The twin arginine protein transport pathway exports multiple
... undergoes complex morphological differentiation involving the formation of aerial hyphae and spores (see Flärdh and Buttner, 2009 for a review of the complex biology of Streptomyces). The hyphal form of S. scabies infects plants through expanding plant tissue, including roots and tubers (Loria et al ...
... undergoes complex morphological differentiation involving the formation of aerial hyphae and spores (see Flärdh and Buttner, 2009 for a review of the complex biology of Streptomyces). The hyphal form of S. scabies infects plants through expanding plant tissue, including roots and tubers (Loria et al ...
The role of aquaporins in cellular and whole plant water balance
... that is, the movement of water molecules across the membrane which occurs even in the absence of a gradient. Pf and Pd are intrinsic properties of each membrane. In order to distinguish between lipid- and channel-mediated water £ow, the values of Pf and Pd can be used. In a membrane where the water ...
... that is, the movement of water molecules across the membrane which occurs even in the absence of a gradient. Pf and Pd are intrinsic properties of each membrane. In order to distinguish between lipid- and channel-mediated water £ow, the values of Pf and Pd can be used. In a membrane where the water ...
CHAPTER 6 Gene Expression: Translation
... the P site. Eukaryotes use a similar process, with a factor called eEF-2. 2. Release of the uncharged tRNA involves the 50S ribosomal E (for Exit) site. Binding of a charged tRNA in the A site is blocked until the spent tRNA is released from the E site. 3. During translocation the peptidyl-tRNA rema ...
... the P site. Eukaryotes use a similar process, with a factor called eEF-2. 2. Release of the uncharged tRNA involves the 50S ribosomal E (for Exit) site. Binding of a charged tRNA in the A site is blocked until the spent tRNA is released from the E site. 3. During translocation the peptidyl-tRNA rema ...
Sample & Assay Technologies QIAgenes E. coli
... QIAgenes expression constructs are based on the T7 promoter-driven pQE-T7 family of vectors. In pQE-T7 vectors, target genes are cloned into positions under the control of strong bacteriophage T7 transcription and translation signals, and expression is induced by providing a source of T7 RNA polymer ...
... QIAgenes expression constructs are based on the T7 promoter-driven pQE-T7 family of vectors. In pQE-T7 vectors, target genes are cloned into positions under the control of strong bacteriophage T7 transcription and translation signals, and expression is induced by providing a source of T7 RNA polymer ...
Non-Essential Amino Acids
... which carries them to liver. They are then transported throughout the body to various organs that need protein replenishment. If too much protein, it will not be absorbed and will continue to large intestine and kidneys = excreted in urine ...
... which carries them to liver. They are then transported throughout the body to various organs that need protein replenishment. If too much protein, it will not be absorbed and will continue to large intestine and kidneys = excreted in urine ...
Mechanism of isoniazid uptake in Mycobacterium
... KatG then leads to the accumulation of radioactive derivatives inside the cells. A consequence of this model is that the initial transport velocity of INH is independent of KatG, but labelling at the uptake plateau depends on the peroxidase activity. In the above experiments, we did observe effects ...
... KatG then leads to the accumulation of radioactive derivatives inside the cells. A consequence of this model is that the initial transport velocity of INH is independent of KatG, but labelling at the uptake plateau depends on the peroxidase activity. In the above experiments, we did observe effects ...
University of Groningen Expression and engineering of
... In Chapter 3 a sandwiched-culture technique is described, which can be used to screen filamentous fungi for production of heterologous proteins irrespective of proteases in the medium. This technique involves culturing of transformants between perforated polycarbonate membranes and transfer of this ...
... In Chapter 3 a sandwiched-culture technique is described, which can be used to screen filamentous fungi for production of heterologous proteins irrespective of proteases in the medium. This technique involves culturing of transformants between perforated polycarbonate membranes and transfer of this ...
Isolation and Characterization of Plastidic Glucose-6-Phosphate Dehydrogenase
... source for ADPG synthesis in most plastids. Nonphotosynthetic tissues lack the capacity to convert triose phosphate to hexose phosphate (Entwistle and ap Rees, 1990) but do have the ability to incorporate metabolites into starch in isolated plastids. The metabolite glucose-6-phosphate (G-6-P) is uti ...
... source for ADPG synthesis in most plastids. Nonphotosynthetic tissues lack the capacity to convert triose phosphate to hexose phosphate (Entwistle and ap Rees, 1990) but do have the ability to incorporate metabolites into starch in isolated plastids. The metabolite glucose-6-phosphate (G-6-P) is uti ...
Storage globulins pass through the Golgi apparatus and
... 1368 | Wang et al. the Golgi apparatus where they begin to aggregate at the periphery of the cis-cisternae (Müntz, 1998; Hillmer et al., 2001; Vitale and Hinz, 2005). This condensation-based sorting leads to the formation of so-called ‘dense vesicles’ (DVs) which are released from the trans-Golgi ...
... 1368 | Wang et al. the Golgi apparatus where they begin to aggregate at the periphery of the cis-cisternae (Müntz, 1998; Hillmer et al., 2001; Vitale and Hinz, 2005). This condensation-based sorting leads to the formation of so-called ‘dense vesicles’ (DVs) which are released from the trans-Golgi ...
Proti-Ace Kit - Hampton Research
... 4. Set the crystallization experiment using the protease:sample mixture. Optimization of In Situ Proteolysis for Crystallization a. Vary the protease:sample ratio. Typical protease:sample ratios are 1:100, 1:1,000 and 1:10,000. b. Alter the incubation time. Typical incubation times are between 0 and ...
... 4. Set the crystallization experiment using the protease:sample mixture. Optimization of In Situ Proteolysis for Crystallization a. Vary the protease:sample ratio. Typical protease:sample ratios are 1:100, 1:1,000 and 1:10,000. b. Alter the incubation time. Typical incubation times are between 0 and ...
Separation and analysis of amino acids
... Electrophoresis • Electrophoresis is a technique that uses the net charge of peptides (amino acids) as a basis for separation. A potential difference is applied across a solid material (e.g. paper for amino acid analysis) permeated by an electrolyte. ...
... Electrophoresis • Electrophoresis is a technique that uses the net charge of peptides (amino acids) as a basis for separation. A potential difference is applied across a solid material (e.g. paper for amino acid analysis) permeated by an electrolyte. ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... proteins of pumpkin, namely, 11S globulin and 2S albumin, are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessar ...
... proteins of pumpkin, namely, 11S globulin and 2S albumin, are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessar ...
Stress puts TIA on TOP
... 59TOP mRNAs are regulated in a tissue- and cell typespecific manner. For example, eEF2 mRNA confers growthdependent regulation in cells of hematopoietic, but not in cells of nonhematopoietic, origins, while translation of b1-tubulin mRNA, which possesses all classical features of TOP mRNAs, is absol ...
... 59TOP mRNAs are regulated in a tissue- and cell typespecific manner. For example, eEF2 mRNA confers growthdependent regulation in cells of hematopoietic, but not in cells of nonhematopoietic, origins, while translation of b1-tubulin mRNA, which possesses all classical features of TOP mRNAs, is absol ...
Polypeptide Composition of Envelopes of Spinach Chloroplasts
... Preparation of intact chloroplasts—Spinach was purchased from a local farmer. Intact chloroplasts were prepared according to Siegenthaler and Dumont (1990) with some modifications. Leaves were homogenized for 10 s in the grinding medium containing 350 mM sorbitol, 25 mM 3-(/V-morpholino)propanesufon ...
... Preparation of intact chloroplasts—Spinach was purchased from a local farmer. Intact chloroplasts were prepared according to Siegenthaler and Dumont (1990) with some modifications. Leaves were homogenized for 10 s in the grinding medium containing 350 mM sorbitol, 25 mM 3-(/V-morpholino)propanesufon ...
CHAPTER 6 Gene Expression: Translation
... phage with proflavin. Proflavin causes frameshift mutants by inserting or deleting base pairs of DNA. 4. Crick and colleagues(1961) reasoned that reversion of a deletion (a – mutation) could be caused by a nearby insertion (a + mutation) , and vice versa. Revertants of rII to r+ can be detected by p ...
... phage with proflavin. Proflavin causes frameshift mutants by inserting or deleting base pairs of DNA. 4. Crick and colleagues(1961) reasoned that reversion of a deletion (a – mutation) could be caused by a nearby insertion (a + mutation) , and vice versa. Revertants of rII to r+ can be detected by p ...
to get the file - Chair of Computational Biology
... - Insertion of TM proteins into membrane: Translocon, MINS (today, V1) - Prediction of TM segments from sequence (V2) - Composition of Lipid membrane, Phase transitions (V3) - Elasticity of membranes (V4) - Predicting lipid-facing helix faces from sequence: TMX (V5) - Predicting helix interactions f ...
... - Insertion of TM proteins into membrane: Translocon, MINS (today, V1) - Prediction of TM segments from sequence (V2) - Composition of Lipid membrane, Phase transitions (V3) - Elasticity of membranes (V4) - Predicting lipid-facing helix faces from sequence: TMX (V5) - Predicting helix interactions f ...
novel therapy of high-priority citrus diseases
... in citrus1. Although known for a century in East Asia, it was only recently that HLB was introduced into the Americas, including the United States and Brazil, two of the top citrusproducing nations2. Typical HLB symptoms are leaves with blotchy mottling. HLB shortens the lifespan of the trees and re ...
... in citrus1. Although known for a century in East Asia, it was only recently that HLB was introduced into the Americas, including the United States and Brazil, two of the top citrusproducing nations2. Typical HLB symptoms are leaves with blotchy mottling. HLB shortens the lifespan of the trees and re ...
Text - Enlighten - University of Glasgow
... whether suppressing H+ -ATPase activity at the plasma membrane is essential for stomatal closure and how it affects K+ transport, and more generally osmotic solute transport, at both the plasma membrane and tonoplast. The elements of an OnGuard model Stomatal movements arise from the transport, accu ...
... whether suppressing H+ -ATPase activity at the plasma membrane is essential for stomatal closure and how it affects K+ transport, and more generally osmotic solute transport, at both the plasma membrane and tonoplast. The elements of an OnGuard model Stomatal movements arise from the transport, accu ...
What is Xtend
... phosphatodyl-inositol-3-kinase pathway (PI3K). PI3K regulates glucose uptake through GLUT4 translocation and also increases amino acid uptake. Insulin, the body's "storage" hormone, works by activating the PI3K pathway. Interestingly, leucine ingestion causes insulin secretion, but leucine can also ...
... phosphatodyl-inositol-3-kinase pathway (PI3K). PI3K regulates glucose uptake through GLUT4 translocation and also increases amino acid uptake. Insulin, the body's "storage" hormone, works by activating the PI3K pathway. Interestingly, leucine ingestion causes insulin secretion, but leucine can also ...
Glycosylation and Sorting of Secretory Proteins in the Endoplasmic
... 2.1.2 The sorting signal resides in the C-terminal domain of Hsp150 .. 46 2.1.3 Hsp150∆-HRP fusion protein localizes to the ER .....................47 2.1.4 The Hsp150 sorting signal actively mediates ER exit of invertase ...........................................47 2.2 Role of the Sec24p homologue ...
... 2.1.2 The sorting signal resides in the C-terminal domain of Hsp150 .. 46 2.1.3 Hsp150∆-HRP fusion protein localizes to the ER .....................47 2.1.4 The Hsp150 sorting signal actively mediates ER exit of invertase ...........................................47 2.2 Role of the Sec24p homologue ...
Transcriptomics of In Vitro Immune-Stimulated Hemocytes
... properties. In addition, it provides nutrition through its digestive properties as it is a hydrolytic protein that can break the glycosidic union of the peptidoglycans of the bacteria cell wall [24]. The C1q domain containing proteins are a family of proteins that form part of the complement system. ...
... properties. In addition, it provides nutrition through its digestive properties as it is a hydrolytic protein that can break the glycosidic union of the peptidoglycans of the bacteria cell wall [24]. The C1q domain containing proteins are a family of proteins that form part of the complement system. ...