Download Curriculum Vitae

Curriculum Vitae
Personal information:
-Name: Ye Xie
-Gender: Male
-Nationality: P.R. China
-Date of Birth: 1988-02-07
-Address: 300 George Street, Suite 8100, New Haven, CT, 06511
-Email: [email protected]; [email protected]
Academic Education:
2016Postdoctoral Associate in Department of Ophthalmology and Visual Science,
Yale Medical School
2010-2016 Ph.D. in Botany, specialization in molecular biology, SIPPE, SIBS, CAS,
Shanghai, China.
2006-2010 B.S. in Biotechnology, National training base for life science, Nanjing
Agricultural Universtiy, Nanjing, China.
Interests:
biomedical research, plant metabolism, molecular biology, and intracellular signal
transduction
Research/working Experience:
Using anthocyanin as a well-known biomarker, my previous work mainly focuses on the
molecular mechanisms of hormone signal transduction and transcriptional regulatory
complexes by adapting diverse molecular, biochemical, cellular and genetic techniques.
Anthocyanins, a class of plant secondary metabolites distributed ubiquitously in the
plant kingdom, endow plants with a colorful world and contribute plants to fight against
environemtental stresses, as well as providing human-health properties. I found that
DELLA proteins, key repressors of Gibberellin (GA) signaling, mediate the
GA-suppressed anthocyanin biosynthesis. The GA-suppressed anthocyanin biosynthesis
is dependent on MBW transcriptional complex, which is comprosed of the MYB, bHLH
and WD40 proteins in higher plants. My results demonstrated that under diverse stress
conditions, DELLA proteins accumulate and directly bind to MYBL2 and JAZ proteins, two
repressors of the MBW complex, and then release MYB and bHLH subunits to form the
active MBW complex, which finally activates anthocyanin biosynthetic pathway. This work
was selected and I was invited to give oral speeches in the 26th International Conference
on Arabidopsis Research. This work has recently published in Molecualr Plant.
Otherwise, by adopting forward genetic method and RNA-seq analysis, I identified several
genes might be involved in anthocyanin biodegradation.
Now, I am studying the in vivo reprogramming of Muller glial cells in mouse retina.
Publications:
Xie, Y., Tan, H., Ma, Z., and Huang, J. (2016). DELLA proteins promote anthocyanin
biosynthesis via sequestering MYBL2 and JAZ suppressors of the MYB/bHLH/WD40
complex in Arabidopsis thaliana. Mol. Plant. 9: 711-721.
Xie, Y., Sun, Y., and Huang, J. (2013). Anthocyanin modification in Arabidopsis. Plant
Physiology Journal. 49, 101-110 (In Chinese)
Xie, Y., Sun, Y., Li, D., and Huang, J. (2013). MicroRNA828 negatively regulates
sucrose-induced anthocyanin biosynthesis in Arabidopsis. Plant Physiology Journal. 49,
188-194 (In Chinese)
Hu, F., Zhu, Y., Wu, W., Xie, Y., and Huang, J. (2015). Leaf variegation of Thylakoid
Formation1 Is suppressed by mutations of specific σ-factors in Arabidopsis. Plant Physiol.
168: 1066-1075.
Gan, Y., Li, H., Xie, Y., Wu, W., Li, M., Wang, X., and Huang, J. (2014). THF1 mutations
lead to increased basal and wound-induced levels of oxylipins that stimulate anthocyanin
biosynthesis via COI1 signaling in Arabidopsis. J. Integr. Plant Biol. 56: 916-927.
Chen, Q., Man, C., Li, D, Tan, H., Xie, Y., and Huang J. Anthocyanin Biosynthesis is
regulated by differential arogenate dehydratase isoforms in Arabidopsis thaliana. In
revision.
Honors and Awards:
2012-2013, Excellent Student Award from Unversity of Chinese Academy of Sciences.
2014, Best speech award at 8th Youth Conference on Plant Biology of the Shanghai
Society for Plant Biology.
Attended Conferences and Presentations:
The 3rd International Conference on Plant Metabolism. Xiamen, China, 2014.
Title: The molecular mechanism of gibberellin-regulated anthocyanin biosynthesis.
The 26th International Conference on Arabidopsis Research. Paris, France. July 2015.
Title: DELLA proteins promote anthocyanin biosynthesis via sequestering MYBL2
and JAZ supressors of the MYB/bHLH/WD40 complex in Arabidopsis thaliana.
Academic exchange in Friedrich Miescher Institute for Biomedical Research (FMI). Basel,
Switzerland, April 2016.
Technique skills:
Molecular Biology
Vector construction and DNA cloning; Aritificial microRNA-mediated gene silencing; DNA
and RNA extraction; PCR, RT-PCR, and Quantitative Real-Time PCR; TAIL-PCR and
map-based cloning; Yeast two-hybrid assay; Promoter activity analysis by LUC/REN ratio;
Chromatin immunoprecipitation (ChIP)
Biochemistry
Heterologous protein expression in E. coli, protein purification and characterization;
GST/His pull-down and in vitro protein competitively binding assay; Protein extraction;
Co-immunoprecipitation (Co-IP); Protein electrophoresis (SDS-PAGE) and Western blot
Cell Biology
Confocal microscopy for subcellular localization of FP-tagged proteins; bimolecular
fluorescence complementation (BiFC) in cells; Histological analysis of GUS enzyme
acitivity in Arabidopsis tissues; Arabidopsis protoplast preparation and transfection;
Phyto-chemical analysis and plant culture
High Performance Liquid Chromatography (HPLC) analysis; Arabidopsis and Nicotiana
benthamiana tissue culture under normal or stess conditions; Arabidopsis crossing,
phenotyping, hormonal and chemical treatment;
Bioinformatics
Basic Blast; Proficient use of software (ex. BioEdit); Experienced in using online tools
(The MEME Suite for motif-based sequence analysis tools; ATTED-II for estimate gene
functions via co-regulated gene relationship; GENEVESTIGATOR for gene expression)