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... With reference to Table 4.2, and the information in (iii), state the cause or causes of reduced fertility in these mutant worms at each temperature. ...
... With reference to Table 4.2, and the information in (iii), state the cause or causes of reduced fertility in these mutant worms at each temperature. ...
Optimizing cofactor availability for the production of recombinant
... production landscapes from microscale cultivations using 1 mM FeSO4-supplemented medium. Error bars are standard deviations from all measured clones of a landscape. Average activity from strains producing HRP alone was set to 100%. In addition to strong constitutive co-overexpression of either HEM1 ...
... production landscapes from microscale cultivations using 1 mM FeSO4-supplemented medium. Error bars are standard deviations from all measured clones of a landscape. Average activity from strains producing HRP alone was set to 100%. In addition to strong constitutive co-overexpression of either HEM1 ...
161021 NGF revised Manuscript with figs
... antibodies with poor solubility or high aggregation propensity to ameliorate these characteristics 20- ...
... antibodies with poor solubility or high aggregation propensity to ameliorate these characteristics 20- ...
EXPRESSION OF EQUIVALENT CLONOTYPES IN BALB/c
... positions in these sequencer runs were initially analyzed by GC and results from the H chain are given in Table II. As may be seen at position 32 the total yield of Phe is 4.7 nM. 8% of the total sample was used for GC analysis or approximately 0.4 nM of Phe. This amount of material is close to the ...
... positions in these sequencer runs were initially analyzed by GC and results from the H chain are given in Table II. As may be seen at position 32 the total yield of Phe is 4.7 nM. 8% of the total sample was used for GC analysis or approximately 0.4 nM of Phe. This amount of material is close to the ...
Lateral relocation of auxin efflux regulator PIN3 mediates tropism in
... Figure 2 pin3 mutants. a, Exon/intron organization of PIN3 on chromosome 1 showing positions of En-1 elements in pin3-1 and -2 alleles. b, c, pin3 hypocotyls are defective in gravitropic (b) as well as phototropic (c) responses. Scale bars, 500 mm. d, pin3 mutants are defective in root gravitropism. ...
... Figure 2 pin3 mutants. a, Exon/intron organization of PIN3 on chromosome 1 showing positions of En-1 elements in pin3-1 and -2 alleles. b, c, pin3 hypocotyls are defective in gravitropic (b) as well as phototropic (c) responses. Scale bars, 500 mm. d, pin3 mutants are defective in root gravitropism. ...
Protein transport across the endoplasmic reticulum membrane
... SecA) likely weakens interactions that keep the plug in the center of the Sec61 ⁄ SecY molecule, as indicated by an increased ion conductance when nontranslating ribosomes are bound to the channel [7]. Next, the hydrophobic segment of a signal sequence intercalates into the lateral gate, between TM2 ...
... SecA) likely weakens interactions that keep the plug in the center of the Sec61 ⁄ SecY molecule, as indicated by an increased ion conductance when nontranslating ribosomes are bound to the channel [7]. Next, the hydrophobic segment of a signal sequence intercalates into the lateral gate, between TM2 ...
¹⁵N Heteronuclear Single Quantum Coherence
... (sw Mb) in which the amino and carboxyl termini have been linked by 16 amino acids, (GlyGlyGlySer)4. New termini have been generated between the G and H helices. This permutant was shown to be 5.2 kcal/mol less stable than the wild-type protein. Backbone dynamic studies of permuted globins via 15N H ...
... (sw Mb) in which the amino and carboxyl termini have been linked by 16 amino acids, (GlyGlyGlySer)4. New termini have been generated between the G and H helices. This permutant was shown to be 5.2 kcal/mol less stable than the wild-type protein. Backbone dynamic studies of permuted globins via 15N H ...
PDF - SAGE Journals
... polymers of one or more protein subunits referred to as flagellin.3 The genome annotation for M. jannaschii indicates genes encoding three flagellin proteins (FlaB1, FlaB2 ...
... polymers of one or more protein subunits referred to as flagellin.3 The genome annotation for M. jannaschii indicates genes encoding three flagellin proteins (FlaB1, FlaB2 ...
Communication - Department of Molecular Physiology and Biophysics
... syntrophin triplet suggesting that all the syntrophins including α- as well as β-type may directly bind to dystrophin (Fig. 2). Previous work by Ozawa's group indicated that one of 43-DAGs was overlaid by dystrophin fusion protein containing cysteinerich and C-terminal domains (16). This interaction ...
... syntrophin triplet suggesting that all the syntrophins including α- as well as β-type may directly bind to dystrophin (Fig. 2). Previous work by Ozawa's group indicated that one of 43-DAGs was overlaid by dystrophin fusion protein containing cysteinerich and C-terminal domains (16). This interaction ...
4
... differences in intracellular TAG content between wild-type and espG5 mutant strains. These data show that ESX-5 has no major effect on intracellular TAG levels, suggesting that ESX-5-dependent import of extracellular fatty acids does not contribute to the accumulation of TAG under the conditions tes ...
... differences in intracellular TAG content between wild-type and espG5 mutant strains. These data show that ESX-5 has no major effect on intracellular TAG levels, suggesting that ESX-5-dependent import of extracellular fatty acids does not contribute to the accumulation of TAG under the conditions tes ...
Differential Accumulation Pattern of Met-rich beta
... callus after 6-weeks and they are regenerated into plantlets ...
... callus after 6-weeks and they are regenerated into plantlets ...
Title Optimization of Amino Acid Parameters for Correspondence of
... parvalbumin (1CPV); hemoglobina-chain, (3-chain(2MHB), and myoglobin (2MBN) for the globin family; elastase (IEST), beta-trypsin (2PTN), subtilisin (1SBT), and proteinase b (2SGB) for the serine protease; hen egg-white lysozyme (2LYZ) and bacteriophage T4 lysozyme (1LZM); trypsin inhibitor (3PTI) an ...
... parvalbumin (1CPV); hemoglobina-chain, (3-chain(2MHB), and myoglobin (2MBN) for the globin family; elastase (IEST), beta-trypsin (2PTN), subtilisin (1SBT), and proteinase b (2SGB) for the serine protease; hen egg-white lysozyme (2LYZ) and bacteriophage T4 lysozyme (1LZM); trypsin inhibitor (3PTI) an ...
N-Methylated Amino Acids
... of asparagine and glutamine residues Nγ-Methylated asparagine residues are present at position 72 of the β-subunit of many phycobilins. Together with the α-subunit they form the structural building blocks of phycobilisomes. These are multimeric complexes which harvest and transfer light energy to ph ...
... of asparagine and glutamine residues Nγ-Methylated asparagine residues are present at position 72 of the β-subunit of many phycobilins. Together with the α-subunit they form the structural building blocks of phycobilisomes. These are multimeric complexes which harvest and transfer light energy to ph ...
Partial Purification and Characterization of the Maize Mitochondrial
... two proteins. The N-terminal amino acid sequence of the 52-kD protein (Fig. 4) had the highest similarity to a mammalian dihydrolipoamide transacetylase (E2), according to BLAST (Altschul et al., 1990), an amino acid alignment algorithm. N-terminal sequencing of the 53-kD protein revealed that it is ...
... two proteins. The N-terminal amino acid sequence of the 52-kD protein (Fig. 4) had the highest similarity to a mammalian dihydrolipoamide transacetylase (E2), according to BLAST (Altschul et al., 1990), an amino acid alignment algorithm. N-terminal sequencing of the 53-kD protein revealed that it is ...
Structural Influences: Cholesterol, Drug, and Proton Binding to Full
... Isotopically labeled M2FL protein was expressed with N-terminal histidine-6 tag from the BL21 RP codon plus Escherichia coli. Initial cultures were grown in rich media. Two liters of cells were pelleted and washed with 50 mL of M9 minimal media before growing 1 L of M9 culture supplemented with a 10 ...
... Isotopically labeled M2FL protein was expressed with N-terminal histidine-6 tag from the BL21 RP codon plus Escherichia coli. Initial cultures were grown in rich media. Two liters of cells were pelleted and washed with 50 mL of M9 minimal media before growing 1 L of M9 culture supplemented with a 10 ...
Document
... In 1991, at the EU meeting on genome programs in Elounda, Greece, the presentation of the yeast chromosome III and the first 100 kb of the Bacillus subtilis genome revealed that, contrary to expectation (the only cases where this had been observed were phages, for obvious reasons), at least half of ...
... In 1991, at the EU meeting on genome programs in Elounda, Greece, the presentation of the yeast chromosome III and the first 100 kb of the Bacillus subtilis genome revealed that, contrary to expectation (the only cases where this had been observed were phages, for obvious reasons), at least half of ...
The potato tuber mitochondrial proteome
... specific set of conditions (Millar et al. 2005, Millar et al., 2006, Cui et al., 2011). To ...
... specific set of conditions (Millar et al. 2005, Millar et al., 2006, Cui et al., 2011). To ...
Characterization of Lipid Rafts from Medicago
... We used aeroponic cultures of M. truncatula plants and the conventional phase-partition procedure to obtain PM-enriched fraction (approximately 500–700 mg protein per 150 g root fresh weight). The purity of the PM fraction was first checked with marker activities to evaluate the contamination by oth ...
... We used aeroponic cultures of M. truncatula plants and the conventional phase-partition procedure to obtain PM-enriched fraction (approximately 500–700 mg protein per 150 g root fresh weight). The purity of the PM fraction was first checked with marker activities to evaluate the contamination by oth ...
Periplasmic folding factors in Gram-negative bacteria
... Summary for laymen All organisms need a system to regulate the uptake of substances from their external milieu, allowing nutrients to enter and preventing toxic substances from doing so. In Gram-negative bacteria the cell envelope is responsible for determining which substances can enter the cell. ...
... Summary for laymen All organisms need a system to regulate the uptake of substances from their external milieu, allowing nutrients to enter and preventing toxic substances from doing so. In Gram-negative bacteria the cell envelope is responsible for determining which substances can enter the cell. ...
Intersubunit contacts are often facilitated by specificity
... specificity Results obtained for several protein families of different functional type agree with structural and experimental data A substantial fraction of SDPs are located on the intersubunit contacts interface, where they form distinct spatial clasps ...
... specificity Results obtained for several protein families of different functional type agree with structural and experimental data A substantial fraction of SDPs are located on the intersubunit contacts interface, where they form distinct spatial clasps ...
Biochemical and Cellular Investigation of Vitreoscilla Hemoglobin
... were in enormous excess compared with VHb concentration to ensure the pseudo-first-order reaction. A 1-ml reaction mixture in 50 mM sodium phosphate buffer, pH 7.5 containing 0.5 µM VHb proteins, 3-75 mM H2O2, and 100-500 µM ABTS was used. Triplicate measurements were performed for each point of sub ...
... were in enormous excess compared with VHb concentration to ensure the pseudo-first-order reaction. A 1-ml reaction mixture in 50 mM sodium phosphate buffer, pH 7.5 containing 0.5 µM VHb proteins, 3-75 mM H2O2, and 100-500 µM ABTS was used. Triplicate measurements were performed for each point of sub ...
Fluorescence Study of Bovine β-Lactoglobulin
... the structure surrounding it while the third one is is generated by the interaction between Trp residue(s) and neighboring amino acids. Also, one should consider tryptophan structure and properties as different from those of NATA and indole. Finally, as it is the case for tryptophan free in solution ...
... the structure surrounding it while the third one is is generated by the interaction between Trp residue(s) and neighboring amino acids. Also, one should consider tryptophan structure and properties as different from those of NATA and indole. Finally, as it is the case for tryptophan free in solution ...
Regulation of transcript encoding the 43K
... truncated form of the Torpedo 43K subsynaptic protein. This cDNA was generously provided by Drs Frail and Merlie (Washington University School of Medicine, St Louis, MO) and has been designated T43k.l (Frail et al. 1987). Filters were hybridized with 32P-probe in 5xSSPE, 5xDenhardt's, 100^gmP 1 calf ...
... truncated form of the Torpedo 43K subsynaptic protein. This cDNA was generously provided by Drs Frail and Merlie (Washington University School of Medicine, St Louis, MO) and has been designated T43k.l (Frail et al. 1987). Filters were hybridized with 32P-probe in 5xSSPE, 5xDenhardt's, 100^gmP 1 calf ...
Responses of Growing Broilers to Diets with Increased Sulfur Amino
... TFD SAA:Lys ratio might be higher than 77%, particularly for feed conversion. Comparing the regression coefficients revealed that the steepness was higher for weight gain compared with feed conversion, which means that maximum BW gain was obtained at a lower dietary TFD SAA than needed to minimize t ...
... TFD SAA:Lys ratio might be higher than 77%, particularly for feed conversion. Comparing the regression coefficients revealed that the steepness was higher for weight gain compared with feed conversion, which means that maximum BW gain was obtained at a lower dietary TFD SAA than needed to minimize t ...