Biogenesis of hepatitis C virus envelope glycoproteins
... first demonstrated for E2 by Cocquerel et al. (1998). Indeed, E2 expressed in the absence of E1 can fold properly and is retained in the ER, as shown by the lack of complex glycans, its intracellular distribution and the absence of its expression at the cell surface. By using chimeric proteins const ...
... first demonstrated for E2 by Cocquerel et al. (1998). Indeed, E2 expressed in the absence of E1 can fold properly and is retained in the ER, as shown by the lack of complex glycans, its intracellular distribution and the absence of its expression at the cell surface. By using chimeric proteins const ...
Assembly of the Light-Harvesting Chlorophyll Antenna in the Green
... sequencing (Merchant et al., 2007). All wild-type controls contained about 2.5 and 1.7 fmol Chl per cell when grown under low light and medium light, respectively, and had a Chl a/b ratio ranging between 2.7 and 3.0. A lower Chl/cell under medium-light growth conditions is a compensatory response of ...
... sequencing (Merchant et al., 2007). All wild-type controls contained about 2.5 and 1.7 fmol Chl per cell when grown under low light and medium light, respectively, and had a Chl a/b ratio ranging between 2.7 and 3.0. A lower Chl/cell under medium-light growth conditions is a compensatory response of ...
Lysophospholipids and fat digestibility
... Since the 1990s the use of lysolecithins in animal nutrition has gained support among the scientific community. Although many experts agree on the benefits of its use, the mechanism by which these improve animal growth performance are often poorly understood. ...
... Since the 1990s the use of lysolecithins in animal nutrition has gained support among the scientific community. Although many experts agree on the benefits of its use, the mechanism by which these improve animal growth performance are often poorly understood. ...
Measurements of protein sequence
... structure contacts between residues distantly separated along the backbone. We found that local interactions along the amino acid chain are far more important than non-local contacts and that correlations between proximate amino acids are essentially uninformative. This suggests that knowledgebased ...
... structure contacts between residues distantly separated along the backbone. We found that local interactions along the amino acid chain are far more important than non-local contacts and that correlations between proximate amino acids are essentially uninformative. This suggests that knowledgebased ...
Assembly of AO and DHAS - Journal of Cell Science
... The data show that the matrix fraction contained virtually all our data is that the dimeric form of DHAS is the major if not the AO and DHAS from the pellet (Fig. 1C) and no the only species that crosses the peroxisomal membrane. peroxisomal membrane (Fig. 1D). In summary, there is little Having sho ...
... The data show that the matrix fraction contained virtually all our data is that the dimeric form of DHAS is the major if not the AO and DHAS from the pellet (Fig. 1C) and no the only species that crosses the peroxisomal membrane. peroxisomal membrane (Fig. 1D). In summary, there is little Having sho ...
How can biochemical reactions within cells differ from those in test
... Influence of background interactions upon reaction equilibria and rates Fig. 3 illustrates how nonspecific interactions between reactants and the background can influence the rate and/or equilibrium of a particular reaction – for example the association of two globular proteins, A and B, to form a h ...
... Influence of background interactions upon reaction equilibria and rates Fig. 3 illustrates how nonspecific interactions between reactants and the background can influence the rate and/or equilibrium of a particular reaction – for example the association of two globular proteins, A and B, to form a h ...
Observations of green fluorescent protein as a fusion partner in
... GFP as a fusion partner. In Fig. 3B, the fluorescence intensity of GFPuv increased with culture time after IPTG induction in both strains. Strain BL21 had higher initial fluorescence intensity, implying that the expression was not tightly repressed even though the parent pTrcHis plasmid contains the ...
... GFP as a fusion partner. In Fig. 3B, the fluorescence intensity of GFPuv increased with culture time after IPTG induction in both strains. Strain BL21 had higher initial fluorescence intensity, implying that the expression was not tightly repressed even though the parent pTrcHis plasmid contains the ...
(mmg) operon of Bacillus
... various industries. Hence this organism is extensively studied. The genome of this bacterium has around 4000 protein coding sequences, which include 87% of the genome sequence. Because of its ability to use different carbohydrates, the glycolytic pathway along with the TCA cycle is utilized in this ...
... various industries. Hence this organism is extensively studied. The genome of this bacterium has around 4000 protein coding sequences, which include 87% of the genome sequence. Because of its ability to use different carbohydrates, the glycolytic pathway along with the TCA cycle is utilized in this ...
Theranostics Evolution- and Structure
... dominant inherited disease, namely maturity-onset diabetes of the young, type 2 (MODY 2). MODY 2 is characterised by early onset: it usually appears before 25 years of age and presents as a mild form of hyperglycaemia. In recent years, the number of known GCK mutations has markedly increased. As a r ...
... dominant inherited disease, namely maturity-onset diabetes of the young, type 2 (MODY 2). MODY 2 is characterised by early onset: it usually appears before 25 years of age and presents as a mild form of hyperglycaemia. In recent years, the number of known GCK mutations has markedly increased. As a r ...
BICH/GENE 431 KNOWLEDGE OBJECTIVES Chapter 13 – RNA
... BICH/GENE 431 KNOWLEDGE OBJECTIVES Chapter 13 – RNA Splicing Basic terminology: exon, intron, 5’ splice site, 3’ splice site Ave # of introns per gene increases from single-cell eukaryotes up through mammals In humans, ave. size of exon is 150 nt and ave. size of intron is 3000 nt Discovery of intro ...
... BICH/GENE 431 KNOWLEDGE OBJECTIVES Chapter 13 – RNA Splicing Basic terminology: exon, intron, 5’ splice site, 3’ splice site Ave # of introns per gene increases from single-cell eukaryotes up through mammals In humans, ave. size of exon is 150 nt and ave. size of intron is 3000 nt Discovery of intro ...
Supplemental Materials
... ___________, but are also _____________________(protected from or susceptible to) malaria. Jameson is _________________ for the hemoglobin normal allele, so he has sickle cell ________ ___________, but he is _______________________(protected from or susceptible to) malaria. Their other child, Rayna, ...
... ___________, but are also _____________________(protected from or susceptible to) malaria. Jameson is _________________ for the hemoglobin normal allele, so he has sickle cell ________ ___________, but he is _______________________(protected from or susceptible to) malaria. Their other child, Rayna, ...
Changes in Typical Organelles in Developing Cotyledons of Soybean
... Figure 7. T D~ micrographs of irnrna tur e protein bodies. A) Note the difference in protein bodies between right and left cel l s. The ones in right cell have many electron translucent parts while the ones in l eft have scattered protein deposits. B) Protein bodies are not also homogeneous. Note lo ...
... Figure 7. T D~ micrographs of irnrna tur e protein bodies. A) Note the difference in protein bodies between right and left cel l s. The ones in right cell have many electron translucent parts while the ones in l eft have scattered protein deposits. B) Protein bodies are not also homogeneous. Note lo ...
From transporter to transceptor
... The Gap1 general amino acid permease in the yeast Saccharomyces cerevisiae has been studied as a model system for substrate-regulated intracellular trafficking. Research on this protein has revealed a complex set of regulatory mechanisms, not only controlling amino acid-induced endocytic internaliza ...
... The Gap1 general amino acid permease in the yeast Saccharomyces cerevisiae has been studied as a model system for substrate-regulated intracellular trafficking. Research on this protein has revealed a complex set of regulatory mechanisms, not only controlling amino acid-induced endocytic internaliza ...
The evolution, function, structure, and expression
... oligomeric form with a β-sandwich that forms a hollow ball. This conservation occurs despite significant divergence in primary sequences. It is well established that sHSPs are molecular chaperones that prevent misfolding and irreversible aggregation of their client proteins. Most notably, the sHSPs ...
... oligomeric form with a β-sandwich that forms a hollow ball. This conservation occurs despite significant divergence in primary sequences. It is well established that sHSPs are molecular chaperones that prevent misfolding and irreversible aggregation of their client proteins. Most notably, the sHSPs ...
Scaffold nucleoporins Nup188 and Nup192 share
... To determine the structure of Nup188 we first expressed full-length protein from Saccharomyces cerevisiae (Sc) and the thermophilic fungus Myceliophthora thermophila (Mt) (Berka et al., 2011). Although the Mt homolog behaved better in vitro, it did not yield crystals of the full-length protein. Usin ...
... To determine the structure of Nup188 we first expressed full-length protein from Saccharomyces cerevisiae (Sc) and the thermophilic fungus Myceliophthora thermophila (Mt) (Berka et al., 2011). Although the Mt homolog behaved better in vitro, it did not yield crystals of the full-length protein. Usin ...
Determination of Protein Concentrations Using AAA
... background peaks that compromise trace-level analysis. Without hydrolysis, the amino acids are not apparent. To qualify water for use in sample dilutions, hydrolyze an aliquot as you would a protein, and measure peaks having retention times of amino acids. Filters are often manufactured using glycer ...
... background peaks that compromise trace-level analysis. Without hydrolysis, the amino acids are not apparent. To qualify water for use in sample dilutions, hydrolyze an aliquot as you would a protein, and measure peaks having retention times of amino acids. Filters are often manufactured using glycer ...
Pore-Forming Proteins and Adaptation of Living Organisms to
... allel to the membrane, and then C-terminal helices of 12 peptides insert into the membrane and form a pore [4]. The second type of pore-forming cytolysins comprises antimicrobial melittin-like peptides that display a higher hemolytic activity as compared with cecropin-like peptides. They are used by ...
... allel to the membrane, and then C-terminal helices of 12 peptides insert into the membrane and form a pore [4]. The second type of pore-forming cytolysins comprises antimicrobial melittin-like peptides that display a higher hemolytic activity as compared with cecropin-like peptides. They are used by ...
Mitochondria, Chloroplasts, Peroxisomes - Beck-Shop
... Similar to cells, mitochondria divide, but unlike most cells, they also fuse with other mitochondria. These fusion and division reactions were first observed nearly one hundred years ago. Now it is appreciated that a balance between ongoing fusion and division determines the number of mitochondria w ...
... Similar to cells, mitochondria divide, but unlike most cells, they also fuse with other mitochondria. These fusion and division reactions were first observed nearly one hundred years ago. Now it is appreciated that a balance between ongoing fusion and division determines the number of mitochondria w ...
GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 Are
... microscope, we isolated two recessive mutants that had disorganized ER morphology (Figures 1C to 1F) and designated them ermo1 and ermo2. Both ermo1 and ermo2 developed a number of strongly GFP-labeled spherical structures that were ;1 mm in diameter (Figures 1C to 1F; see Supplemental Figures 2 and ...
... microscope, we isolated two recessive mutants that had disorganized ER morphology (Figures 1C to 1F) and designated them ermo1 and ermo2. Both ermo1 and ermo2 developed a number of strongly GFP-labeled spherical structures that were ;1 mm in diameter (Figures 1C to 1F; see Supplemental Figures 2 and ...
The unfolded protein response: an intracellular
... The unfolded protein response (UPR) is induced following the accumulation of unfolded proteins in the lumen of the endoplasmic reticulum (ER) and results in the upregulation of genes encoding ER-resident enzymes involved in protein folding1–3. Thus, the cell is able to increase the folding capacity ...
... The unfolded protein response (UPR) is induced following the accumulation of unfolded proteins in the lumen of the endoplasmic reticulum (ER) and results in the upregulation of genes encoding ER-resident enzymes involved in protein folding1–3. Thus, the cell is able to increase the folding capacity ...
CS273_SequenceSimilarity1
... F(x[1:M], y[1:N]) = F(x[1:i], y[1:j]) + F(x[i+1:M], y[j+1:N]) Key property: optimal solution to the entire problem is composed of optimal solutions to subproblems ...
... F(x[1:M], y[1:N]) = F(x[1:i], y[1:j]) + F(x[i+1:M], y[j+1:N]) Key property: optimal solution to the entire problem is composed of optimal solutions to subproblems ...
attachment 2 - Food Standards Australia New Zealand
... Expression analyses of the three proteins showed that all three were detected in the plant parts tested. The 2m EPSPS protein was lowest in the seed (approximately 22 µg/g dry weight) and highest in V10–12 leaves (approximately 2323 µg/g dry weight). AAD-12 was lowest in the roots (approximately 25 ...
... Expression analyses of the three proteins showed that all three were detected in the plant parts tested. The 2m EPSPS protein was lowest in the seed (approximately 22 µg/g dry weight) and highest in V10–12 leaves (approximately 2323 µg/g dry weight). AAD-12 was lowest in the roots (approximately 25 ...
Yeast - iGEM 2007
... The yeast Saccharomyces cerevisiae (also called “baker’s yeast”) is probably the ideal eukaryotic microorganism for biological studies. ...
... The yeast Saccharomyces cerevisiae (also called “baker’s yeast”) is probably the ideal eukaryotic microorganism for biological studies. ...
Profibrillin conversion by proprotein convertases
... Mini-profibrillin and short-fibrillin constructs and sitedirected mutagenesis Four DNA segments representing the N terminus (exon 1), exons 2333, exons 30-44 and the C terminus (exons 64-65) of profibrillin-1 were amplified by RT-PCR using control fibroblast RNA and primers tailed with unique restri ...
... Mini-profibrillin and short-fibrillin constructs and sitedirected mutagenesis Four DNA segments representing the N terminus (exon 1), exons 2333, exons 30-44 and the C terminus (exons 64-65) of profibrillin-1 were amplified by RT-PCR using control fibroblast RNA and primers tailed with unique restri ...