... 30°C, with continuous shaking. Strain Cap 67 was used for electron microscopy. Strains 2E-TU and 2E-TUC were used for laccase assays. Strain H99 was used for all other experiments. Proteomic analyses were performed using strains Cap 67, H99, and 2E-TUC. Vesicle purification. Isolation of extracellul ...
The UDPase activity of the Kluyveromyces lactis Golgi GDPase has
... restored on transformation of the deletion strain with a plasmid carrying either the K. lactis (not shown) or the S. cerevisiae gene (Table I). Golgi membrane extracts from K. lactis wild type showed preferential hydrolytic activity towards GDP compared to UDP in the presence of either Ca2+ or Mn2+ ...
... restored on transformation of the deletion strain with a plasmid carrying either the K. lactis (not shown) or the S. cerevisiae gene (Table I). Golgi membrane extracts from K. lactis wild type showed preferential hydrolytic activity towards GDP compared to UDP in the presence of either Ca2+ or Mn2+ ...
Noncovalently Associated Complexes: Apomyoglobin and
... was analyzed using the Agilent HPLC-Chip MS system interfaced to an Agilent 6210 TOF MS. Myoglobin is the resident oxygen-carrying molecule of cardiac and skeletal muscle. The myoglobin complex consists of the apomyoglobin protein and Heme b. The capacity of myoglobin to bind oxygen depends on the p ...
... was analyzed using the Agilent HPLC-Chip MS system interfaced to an Agilent 6210 TOF MS. Myoglobin is the resident oxygen-carrying molecule of cardiac and skeletal muscle. The myoglobin complex consists of the apomyoglobin protein and Heme b. The capacity of myoglobin to bind oxygen depends on the p ...
X-ray Crystallographic Structure of Ibuprofen Bound to Human
... The fatty acid-binding proteins (FABPs) are a family of nine structurally related proteins, which bind to long-chain fatty acids with high affinity. Fatty acid binding proteins were first discovered in 1972, while conducting studies on the factors that regulate the uptake of intestinal fatty acids i ...
... The fatty acid-binding proteins (FABPs) are a family of nine structurally related proteins, which bind to long-chain fatty acids with high affinity. Fatty acid binding proteins were first discovered in 1972, while conducting studies on the factors that regulate the uptake of intestinal fatty acids i ...
Cardiac O-GlcNAc signaling is increased in hypertrophy and heart
... Rat models and echocardiographic/hemodynamic evaluation. Experiments on rats were approved by the Norwegian National Animal Research Committee and conformed to the Guide for the Care and Use of Laboratory Animals [National Institutes of Health (NIH) publication no. 85-23, revised 1996, US]. Ligation ...
... Rat models and echocardiographic/hemodynamic evaluation. Experiments on rats were approved by the Norwegian National Animal Research Committee and conformed to the Guide for the Care and Use of Laboratory Animals [National Institutes of Health (NIH) publication no. 85-23, revised 1996, US]. Ligation ...
A 29 kDa Intracellular Chloride Channel p64H1 Is Associated with
... methods were approved by the Weill Medical College of Cornell University Institutional C are and Use Committee. Six Sprague Dawley rats (Taconic, Germantown, N Y) were used in the light microscopy immunocytochemical experiments. All rats were anesthetized with Nembutal (150 mg / kg, i.p.) and sequen ...
... methods were approved by the Weill Medical College of Cornell University Institutional C are and Use Committee. Six Sprague Dawley rats (Taconic, Germantown, N Y) were used in the light microscopy immunocytochemical experiments. All rats were anesthetized with Nembutal (150 mg / kg, i.p.) and sequen ...
Biochemistry, proteomics, and phosphoproteomics of plant
... For this reason and space considerations, this discussion of proteomics and post-translational modifications will focus mainly on Arabidopsis suspension cells and etiolated rice seedlings. Bioinformatic analysis of the Arabidopsis genome, using primarily prediction algorithms, has estimated that mit ...
... For this reason and space considerations, this discussion of proteomics and post-translational modifications will focus mainly on Arabidopsis suspension cells and etiolated rice seedlings. Bioinformatic analysis of the Arabidopsis genome, using primarily prediction algorithms, has estimated that mit ...
Structural biology and drug discovery
... Identifying ligand binding regions As structural genomics projects produce more 3D structures, with many of the proteins recognized only from their gene sequences, it is becoming increasingly important to develop computational methods that will identify sites involved in productive intermolecular in ...
... Identifying ligand binding regions As structural genomics projects produce more 3D structures, with many of the proteins recognized only from their gene sequences, it is becoming increasingly important to develop computational methods that will identify sites involved in productive intermolecular in ...
Osmoadaptative Strategy and Its Molecular
... kirbyi strain AS12B (Park and Simpson 2011). Both are obligate halophiles that cannot grow at salinities <7.5% under laboratory conditions. The AS12B strain of P. kirbyi grows optimally at a slightly lower salt concentration than Haloc. seosinensis EHF34 (12% vs. 15%, respectively). Concordantly, Ha ...
... kirbyi strain AS12B (Park and Simpson 2011). Both are obligate halophiles that cannot grow at salinities <7.5% under laboratory conditions. The AS12B strain of P. kirbyi grows optimally at a slightly lower salt concentration than Haloc. seosinensis EHF34 (12% vs. 15%, respectively). Concordantly, Ha ...
A Study of the Membrane–Water Interface Region of Membrane
... acid composition. The interface and transmembrane helices consist mainly of hydrophobic and polar aromatic (Trp, Tyr) residues; the latter are particularly abundant in the interface helices. Gly and Pro are enriched in the irregular structure, together with other polar residues such as Asn and Ser. ...
... acid composition. The interface and transmembrane helices consist mainly of hydrophobic and polar aromatic (Trp, Tyr) residues; the latter are particularly abundant in the interface helices. Gly and Pro are enriched in the irregular structure, together with other polar residues such as Asn and Ser. ...
Structural and functional study of K453E mutant protective
... According to the crystal structure reported by Rudenko et al. (1995), human PPCA consists of “core” and “cap” domains. The core domain contains ten central β-sheets, and an additional ten α-helices and two small β-sheets occur on both sides of the central β-sheets. The cap domain is divided into a “ ...
... According to the crystal structure reported by Rudenko et al. (1995), human PPCA consists of “core” and “cap” domains. The core domain contains ten central β-sheets, and an additional ten α-helices and two small β-sheets occur on both sides of the central β-sheets. The cap domain is divided into a “ ...
`slow-twitch type` mitochondria, but does not
... compare protein expression patterns between PV– ⁄ – and WT TA. No significant differences in protein profiles were observed, with the exception of the missing spot corresponding to PV in PV– ⁄ – samples (Fig. 2). Several proteins were identified by comparison of 2D gels with a 2D gel of mouse gastrocne ...
... compare protein expression patterns between PV– ⁄ – and WT TA. No significant differences in protein profiles were observed, with the exception of the missing spot corresponding to PV in PV– ⁄ – samples (Fig. 2). Several proteins were identified by comparison of 2D gels with a 2D gel of mouse gastrocne ...
Answers - Study of Life
... D. nucleoid E. nucleolus Suppose radioactive thymine was added to an actively dividing culture of E.coli bacteria. What would happen if a cell replicated once in the presence of this radioactive base? A. one of the daughter cells, but not the other, would have radioactive DNA B. neither of the two d ...
... D. nucleoid E. nucleolus Suppose radioactive thymine was added to an actively dividing culture of E.coli bacteria. What would happen if a cell replicated once in the presence of this radioactive base? A. one of the daughter cells, but not the other, would have radioactive DNA B. neither of the two d ...
Biological electron-transfer reactions
... (GRTln K) if the reduction potentials of the reactants are known. Since the pioneering studies of J.B. Conant concerning the oxidation–reduction equilibrium of haemoglobin in the 1920s, several methods have been used to study the electrochemical properties of proteins that possess metals, flavin ...
... (GRTln K) if the reduction potentials of the reactants are known. Since the pioneering studies of J.B. Conant concerning the oxidation–reduction equilibrium of haemoglobin in the 1920s, several methods have been used to study the electrochemical properties of proteins that possess metals, flavin ...
Protein for Athletes
... 1 repetition maximum. After 10 weeks, no differences between body composition or strength were seen between groups, suggesting that those individuals who are resistance trained will gain no further benefits from ingesting additional protein either before or after exercise. ...
... 1 repetition maximum. After 10 weeks, no differences between body composition or strength were seen between groups, suggesting that those individuals who are resistance trained will gain no further benefits from ingesting additional protein either before or after exercise. ...
PDF
... information on the sub-cellular localization of aaRSs in cyanobacterial cells is limited and a global picture of the distribution of the full complement of aaRSs is lacking. This information is fundamental to understand how the initial steps of translation occur in these organisms and whether they a ...
... information on the sub-cellular localization of aaRSs in cyanobacterial cells is limited and a global picture of the distribution of the full complement of aaRSs is lacking. This information is fundamental to understand how the initial steps of translation occur in these organisms and whether they a ...
Endomembrane proton pumps: connecting membrane and vesicle
... V-ATPases constitute a family of highly conserved, multisubunit proton-pumps that are found throughout the endomembrane systems of all eukaryotes [3]. They share a common ancestor with F-ATPases and, like their distant relatives, are composed of two subcomplexes. The peripheral V1 complex, which con ...
... V-ATPases constitute a family of highly conserved, multisubunit proton-pumps that are found throughout the endomembrane systems of all eukaryotes [3]. They share a common ancestor with F-ATPases and, like their distant relatives, are composed of two subcomplexes. The peripheral V1 complex, which con ...
INFERRING PROPERTY SELECTION PRESSURE FROM
... striking absence of cysteine raises a red flag. This “hole” causes us to doubt whether this property is actually being selected for. Thus, we make an additional assumption: when selection is based on a single property, fitness is unimodal (i.e. holes constitute negative evidence). This new assumption ...
... striking absence of cysteine raises a red flag. This “hole” causes us to doubt whether this property is actually being selected for. Thus, we make an additional assumption: when selection is based on a single property, fitness is unimodal (i.e. holes constitute negative evidence). This new assumption ...
Scavenging of 14-3-3 proteins reveals their involvement in the cell
... of endogenous 14-3-3 proteins using an artificial 14-3-3scavenger protein. This was constructed (Fig. 1A) by fusion of protein G, the tetramer-forming coiled-coil domain pLI (Harbury et al., 1993) and the R18 peptide that binds 14-3-3 proteins with high affinity (Wang et al., 1999). We refer to this ...
... of endogenous 14-3-3 proteins using an artificial 14-3-3scavenger protein. This was constructed (Fig. 1A) by fusion of protein G, the tetramer-forming coiled-coil domain pLI (Harbury et al., 1993) and the R18 peptide that binds 14-3-3 proteins with high affinity (Wang et al., 1999). We refer to this ...
Using intrinsically fluorescent proteins for plant cell
... both of the transgenes can, in theory, occur if the same promoters are used (Baulcombe, 2005). In practice, the first and second problems are often sorted out during the production and selection of stably transformed plants or cell lines because only dimly expressing cells are recovered (which may b ...
... both of the transgenes can, in theory, occur if the same promoters are used (Baulcombe, 2005). In practice, the first and second problems are often sorted out during the production and selection of stably transformed plants or cell lines because only dimly expressing cells are recovered (which may b ...
Two-step and one-step secretion mechanisms in Gram
... Every pilus subunit possesses a 10–20 residue long disordered Nte (N-terminal extension), which, similar to the G1 β-strand of the chaperone, contains alternating hydrophobic residues. This Nte was shown not to be part of the Ig-like fold [36]. During the course of polymerization, the Nte of the inc ...
... Every pilus subunit possesses a 10–20 residue long disordered Nte (N-terminal extension), which, similar to the G1 β-strand of the chaperone, contains alternating hydrophobic residues. This Nte was shown not to be part of the Ig-like fold [36]. During the course of polymerization, the Nte of the inc ...
Powerpoint - Master Brewers Association
... The University of Nottingham, Loughborough, England. ...
... The University of Nottingham, Loughborough, England. ...
Abundant Expression of ras Proteins in Aplysia Neurons
... We used indirect immunofluorescence with antibody Y13259 to determine where ras proteins are localized within the Aplysia tissues. This antibody reacts with the products of all three known mammalian ras proto-oncogenes as well as with the products of ras genes in S. cerevisiae, Dictyostelium, and Dr ...
... We used indirect immunofluorescence with antibody Y13259 to determine where ras proteins are localized within the Aplysia tissues. This antibody reacts with the products of all three known mammalian ras proto-oncogenes as well as with the products of ras genes in S. cerevisiae, Dictyostelium, and Dr ...
