NDC1 : A Nuclear Periphery Component Required for Yeast Spindle Pole Body Duplication.
... into the nuclear envelope, but instead resides on its cytoplasmic surface. Having no access to the nucleoplasm, the defective SPB cannot act as a pole of the mitotic spindle. Despite lacking any spindle microtubules, the defective SPB is segregated away from the functional SPB by a mechanism that re ...
... into the nuclear envelope, but instead resides on its cytoplasmic surface. Having no access to the nucleoplasm, the defective SPB cannot act as a pole of the mitotic spindle. Despite lacking any spindle microtubules, the defective SPB is segregated away from the functional SPB by a mechanism that re ...
Chapter 5
... made of a protein called spectrin links proteins embedded in the plasma membrane bilayer with actin filaments in the cell’s cytoskeleton. Membranes use networks of other proteins to control the lateral movements of some key proteins within the bilayer, anchoring them to specific sites. 4. Cell-surf ...
... made of a protein called spectrin links proteins embedded in the plasma membrane bilayer with actin filaments in the cell’s cytoskeleton. Membranes use networks of other proteins to control the lateral movements of some key proteins within the bilayer, anchoring them to specific sites. 4. Cell-surf ...
Electrophilic Affibodies Forming Covalent Bonds to Protein Targets*DS
... molecule antigens (15–17), but these strategies, such as the antibody made by Meares’ group to an electrophilic metal chelator, do not enable recognition of protein antigens composed of the regular 20 amino acids. Immunizing mice with electrophilic antigens generated an antibody that formed SDS-stab ...
... molecule antigens (15–17), but these strategies, such as the antibody made by Meares’ group to an electrophilic metal chelator, do not enable recognition of protein antigens composed of the regular 20 amino acids. Immunizing mice with electrophilic antigens generated an antibody that formed SDS-stab ...
facilitated diffusion
... (these substances are called solutes) Sometimes there is a higher concentration of solutes on one side of the membrane than the other This is called a concentration gradient: where there is difference in concentration of a substance on the inside and outside of the cell ...
... (these substances are called solutes) Sometimes there is a higher concentration of solutes on one side of the membrane than the other This is called a concentration gradient: where there is difference in concentration of a substance on the inside and outside of the cell ...
Cellular mechanisms of potassium transport in plants
... another near 11 mM (Fu and Luan 1998, Kim et al. 1998). The mechanism by which the switch between affinities occurs may involve phosphorylation of the transport protein, as has been demonstrated for the switch between affinities of the nitrate transporter CHL1 (Liu and Tsay 2003), and for the change ...
... another near 11 mM (Fu and Luan 1998, Kim et al. 1998). The mechanism by which the switch between affinities occurs may involve phosphorylation of the transport protein, as has been demonstrated for the switch between affinities of the nitrate transporter CHL1 (Liu and Tsay 2003), and for the change ...
Milk Components - Great Lakes Regional Dairy Conference
... October 6 to January 6 for fat and November 21 to December 12 for protein • Amplitude of the rhythm ranged from 0.07 to 0.14% for fat and from 0.08 to 0.12% for protein • Amplitude for fat from generally lower in southern and higher in northern • Range in fat and protein in Mideast was 0.26% for fat ...
... October 6 to January 6 for fat and November 21 to December 12 for protein • Amplitude of the rhythm ranged from 0.07 to 0.14% for fat and from 0.08 to 0.12% for protein • Amplitude for fat from generally lower in southern and higher in northern • Range in fat and protein in Mideast was 0.26% for fat ...
Protein sequence analysis
... Alignment of protein sequences can take account of the diferential rates at which amino acids substitute for each other. It can be measured through two types of matrices: PAM and BLOSUM. PAM (Percent Accepted Mutations): on the basis of comparisons among many pairs of very similar protein sequences ...
... Alignment of protein sequences can take account of the diferential rates at which amino acids substitute for each other. It can be measured through two types of matrices: PAM and BLOSUM. PAM (Percent Accepted Mutations): on the basis of comparisons among many pairs of very similar protein sequences ...
In vitro phosphorylation of the movement protein of tomato mosaic
... of ToMV (formerly designated TMV tomato strain L), was treated with Klenow fragment to fill in the 3h termini and inserted into the SmaI site of pBluescriptII SK(j) (Stratagene) to create pBS-30K, with the coding sequence in the EcoRI to NotI sense of the vector. The 1n0 kb EcoRI–NotI fragment was s ...
... of ToMV (formerly designated TMV tomato strain L), was treated with Klenow fragment to fill in the 3h termini and inserted into the SmaI site of pBluescriptII SK(j) (Stratagene) to create pBS-30K, with the coding sequence in the EcoRI to NotI sense of the vector. The 1n0 kb EcoRI–NotI fragment was s ...
Branched-Chain Amino Acids in Exercise
... The effects of leucine are, at least in part, associated with the absence of the branched-chain aminotransferase (BCAT) enzyme in liver (15). Absence of BCAT in liver results in an enriched supply of the three BCAAs channeled to skeletal muscle. Dietary BCAA reach the blood virtually unaltered from ...
... The effects of leucine are, at least in part, associated with the absence of the branched-chain aminotransferase (BCAT) enzyme in liver (15). Absence of BCAT in liver results in an enriched supply of the three BCAAs channeled to skeletal muscle. Dietary BCAA reach the blood virtually unaltered from ...
Biology - Kenyon College
... For two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2-D gels), growth media contained LBK broth (10 g of tryptone, 5 g of yeast extract, 7.45 g of KCl) or M63 salts [3 g of KH2PO4, 7 g of K2HPO4, 2 g of (NH4)2SO4, 0.5 ml of FeSO4 at 1 mg/ml, 2 ml of 0.5 M MgSO2, 100 mg of ...
... For two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2-D gels), growth media contained LBK broth (10 g of tryptone, 5 g of yeast extract, 7.45 g of KCl) or M63 salts [3 g of KH2PO4, 7 g of K2HPO4, 2 g of (NH4)2SO4, 0.5 ml of FeSO4 at 1 mg/ml, 2 ml of 0.5 M MgSO2, 100 mg of ...
Pierce Magnetic HA-Tag IP/Co-IP Kit
... Do not centrifuge, dry or freeze the Pierce Magnetic Beads. Centrifuging, drying or freezing will cause the beads to aggregate and lose binding activity. ...
... Do not centrifuge, dry or freeze the Pierce Magnetic Beads. Centrifuging, drying or freezing will cause the beads to aggregate and lose binding activity. ...
Norton J Nutr 2006
... The effects of leucine are, at least in part, associated with the absence of the branched-chain aminotransferase (BCAT) enzyme in liver (15). Absence of BCAT in liver results in an enriched supply of the three BCAAs channeled to skeletal muscle. Dietary BCAA reach the blood virtually unaltered from ...
... The effects of leucine are, at least in part, associated with the absence of the branched-chain aminotransferase (BCAT) enzyme in liver (15). Absence of BCAT in liver results in an enriched supply of the three BCAAs channeled to skeletal muscle. Dietary BCAA reach the blood virtually unaltered from ...
functional differentiation of enterocytes in the follicle
... Tritiated valine at a concentration of 1 mM was presented to the luminal surface of rat mid-jejunum for a period of 45 s, as described in the previous section. A typical autoradiograph of sectioned tissue taken in the region of a Peyer's patch is shown in Fig. 1 A. Radioactivity is seen to be concen ...
... Tritiated valine at a concentration of 1 mM was presented to the luminal surface of rat mid-jejunum for a period of 45 s, as described in the previous section. A typical autoradiograph of sectioned tissue taken in the region of a Peyer's patch is shown in Fig. 1 A. Radioactivity is seen to be concen ...
Amino Acids and Proteins Amino Acid Compound
... comparison with the migration of DNP-derivative standards allows for the identification of the N-terminal amino acid. Dansyl chloride: Like DNF, dansyl chloride reacts with the N-terminal residue under alkaline conditions. Analysis of the modified amino acids is carried out similarly to the Sanger ...
... comparison with the migration of DNP-derivative standards allows for the identification of the N-terminal amino acid. Dansyl chloride: Like DNF, dansyl chloride reacts with the N-terminal residue under alkaline conditions. Analysis of the modified amino acids is carried out similarly to the Sanger ...
IJEB 48(4) 373-377
... Thr. The earlier studies on azo-dye bound peptide revealed that it contained Pro, Leu/Ile, Val and Gly as major components and Glu, Phe, Ser, Ala and Asp as minor components19. Terayama and Takeuchi20 isolated a fraction on hydrolysis of azo-dye binding protein which was found to have Phe, Ser, Gly, ...
... Thr. The earlier studies on azo-dye bound peptide revealed that it contained Pro, Leu/Ile, Val and Gly as major components and Glu, Phe, Ser, Ala and Asp as minor components19. Terayama and Takeuchi20 isolated a fraction on hydrolysis of azo-dye binding protein which was found to have Phe, Ser, Gly, ...
Peptide microarrays for detailed, high-throughput
... reagent consumption were reduced considerably. PKA was shown to phosphorylate many peptides containing known PKA phosphorylation sites as well as some new substrates. Data in table 1 shows that staurosporine is a full ATP competitive inhibitor whereas AMP-PNP has a different inhibition mechanism. ...
... reagent consumption were reduced considerably. PKA was shown to phosphorylate many peptides containing known PKA phosphorylation sites as well as some new substrates. Data in table 1 shows that staurosporine is a full ATP competitive inhibitor whereas AMP-PNP has a different inhibition mechanism. ...
Amino acids used in Animal Nutrition
... =>Reduced cost of diets => improved utilisation of N Increase AA balance accuracy Reduce CP of diets ...
... =>Reduced cost of diets => improved utilisation of N Increase AA balance accuracy Reduce CP of diets ...
Response of Jujube Fruits to Exogenous Oxalic Acid Treatment
... that OA was available as a natural antioxidant and played an important role in the natural and artificial preservation of oxidized materials. OA has also been shown to be an antibrowning agent for harvested vegetables (Castaner et al. 1997) and banana slices (Yoruk et al. 2002). Zhang et al. (1998) o ...
... that OA was available as a natural antioxidant and played an important role in the natural and artificial preservation of oxidized materials. OA has also been shown to be an antibrowning agent for harvested vegetables (Castaner et al. 1997) and banana slices (Yoruk et al. 2002). Zhang et al. (1998) o ...
This is the accepted version of the following article:
... cerebrospinal fluid (CSF), but not in plasma or serum, have been generally accepted as sensitive AD diagnostic markers [5]. Since phosphorylation may change some physico-chemical properties and functions of protein [6, 7] it is necessary to operate under in vitro studies also with the natural struct ...
... cerebrospinal fluid (CSF), but not in plasma or serum, have been generally accepted as sensitive AD diagnostic markers [5]. Since phosphorylation may change some physico-chemical properties and functions of protein [6, 7] it is necessary to operate under in vitro studies also with the natural struct ...
Vertebrate hairy and Enhancer of split related proteins
... superfamily, although residues at certain positions are speci®c to bHLH-O proteins (see below). The intervening sequence between the bHLH and Orange domains ranges from seven to 41 amino acids. Although not shown in Figure 2, this intervening sequence is highly similar among members of the hairy, He ...
... superfamily, although residues at certain positions are speci®c to bHLH-O proteins (see below). The intervening sequence between the bHLH and Orange domains ranges from seven to 41 amino acids. Although not shown in Figure 2, this intervening sequence is highly similar among members of the hairy, He ...
Cyanidioschyzon merolae Genome. A Tool for
... Figure 1. The basic courses of mitosis in a typical eukaryotic cell and unicellular red alga (C. merolae) cell. As typical cells contain many double membrane- and single membrane-bound organelles, they are not illustrated. During interphase, the centrosome (CEN) forms outside the nucleus (N), inclu ...
... Figure 1. The basic courses of mitosis in a typical eukaryotic cell and unicellular red alga (C. merolae) cell. As typical cells contain many double membrane- and single membrane-bound organelles, they are not illustrated. During interphase, the centrosome (CEN) forms outside the nucleus (N), inclu ...
Western blot Fast purification Comparative performance
... Research. We have included articles covering both upstream and downstream processes, as well as sample preparation. There are articles comparing various techniques such as isolating small quantities of protein from complex samples; comparing differences in optical density analysis between spectropho ...
... Research. We have included articles covering both upstream and downstream processes, as well as sample preparation. There are articles comparing various techniques such as isolating small quantities of protein from complex samples; comparing differences in optical density analysis between spectropho ...
Research Applications Of Proteolytic Enzymes In Molecular Biology
... peptides, high molecular weight peptides and proteins). In this environment forcing the reaction towards peptide synthesis requires the mass action, the addition of a water-miscible organic co-solvent in high concentration or the excess of one reactant. In serine protease-catalyzed reaction in water ...
... peptides, high molecular weight peptides and proteins). In this environment forcing the reaction towards peptide synthesis requires the mass action, the addition of a water-miscible organic co-solvent in high concentration or the excess of one reactant. In serine protease-catalyzed reaction in water ...
Homology among (βα) 8 Barrels: Implications for the Evolution of
... aldolase (Mavridis et al., 1982), and shows signi®cant sequence similarity to other phosphate-binding TIM barrels (Figure 1). Comparison of known structures shows that the Schiff base-forming catalytic lysine residue is found in an exactly equivalent position in type I fructose-1,6-bisphosphate aldo ...
... aldolase (Mavridis et al., 1982), and shows signi®cant sequence similarity to other phosphate-binding TIM barrels (Figure 1). Comparison of known structures shows that the Schiff base-forming catalytic lysine residue is found in an exactly equivalent position in type I fructose-1,6-bisphosphate aldo ...
The Generic Nature of Protein Folding and Misfolding
... requires energy, and adenosine 5′-triphosphate (ATP) is required for many of the molecular chaperones to function correctly (Ellis and Hartl, 1999). Despite the fact that many molecular chaperones are usually at very high levels only in stressed systems, it is clear that they have a critical role to ...
... requires energy, and adenosine 5′-triphosphate (ATP) is required for many of the molecular chaperones to function correctly (Ellis and Hartl, 1999). Despite the fact that many molecular chaperones are usually at very high levels only in stressed systems, it is clear that they have a critical role to ...