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Curriculum Vitae Personal information: -Name: Ye Xie -Gender: Male -Nationality: P.R. China -Date of Birth: 1988-02-07 -Address: 300 George Street, Suite 8100, New Haven, CT, 06511 -Email: [email protected]; [email protected] Academic Education: 2016Postdoctoral Associate in Department of Ophthalmology and Visual Science, Yale Medical School 2010-2016 Ph.D. in Botany, specialization in molecular biology, SIPPE, SIBS, CAS, Shanghai, China. 2006-2010 B.S. in Biotechnology, National training base for life science, Nanjing Agricultural Universtiy, Nanjing, China. Interests: biomedical research, plant metabolism, molecular biology, and intracellular signal transduction Research/working Experience: Using anthocyanin as a well-known biomarker, my previous work mainly focuses on the molecular mechanisms of hormone signal transduction and transcriptional regulatory complexes by adapting diverse molecular, biochemical, cellular and genetic techniques. Anthocyanins, a class of plant secondary metabolites distributed ubiquitously in the plant kingdom, endow plants with a colorful world and contribute plants to fight against environemtental stresses, as well as providing human-health properties. I found that DELLA proteins, key repressors of Gibberellin (GA) signaling, mediate the GA-suppressed anthocyanin biosynthesis. The GA-suppressed anthocyanin biosynthesis is dependent on MBW transcriptional complex, which is comprosed of the MYB, bHLH and WD40 proteins in higher plants. My results demonstrated that under diverse stress conditions, DELLA proteins accumulate and directly bind to MYBL2 and JAZ proteins, two repressors of the MBW complex, and then release MYB and bHLH subunits to form the active MBW complex, which finally activates anthocyanin biosynthetic pathway. This work was selected and I was invited to give oral speeches in the 26th International Conference on Arabidopsis Research. This work has recently published in Molecualr Plant. Otherwise, by adopting forward genetic method and RNA-seq analysis, I identified several genes might be involved in anthocyanin biodegradation. Now, I am studying the in vivo reprogramming of Muller glial cells in mouse retina. Publications: Xie, Y., Tan, H., Ma, Z., and Huang, J. (2016). DELLA proteins promote anthocyanin biosynthesis via sequestering MYBL2 and JAZ suppressors of the MYB/bHLH/WD40 complex in Arabidopsis thaliana. Mol. Plant. 9: 711-721. Xie, Y., Sun, Y., and Huang, J. (2013). Anthocyanin modification in Arabidopsis. Plant Physiology Journal. 49, 101-110 (In Chinese) Xie, Y., Sun, Y., Li, D., and Huang, J. (2013). MicroRNA828 negatively regulates sucrose-induced anthocyanin biosynthesis in Arabidopsis. Plant Physiology Journal. 49, 188-194 (In Chinese) Hu, F., Zhu, Y., Wu, W., Xie, Y., and Huang, J. (2015). Leaf variegation of Thylakoid Formation1 Is suppressed by mutations of specific σ-factors in Arabidopsis. Plant Physiol. 168: 1066-1075. Gan, Y., Li, H., Xie, Y., Wu, W., Li, M., Wang, X., and Huang, J. (2014). THF1 mutations lead to increased basal and wound-induced levels of oxylipins that stimulate anthocyanin biosynthesis via COI1 signaling in Arabidopsis. J. Integr. Plant Biol. 56: 916-927. Chen, Q., Man, C., Li, D, Tan, H., Xie, Y., and Huang J. Anthocyanin Biosynthesis is regulated by differential arogenate dehydratase isoforms in Arabidopsis thaliana. In revision. Honors and Awards: 2012-2013, Excellent Student Award from Unversity of Chinese Academy of Sciences. 2014, Best speech award at 8th Youth Conference on Plant Biology of the Shanghai Society for Plant Biology. Attended Conferences and Presentations: The 3rd International Conference on Plant Metabolism. Xiamen, China, 2014. Title: The molecular mechanism of gibberellin-regulated anthocyanin biosynthesis. The 26th International Conference on Arabidopsis Research. Paris, France. July 2015. Title: DELLA proteins promote anthocyanin biosynthesis via sequestering MYBL2 and JAZ supressors of the MYB/bHLH/WD40 complex in Arabidopsis thaliana. Academic exchange in Friedrich Miescher Institute for Biomedical Research (FMI). Basel, Switzerland, April 2016. Technique skills: Molecular Biology Vector construction and DNA cloning; Aritificial microRNA-mediated gene silencing; DNA and RNA extraction; PCR, RT-PCR, and Quantitative Real-Time PCR; TAIL-PCR and map-based cloning; Yeast two-hybrid assay; Promoter activity analysis by LUC/REN ratio; Chromatin immunoprecipitation (ChIP) Biochemistry Heterologous protein expression in E. coli, protein purification and characterization; GST/His pull-down and in vitro protein competitively binding assay; Protein extraction; Co-immunoprecipitation (Co-IP); Protein electrophoresis (SDS-PAGE) and Western blot Cell Biology Confocal microscopy for subcellular localization of FP-tagged proteins; bimolecular fluorescence complementation (BiFC) in cells; Histological analysis of GUS enzyme acitivity in Arabidopsis tissues; Arabidopsis protoplast preparation and transfection; Phyto-chemical analysis and plant culture High Performance Liquid Chromatography (HPLC) analysis; Arabidopsis and Nicotiana benthamiana tissue culture under normal or stess conditions; Arabidopsis crossing, phenotyping, hormonal and chemical treatment; Bioinformatics Basic Blast; Proficient use of software (ex. BioEdit); Experienced in using online tools (The MEME Suite for motif-based sequence analysis tools; ATTED-II for estimate gene functions via co-regulated gene relationship; GENEVESTIGATOR for gene expression)