Grishina, Sararov State University, Russia Michail Slepchenkov, Sararov State University, Russia ...
The Production, Purification, and Bioactivity of Recombinant Bovine
The Production, Purification, and Bioactivity of Recombinant Bovine

... since they resemble each other in inferred amino acid sequence more than they do the related bovine IFNaM (5). There is particularly high conservation of the 3'untranslated ends of bTP-1 and oTP-1 cDNA, which is not extended to bovine IFNa,, cDNA (5). Finally, the organization of up-stream promoter ...
Biology and computers
Biology and computers

... contribute more to the final structure than others. (This has been demonstrated by systematic mutation experiments in which each consecutive position in a sequence is substituted by a spectrum of amino acids. Some positions are remarkably tolerant of substitution, while others have unique requiremen ...
Chapter 5: Structure and Function of Macromolecules
Chapter 5: Structure and Function of Macromolecules

...  A polypeptide is a polymer of amino acids connected in a specific sequence (pp. 71-74, FIGURES 5.15-5.16, TABLE 5.1) Polypeptides are constructed from 20 different amino acids, each with a characteristic side chain (R group). The carboxyl and amino groups of adjacent amino acids link together in p ...
ADP-ribosyltransferases: plastic tools for inactivating protein and
ADP-ribosyltransferases: plastic tools for inactivating protein and

... and has hence been confirmed in all other ADPRTs thus examined (Bazan and Koch-Nolte, 1997; Domenighini and Rappuoli, 1996). Two other amino acid residues stabilise the active site and are also important for catalytic activity. These are histidine and tyrosine (H in b1, Y in b2) in diphtheria toxin ...
How is DNA*s Genetic Code Used to Make Proteins?
How is DNA*s Genetic Code Used to Make Proteins?

Lecture Notes - Math
Lecture Notes - Math

Protein Conformation and Function
Protein Conformation and Function

... provide variety and determine the chemical and physical properties. ...
Article Reference - Archive ouverte UNIGE
Article Reference - Archive ouverte UNIGE

... Stemming: the process of reducing a word to its simplest form for efficient searching by removing prefixes and suffixes (endings), such as (in English) -s, -ed, and -ing. Stop words: A word which is ignored in a query because it is so common that it does not contribute to the search; typical stop wo ...
DNA sequencing: methods
DNA sequencing: methods

... From the BLAST tutorial: Although hits with E values much higher than 0.1 are unlikely to reflect true sequence relatives, it is useful to examine hits with lower significance (E values between 0.1 and 10) for short regions of similarity. In the absence of longer similarities, these short regions ma ...
What Are The Best Supplements For Muscle Building
What Are The Best Supplements For Muscle Building

... Creatine Monohydrate hit the stage about 15 years ago, and since then has revolutionized the supplements world. Creatine is the best selling supplement out there. Creatine is a compound made of three amino acids: Arginine, Glycine and Methionine. Although Creatine can be found in food like steak and ...
Chapter 17. - RMC Science Home
Chapter 17. - RMC Science Home

... Deletion = lose one or more bases ...
Globin Gene Exercise
Globin Gene Exercise

... Why are eukaryotic genes split like this? Why would higher animals adopt this complicated mechanism to encode the information for making proteins? (Some genes are known to have hundreds of introns.) Are all introns in all proteins spliced out by the same spliceosome structure or does each gene have ...
week9_DNA&geneExpression.bak
week9_DNA&geneExpression.bak

... 2. Example of Transcriptional Control: The Lac Operon in Bacteria • Operon: group of nucleotide sequences including an operator, a promoter, and one or more genes that are controlled as a unit to produce messenger RNA (mRNA) *The Operon model is one example of gene expression regulation ...
Nucleic Acids and Protein Synthesis
Nucleic Acids and Protein Synthesis

... strand is translated into a sequence of amino acids to make a protein B. A series of three nucleotides on RNA, or codon, codes for one amino acid. C. There are 64 codons and only 20 amino acids, so several codons can code for the same amino acid D. There are also a start codon (AUG) and ...
Mistakes Happen
Mistakes Happen

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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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