Theoretical studies of Membrane Proteins
... Membrane proteins are a large and important class of proteins. They are responsible for several of the key functions in a living cell, e.g. transport of nutrients and ions, cell-cell signaling, and cell-cell adhesion. Despite their importance it has not been possible to study their structure and org ...
... Membrane proteins are a large and important class of proteins. They are responsible for several of the key functions in a living cell, e.g. transport of nutrients and ions, cell-cell signaling, and cell-cell adhesion. Despite their importance it has not been possible to study their structure and org ...
Red blood cell glycophorins
... the genomic structure of GPE is LYCOPHORIN-A (GPA), GPB, GPC, and GPD well-characterized, search for the protein encoded by this constitute a group of red blood cell (RBC) transmemgene has yielded negative results. All the expressed glycophbrane proteins that, although perhaps not widely appreciorin ...
... the genomic structure of GPE is LYCOPHORIN-A (GPA), GPB, GPC, and GPD well-characterized, search for the protein encoded by this constitute a group of red blood cell (RBC) transmemgene has yielded negative results. All the expressed glycophbrane proteins that, although perhaps not widely appreciorin ...
Amyotrophic lateral sclerosis: Protein
... 2-DE and bioinformatics. When used within the framework of proper experimental designs, proteomic technologies can be very powerful at providing clues or answers to many crucial biological questions, including changes in protein expression, PTM, protein-protein interactions and protein activities. T ...
... 2-DE and bioinformatics. When used within the framework of proper experimental designs, proteomic technologies can be very powerful at providing clues or answers to many crucial biological questions, including changes in protein expression, PTM, protein-protein interactions and protein activities. T ...
Regulators of Lysosome Function and Dynamics in Caenorhabditis
... identified homozygous, protein-altering mutations in two genes on chromosome II, Y51H1A.2 and sre-46. RNAi of Y51H1A.2, but not of sre-46, yielded the cup-14 mutant bright coelomocyte GFP defect in Pmyo-3::ssGFP worms. Furthermore, cup-14(cd42); Pmyo-3::ssGFP did not complement an existing null alle ...
... identified homozygous, protein-altering mutations in two genes on chromosome II, Y51H1A.2 and sre-46. RNAi of Y51H1A.2, but not of sre-46, yielded the cup-14 mutant bright coelomocyte GFP defect in Pmyo-3::ssGFP worms. Furthermore, cup-14(cd42); Pmyo-3::ssGFP did not complement an existing null alle ...
Ribosome-tethered molecular chaperones
... associate specifically with ribosomes and bind to polypeptide chains that have just emerged from the tunnel. In addition, non-ribosome-bound chaperones act on longer nascent chains, either during the process of translation, or after they have been released from the ribosome. Although chaperones carr ...
... associate specifically with ribosomes and bind to polypeptide chains that have just emerged from the tunnel. In addition, non-ribosome-bound chaperones act on longer nascent chains, either during the process of translation, or after they have been released from the ribosome. Although chaperones carr ...
Green Fluorescent Protein: A Reporter Molecule
... Study of biological processes (example: synthesis of proteins) ...
... Study of biological processes (example: synthesis of proteins) ...
Comparison of sequence-based and structure
... percentage sequence identity (%I) (figure 1b). The value shown within the bracket is the standard deviation of correlation coefficient evaluated by bootstrap method. 3.2a Poor correlation with high sequence identities: It could be noted from table 2 that, while the majority of the families have low av ...
... percentage sequence identity (%I) (figure 1b). The value shown within the bracket is the standard deviation of correlation coefficient evaluated by bootstrap method. 3.2a Poor correlation with high sequence identities: It could be noted from table 2 that, while the majority of the families have low av ...
PDF - Biotechnology for Biofuels
... various anaerobic and facultative anaerobic bacteria capable of fermentative hydrogen production [4]. H2 production in living organisms is always dependent on the presence of H2-producing enzymes such as hydrogenases and nitrogenases. [FeFe] hydrogenases, which are especially abundant in clostridia, ...
... various anaerobic and facultative anaerobic bacteria capable of fermentative hydrogen production [4]. H2 production in living organisms is always dependent on the presence of H2-producing enzymes such as hydrogenases and nitrogenases. [FeFe] hydrogenases, which are especially abundant in clostridia, ...
State of the Art Manufacturing of Protein Hydrolysates
... hydrolysates with respect to the requirements of animal cells is not as well known as that seen in defined media (Pasupuleti 2007). In this chapter, we focus mainly on the manufacturing of protein hydrolysates related to biotechnology applications and it is beyond the scope of this chapter to cover ...
... hydrolysates with respect to the requirements of animal cells is not as well known as that seen in defined media (Pasupuleti 2007). In this chapter, we focus mainly on the manufacturing of protein hydrolysates related to biotechnology applications and it is beyond the scope of this chapter to cover ...
Substrate specificity of the periplasmic dipeptide
... It has been recognized for some time that the unusually broad substrate specificities of peptide transporters make them particularly attractive targets to exploit for delivery of therapeutic agents. The utility of this approach has been endorsed by the finding that a range of such compounds occur na ...
... It has been recognized for some time that the unusually broad substrate specificities of peptide transporters make them particularly attractive targets to exploit for delivery of therapeutic agents. The utility of this approach has been endorsed by the finding that a range of such compounds occur na ...
Proteomics Principles and Techniques Prof. Sanjeeva Srivastava
... absorption at 280 nanometers is used for the protein concentration determination. I will refresh some of the concepts discussed in the amino acid structures and properties in following animation. Amino acids are the building blocks or monomers that make a protein; they consist of a central alpha car ...
... absorption at 280 nanometers is used for the protein concentration determination. I will refresh some of the concepts discussed in the amino acid structures and properties in following animation. Amino acids are the building blocks or monomers that make a protein; they consist of a central alpha car ...
Tsui, S, Dai, T, Warren, ST and Yen, P: Association of the mouse infertility factor DAZL1 with actively translating polyribosomes. Biology of Reproduction 62:1655-1660 (2000).
... oligo(dT) beads demonstrates that the association is mediated through the binding of DAZL1 to poly(A) RNA. Our results suggest that DAZL1 is involved in germ-cell specific regulation of mRNA translation. ...
... oligo(dT) beads demonstrates that the association is mediated through the binding of DAZL1 to poly(A) RNA. Our results suggest that DAZL1 is involved in germ-cell specific regulation of mRNA translation. ...
Different packing of external residues can explain differences in the
... residues, it was shown that Lys has a much greater number of accessible rotamers in the folded state than Arg has. To demonstrate the stabilizing role of lysine residues, the authors replaced Arg with Lys (in silico) and analyzed the unfolding simulations. These simulations have shown that the modif ...
... residues, it was shown that Lys has a much greater number of accessible rotamers in the folded state than Arg has. To demonstrate the stabilizing role of lysine residues, the authors replaced Arg with Lys (in silico) and analyzed the unfolding simulations. These simulations have shown that the modif ...
Integrative Assignment - California State University
... Because it is so difficult to get enough absolutely pure Complex IV from human tissue to crystalize… this is formally all we can say about the structure of Cytochrome c Oxidase from human mitochondria. However, it is less challenging to obtain a large amount of beef heart mitochondria from slaughte ...
... Because it is so difficult to get enough absolutely pure Complex IV from human tissue to crystalize… this is formally all we can say about the structure of Cytochrome c Oxidase from human mitochondria. However, it is less challenging to obtain a large amount of beef heart mitochondria from slaughte ...
The stability and nuclear localization of the transcription factor RAP2
... with a subsequent reduction in ATP availability. Concomitantly, the metabolism of storage products such as starch, proteins and lipids is reduced (Geigenberger 2003). Moreover, energy-saving pathways are favoured over those that are more expensive in terms of ATP (Bologa et al. 2003). When oxygen be ...
... with a subsequent reduction in ATP availability. Concomitantly, the metabolism of storage products such as starch, proteins and lipids is reduced (Geigenberger 2003). Moreover, energy-saving pathways are favoured over those that are more expensive in terms of ATP (Bologa et al. 2003). When oxygen be ...
Descriptions of translation related genes that
... Ribonucleoprotein involved in regulating mRNA translation, transport, processing PUF protein family member, involved in DNA metabolism and transcription Part of 66S ribosomal particle, participate in ribosome assembly and maintenance Nuclear pore subunit, interact with Mex67p and involved in RNA pro ...
... Ribonucleoprotein involved in regulating mRNA translation, transport, processing PUF protein family member, involved in DNA metabolism and transcription Part of 66S ribosomal particle, participate in ribosome assembly and maintenance Nuclear pore subunit, interact with Mex67p and involved in RNA pro ...
Vps41 Phosphorylation and the Rab Ypt7 Control the Targeting of
... link the in vivo and in vitro effects observed in the yck3⌬ mutant to Vps41 function, we searched for the phosphorylation sites in the protein. The Vps41 phosphorylation results in a size shift on gels and is observed in vitro if vacuole-containing membranes are incubated in the presence of ATP (Fig ...
... link the in vivo and in vitro effects observed in the yck3⌬ mutant to Vps41 function, we searched for the phosphorylation sites in the protein. The Vps41 phosphorylation results in a size shift on gels and is observed in vitro if vacuole-containing membranes are incubated in the presence of ATP (Fig ...
Discovery of frameshifting in Alphavirus 6K resolves a 20
... occurs at a conserved UUUUUUA motif within the sequence encoding 6K, resulting in the synthesis of an additional protein, termed TF (TransFrame protein; ~8 kDa), in which the Cterminal amino acids are encoded by the -1 frame. The presence of TF in the Semliki Forest virion was confirmed by mass spec ...
... occurs at a conserved UUUUUUA motif within the sequence encoding 6K, resulting in the synthesis of an additional protein, termed TF (TransFrame protein; ~8 kDa), in which the Cterminal amino acids are encoded by the -1 frame. The presence of TF in the Semliki Forest virion was confirmed by mass spec ...
Transcriptional activity and role of plasmids of
... of function and decrease in cell permeability inhibits cell growth and can result in cell death [2 – 3]. Further, as an exchange of proton and divalent cations (Mn2+) is involved in PMF regulation, intracellular depletion of Mn2+ levels results in both oxidative stress and a loss of some enzymatic f ...
... of function and decrease in cell permeability inhibits cell growth and can result in cell death [2 – 3]. Further, as an exchange of proton and divalent cations (Mn2+) is involved in PMF regulation, intracellular depletion of Mn2+ levels results in both oxidative stress and a loss of some enzymatic f ...
Prospects for understanding avirulence gene function Frank F White
... result in expression patterns that are representative of those under the endogenous promoter. Tests using the wild-type promoter are in progress (G Martin, personal communication). Rpm1, the R-gene product corresponding to AvrRpm1, is associated with the inner plasma membrane [44]. AvrRpm1 has a myr ...
... result in expression patterns that are representative of those under the endogenous promoter. Tests using the wild-type promoter are in progress (G Martin, personal communication). Rpm1, the R-gene product corresponding to AvrRpm1, is associated with the inner plasma membrane [44]. AvrRpm1 has a myr ...
Molecular cloning, over-expression, developmental regulation and
... of various actin-binding proteins. Using conventional protein chemical methods, Ampe and Vandekerckhove (1987) determined approximately 88% of the complete amino acid sequence of fragminP. However, to obtain a clear understanding of its function (i.e. PIP2-binding, enhancement of actin phosphorylati ...
... of various actin-binding proteins. Using conventional protein chemical methods, Ampe and Vandekerckhove (1987) determined approximately 88% of the complete amino acid sequence of fragminP. However, to obtain a clear understanding of its function (i.e. PIP2-binding, enhancement of actin phosphorylati ...
ER and vacuoles: never been closer
... tonoplast intrinsic protein α-TIP, unlike the soluble vacuolar protein phytohemagglutinin (PHA), can reach the lytic vacuole even after brefeldin A (BFA) or monensin treatment when transiently expressed in tobacco leaves, suggesting the presence of different vacuolar transport routes. Few years late ...
... tonoplast intrinsic protein α-TIP, unlike the soluble vacuolar protein phytohemagglutinin (PHA), can reach the lytic vacuole even after brefeldin A (BFA) or monensin treatment when transiently expressed in tobacco leaves, suggesting the presence of different vacuolar transport routes. Few years late ...
Membrane transporters in a human genome
... The cell membrane separates a cell from the extracellular environment. While hydrophobic substances can easily cross the lipid cell membrane by simple diffusion, hydrophilic substances cannot (Guyton and Hall, 2000). There are two basic modes of cellular transport for hydrophilic substances (i.e., a ...
... The cell membrane separates a cell from the extracellular environment. While hydrophobic substances can easily cross the lipid cell membrane by simple diffusion, hydrophilic substances cannot (Guyton and Hall, 2000). There are two basic modes of cellular transport for hydrophilic substances (i.e., a ...
Identification of Surface Residues Involved in Protein
... rectly identified. With this level of success, predictions generated using this approach should be valuable for guiding experimental investigations into the roles of specific residues of a protein in its interaction with other proteins. Detailed examination of the predicted interface residues in th ...
... rectly identified. With this level of success, predictions generated using this approach should be valuable for guiding experimental investigations into the roles of specific residues of a protein in its interaction with other proteins. Detailed examination of the predicted interface residues in th ...
