Regulation of transcript encoding the 43K
... truncated form of the Torpedo 43K subsynaptic protein. This cDNA was generously provided by Drs Frail and Merlie (Washington University School of Medicine, St Louis, MO) and has been designated T43k.l (Frail et al. 1987). Filters were hybridized with 32P-probe in 5xSSPE, 5xDenhardt's, 100^gmP 1 calf ...
... truncated form of the Torpedo 43K subsynaptic protein. This cDNA was generously provided by Drs Frail and Merlie (Washington University School of Medicine, St Louis, MO) and has been designated T43k.l (Frail et al. 1987). Filters were hybridized with 32P-probe in 5xSSPE, 5xDenhardt's, 100^gmP 1 calf ...
Barley MLA Immune Receptors Directly Interfere
... activity of ;2.0 units (Figure 3A, left). Further conditional expression of MLA10_CC wild type or its L18E variant, but not of the CC L11E variant, dramatically reduced reporter activity to ;0.1 unit (Figure 3A, left), possibly resulting from competitive interactions (i.e., CC binding to WRKY1, MYB6 ...
... activity of ;2.0 units (Figure 3A, left). Further conditional expression of MLA10_CC wild type or its L18E variant, but not of the CC L11E variant, dramatically reduced reporter activity to ;0.1 unit (Figure 3A, left), possibly resulting from competitive interactions (i.e., CC binding to WRKY1, MYB6 ...
PLoS Pathogens
... referred as basal defense in plants, restricts the growth of the vast majority of potential pathogens encountered by plants in the surrounding environment [2,3]. However, successful pathogens produce virulence factors to effectively suppress PTI. For example, Gram-negative bacterial pathogens, such ...
... referred as basal defense in plants, restricts the growth of the vast majority of potential pathogens encountered by plants in the surrounding environment [2,3]. However, successful pathogens produce virulence factors to effectively suppress PTI. For example, Gram-negative bacterial pathogens, such ...
Elastin-like Polypeptide (ELP) Platform: Creation of Differentiated
... VIP peptide and PB1046 have been shown to have positive inotropic and lusitropic effects on the heart which, together with VIP’s anti-inflammatory and anti-fibrotic activity, peripheral and coronary vasodilation effects, indicate a potential to delay or reverse cardiomyopathy associated with DMD. Su ...
... VIP peptide and PB1046 have been shown to have positive inotropic and lusitropic effects on the heart which, together with VIP’s anti-inflammatory and anti-fibrotic activity, peripheral and coronary vasodilation effects, indicate a potential to delay or reverse cardiomyopathy associated with DMD. Su ...
Huntingtin: Its Role in Gene Expression
... Htt and the mis-spliced mRNA further by identifying the mRNA sequence that allows them to associate, identifying any other proteins that may be involved in the interaction, and determining whether the 3’ UTR sequence of the mis-spliced mRNA has a function similar to its function in the normal HTT mR ...
... Htt and the mis-spliced mRNA further by identifying the mRNA sequence that allows them to associate, identifying any other proteins that may be involved in the interaction, and determining whether the 3’ UTR sequence of the mis-spliced mRNA has a function similar to its function in the normal HTT mR ...
Identification of a Second Collagen-Like
... backbone has a predicted size of approximately 39 kDa, but the intact protein migrates with an apparent mass of ⬎250 kDa for the Sterne strain, which is consistent with its being heavily glycosylated (30). It is entirely possible that there may be additional protein modifications. The genetic region ...
... backbone has a predicted size of approximately 39 kDa, but the intact protein migrates with an apparent mass of ⬎250 kDa for the Sterne strain, which is consistent with its being heavily glycosylated (30). It is entirely possible that there may be additional protein modifications. The genetic region ...
AKAP MEDIATED SIGNAL TRANSDUCTION Jennifer J. Carlisle
... basis of their ability to bind to PKA and coprecipitate catalytic activity (Figure 1). All contain an amphipathic helix of 14 to 18 residues that functions to bind to the N-termini of the PKA-RII dimer (25–27). Each AKAP also contains a unique subcellular targeting domain that restricts its location ...
... basis of their ability to bind to PKA and coprecipitate catalytic activity (Figure 1). All contain an amphipathic helix of 14 to 18 residues that functions to bind to the N-termini of the PKA-RII dimer (25–27). Each AKAP also contains a unique subcellular targeting domain that restricts its location ...
Interaction of Graphene Oxide with Proteins and
... on graphene oxide sheets functionalized by BSA is enabled by a specific chemical bonding between bovine serum albumin and metal nanoparticles due to the presence of thiol, amine, and imidazole groups in BSA (i.e., cysteine, lysine and histidine residues) where the hydrophobic and electrostatic inter ...
... on graphene oxide sheets functionalized by BSA is enabled by a specific chemical bonding between bovine serum albumin and metal nanoparticles due to the presence of thiol, amine, and imidazole groups in BSA (i.e., cysteine, lysine and histidine residues) where the hydrophobic and electrostatic inter ...
Hypothesis review: are clathrin-mediated endocytosis and clathrin
... adaptor protein complex 2 (AP-2) at endocytic membrane sites containing cargo proteins. The sites then form clathrin-coated pits, which are separated from the membrane by scission protein dynamin and its associated machinery. The clathrin-coated vesicle (CCV) is transported intracellularly along act ...
... adaptor protein complex 2 (AP-2) at endocytic membrane sites containing cargo proteins. The sites then form clathrin-coated pits, which are separated from the membrane by scission protein dynamin and its associated machinery. The clathrin-coated vesicle (CCV) is transported intracellularly along act ...
Batesian Mimicry: Can a Leopard Change Its Spots — and Get
... plasticine dummy snakes placed in the wild. As expected from theory, the advantages of mimicry in sympatry become disadvantages in the absence of models, in which case patterns with less black banding are favoured. But the mimic’s pattern does not revert to an ancestral kingsnake pattern; instead, i ...
... plasticine dummy snakes placed in the wild. As expected from theory, the advantages of mimicry in sympatry become disadvantages in the absence of models, in which case patterns with less black banding are favoured. But the mimic’s pattern does not revert to an ancestral kingsnake pattern; instead, i ...
Heterogeneous chromatin mobility derived from chromatin
... The three RNA polymerase II subunits present in the ChIP dataset, Rpb2p, Rpb3p and Rpb7p (Fig. 2A), display different binding patterns across chromatin states, and their fraction occupied values per state are shown in Fig. 2B. Interpretation is made easier when assessing the rank protein occupancy ( ...
... The three RNA polymerase II subunits present in the ChIP dataset, Rpb2p, Rpb3p and Rpb7p (Fig. 2A), display different binding patterns across chromatin states, and their fraction occupied values per state are shown in Fig. 2B. Interpretation is made easier when assessing the rank protein occupancy ( ...
The chimeric leucine-rich repeat/extensin cell wall protein
... In plants, the cell wall is a major determinant of cell morphogenesis. Cell enlargement depends on the tightly regulated expansion of the wall, which surrounds each cell. However, the qualitative and quantitative mechanisms controlling cell wall enlargement are still poorly understood. Here, we repo ...
... In plants, the cell wall is a major determinant of cell morphogenesis. Cell enlargement depends on the tightly regulated expansion of the wall, which surrounds each cell. However, the qualitative and quantitative mechanisms controlling cell wall enlargement are still poorly understood. Here, we repo ...
Functional characterization of dosage‐dependent lethal mutation of
... the contribution of these invariant amino acid residues to structural and functional topology of the molecule we have utilized in vitro evolution of the protein using errorprone PCR (Prabha et al., 2010). The resultant mutants were screened in the SUB60 strain of Saccharomyces cerevisiae. In S. cere ...
... the contribution of these invariant amino acid residues to structural and functional topology of the molecule we have utilized in vitro evolution of the protein using errorprone PCR (Prabha et al., 2010). The resultant mutants were screened in the SUB60 strain of Saccharomyces cerevisiae. In S. cere ...
A biofilm-forming marine bacterium producing proteins
... another cell bound fraction, non soluble in a salt solution (36g/l). None of them is pure. EPS1 consists mainly in a majority of carbohydrates while the two others consist mainly in a majority of proteins. Gel electrophoresis analysis showed different EPS proteins composition with molecular weight ...
... another cell bound fraction, non soluble in a salt solution (36g/l). None of them is pure. EPS1 consists mainly in a majority of carbohydrates while the two others consist mainly in a majority of proteins. Gel electrophoresis analysis showed different EPS proteins composition with molecular weight ...
GFP is the way to glow: bioimaging of the plant endomembrane
... and microfilaments labelled with fluorescent proteins. 2.3. Golgi imaging in vivo Studies on the dynamics of the plant Golgi are relatively young in comparison with their mammalian counterparts, due to the absence of genuine plant Golgi enzymes to be fused to GFP and used as in vivo markers. The fir ...
... and microfilaments labelled with fluorescent proteins. 2.3. Golgi imaging in vivo Studies on the dynamics of the plant Golgi are relatively young in comparison with their mammalian counterparts, due to the absence of genuine plant Golgi enzymes to be fused to GFP and used as in vivo markers. The fir ...
Plant–pathogen interactions: what is proteomics telling us?
... and protein function and expression profiles. Using these ‘omic’ approaches, pathogenicity- and defence-related genes and proteins expressed during phytopathogen infections have been identified and enormous datasets have been accumulated. However, the understanding of molecular plant–pathogen interact ...
... and protein function and expression profiles. Using these ‘omic’ approaches, pathogenicity- and defence-related genes and proteins expressed during phytopathogen infections have been identified and enormous datasets have been accumulated. However, the understanding of molecular plant–pathogen interact ...
Abscisic Acid–Induced Transcription Is Mediated by
... and ABI5 also has been demonstrated by yeast two-hybrid assays (Nakamura et al., 2001). In addition to its function in late embryogenesis, ABI5 is suggested to play a role in a checkpoint mechanism by which growth is arrested under stressed conditions during a limited period of postgerminative devel ...
... and ABI5 also has been demonstrated by yeast two-hybrid assays (Nakamura et al., 2001). In addition to its function in late embryogenesis, ABI5 is suggested to play a role in a checkpoint mechanism by which growth is arrested under stressed conditions during a limited period of postgerminative devel ...
the scf ubiquitin ligase: insights into a molecular machine
... (LRR)), the domain is an arc-shaped α–β-repeat structure that is also found in many protein-binding contexts, including the extracellular-binding domain of certain surface receptors11,12. In most cases, FBLs also seem to involve substrate phosphorylation for their interaction, but this does not seem ...
... (LRR)), the domain is an arc-shaped α–β-repeat structure that is also found in many protein-binding contexts, including the extracellular-binding domain of certain surface receptors11,12. In most cases, FBLs also seem to involve substrate phosphorylation for their interaction, but this does not seem ...
Similarities and Differences in the Glycosylation Mechanisms in
... have shown that PglK (previously called wlaB), which is an ABC-type transporter, is responsible for flipping the LLO [14]. Interestingly, these workers found that PglK has a relaxed substrate specificity exemplified by its ability to complement a Wzx deficiency in O-antigen biosynthesis in E. coli. ...
... have shown that PglK (previously called wlaB), which is an ABC-type transporter, is responsible for flipping the LLO [14]. Interestingly, these workers found that PglK has a relaxed substrate specificity exemplified by its ability to complement a Wzx deficiency in O-antigen biosynthesis in E. coli. ...
Sample preparation and analytical strategies for
... Protein phosphorylation is a highly dynamic modification regulated by an intricate network of kinases and phosphatases. Upon extrinsic and intrinsic stimuli, proteins can be phosphorylated and dephosphorylated within minutes, and protease and phosphatase inhibitors must be used during cell lysis and ...
... Protein phosphorylation is a highly dynamic modification regulated by an intricate network of kinases and phosphatases. Upon extrinsic and intrinsic stimuli, proteins can be phosphorylated and dephosphorylated within minutes, and protease and phosphatase inhibitors must be used during cell lysis and ...
Structural Insights into Triglyceride Storage Mediated by
... triglyceride biosynthesis. FIT proteins do not share primary sequence homology with known proteins and no structural information is available to inform on the mechanism by which FIT proteins function. Here, we present the experimentallysolved topological models for FIT1 and FIT2 using N-glycosylatio ...
... triglyceride biosynthesis. FIT proteins do not share primary sequence homology with known proteins and no structural information is available to inform on the mechanism by which FIT proteins function. Here, we present the experimentallysolved topological models for FIT1 and FIT2 using N-glycosylatio ...
Sample & Assay Technologies QIAgenes E. coli
... QIAgenes expression constructs are based on the T7 promoter-driven pQE-T7 family of vectors. In pQE-T7 vectors, target genes are cloned into positions under the control of strong bacteriophage T7 transcription and translation signals, and expression is induced by providing a source of T7 RNA polymer ...
... QIAgenes expression constructs are based on the T7 promoter-driven pQE-T7 family of vectors. In pQE-T7 vectors, target genes are cloned into positions under the control of strong bacteriophage T7 transcription and translation signals, and expression is induced by providing a source of T7 RNA polymer ...
The twin arginine protein transport pathway exports multiple
... undergoes complex morphological differentiation involving the formation of aerial hyphae and spores (see Flärdh and Buttner, 2009 for a review of the complex biology of Streptomyces). The hyphal form of S. scabies infects plants through expanding plant tissue, including roots and tubers (Loria et al ...
... undergoes complex morphological differentiation involving the formation of aerial hyphae and spores (see Flärdh and Buttner, 2009 for a review of the complex biology of Streptomyces). The hyphal form of S. scabies infects plants through expanding plant tissue, including roots and tubers (Loria et al ...